Publicação
Temporal variation in brain transcriptome is associated with the expression of female mimicry as a sequential male alternative reproductive tactic in fish
| Resumo: | Distinct patterns of gene expression often underlie intra- and intersexual differences, and the study of this set of coregulated genes is essential to understand the emergence of complex behavioural phenotypes. Here, we describe the development of a de novo transcriptome and brain gene expression profiles of wild-caught peacock blenny, Salaria pavo, an intertidal fish with sex-role reversal in courtship behaviour (i.e., females are the courting sex) and sequential alternative reproductive tactics in males (i.e., larger and older nest-holder males and smaller and younger sneaker males occur). Sneakers mimic both female's courtship behaviour and nuptial coloration to get access to nests and sneak fertilizations, and later in life transition into nest-holder males. Thus, this species offers the unique opportunity to study how the regulation of gene expression can contribute to intersex phenotypes and to the sequential expression of male and female behavioural phenotypes by the same individual. We found that at the whole brain level, expression of the sneaker tactic was paralleled by broader and divergent gene expression when compared to either females or nest-holder males, which were more similar between themselves. When looking at sex-biased transcripts, sneaker males are intersex rather than being either nest-holder or female-like, and their transcriptome is simultaneously demasculinized for nest-holder-biased transcripts and feminized for female-biased transcripts. These results indicate that evolutionary changes in reproductive plasticity can be achieved through regulation of gene expression, and in particular by varying the magnitude of expression of sex-biased genes, throughout the lifetime of the same individual. |
|---|---|
| Autores principais: | Cardoso, Sara |
| Outros Autores: | Gonçalves, David; Goesmann, Alexander; Canário, Adelino V. M.; Oliveira, Rui Filipe |
| Assunto: | Alternative reproductive tactics Behavioural plasticity Neurogenomic state RNA-seq Salaria Pavo Sex-biased gene expression |
| Ano: | 2018 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Ispa-Instituto Universitário |
| Idioma: | inglês |
| Origem: | Repositório do Ispa - Instituto Universitário |
| _version_ | 1866886898728828928 |
|---|---|
| author | Cardoso, Sara |
| author2 | Gonçalves, David Goesmann, Alexander Canário, Adelino V. M. Oliveira, Rui Filipe |
| author2_role | author author author author |
| author_facet | Cardoso, Sara Gonçalves, David Goesmann, Alexander Canário, Adelino V. M. Oliveira, Rui Filipe |
| author_role | author |
| contributor_name_str_mv | Repositório do ISPA |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Cardoso, Sara\",\"Person.identifier.orcid\":\"0000-0002-1006-2323\"},{\"Person.name\":\"Gonçalves, David\"},{\"Person.name\":\"Goesmann, Alexander\",\"Person.identifier.orcid\":\"0000-0002-7086-2568\"},{\"Person.name\":\"Canário, Adelino V. M.\"},{\"Person.name\":\"Oliveira, Rui Filipe\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório do ISPA |
| datacite.creators.creator.creatorName.fl_str_mv | Cardoso, Sara Gonçalves, David Goesmann, Alexander Canário, Adelino V. M. Oliveira, Rui Filipe |
| datacite.date.Accepted.fl_str_mv | 2018-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2019-04-08T17:38:07Z |
| datacite.date.embargoed.fl_str_mv | 2019-04-08T17:38:07Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Alternative reproductive tactics Behavioural plasticity Neurogenomic state RNA-seq Salaria Pavo Sex-biased gene expression |
| datacite.titles.title.fl_str_mv | Temporal variation in brain transcriptome is associated with the expression of female mimicry as a sequential male alternative reproductive tactic in fish |
| dc.contributor.none.fl_str_mv | Repositório do ISPA |
| dc.creator.none.fl_str_mv | Cardoso, Sara Gonçalves, David Goesmann, Alexander Canário, Adelino V. M. Oliveira, Rui Filipe |
| dc.date.Accepted.fl_str_mv | 2018-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2019-04-08T17:38:07Z |
| dc.date.embargoed.fl_str_mv | 2019-04-08T17:38:07Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.12/6986 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Blackwell Publishing Inc. |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by-nc/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Alternative reproductive tactics Behavioural plasticity Neurogenomic state RNA-seq Salaria Pavo Sex-biased gene expression |
| dc.title.fl_str_mv | Temporal variation in brain transcriptome is associated with the expression of female mimicry as a sequential male alternative reproductive tactic in fish |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Distinct patterns of gene expression often underlie intra- and intersexual differences, and the study of this set of coregulated genes is essential to understand the emergence of complex behavioural phenotypes. Here, we describe the development of a de novo transcriptome and brain gene expression profiles of wild-caught peacock blenny, Salaria pavo, an intertidal fish with sex-role reversal in courtship behaviour (i.e., females are the courting sex) and sequential alternative reproductive tactics in males (i.e., larger and older nest-holder males and smaller and younger sneaker males occur). Sneakers mimic both female's courtship behaviour and nuptial coloration to get access to nests and sneak fertilizations, and later in life transition into nest-holder males. Thus, this species offers the unique opportunity to study how the regulation of gene expression can contribute to intersex phenotypes and to the sequential expression of male and female behavioural phenotypes by the same individual. We found that at the whole brain level, expression of the sneaker tactic was paralleled by broader and divergent gene expression when compared to either females or nest-holder males, which were more similar between themselves. When looking at sex-biased transcripts, sneaker males are intersex rather than being either nest-holder or female-like, and their transcriptome is simultaneously demasculinized for nest-holder-biased transcripts and feminized for female-biased transcripts. These results indicate that evolutionary changes in reproductive plasticity can be achieved through regulation of gene expression, and in particular by varying the magnitude of expression of sex-biased genes, throughout the lifetime of the same individual. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ispa.pt/bitstreams/d64b53e5-5dc8-4a4b-a149-57da0e812d63/download |
| funding.funder.alternateName_str_mv | FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.identifier_str_mv | EXCL/BIA-ANM/0549/2012 SFRH/BD/89072/2012 |
| funding.name_str_mv | 3599-PPCDT |
| funding_str_mv | EXCL/BIA-ANM/0549/2012 info:eu-repo/grantAgreement/FCT/3599-PPCDT/EXCL%2FBIA-ANM%2F0549%2F2012/PT SFRH/BD/89072/2012 info:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F89072%2F2012/PT |
| id | ispa_d2d9afa9fbe8cfb3fcfee62393116e8f |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.12/6986 |
| instacron_str | ispa |
| institution | Ispa-Instituto Universitário |
| instname_str | Ispa-Instituto Universitário |
| language | eng |
| network_acronym_str | ispa |
| network_name_str | Repositório do Ispa - Instituto Universitário |
| oai_identifier_str | oai:repositorio.ispa.pt:10400.12/6986 |
| organization_str_mv | urn:organizationAcronym:ispa |
| person_str_mv | Cardoso, Sara Cardoso, Sara https://www.ciencia-id.pt/991E-446C-750C 991E-446C-750C http://orcid.org/0000-0002-1006-2323 0000-0002-1006-2323 Gonçalves, David Goesmann, Alexander Goesmann, Alexander http://orcid.org/0000-0002-7086-2568 0000-0002-7086-2568 Canário, Adelino V. M. Oliveira, Rui Filipe |
| publishDate | 2018 |
| publisher.none.fl_str_mv | Blackwell Publishing Inc. |
| reponame_str | Repositório do Ispa - Instituto Universitário |
| repository_id_str | urn:repositoryAcronym:ispa |
| service_str_mv | urn:repositoryAcronym:ispa |
| spelling | engBlackwell Publishing Inc.pt_PTDistinct patterns of gene expression often underlie intra- and intersexual differences, and the study of this set of coregulated genes is essential to understand the emergence of complex behavioural phenotypes. Here, we describe the development of a de novo transcriptome and brain gene expression profiles of wild-caught peacock blenny, Salaria pavo, an intertidal fish with sex-role reversal in courtship behaviour (i.e., females are the courting sex) and sequential alternative reproductive tactics in males (i.e., larger and older nest-holder males and smaller and younger sneaker males occur). Sneakers mimic both female's courtship behaviour and nuptial coloration to get access to nests and sneak fertilizations, and later in life transition into nest-holder males. Thus, this species offers the unique opportunity to study how the regulation of gene expression can contribute to intersex phenotypes and to the sequential expression of male and female behavioural phenotypes by the same individual. We found that at the whole brain level, expression of the sneaker tactic was paralleled by broader and divergent gene expression when compared to either females or nest-holder males, which were more similar between themselves. When looking at sex-biased transcripts, sneaker males are intersex rather than being either nest-holder or female-like, and their transcriptome is simultaneously demasculinized for nest-holder-biased transcripts and feminized for female-biased transcripts. These results indicate that evolutionary changes in reproductive plasticity can be achieved through regulation of gene expression, and in particular by varying the magnitude of expression of sex-biased genes, throughout the lifetime of the same individual.application/pdfpt_PTTemporal variation in brain transcriptome is associated with the expression of female mimicry as a sequential male alternative reproductive tactic in fishPersonalCardoso, SaraDSpacehttp://dspace.org/items/00d0d929-5def-48bc-9b33-2b71381f2214DSpacehttp://dspace.org/items/00d0d929-5def-48bc-9b33-2b71381f2214CardosoSaraCiência IDhttps://www.ciencia-id.pt991E-446C-750CORCIDhttp://orcid.org0000-0002-1006-2323Researcher IDhttps://www.researcherid.comH-1372-2012Scopus Author IDhttps://www.scopus.com55889189700Scopus Author IDhttps://www.scopus.com58126196200Gonçalves, DavidPersonalGoesmann, AlexanderDSpacehttp://dspace.org/items/ad4f2c8f-9fe1-4f25-afa9-f97f8b710081DSpacehttp://dspace.org/items/ad4f2c8f-9fe1-4f25-afa9-f97f8b710081GoesmannAlexanderORCIDhttp://orcid.org0000-0002-7086-2568Canário, Adelino V. M.Oliveira, Rui FilipeHostingInstitutionOrganizationalRepositório do ISPAe-mailmailto:repositorio@ispa.ptrepositorio@ispa.ptISSNIsPartOf0962-1083DOIIsPartOf10.1111/mec.144082019-04-08T17:38:07Z20182018-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.12/6986http://purl.org/coar/access_right/c_16ecrestricted accessAlternative reproductive tacticsBehavioural plasticityNeurogenomic stateRNA-seqSalaria PavoSex-biased gene expression958276 bytesFundação para a Ciência e a TecnologiaMolecular mechanisms and evolutionary implications of social plasticityinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/EXCL%2FBIA-ANM%2F0549%2F2012/PTEXCL/BIA-ANM/0549/20123599-PPCDTCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaRNA-SEQ APPLIED TO THE PEACOCK BLENNY SALARIA PAVO: UNVEILING THE GENE NETWORKS AND SIGNALLING PATHWAYS BEHIND PHENOTYPIC PLASTICITY IN A LITTORAL FISHinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F89072%2F2012/PTSFRH/BD/89072/2012Crossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal article2018http://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ispa.pt/bitstreams/d64b53e5-5dc8-4a4b-a149-57da0e812d63/downloadMolecular ecology273789803United Kingdom |
| spellingShingle | Temporal variation in brain transcriptome is associated with the expression of female mimicry as a sequential male alternative reproductive tactic in fish Cardoso, Sara Alternative reproductive tactics Behavioural plasticity Neurogenomic state RNA-seq Salaria Pavo Sex-biased gene expression |
| status | SINGLETON |
| subject.fl_str_mv | Alternative reproductive tactics Behavioural plasticity Neurogenomic state RNA-seq Salaria Pavo Sex-biased gene expression |
| title | Temporal variation in brain transcriptome is associated with the expression of female mimicry as a sequential male alternative reproductive tactic in fish |
| title_full | Temporal variation in brain transcriptome is associated with the expression of female mimicry as a sequential male alternative reproductive tactic in fish |
| title_fullStr | Temporal variation in brain transcriptome is associated with the expression of female mimicry as a sequential male alternative reproductive tactic in fish |
| title_full_unstemmed | Temporal variation in brain transcriptome is associated with the expression of female mimicry as a sequential male alternative reproductive tactic in fish |
| title_short | Temporal variation in brain transcriptome is associated with the expression of female mimicry as a sequential male alternative reproductive tactic in fish |
| title_sort | Temporal variation in brain transcriptome is associated with the expression of female mimicry as a sequential male alternative reproductive tactic in fish |
| topic | Alternative reproductive tactics Behavioural plasticity Neurogenomic state RNA-seq Salaria Pavo Sex-biased gene expression |
| topic_facet | Alternative reproductive tactics Behavioural plasticity Neurogenomic state RNA-seq Salaria Pavo Sex-biased gene expression |
| url | http://hdl.handle.net/10400.12/6986 |
| visible | 1 |
Atividades financiadas
Carregando projetos financiados...