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Development of a natural ingredient from kiwi epicarp with potential application in the food industry

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Resumo:Kiwifruit is a recognized source of bioactive compounds with beneficial properties for consumers’ health [1]. Although only pulp is normally consumed, there are several studies in literature that have been exploring the fruits epicarp, which is characterized as an excellent source of bioactive compounds. Thus, since the food industry produces annually tons of this normally discarded and unused residue [2], this work aimed to characterize two varieties of kiwi (Actinidia delicious cv. "Hayward" - green pulp kiwi variety and Actinidia spp. - red pulp kiwi variety), comparing the two pulps and epicarps. This study will valorize these bio-residues. The proximal composition (protein, fat, carbohydrates and ash contents, and energy) of the pulp of these varieties, as well as the composition in free sugars, fatty acids, organic acids and tocopherols, and the study of the bioactivities (antioxidant, anti-inflammatory, cytotoxic and antimicrobial activities) of the pulp and epicarp were evaluated. A hydroethanolic extraction was used to obtain compounds subsequently identified and quantified by HPLC-DAD-ESI/MS. The results revealed that pulps have a high water content and low amount of other macronutrients. Both epicarp varieties presented a higher concentration of phenolic compounds than the corresponding pulp. Additionally, only Actinidia spp. (red pulp kiwi variety) presented an anthocyanin, cyanidin-O-pentosylhexoside. α-tocopherol was the most abundant vitamer in all the analyzed parts. Four organic acids (quinic, malic, citric and ascorbic acids) were identified in all the studied samples. The best antioxidant activity was shown by the epicarps, highlighting the green pulp kiwi as the best variety. Similarly, only the epicarp of the green pulpkiwi variety presented positive results for all the tested cell lines, being able to inhibit the cell growth in a moderate way. Additionally, none of the extracts showed toxicity in a porcine liver cell line at the highest tested concentration (GI50> 400 μg/mL). Both kiwi varieties also demonstrated an excellent antimicrobial activity against some pathogenic bacteria. These results confirm the potential application of kiwi epicarp in the food industry as a source of functional compounds with bioactive properties, contributing in the valorization of these bio-residues.
Autores principais:Caleja, Cristina
Outros Autores:Dias, Maria Inês; Pereira, Carla; Calhelha, Ricardo C.; Kostić, Marina; Soković, Marina; Tavares, Débora; Baraldi, Ilton J.; Barros, Lillian; Ferreira, Isabel C.F.R.
Assunto:Kiwi Bioactive compounds
Ano:2019
País:Portugal
Tipo de documento:documento de conferência
Tipo de acesso:acesso aberto
Instituição associada:Instituto Politécnico de Bragança
Idioma:inglês
Origem:Biblioteca Digital do IPB
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author Caleja, Cristina
author2 Dias, Maria Inês
Pereira, Carla
Calhelha, Ricardo C.
Kostić, Marina
Soković, Marina
Tavares, Débora
Baraldi, Ilton J.
Barros, Lillian
Ferreira, Isabel C.F.R.
author2_role author
author
author
author
author
author
author
author
author
author_facet Caleja, Cristina
Dias, Maria Inês
Pereira, Carla
Calhelha, Ricardo C.
Kostić, Marina
Soković, Marina
Tavares, Débora
Baraldi, Ilton J.
Barros, Lillian
Ferreira, Isabel C.F.R.
author_role author
contributor_name_str_mv Biblioteca Digital do IPB
country_str PT
creators_json_txt [{\"Person.name\":\"Caleja, Cristina\",\"Person.identifier.orcid\":\"0000-0002-5769-8484\"},{\"Person.name\":\"Dias, Maria Inês\",\"Person.identifier.orcid\":\"0000-0001-8744-7814\"},{\"Person.name\":\"Pereira, Carla\",\"Person.identifier.orcid\":\"0000-0003-0093-771X\"},{\"Person.name\":\"Calhelha, Ricardo C.\",\"Person.identifier.orcid\":\"0000-0002-6801-4578\"},{\"Person.name\":\"Kostić, Marina\"},{\"Person.name\":\"Soković, Marina\"},{\"Person.name\":\"Tavares, Débora\"},{\"Person.name\":\"Baraldi, Ilton J.\"},{\"Person.name\":\"Barros, Lillian\",\"Person.identifier.orcid\":\"0000-0002-9050-5189\"},{\"Person.name\":\"Ferreira, Isabel C.F.R.\",\"Person.identifier.orcid\":\"0000-0003-4910-4882\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Biblioteca Digital do IPB
datacite.creators.creator.creatorName.fl_str_mv Caleja, Cristina
Dias, Maria Inês
Pereira, Carla
Calhelha, Ricardo C.
Kostić, Marina
Soković, Marina
Tavares, Débora
Baraldi, Ilton J.
Barros, Lillian
Ferreira, Isabel C.F.R.
datacite.date.Accepted.fl_str_mv 2019-01-01T00:00:00Z
datacite.date.available.fl_str_mv 2022-01-27T09:57:40Z
datacite.date.embargoed.fl_str_mv 2022-01-27T09:57:40Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.subjects.subject.fl_str_mv Kiwi
Bioactive compounds
datacite.titles.title.fl_str_mv Development of a natural ingredient from kiwi epicarp with potential application in the food industry
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.creator.none.fl_str_mv Caleja, Cristina
Dias, Maria Inês
Pereira, Carla
Calhelha, Ricardo C.
Kostić, Marina
Soković, Marina
Tavares, Débora
Baraldi, Ilton J.
Barros, Lillian
Ferreira, Isabel C.F.R.
dc.date.Accepted.fl_str_mv 2019-01-01T00:00:00Z
dc.date.available.fl_str_mv 2022-01-27T09:57:40Z
dc.date.embargoed.fl_str_mv 2022-01-27T09:57:40Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://hdl.handle.net/10198/24932
dc.language.none.fl_str_mv eng
dc.rights.cclincense.fl_str_mv http://creativecommons.org/licenses/by/4.0/
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.subject.none.fl_str_mv Kiwi
Bioactive compounds
dc.title.fl_str_mv Development of a natural ingredient from kiwi epicarp with potential application in the food industry
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_c94f
description Kiwifruit is a recognized source of bioactive compounds with beneficial properties for consumers’ health [1]. Although only pulp is normally consumed, there are several studies in literature that have been exploring the fruits epicarp, which is characterized as an excellent source of bioactive compounds. Thus, since the food industry produces annually tons of this normally discarded and unused residue [2], this work aimed to characterize two varieties of kiwi (Actinidia delicious cv. "Hayward" - green pulp kiwi variety and Actinidia spp. - red pulp kiwi variety), comparing the two pulps and epicarps. This study will valorize these bio-residues. The proximal composition (protein, fat, carbohydrates and ash contents, and energy) of the pulp of these varieties, as well as the composition in free sugars, fatty acids, organic acids and tocopherols, and the study of the bioactivities (antioxidant, anti-inflammatory, cytotoxic and antimicrobial activities) of the pulp and epicarp were evaluated. A hydroethanolic extraction was used to obtain compounds subsequently identified and quantified by HPLC-DAD-ESI/MS. The results revealed that pulps have a high water content and low amount of other macronutrients. Both epicarp varieties presented a higher concentration of phenolic compounds than the corresponding pulp. Additionally, only Actinidia spp. (red pulp kiwi variety) presented an anthocyanin, cyanidin-O-pentosylhexoside. α-tocopherol was the most abundant vitamer in all the analyzed parts. Four organic acids (quinic, malic, citric and ascorbic acids) were identified in all the studied samples. The best antioxidant activity was shown by the epicarps, highlighting the green pulp kiwi as the best variety. Similarly, only the epicarp of the green pulpkiwi variety presented positive results for all the tested cell lines, being able to inhibit the cell growth in a moderate way. Additionally, none of the extracts showed toxicity in a porcine liver cell line at the highest tested concentration (GI50> 400 μg/mL). Both kiwi varieties also demonstrated an excellent antimicrobial activity against some pathogenic bacteria. These results confirm the potential application of kiwi epicarp in the food industry as a source of functional compounds with bioactive properties, contributing in the valorization of these bio-residues.
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funding.funder.alternateName_str_mv FCT
funding.funder.identifier_str_mv http://doi.org/10.13039/501100001871
funding.funder.name_str_mv Fundação para a Ciência e a Tecnologia
funding.name_str_mv 6817 - DCRRNI ID
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person_str_mv Caleja, Cristina
Caleja, Cristina
https://www.ciencia-id.pt/E218-8DD3-4959
E218-8DD3-4959
http://orcid.org/0000-0002-5769-8484
0000-0002-5769-8484
Dias, Maria Inês
Dias, Maria Inês
https://www.ciencia-id.pt/2A13-4BE6-C7CF
2A13-4BE6-C7CF
http://orcid.org/0000-0001-8744-7814
0000-0001-8744-7814
Pereira, Carla
Pereira, Carla
https://www.ciencia-id.pt/EF10-2739-2B70
EF10-2739-2B70
http://orcid.org/0000-0003-0093-771X
0000-0003-0093-771X
Calhelha, Ricardo C.
Calhelha, Ricardo C.
https://www.ciencia-id.pt/F313-E3CE-554E
F313-E3CE-554E
http://orcid.org/0000-0002-6801-4578
0000-0002-6801-4578
Kostić, Marina
Soković, Marina
Tavares, Débora
Baraldi, Ilton J.
Barros, Lillian
Barros, Lillian
https://www.ciencia-id.pt/9616-35CB-D001
9616-35CB-D001
http://orcid.org/0000-0002-9050-5189
0000-0002-9050-5189
Ferreira, Isabel C.F.R.
Ferreira, Isabel C.F.R.
https://www.ciencia-id.pt/9418-CF95-9919
9418-CF95-9919
http://orcid.org/0000-0003-4910-4882
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publishDate 2019
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spelling engpt_PTKiwifruit is a recognized source of bioactive compounds with beneficial properties for consumers’ health [1]. Although only pulp is normally consumed, there are several studies in literature that have been exploring the fruits epicarp, which is characterized as an excellent source of bioactive compounds. Thus, since the food industry produces annually tons of this normally discarded and unused residue [2], this work aimed to characterize two varieties of kiwi (Actinidia delicious cv. "Hayward" - green pulp kiwi variety and Actinidia spp. - red pulp kiwi variety), comparing the two pulps and epicarps. This study will valorize these bio-residues. The proximal composition (protein, fat, carbohydrates and ash contents, and energy) of the pulp of these varieties, as well as the composition in free sugars, fatty acids, organic acids and tocopherols, and the study of the bioactivities (antioxidant, anti-inflammatory, cytotoxic and antimicrobial activities) of the pulp and epicarp were evaluated. A hydroethanolic extraction was used to obtain compounds subsequently identified and quantified by HPLC-DAD-ESI/MS. The results revealed that pulps have a high water content and low amount of other macronutrients. Both epicarp varieties presented a higher concentration of phenolic compounds than the corresponding pulp. Additionally, only Actinidia spp. (red pulp kiwi variety) presented an anthocyanin, cyanidin-O-pentosylhexoside. α-tocopherol was the most abundant vitamer in all the analyzed parts. Four organic acids (quinic, malic, citric and ascorbic acids) were identified in all the studied samples. The best antioxidant activity was shown by the epicarps, highlighting the green pulp kiwi as the best variety. Similarly, only the epicarp of the green pulpkiwi variety presented positive results for all the tested cell lines, being able to inhibit the cell growth in a moderate way. Additionally, none of the extracts showed toxicity in a porcine liver cell line at the highest tested concentration (GI50> 400 μg/mL). Both kiwi varieties also demonstrated an excellent antimicrobial activity against some pathogenic bacteria. These results confirm the potential application of kiwi epicarp in the food industry as a source of functional compounds with bioactive properties, contributing in the valorization of these bio-residues.application/pdfpt_PTDevelopment of a natural ingredient from kiwi epicarp with potential application in the food industryPersonalCaleja, CristinaDSpacehttp://dspace.org/items/5ef2706d-073c-4c23-9e45-498ac244118cDSpacehttp://dspace.org/items/5ef2706d-073c-4c23-9e45-498ac244118cCalejaCristinaCiência IDhttps://www.ciencia-id.ptE218-8DD3-4959ORCIDhttp://orcid.org0000-0002-5769-8484Scopus Author IDhttps://www.scopus.com36514202500PersonalDias, Maria InêsDSpacehttp://dspace.org/items/7ca8432b-e7f4-4116-a56e-9eba0d4403bdDSpacehttp://dspace.org/items/7ca8432b-e7f4-4116-a56e-9eba0d4403bdDiasMaria InêsCiência IDhttps://www.ciencia-id.pt2A13-4BE6-C7CFORCIDhttp://orcid.org0000-0001-8744-7814Researcher IDhttps://www.researcherid.comM-8242-2013Scopus Author IDhttps://www.scopus.com54388787000PersonalPereira, CarlaDSpacehttp://dspace.org/items/e07a8e12-52bb-47c5-bae7-41a5f648213bDSpacehttp://dspace.org/items/e07a8e12-52bb-47c5-bae7-41a5f648213bPereiraCarlaCiência IDhttps://www.ciencia-id.ptEF10-2739-2B70ORCIDhttp://orcid.org0000-0003-0093-771XResearcher IDhttps://www.researcherid.comK-1629-2016Scopus Author IDhttps://www.scopus.com55627876424PersonalCalhelha, Ricardo C.DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053CalhelhaRicardo C.Ciência IDhttps://www.ciencia-id.ptF313-E3CE-554EORCIDhttp://orcid.org0000-0002-6801-4578Researcher IDhttps://www.researcherid.comJ-2172-2014Scopus Author IDhttps://www.scopus.com6507978333Kostić, MarinaSoković, MarinaTavares, DéboraBaraldi, Ilton J.PersonalBarros, LillianDSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01DSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01BarrosLillianCiência IDhttps://www.ciencia-id.pt9616-35CB-D001ORCIDhttp://orcid.org0000-0002-9050-5189Researcher IDhttps://www.researcherid.comJ-3600-2013Researcher IDhttps://www.researcherid.comJ-3600-2013Scopus Author IDhttps://www.scopus.com35236343600Scopus Author IDhttps://www.scopus.com57194900801PersonalFerreira, Isabel C.F.R.DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8FerreiraIsabel C.F.R.Ciência IDhttps://www.ciencia-id.pt9418-CF95-9919ORCIDhttp://orcid.org0000-0003-4910-4882Researcher IDhttps://www.researcherid.comE-8500-2013Scopus Author IDhttps://www.scopus.com36868826600HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISBNIsPartOf978-84-09-16320-52022-01-27T09:57:40Z20192019-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/24932http://purl.org/coar/access_right/c_abf2open accessKiwiBioactive compounds936603 bytesFundação para a Ciência e a TecnologiaMountain Research Centre6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871other research producthttp://purl.org/coar/resource_type/c_c94fconference object2019http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/4c0ef87f-7d4c-4c1a-b4c2-232894d5a051/downloadXXV Encontro Galego-Portugués de QuímicaSantiago de Compostela, Espanhã
spellingShingle Development of a natural ingredient from kiwi epicarp with potential application in the food industry
Caleja, Cristina
Kiwi
Bioactive compounds
status SINGLETON
subject.fl_str_mv Kiwi
Bioactive compounds
title Development of a natural ingredient from kiwi epicarp with potential application in the food industry
title_full Development of a natural ingredient from kiwi epicarp with potential application in the food industry
title_fullStr Development of a natural ingredient from kiwi epicarp with potential application in the food industry
title_full_unstemmed Development of a natural ingredient from kiwi epicarp with potential application in the food industry
title_short Development of a natural ingredient from kiwi epicarp with potential application in the food industry
title_sort Development of a natural ingredient from kiwi epicarp with potential application in the food industry
topic Kiwi
Bioactive compounds
topic_facet Kiwi
Bioactive compounds
url http://hdl.handle.net/10198/24932
visible 1