Publicação
Spatial patterns of genetic variation in the Iberian honey bee hybrid zone: a comparison between mitochondrial and nuclear DNA
| Resumo: | The Iberian honey bee (Apis mellifera iberiensis) has been intensely surveyed for genetic variation with different markers ranging from morphology, allozymes, mitochondrial DNA (mt DNA), to microsatellites. Some of these markers have revealed non-concordant complex patterns of genetic variation, which led scientists to evoke competing hypotheses for the origin of Iberian honey bees. While complex patterns and underlying historical processes are typical of hybrid zones, the use of more powerful molecular and analytical tools and the fine-scale sampling promised to help dissecting the complexities of the Iberian hybrid zone. In this study, we conducted a genome-wide sampling by genotyping over 384 SNPs (single nucleotide polymorphisms) and sequenced an intergenic fragment of the mt DNA in 711 georeferenced honey bee samples collected across three North-South transects in the Iberian Peninsula. Both mt DNA and SNP datasets were analyzed using spatial tools to represent the structure generated by both types of molecular markers. We found concordant spatial patterns between markers which led to rejection of the standing hypothesis of recent human introductions and selection as the processes shaping Iberian honey bees patterns. This study shows that the fine-scale genomic and spatial analyses can reveal patterns which would otherwise had been undetected. |
|---|---|
| Autores principais: | Pinto, M. Alice |
| Outros Autores: | Chávez-Galarza, Julio; Johnston, J. Spencer; Henriques, Dora; Rufino, José; Muñoz, Irene; De la Rúa, Pilar; Azevedo, João |
| Assunto: | Abelha Ibérica Apis mellifera iberiensis mtDNA SNPs Landscape genetics Spatial analysis |
| Ano: | 2012 |
| País: | Portugal |
| Tipo de documento: | póster em conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867173196568985600 |
|---|---|
| author | Pinto, M. Alice |
| author2 | Chávez-Galarza, Julio Johnston, J. Spencer Henriques, Dora Rufino, José Muñoz, Irene De la Rúa, Pilar Azevedo, João |
| author2_role | author author author author author author author |
| author_facet | Pinto, M. Alice Chávez-Galarza, Julio Johnston, J. Spencer Henriques, Dora Rufino, José Muñoz, Irene De la Rúa, Pilar Azevedo, João |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Pinto, M. Alice\",\"Person.identifier.orcid\":\"0000-0001-9663-8399\"},{\"Person.name\":\"Chávez-Galarza, Julio\"},{\"Person.name\":\"Johnston, J. Spencer\"},{\"Person.name\":\"Henriques, Dora\",\"Person.identifier.orcid\":\"0000-0001-7530-682X\"},{\"Person.name\":\"Rufino, José\",\"Person.identifier.orcid\":\"0000-0002-1344-8264\"},{\"Person.name\":\"Muñoz, Irene\"},{\"Person.name\":\"De la Rúa, Pilar\"},{\"Person.name\":\"Azevedo, João\",\"Person.identifier.orcid\":\"0000-0002-3061-8261\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Pinto, M. Alice Chávez-Galarza, Julio Johnston, J. Spencer Henriques, Dora Rufino, José Muñoz, Irene De la Rúa, Pilar Azevedo, João |
| datacite.date.Accepted.fl_str_mv | 2012-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2013-10-28T10:32:45Z |
| datacite.date.embargoed.fl_str_mv | 2013-10-28T10:32:45Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Abelha Ibérica Apis mellifera iberiensis mtDNA SNPs Landscape genetics Spatial analysis |
| datacite.titles.title.fl_str_mv | Spatial patterns of genetic variation in the Iberian honey bee hybrid zone: a comparison between mitochondrial and nuclear DNA |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Pinto, M. Alice Chávez-Galarza, Julio Johnston, J. Spencer Henriques, Dora Rufino, José Muñoz, Irene De la Rúa, Pilar Azevedo, João |
| dc.date.Accepted.fl_str_mv | 2012-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2013-10-28T10:32:45Z |
| dc.date.embargoed.fl_str_mv | 2013-10-28T10:32:45Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/8926 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Abelha Ibérica Apis mellifera iberiensis mtDNA SNPs Landscape genetics Spatial analysis |
| dc.title.fl_str_mv | Spatial patterns of genetic variation in the Iberian honey bee hybrid zone: a comparison between mitochondrial and nuclear DNA |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6670 |
| description | The Iberian honey bee (Apis mellifera iberiensis) has been intensely surveyed for genetic variation with different markers ranging from morphology, allozymes, mitochondrial DNA (mt DNA), to microsatellites. Some of these markers have revealed non-concordant complex patterns of genetic variation, which led scientists to evoke competing hypotheses for the origin of Iberian honey bees. While complex patterns and underlying historical processes are typical of hybrid zones, the use of more powerful molecular and analytical tools and the fine-scale sampling promised to help dissecting the complexities of the Iberian hybrid zone. In this study, we conducted a genome-wide sampling by genotyping over 384 SNPs (single nucleotide polymorphisms) and sequenced an intergenic fragment of the mt DNA in 711 georeferenced honey bee samples collected across three North-South transects in the Iberian Peninsula. Both mt DNA and SNP datasets were analyzed using spatial tools to represent the structure generated by both types of molecular markers. We found concordant spatial patterns between markers which led to rejection of the standing hypothesis of recent human introductions and selection as the processes shaping Iberian honey bees patterns. This study shows that the fine-scale genomic and spatial analyses can reveal patterns which would otherwise had been undetected. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | conferencePoster |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/518d3b67-7d56-49a6-bf46-ccfda6bd48cd/download |
| funding.funder.alternateName_str_mv | FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia |
| id | ipb_fc8a9d8bcddc2999516f5182ce45d4ea |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/8926 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/8926 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Pinto, M. Alice Pinto, M. Alice https://www.ciencia-id.pt/F814-A1D0-8318 F814-A1D0-8318 http://orcid.org/0000-0001-9663-8399 0000-0001-9663-8399 Chávez-Galarza, Julio Johnston, J. Spencer Henriques, Dora Henriques, Dora https://www.ciencia-id.pt/291F-986F-07DA 291F-986F-07DA http://orcid.org/0000-0001-7530-682X 0000-0001-7530-682X Rufino, José Rufino, José https://www.ciencia-id.pt/C414-F47F-6323 C414-F47F-6323 http://orcid.org/0000-0002-1344-8264 0000-0002-1344-8264 Muñoz, Irene De la Rúa, Pilar Azevedo, João Azevedo, João https://www.ciencia-id.pt/3F1F-0829-5878 3F1F-0829-5878 http://orcid.org/0000-0002-3061-8261 0000-0002-3061-8261 |
| publishDate | 2012 |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engporThe Iberian honey bee (Apis mellifera iberiensis) has been intensely surveyed for genetic variation with different markers ranging from morphology, allozymes, mitochondrial DNA (mt DNA), to microsatellites. Some of these markers have revealed non-concordant complex patterns of genetic variation, which led scientists to evoke competing hypotheses for the origin of Iberian honey bees. While complex patterns and underlying historical processes are typical of hybrid zones, the use of more powerful molecular and analytical tools and the fine-scale sampling promised to help dissecting the complexities of the Iberian hybrid zone. In this study, we conducted a genome-wide sampling by genotyping over 384 SNPs (single nucleotide polymorphisms) and sequenced an intergenic fragment of the mt DNA in 711 georeferenced honey bee samples collected across three North-South transects in the Iberian Peninsula. Both mt DNA and SNP datasets were analyzed using spatial tools to represent the structure generated by both types of molecular markers. We found concordant spatial patterns between markers which led to rejection of the standing hypothesis of recent human introductions and selection as the processes shaping Iberian honey bees patterns. This study shows that the fine-scale genomic and spatial analyses can reveal patterns which would otherwise had been undetected.application/pdfporSpatial patterns of genetic variation in the Iberian honey bee hybrid zone: a comparison between mitochondrial and nuclear DNAPersonalPinto, M. AliceDSpacehttp://dspace.org/items/0667fe04-7078-483d-9198-56d167b19bc5DSpacehttp://dspace.org/items/0667fe04-7078-483d-9198-56d167b19bc5PintoM. AliceCiência IDhttps://www.ciencia-id.ptF814-A1D0-8318ORCIDhttp://orcid.org0000-0001-9663-8399Scopus Author IDhttps://www.scopus.com8085507800Chávez-Galarza, JulioJohnston, J. SpencerPersonalHenriques, DoraDSpacehttp://dspace.org/items/d2abd09f-a90c-4cfb-9a60-7fc32f56184dDSpacehttp://dspace.org/items/d2abd09f-a90c-4cfb-9a60-7fc32f56184dHenriquesDoraCiência IDhttps://www.ciencia-id.pt291F-986F-07DAORCIDhttp://orcid.org0000-0001-7530-682XScopus Author IDhttps://www.scopus.com55761737300PersonalRufino, JoséDSpacehttp://dspace.org/items/1e24d2ce-a354-442a-bef8-eebadd94b385DSpacehttp://dspace.org/items/1e24d2ce-a354-442a-bef8-eebadd94b385RufinoJoséCiência IDhttps://www.ciencia-id.ptC414-F47F-6323ORCIDhttp://orcid.org0000-0002-1344-8264Scopus Author IDhttps://www.scopus.com55947199100Scopus Author IDhttps://www.scopus.com57188967176Muñoz, IreneDe la Rúa, PilarPersonalAzevedo, JoãoDSpacehttp://dspace.org/items/e15b0e75-689e-4be0-942c-1958cfefdb0fDSpacehttp://dspace.org/items/e15b0e75-689e-4be0-942c-1958cfefdb0fAzevedoJoão C.Ciência IDhttps://www.ciencia-id.pt3F1F-0829-5878ORCIDhttp://orcid.org0000-0002-3061-8261HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.pt2013-10-28T10:32:45Z20122012-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/8926http://purl.org/coar/access_right/c_abf2open accessAbelha IbéricaApis mellifera iberiensismtDNASNPsLandscape geneticsSpatial analysis351251 bytesFundação para a Ciência e a TecnologiaPOPULATION GENOMICS AND LANDSCAPE GENETICS OF THE IBERIAN HONEY BEE APIS MELLIFERA IBERIENSISCrossref Funder IDhttp://doi.org/10.13039/501100001871other research producthttp://purl.org/coar/resource_type/c_6670conference posterhttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/518d3b67-7d56-49a6-bf46-ccfda6bd48cd/downloadIUFRO Landscape Ecology Working Group International ConferenceConcepción, Chile |
| spellingShingle | Spatial patterns of genetic variation in the Iberian honey bee hybrid zone: a comparison between mitochondrial and nuclear DNA Pinto, M. Alice Abelha Ibérica Apis mellifera iberiensis mtDNA SNPs Landscape genetics Spatial analysis |
| status | SINGLETON |
| subject.fl_str_mv | Abelha Ibérica Apis mellifera iberiensis mtDNA SNPs Landscape genetics Spatial analysis |
| title | Spatial patterns of genetic variation in the Iberian honey bee hybrid zone: a comparison between mitochondrial and nuclear DNA |
| title_full | Spatial patterns of genetic variation in the Iberian honey bee hybrid zone: a comparison between mitochondrial and nuclear DNA |
| title_fullStr | Spatial patterns of genetic variation in the Iberian honey bee hybrid zone: a comparison between mitochondrial and nuclear DNA |
| title_full_unstemmed | Spatial patterns of genetic variation in the Iberian honey bee hybrid zone: a comparison between mitochondrial and nuclear DNA |
| title_short | Spatial patterns of genetic variation in the Iberian honey bee hybrid zone: a comparison between mitochondrial and nuclear DNA |
| title_sort | Spatial patterns of genetic variation in the Iberian honey bee hybrid zone: a comparison between mitochondrial and nuclear DNA |
| topic | Abelha Ibérica Apis mellifera iberiensis mtDNA SNPs Landscape genetics Spatial analysis |
| topic_facet | Abelha Ibérica Apis mellifera iberiensis mtDNA SNPs Landscape genetics Spatial analysis |
| url | http://hdl.handle.net/10198/8926 |
| visible | 1 |