Publicação
Bridging sd1 molecular knowledge with recent breeding strategies for the improvement of traditional rice varieties - a japonica case-study
| Resumo: | The rice semidwarfing gene, sd1, also known as the “green revolution gene”, has been studied intensively due to its contribution to the increase of crop production. Although sd1 breeding was extensively applied since the 1960s, the recent advances in the molecular basis of this gene allowed designing a more precise breeding strategy - marker assisted backcrossing (MAB) - to track sd1 introgression in two traditional rice varieties. For selection of sd1 plants we first confirmed the efficiency of specific markers based on Os200 x 2 gene sequence. Background selection was also performed with the help of microsatellites markers (SSR) and a total of 7 breeding lines were recovered containing a higher percentage of recurrent parent genome (RPG). Analysis of Covariance (ANCOVA) using mean progenitor plant height as covariate was performed to compare several agronomic and quality-related parameters in two different environments. The results suggest that plant height differs significantly between the two environments F(1, 220) = 155.336; p < 0.001. From the total variability of plant height we could conclude that 73% is due to the genotype, while 10.4% depends on the environment. In addition, the percentage of RPG seems negatively correlated with plant height (p < 0.005). MAB and background selection thus revealed as useful tools to assist breeding for semidwarfism in traditional rice varieties. |
|---|---|
| Autores principais: | Negrão, Sónia |
| Outros Autores: | Palaniappan, Jayamani; Maroco, João; Lourenço, Tiago; Mackill, David; Oliveira, Maria Margarida |
| Assunto: | Rice Semidwarfism Sd1 Environmental influence |
| Ano: | 2010 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Ispa-Instituto Universitário |
| Idioma: | inglês |
| Origem: | Repositório do Ispa - Instituto Universitário |
| _version_ | 1868077193005891584 |
|---|---|
| author | Negrão, Sónia |
| author2 | Palaniappan, Jayamani Maroco, João Lourenço, Tiago Mackill, David Oliveira, Maria Margarida |
| author2_role | author author author author author |
| author_facet | Negrão, Sónia Palaniappan, Jayamani Maroco, João Lourenço, Tiago Mackill, David Oliveira, Maria Margarida |
| author_role | author |
| contributor_name_str_mv | Repositório do ISPA |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Negrão, Sónia\"},{\"Person.name\":\"Palaniappan, Jayamani\"},{\"Person.name\":\"Maroco, João\"},{\"Person.name\":\"Lourenço, Tiago\"},{\"Person.name\":\"Mackill, David\"},{\"Person.name\":\"Oliveira, Maria Margarida\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório do ISPA |
| datacite.creators.creator.creatorName.fl_str_mv | Negrão, Sónia Palaniappan, Jayamani Maroco, João Lourenço, Tiago Mackill, David Oliveira, Maria Margarida |
| datacite.date.Accepted.fl_str_mv | 2010-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2012-09-11T18:07:25Z |
| datacite.date.embargoed.fl_str_mv | 2012-09-11T18:07:25Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Rice Semidwarfism Sd1 Environmental influence |
| datacite.titles.title.fl_str_mv | Bridging sd1 molecular knowledge with recent breeding strategies for the improvement of traditional rice varieties - a japonica case-study |
| dc.contributor.none.fl_str_mv | Repositório do ISPA |
| dc.creator.none.fl_str_mv | Negrão, Sónia Palaniappan, Jayamani Maroco, João Lourenço, Tiago Mackill, David Oliveira, Maria Margarida |
| dc.date.Accepted.fl_str_mv | 2010-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2012-09-11T18:07:25Z |
| dc.date.embargoed.fl_str_mv | 2012-09-11T18:07:25Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.12/1688 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Academic Journals |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Rice Semidwarfism Sd1 Environmental influence |
| dc.title.fl_str_mv | Bridging sd1 molecular knowledge with recent breeding strategies for the improvement of traditional rice varieties - a japonica case-study |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The rice semidwarfing gene, sd1, also known as the “green revolution gene”, has been studied intensively due to its contribution to the increase of crop production. Although sd1 breeding was extensively applied since the 1960s, the recent advances in the molecular basis of this gene allowed designing a more precise breeding strategy - marker assisted backcrossing (MAB) - to track sd1 introgression in two traditional rice varieties. For selection of sd1 plants we first confirmed the efficiency of specific markers based on Os200 x 2 gene sequence. Background selection was also performed with the help of microsatellites markers (SSR) and a total of 7 breeding lines were recovered containing a higher percentage of recurrent parent genome (RPG). Analysis of Covariance (ANCOVA) using mean progenitor plant height as covariate was performed to compare several agronomic and quality-related parameters in two different environments. The results suggest that plant height differs significantly between the two environments F(1, 220) = 155.336; p < 0.001. From the total variability of plant height we could conclude that 73% is due to the genotype, while 10.4% depends on the environment. In addition, the percentage of RPG seems negatively correlated with plant height (p < 0.005). MAB and background selection thus revealed as useful tools to assist breeding for semidwarfism in traditional rice varieties. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ispa.pt/bitstreams/1a886e15-9407-42b3-8a48-d1ea088e1ce9/download |
| id | ispa_a1f193bf300a3fb0b6efea2f8fa425d7 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.12/1688 |
| instacron_str | ispa |
| institution | Ispa-Instituto Universitário |
| instname_str | Ispa-Instituto Universitário |
| language | eng |
| network_acronym_str | ispa |
| network_name_str | Repositório do Ispa - Instituto Universitário |
| oai_identifier_str | oai:repositorio.ispa.pt:10400.12/1688 |
| organization_str_mv | urn:organizationAcronym:ispa |
| person_str_mv | Negrão, Sónia Palaniappan, Jayamani Maroco, João Lourenço, Tiago Mackill, David Oliveira, Maria Margarida |
| publishDate | 2010 |
| publisher.none.fl_str_mv | Academic Journals |
| reponame_str | Repositório do Ispa - Instituto Universitário |
| repository_id_str | urn:repositoryAcronym:ispa |
| service_str_mv | urn:repositoryAcronym:ispa |
| spelling | engAcademic JournalsporThe rice semidwarfing gene, sd1, also known as the “green revolution gene”, has been studied intensively due to its contribution to the increase of crop production. Although sd1 breeding was extensively applied since the 1960s, the recent advances in the molecular basis of this gene allowed designing a more precise breeding strategy - marker assisted backcrossing (MAB) - to track sd1 introgression in two traditional rice varieties. For selection of sd1 plants we first confirmed the efficiency of specific markers based on Os200 x 2 gene sequence. Background selection was also performed with the help of microsatellites markers (SSR) and a total of 7 breeding lines were recovered containing a higher percentage of recurrent parent genome (RPG). Analysis of Covariance (ANCOVA) using mean progenitor plant height as covariate was performed to compare several agronomic and quality-related parameters in two different environments. The results suggest that plant height differs significantly between the two environments F(1, 220) = 155.336; p < 0.001. From the total variability of plant height we could conclude that 73% is due to the genotype, while 10.4% depends on the environment. In addition, the percentage of RPG seems negatively correlated with plant height (p < 0.005). MAB and background selection thus revealed as useful tools to assist breeding for semidwarfism in traditional rice varieties.application/pdfporBridging sd1 molecular knowledge with recent breeding strategies for the improvement of traditional rice varieties - a japonica case-studyNegrão, SóniaPalaniappan, JayamaniMaroco, JoãoLourenço, TiagoMackill, DavidOliveira, Maria MargaridaHostingInstitutionOrganizationalRepositório do ISPAe-mailmailto:repositorio@ispa.ptrepositorio@ispa.ptISSNIsPartOf1684–53152012-09-11T18:07:25Z20102010-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.12/1688http://purl.org/coar/access_right/c_abf2open accessRiceSemidwarfismSd1Environmental influence371089 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ispa.pt/bitstreams/1a886e15-9407-42b3-8a48-d1ea088e1ce9/downloadAfrican Journal of Biotechnology921922200 |
| spellingShingle | Bridging sd1 molecular knowledge with recent breeding strategies for the improvement of traditional rice varieties - a japonica case-study Negrão, Sónia Rice Semidwarfism Sd1 Environmental influence |
| status | SINGLETON |
| subject.fl_str_mv | Rice Semidwarfism Sd1 Environmental influence |
| title | Bridging sd1 molecular knowledge with recent breeding strategies for the improvement of traditional rice varieties - a japonica case-study |
| title_full | Bridging sd1 molecular knowledge with recent breeding strategies for the improvement of traditional rice varieties - a japonica case-study |
| title_fullStr | Bridging sd1 molecular knowledge with recent breeding strategies for the improvement of traditional rice varieties - a japonica case-study |
| title_full_unstemmed | Bridging sd1 molecular knowledge with recent breeding strategies for the improvement of traditional rice varieties - a japonica case-study |
| title_short | Bridging sd1 molecular knowledge with recent breeding strategies for the improvement of traditional rice varieties - a japonica case-study |
| title_sort | Bridging sd1 molecular knowledge with recent breeding strategies for the improvement of traditional rice varieties - a japonica case-study |
| topic | Rice Semidwarfism Sd1 Environmental influence |
| topic_facet | Rice Semidwarfism Sd1 Environmental influence |
| url | http://hdl.handle.net/10400.12/1688 |
| visible | 1 |