Publicação
Not out of the Mediterranean: Atlantic populations of the gorgonian Paramuricea clavata are a separate sister species under further lineage diversification
| Resumo: | The accurate delimitation of species boundaries in nonbilaterian marine taxa is notoriously difficult, with consequences for many studies in ecology and evolution. Anthozoans are a diverse group of key structural organisms worldwide, but the lack of reliable morphological characters and informative genetic markers hampers our ability to understand species diversification. We investigated population differentiation and species limits in Atlantic (Iberian Peninsula) and Mediterranean lineages of the octocoral genus Paramuricea previously identified as P. clavata. We used a diverse set of molecular markers (microsatellites, RNA-seq derived single-copy orthologues [SCO] and mt-mutS [mitochondrial barcode]) at 49 locations. Clear segregation of Atlantic and Mediterranean lineages was found with all markers. Species-tree estimations based on SCO strongly supported these two clades as distinct, recently diverged sister species with incomplete lineage sorting, P. cf. grayi and P. clavata, respectively. Furthermore, a second putative (or ongoing) speciation event was detected in the Atlantic between two P. cf. grayi color morphotypes (yellow and purple) using SCO and supported by microsatellites. While segregating P. cf. grayi lineages showed considerable geographic structure, dominating circalittoral communities in southern (yellow) and western (purple) Portugal, their occurrence in sympatry at some localities suggests a degree of reproductive isolation. Overall, our results show that previous molecular and morphological studies have underestimated species diversity in Paramuricea occurring in the Iberian Peninsula, which has important implications for conservation planning. Finally, our findings validate the usefulness of phylotranscriptomics for resolving evolutionary relationships in octocorals |
|---|---|
| Autores principais: | A. G. Coelho, Márcio |
| Outros Autores: | Pearson, Gareth Anthony; Boavida, Joana; Paulo, Diogo; Aurelle, Didier; ARNAUD-HAOND, Sophie; Gómez-Gras, Daniel; Bensoussan, Nathaniel; López-Sendino, Paula; Cerrano, Carlo; Kipson, Silvija; Bakran-Petricioli, Tatjana; Ferretti, Eliana; Linares, Cristina; Garrabou, Joaquim; Serrão, Ester A.; Ledoux, Jean-Baptiste |
| Assunto: | Atlantic-Mediterranean transition Cryptic diversity Incomplete lineage sorting Octocorallia Phylotranscriptomics Species delimitation |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Ispa-Instituto Universitário |
| Idioma: | inglês |
| Origem: | Repositório do Ispa - Instituto Universitário |
| _version_ | 1866887020682412032 |
|---|---|
| author | A. G. Coelho, Márcio |
| author2 | Pearson, Gareth Anthony Boavida, Joana Paulo, Diogo Aurelle, Didier ARNAUD-HAOND, Sophie Gómez-Gras, Daniel Bensoussan, Nathaniel López-Sendino, Paula Cerrano, Carlo Kipson, Silvija Bakran-Petricioli, Tatjana Ferretti, Eliana Linares, Cristina Garrabou, Joaquim Serrão, Ester A. Ledoux, Jean-Baptiste |
| author2_role | author author author author author author author author author author author author author author author author |
| author_facet | A. G. Coelho, Márcio Pearson, Gareth Anthony Boavida, Joana Paulo, Diogo Aurelle, Didier ARNAUD-HAOND, Sophie Gómez-Gras, Daniel Bensoussan, Nathaniel López-Sendino, Paula Cerrano, Carlo Kipson, Silvija Bakran-Petricioli, Tatjana Ferretti, Eliana Linares, Cristina Garrabou, Joaquim Serrão, Ester A. Ledoux, Jean-Baptiste |
| author_role | author |
| contributor_name_str_mv | Repositório do ISPA |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"A. G. Coelho, Márcio\",\"Person.identifier.orcid\":\"0000-0001-7832-5241\"},{\"Person.name\":\"Pearson, Gareth Anthony\",\"Person.identifier.orcid\":\"0000-0002-0768-464X\"},{\"Person.name\":\"Boavida, Joana\",\"Person.identifier.orcid\":\"0000-0001-5265-2498\"},{\"Person.name\":\"Paulo, Diogo\",\"Person.identifier.orcid\":\"0000-0001-9061-6850\"},{\"Person.name\":\"Aurelle, Didier\",\"Person.identifier.orcid\":\"0000-0002-3922-7291\"},{\"Person.name\":\"ARNAUD-HAOND, Sophie\",\"Person.identifier.orcid\":\"0000-0001-5814-8452\"},{\"Person.name\":\"Gómez-Gras, Daniel\",\"Person.identifier.orcid\":\"0000-0002-9738-6522\"},{\"Person.name\":\"Bensoussan, Nathaniel\"},{\"Person.name\":\"López-Sendino, Paula\",\"Person.identifier.orcid\":\"0000-0001-9786-8006\"},{\"Person.name\":\"Cerrano, Carlo\"},{\"Person.name\":\"Kipson, Silvija\",\"Person.identifier.orcid\":\"0000-0001-8313-9881\"},{\"Person.name\":\"Bakran-Petricioli, Tatjana\",\"Person.identifier.orcid\":\"0000-0002-3368-4248\"},{\"Person.name\":\"Ferretti, Eliana\",\"Person.identifier.orcid\":\"0000-0002-3159-1988\"},{\"Person.name\":\"Linares, Cristina\",\"Person.identifier.orcid\":\"0000-0003-3855-2743\"},{\"Person.name\":\"Garrabou, Joaquim\",\"Person.identifier.orcid\":\"0000-0001-9900-7277\"},{\"Person.name\":\"Serrão, Ester A.\"},{\"Person.name\":\"Ledoux, Jean-Baptiste\",\"Person.identifier.orcid\":\"0000-0001-8796-6163\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório do ISPA |
| datacite.creators.creator.creatorName.fl_str_mv | A. G. Coelho, Márcio Pearson, Gareth Anthony Boavida, Joana Paulo, Diogo Aurelle, Didier ARNAUD-HAOND, Sophie Gómez-Gras, Daniel Bensoussan, Nathaniel López-Sendino, Paula Cerrano, Carlo Kipson, Silvija Bakran-Petricioli, Tatjana Ferretti, Eliana Linares, Cristina Garrabou, Joaquim Serrão, Ester A. Ledoux, Jean-Baptiste |
| datacite.date.Accepted.fl_str_mv | 2023-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-07-12T17:58:50Z |
| datacite.date.embargoed.fl_str_mv | 2023-07-12T17:58:50Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Atlantic-Mediterranean transition Cryptic diversity Incomplete lineage sorting Octocorallia Phylotranscriptomics Species delimitation |
| datacite.titles.title.fl_str_mv | Not out of the Mediterranean: Atlantic populations of the gorgonian Paramuricea clavata are a separate sister species under further lineage diversification |
| dc.contributor.none.fl_str_mv | Repositório do ISPA |
| dc.creator.none.fl_str_mv | A. G. Coelho, Márcio Pearson, Gareth Anthony Boavida, Joana Paulo, Diogo Aurelle, Didier ARNAUD-HAOND, Sophie Gómez-Gras, Daniel Bensoussan, Nathaniel López-Sendino, Paula Cerrano, Carlo Kipson, Silvija Bakran-Petricioli, Tatjana Ferretti, Eliana Linares, Cristina Garrabou, Joaquim Serrão, Ester A. Ledoux, Jean-Baptiste |
| dc.date.Accepted.fl_str_mv | 2023-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-07-12T17:58:50Z |
| dc.date.embargoed.fl_str_mv | 2023-07-12T17:58:50Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.12/9219 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | John Wiley and Sons Ltd |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Atlantic-Mediterranean transition Cryptic diversity Incomplete lineage sorting Octocorallia Phylotranscriptomics Species delimitation |
| dc.title.fl_str_mv | Not out of the Mediterranean: Atlantic populations of the gorgonian Paramuricea clavata are a separate sister species under further lineage diversification |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The accurate delimitation of species boundaries in nonbilaterian marine taxa is notoriously difficult, with consequences for many studies in ecology and evolution. Anthozoans are a diverse group of key structural organisms worldwide, but the lack of reliable morphological characters and informative genetic markers hampers our ability to understand species diversification. We investigated population differentiation and species limits in Atlantic (Iberian Peninsula) and Mediterranean lineages of the octocoral genus Paramuricea previously identified as P. clavata. We used a diverse set of molecular markers (microsatellites, RNA-seq derived single-copy orthologues [SCO] and mt-mutS [mitochondrial barcode]) at 49 locations. Clear segregation of Atlantic and Mediterranean lineages was found with all markers. Species-tree estimations based on SCO strongly supported these two clades as distinct, recently diverged sister species with incomplete lineage sorting, P. cf. grayi and P. clavata, respectively. Furthermore, a second putative (or ongoing) speciation event was detected in the Atlantic between two P. cf. grayi color morphotypes (yellow and purple) using SCO and supported by microsatellites. While segregating P. cf. grayi lineages showed considerable geographic structure, dominating circalittoral communities in southern (yellow) and western (purple) Portugal, their occurrence in sympatry at some localities suggests a degree of reproductive isolation. Overall, our results show that previous molecular and morphological studies have underestimated species diversity in Paramuricea occurring in the Iberian Peninsula, which has important implications for conservation planning. Finally, our findings validate the usefulness of phylotranscriptomics for resolving evolutionary relationships in octocorals |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10400.12/9219 |
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| institution | Ispa-Instituto Universitário |
| instname_str | Ispa-Instituto Universitário |
| language | eng |
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| person_str_mv | A. G. Coelho, Márcio A. G. Coelho, Márcio https://www.ciencia-id.pt/F415-B297-7821 F415-B297-7821 http://orcid.org/0000-0001-7832-5241 0000-0001-7832-5241 Pearson, Gareth Anthony Pearson, Gareth Anthony https://www.ciencia-id.pt/3315-9919-1A52 3315-9919-1A52 http://orcid.org/0000-0002-0768-464X 0000-0002-0768-464X Boavida, Joana Boavida, Joana https://www.ciencia-id.pt/191A-5A62-0E81 191A-5A62-0E81 http://orcid.org/0000-0001-5265-2498 0000-0001-5265-2498 Paulo, Diogo Paulo, Diogo https://www.ciencia-id.pt/D31B-CCC9-EDBB D31B-CCC9-EDBB http://orcid.org/0000-0001-9061-6850 0000-0001-9061-6850 Aurelle, Didier Aurelle, Didier https://www.ciencia-id.pt/971D-90FA-8492 971D-90FA-8492 http://orcid.org/0000-0002-3922-7291 0000-0002-3922-7291 ARNAUD-HAOND, Sophie ARNAUD-HAOND, Sophie https://www.ciencia-id.pt/5A15-FF67-4075 5A15-FF67-4075 http://orcid.org/0000-0001-5814-8452 0000-0001-5814-8452 Gómez-Gras, Daniel Gómez-Gras, Daniel http://orcid.org/0000-0002-9738-6522 0000-0002-9738-6522 Bensoussan, Nathaniel López-Sendino, Paula López-Sendino, Paula http://orcid.org/0000-0001-9786-8006 0000-0001-9786-8006 Cerrano, Carlo Kipson, Silvija Kipson, Silvija http://orcid.org/0000-0001-8313-9881 0000-0001-8313-9881 Bakran-Petricioli, Tatjana Bakran-Petricioli, Tatjana http://orcid.org/0000-0002-3368-4248 0000-0002-3368-4248 Ferretti, Eliana Ferretti, Eliana http://orcid.org/0000-0002-3159-1988 0000-0002-3159-1988 Linares, Cristina Linares, Cristina https://www.ciencia-id.pt/8A1A-5ED6-4AE5 8A1A-5ED6-4AE5 http://orcid.org/0000-0003-3855-2743 0000-0003-3855-2743 Garrabou, Joaquim Garrabou, Joaquim https://www.ciencia-id.pt/DA15-8AC8-035C DA15-8AC8-035C http://orcid.org/0000-0001-9900-7277 0000-0001-9900-7277 Serrão, Ester A. Ledoux, Jean-Baptiste Ledoux, Jean-Baptiste https://www.ciencia-id.pt/0812-1710-4666 0812-1710-4666 http://orcid.org/0000-0001-8796-6163 0000-0001-8796-6163 |
| publishDate | 2023 |
| publisher.none.fl_str_mv | John Wiley and Sons Ltd |
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| spelling | engJohn Wiley and Sons Ltdpt_PTThe accurate delimitation of species boundaries in nonbilaterian marine taxa is notoriously difficult, with consequences for many studies in ecology and evolution. Anthozoans are a diverse group of key structural organisms worldwide, but the lack of reliable morphological characters and informative genetic markers hampers our ability to understand species diversification. We investigated population differentiation and species limits in Atlantic (Iberian Peninsula) and Mediterranean lineages of the octocoral genus Paramuricea previously identified as P. clavata. We used a diverse set of molecular markers (microsatellites, RNA-seq derived single-copy orthologues [SCO] and mt-mutS [mitochondrial barcode]) at 49 locations. Clear segregation of Atlantic and Mediterranean lineages was found with all markers. Species-tree estimations based on SCO strongly supported these two clades as distinct, recently diverged sister species with incomplete lineage sorting, P. cf. grayi and P. clavata, respectively. Furthermore, a second putative (or ongoing) speciation event was detected in the Atlantic between two P. cf. grayi color morphotypes (yellow and purple) using SCO and supported by microsatellites. While segregating P. cf. grayi lineages showed considerable geographic structure, dominating circalittoral communities in southern (yellow) and western (purple) Portugal, their occurrence in sympatry at some localities suggests a degree of reproductive isolation. Overall, our results show that previous molecular and morphological studies have underestimated species diversity in Paramuricea occurring in the Iberian Peninsula, which has important implications for conservation planning. Finally, our findings validate the usefulness of phylotranscriptomics for resolving evolutionary relationships in octocoralsapplication/pdfpt_PTNot out of the Mediterranean: Atlantic populations of the gorgonian Paramuricea clavata are a separate sister species under further lineage diversificationPersonalA. G. Coelho, MárcioDSpacehttp://dspace.org/items/2b43bad4-89be-4331-81d4-70acc2ba3bb5DSpacehttp://dspace.org/items/2b43bad4-89be-4331-81d4-70acc2ba3bb5A. G. CoelhoMárcioCiência IDhttps://www.ciencia-id.ptF415-B297-7821ORCIDhttp://orcid.org0000-0001-7832-5241Researcher IDhttps://www.researcherid.comE-7681-2016PersonalPearson, Gareth AnthonyDSpacehttp://dspace.org/items/21c0abce-164d-47da-bacd-c8daea7146e0DSpacehttp://dspace.org/items/21c0abce-164d-47da-bacd-c8daea7146e0PearsonGareth AnthonyCiência IDhttps://www.ciencia-id.pt3315-9919-1A52ORCIDhttp://orcid.org0000-0002-0768-464XResearcher IDhttps://www.researcherid.comJ-3911-2013Scopus Author IDhttps://www.scopus.com7201899326Scopus Author IDhttps://www.scopus.com55916875600PersonalBoavida, JoanaDSpacehttp://dspace.org/items/745a3f9c-e757-45f2-bafc-aefc129d7655DSpacehttp://dspace.org/items/745a3f9c-e757-45f2-bafc-aefc129d7655Ruela Heimburger BoavidaJoanaCiência IDhttps://www.ciencia-id.pt191A-5A62-0E81ORCIDhttp://orcid.org0000-0001-5265-2498PersonalPaulo, DiogoDSpacehttp://dspace.org/items/e2baa686-0e2a-4802-b421-55a15359b6cbDSpacehttp://dspace.org/items/e2baa686-0e2a-4802-b421-55a15359b6cbPauloDiogoCiência IDhttps://www.ciencia-id.ptD31B-CCC9-EDBBORCIDhttp://orcid.org0000-0001-9061-6850Researcher IDhttps://www.researcherid.comM-7522-2013PersonalAurelle, DidierDSpacehttp://dspace.org/items/9f65d5d4-692b-4325-b52c-554f68209beeDSpacehttp://dspace.org/items/9f65d5d4-692b-4325-b52c-554f68209beeAurelleDidierCiência IDhttps://www.ciencia-id.pt971D-90FA-8492ORCIDhttp://orcid.org0000-0002-3922-7291PersonalARNAUD-HAOND, SophieDSpacehttp://dspace.org/items/3b63c012-492e-4176-a691-1b8403653089DSpacehttp://dspace.org/items/3b63c012-492e-4176-a691-1b8403653089ARNAUD-HAONDSophieCiência IDhttps://www.ciencia-id.pt5A15-FF67-4075ORCIDhttp://orcid.org0000-0001-5814-8452Scopus Author IDhttps://www.scopus.com6602532118PersonalGómez-Gras, DanielDSpacehttp://dspace.org/items/7c6bff78-9ae6-431b-b4e6-d1eb7235b976DSpacehttp://dspace.org/items/7c6bff78-9ae6-431b-b4e6-d1eb7235b976Gómez-GrasDanielORCIDhttp://orcid.org0000-0002-9738-6522Scopus Author IDhttps://www.scopus.com57204852377Bensoussan, NathanielPersonalLópez-Sendino, PaulaDSpacehttp://dspace.org/items/4aab193f-dca8-4be5-bf76-9278f28e216eDSpacehttp://dspace.org/items/4aab193f-dca8-4be5-bf76-9278f28e216eLópez-SendinoPaulaORCIDhttp://orcid.org0000-0001-9786-8006Cerrano, CarloPersonalKipson, SilvijaDSpacehttp://dspace.org/items/efe95dc8-3749-415e-a24b-0f48cdecd5f8DSpacehttp://dspace.org/items/efe95dc8-3749-415e-a24b-0f48cdecd5f8KipsonSilvijaORCIDhttp://orcid.org0000-0001-8313-9881PersonalBakran-Petricioli, TatjanaDSpacehttp://dspace.org/items/1aa4dcc2-4c4d-48e9-bf9e-55ef32b5bbc8DSpacehttp://dspace.org/items/1aa4dcc2-4c4d-48e9-bf9e-55ef32b5bbc8Bakran-PetricioliTatjanaORCIDhttp://orcid.org0000-0002-3368-4248Scopus Author IDhttps://www.scopus.com6603252431PersonalFerretti, ElianaDSpacehttp://dspace.org/items/6f49a874-13f8-46bc-a110-bc760c29064dDSpacehttp://dspace.org/items/6f49a874-13f8-46bc-a110-bc760c29064dFerrettiElianaORCIDhttp://orcid.org0000-0002-3159-1988Scopus Author IDhttps://www.scopus.com57208935256PersonalLinares, CristinaDSpacehttp://dspace.org/items/342b5f0e-e596-40db-a000-68ea3af48c14DSpacehttp://dspace.org/items/342b5f0e-e596-40db-a000-68ea3af48c14LinaresCristinaCiência IDhttps://www.ciencia-id.pt8A1A-5ED6-4AE5ORCIDhttp://orcid.org0000-0003-3855-2743PersonalGarrabou, JoaquimDSpacehttp://dspace.org/items/1374550d-f7f6-41a1-bffd-9ca19af7f0cdDSpacehttp://dspace.org/items/1374550d-f7f6-41a1-bffd-9ca19af7f0cdGarrabouJoaquimCiência IDhttps://www.ciencia-id.ptDA15-8AC8-035CORCIDhttp://orcid.org0000-0001-9900-7277Serrão, Ester A.PersonalLedoux, Jean-BaptisteDSpacehttp://dspace.org/items/be16824e-8f5a-407c-8e6a-6228ee5411c8DSpacehttp://dspace.org/items/be16824e-8f5a-407c-8e6a-6228ee5411c8LedouxJean-BaptisteCiência IDhttps://www.ciencia-id.pt0812-1710-4666ORCIDhttp://orcid.org0000-0001-8796-6163HostingInstitutionOrganizationalRepositório do ISPAe-mailmailto:repositorio@ispa.ptrepositorio@ispa.ptISSNIsPartOf20457758DOIIsPartOf10.1002/ece3.97402023-07-12T17:58:50Z20232023-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.12/9219http://purl.org/coar/access_right/c_abf2open accessAtlantic-Mediterranean transitionCryptic diversityIncomplete lineage sortingOctocoralliaPhylotranscriptomicsSpecies delimitation4603017 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2023http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ispa.pt/bitstreams/d1864ed5-a8b8-41ce-ad27-a83cb8c10121/downloadEcology and Evolution131121United Kingdom |
| spellingShingle | Not out of the Mediterranean: Atlantic populations of the gorgonian Paramuricea clavata are a separate sister species under further lineage diversification A. G. Coelho, Márcio Atlantic-Mediterranean transition Cryptic diversity Incomplete lineage sorting Octocorallia Phylotranscriptomics Species delimitation |
| status | SINGLETON |
| subject.fl_str_mv | Atlantic-Mediterranean transition Cryptic diversity Incomplete lineage sorting Octocorallia Phylotranscriptomics Species delimitation |
| title | Not out of the Mediterranean: Atlantic populations of the gorgonian Paramuricea clavata are a separate sister species under further lineage diversification |
| title_full | Not out of the Mediterranean: Atlantic populations of the gorgonian Paramuricea clavata are a separate sister species under further lineage diversification |
| title_fullStr | Not out of the Mediterranean: Atlantic populations of the gorgonian Paramuricea clavata are a separate sister species under further lineage diversification |
| title_full_unstemmed | Not out of the Mediterranean: Atlantic populations of the gorgonian Paramuricea clavata are a separate sister species under further lineage diversification |
| title_short | Not out of the Mediterranean: Atlantic populations of the gorgonian Paramuricea clavata are a separate sister species under further lineage diversification |
| title_sort | Not out of the Mediterranean: Atlantic populations of the gorgonian Paramuricea clavata are a separate sister species under further lineage diversification |
| topic | Atlantic-Mediterranean transition Cryptic diversity Incomplete lineage sorting Octocorallia Phylotranscriptomics Species delimitation |
| topic_facet | Atlantic-Mediterranean transition Cryptic diversity Incomplete lineage sorting Octocorallia Phylotranscriptomics Species delimitation |
| url | http://hdl.handle.net/10400.12/9219 |
| visible | 1 |