Publicação
In vitro culture conditions of colletotrichum gloeosporioides: b) Effect of temperature shock on spore production
| Resumo: | Hypericum perfuratum is an important plant species used for the production of hypericin, a metabolite of major application in the pharmaceutical industry. Its production is, however, strongly limited by Colletotrichum gloeosporioides, the causative agent of anthracnose. As H. perforatum tolerance to C. gloeosporioides is dependent on plant variety, we intend to select tolerant varieties under in vitro conditions for metabolite production without fungicide application. Selective pressure on plants is induced by inoculation with the fungus spores, but spores are difficult to obtain. The establishment of in vitro conditions for spore production was the main goal of the trials here presented. The influence of changing temperature on spore production of C. gloeosporioides was tested. Four different temperature conditions were tested, using the environment temperature as reference. We tested the influence of an initial cold and heat shock, as well as a cold and heat shock after growth under environment conditions. Preliminary results showed that an initial heat shock induces spore production in C. gloeosporioides. Complementary trials on spore production and in vitro plant inoculation are being developed. |
|---|---|
| Autores principais: | Offerman, J. |
| Outros Autores: | Sousa, Maria João; Rodrigues, Paula; Martins, Anabela; Dias, Alberto |
| Assunto: | Colletotrichum Spore production |
| Ano: | 2005 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867172927053496320 |
|---|---|
| author | Offerman, J. |
| author2 | Sousa, Maria João Rodrigues, Paula Martins, Anabela Dias, Alberto |
| author2_role | author author author author |
| author_facet | Offerman, J. Sousa, Maria João Rodrigues, Paula Martins, Anabela Dias, Alberto |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Offerman, J.\"},{\"Person.name\":\"Sousa, Maria João\",\"Person.identifier.orcid\":\"0000-0002-9946-4926\"},{\"Person.name\":\"Rodrigues, Paula\",\"Person.identifier.orcid\":\"0000-0002-3789-2730\"},{\"Person.name\":\"Martins, Anabela\",\"Person.identifier.orcid\":\"0000-0001-6218-4413\"},{\"Person.name\":\"Dias, Alberto\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Offerman, J. Sousa, Maria João Rodrigues, Paula Martins, Anabela Dias, Alberto |
| datacite.date.Accepted.fl_str_mv | 2005-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2008-12-22T11:10:29Z |
| datacite.date.embargoed.fl_str_mv | 2008-12-22T11:10:29Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Colletotrichum Spore production |
| datacite.titles.title.fl_str_mv | In vitro culture conditions of colletotrichum gloeosporioides: b) Effect of temperature shock on spore production |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Offerman, J. Sousa, Maria João Rodrigues, Paula Martins, Anabela Dias, Alberto |
| dc.date.Accepted.fl_str_mv | 2005-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2008-12-22T11:10:29Z |
| dc.date.embargoed.fl_str_mv | 2008-12-22T11:10:29Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/952 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | The British Society for Plant Pathology |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Colletotrichum Spore production |
| dc.title.fl_str_mv | In vitro culture conditions of colletotrichum gloeosporioides: b) Effect of temperature shock on spore production |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Hypericum perfuratum is an important plant species used for the production of hypericin, a metabolite of major application in the pharmaceutical industry. Its production is, however, strongly limited by Colletotrichum gloeosporioides, the causative agent of anthracnose. As H. perforatum tolerance to C. gloeosporioides is dependent on plant variety, we intend to select tolerant varieties under in vitro conditions for metabolite production without fungicide application. Selective pressure on plants is induced by inoculation with the fungus spores, but spores are difficult to obtain. The establishment of in vitro conditions for spore production was the main goal of the trials here presented. The influence of changing temperature on spore production of C. gloeosporioides was tested. Four different temperature conditions were tested, using the environment temperature as reference. We tested the influence of an initial cold and heat shock, as well as a cold and heat shock after growth under environment conditions. Preliminary results showed that an initial heat shock induces spore production in C. gloeosporioides. Complementary trials on spore production and in vitro plant inoculation are being developed. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | conferenceObject |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/19fafc5a-a49a-4439-9934-5dea37c756a8/download |
| funding.funder.alternateName_str_mv | FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | POCI |
| id | ipb_fd1501e5e7e283f8da219e15e5b910c0 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/952 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/952 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Offerman, J. Sousa, Maria João Sousa, Maria João https://www.ciencia-id.pt/D91E-3571-3979 D91E-3571-3979 http://orcid.org/0000-0002-9946-4926 0000-0002-9946-4926 Rodrigues, Paula Rodrigues, Paula https://www.ciencia-id.pt/2312-18AE-DA42 2312-18AE-DA42 http://orcid.org/0000-0002-3789-2730 0000-0002-3789-2730 Martins, Anabela Martins, Anabela https://www.ciencia-id.pt/7B18-A810-6B93 7B18-A810-6B93 http://orcid.org/0000-0001-6218-4413 0000-0001-6218-4413 Dias, Alberto |
| publishDate | 2005 |
| publisher.none.fl_str_mv | The British Society for Plant Pathology |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engThe British Society for Plant PathologyenHypericum perfuratum is an important plant species used for the production of hypericin, a metabolite of major application in the pharmaceutical industry. Its production is, however, strongly limited by Colletotrichum gloeosporioides, the causative agent of anthracnose. As H. perforatum tolerance to C. gloeosporioides is dependent on plant variety, we intend to select tolerant varieties under in vitro conditions for metabolite production without fungicide application. Selective pressure on plants is induced by inoculation with the fungus spores, but spores are difficult to obtain. The establishment of in vitro conditions for spore production was the main goal of the trials here presented. The influence of changing temperature on spore production of C. gloeosporioides was tested. Four different temperature conditions were tested, using the environment temperature as reference. We tested the influence of an initial cold and heat shock, as well as a cold and heat shock after growth under environment conditions. Preliminary results showed that an initial heat shock induces spore production in C. gloeosporioides. Complementary trials on spore production and in vitro plant inoculation are being developed.application/pdfenIn vitro culture conditions of colletotrichum gloeosporioides: b) Effect of temperature shock on spore productionOfferman, J.PersonalSousa, Maria JoãoDSpacehttp://dspace.org/items/da5c7615-9844-4fdf-b4d4-64d0c8e72de0DSpacehttp://dspace.org/items/da5c7615-9844-4fdf-b4d4-64d0c8e72de0SousaMaria JoãoCiência IDhttps://www.ciencia-id.ptD91E-3571-3979ORCIDhttp://orcid.org0000-0002-9946-4926Scopus Author IDhttps://www.scopus.com55230282700PersonalRodrigues, PaulaDSpacehttp://dspace.org/items/b864b8c1-d66a-419c-b1ac-e6193f93eca6DSpacehttp://dspace.org/items/b864b8c1-d66a-419c-b1ac-e6193f93eca6RodriguesPaulaCiência IDhttps://www.ciencia-id.pt2312-18AE-DA42ORCIDhttp://orcid.org0000-0002-3789-2730Scopus Author IDhttps://www.scopus.com7102639646PersonalMartins, AnabelaDSpacehttp://dspace.org/items/383370dd-10e5-40e6-9a15-f2201fe95581DSpacehttp://dspace.org/items/383370dd-10e5-40e6-9a15-f2201fe95581MartinsAnabelaCiência IDhttps://www.ciencia-id.pt7B18-A810-6B93ORCIDhttp://orcid.org0000-0001-6218-4413Researcher IDhttps://www.researcherid.comG-5488-2013Scopus Author IDhttps://www.scopus.com7203013518Dias, AlbertoHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.pt2008-12-22T11:10:29Z20052005-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/952http://purl.org/coar/access_right/c_abf2open accessColletotrichumSpore production1852391 bytesFundação para a Ciência e a TecnologiaStudy of defense mechanisms of Hypericum perforatum against Colletotrichum gloeosporioides infection anthracnosePOCICrossref Funder IDhttp://doi.org/10.13039/501100001871other research producthttp://purl.org/coar/resource_type/c_c94fconference objecthttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/19fafc5a-a49a-4439-9934-5dea37c756a8/download |
| spellingShingle | In vitro culture conditions of colletotrichum gloeosporioides: b) Effect of temperature shock on spore production Offerman, J. Colletotrichum Spore production |
| status | SINGLETON |
| subject.fl_str_mv | Colletotrichum Spore production |
| title | In vitro culture conditions of colletotrichum gloeosporioides: b) Effect of temperature shock on spore production |
| title_full | In vitro culture conditions of colletotrichum gloeosporioides: b) Effect of temperature shock on spore production |
| title_fullStr | In vitro culture conditions of colletotrichum gloeosporioides: b) Effect of temperature shock on spore production |
| title_full_unstemmed | In vitro culture conditions of colletotrichum gloeosporioides: b) Effect of temperature shock on spore production |
| title_short | In vitro culture conditions of colletotrichum gloeosporioides: b) Effect of temperature shock on spore production |
| title_sort | In vitro culture conditions of colletotrichum gloeosporioides: b) Effect of temperature shock on spore production |
| topic | Colletotrichum Spore production |
| topic_facet | Colletotrichum Spore production |
| url | http://hdl.handle.net/10198/952 |
| visible | 1 |