Publication
Extraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansies
| Summary: | Introduction: Pansies (Viola×wittrockiana) are a rich source of natural antioxidants with beneficial effects on human health. Objetives: The aim of our study was to investigate solvents’ influence (water, methanol, water:acetone (6:4, v/v)) on the extraction of bioactive compounds and antioxidant activity of pansies extracts. Methods: The bioactive compounds analyzed were the following: flavonoids, hydrolysable tannins and monomeric anthocyanins, as well as total phenols by the total reducing capacity assay (TRC). The antioxidant activity was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and reducing power assays. A Principal Component Analysis (PCA) was performed to differentiate pansies extracts. Results: The solvents that yielded extracts with the highest contents of hydrolysable tannins and TRC were methanol and water:acetone (6:4, v/v). To extract the highest contents of monomeric anthocyanins, methanol should be used (5.93 mg Cy 3-glu/g flower, d.w), while for flavonoids, water:acetone (6:4, v/v) was the preferred yielding an extract with 115 mg QE/g flower d.w. Water turned out to be the least effective solvent, giving extracts with the lowest antioxidant activity. In addition, methanol or water:acetone extracts were clearly distinguished from aqueous ones through a PCA analysis. Conclusions: Our results show that the bioactive compounds and antioxidant activity of pansies’ extracts are affected by the solvent used. |
|---|---|
| Main Authors: | Fernandes, Luana |
| Other Authors: | Pereira, José Alberto; Saraiva, Jorge; Casal, Susana; Ramalhosa, Elsa |
| Subject: | Agriculture, Food and Veterinary Sciences |
| Year: | 2019 |
| Country: | Portugal |
| Document type: | article |
| Access type: | open access |
| Associated institution: | Instituto Politécnico de Viseu |
| Language: | English |
| Origin: | Millenium |
| _version_ | 1869977588571570176 |
|---|---|
| author | Fernandes, Luana |
| author2 | Pereira, José Alberto Saraiva, Jorge Casal, Susana Ramalhosa, Elsa |
| author2_role | author author author author |
| author_facet | Fernandes, Luana Pereira, José Alberto Saraiva, Jorge Casal, Susana Ramalhosa, Elsa |
| author_role | author |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Fernandes, Luana\"},{\"Person.name\":\"Pereira, José Alberto\"},{\"Person.name\":\"Saraiva, Jorge\"},{\"Person.name\":\"Casal, Susana\"},{\"Person.name\":\"Ramalhosa, Elsa\"}] |
| datacite.creators.creator.creatorName.fl_str_mv | Fernandes, Luana Pereira, José Alberto Saraiva, Jorge Casal, Susana Ramalhosa, Elsa |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Agriculture, Food and Veterinary Sciences |
| datacite.titles.title.fl_str_mv | Extraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansies Influencia de los solventes de extracción en el contenido de compuestos bioactivos y actividad antioxidante de las flores dos pensamientos Influência dos solventes de extração no contéudo em compostos bioactivos e actividade antioxidante de flores de amores-perfeitos |
| dc.creator.none.fl_str_mv | Fernandes, Luana Pereira, José Alberto Saraiva, Jorge Casal, Susana Ramalhosa, Elsa |
| dc.description.none.fl_str_mv | Introducción: Los pensamientos (Viola x wittrockiana) son una rica fuente de antioxidantes naturales con efectos beneficiosos sobre la salud humana. Objetivos: El objetivo del presente estudio fue evaluar la influencia de los solventes (agua, metanol y agua:acetona (6:4, v/v) en la extracción de compuestos bioactivos y actividad antioxidante de extractos de pensamientos. Métodos: Los compuestos bioactivos analizados fueron flavonoides, taninos hidrolizados y antocianinas monoméricas. También se analizaron los fenoles totales por el ensayo de capacidad reductora total. La actividad antioxidante fue evaluada por los métodos: secuestro del radical libre 2,2-difenil-1-picrilhidrazilo (DPPH) y poder reductor. El análisis de componentes principales (PCA) fue realizado para diferenciar los extractos de los pensamientos. Resultados: Los solventes que produjeron extractos con los mayores contenidos de taninos hidrolizados y capacidad reductora total fueron el metanol y agua:acetona (6:4, v/v). Los extractos con mayores contenidos de antocianinas monoméricas se obtuvieron utilizando metanol (5.93 mg Cy 3-glu/g peso seco). Para los flavonoides el agua: acetona (6:4, v / v) fue el solvente que hay dado los mejores valores de extracción (115 mg QE / g peso seco). El agua fue el solvente menos eficaz, obteniéndo se extractos con menor actividad antioxidante. Además, los extractos de metanol y agua:acetona se separaron claramente de los acuosos mediante el análisis por PCA. Conclusións: Los resultados mostraron que la extracción de compuestos bioactivos y la actividad antioxidante de los pensamientos son influenciadas por el solvente utilizado. Introdução: Amores-perfeitos (Viola×wittrockiana) são uma fonte rica de antioxidantes naturais com efeitos benéficos para a saúde humana. Objetivos: O objetivo do presente estudo foi investigar a influência dos solventes (água, metanol e água:acetona (6:4, v/v)) na extracção de compostos bioactivos e actividade antioxidante de extractos de amores-perfeitos. Métodos: Os compostos bioactivos analisados foram os seguintes: flavonóides, taninos hidrolisáveis e antocianinas monoméricas, bem como, os fenóis totais através da avaliação da capacidade redutora total. A actividade antioxidante foi avaliada pelos métodos de sequestro do radical livre 2,2-difenil-1-picrilhidrazilo (DPPH) e poder redutor. Uma análise de componentes principais (PCA) foi realizada para diferenciar os extractos dos amores-perfeitos. Resultados: Os solventes que produziram extractos com os maiores teores de taninos hidrolisáveis e capacidade redutora total foram os de metanol e água: acetona (6:4, v/v). Para extrair os maiores teores de antocianinas monoméricas deve-se usar metanol (5.93 mg Cy 3-glu/ g de massa seca), enquanto que para extrair flavonóides, a água:acetona (6:4, v/v) é preferível (115 mg QE/g de massa seca). A água mostrou ser o solvente menos eficaz, obtendo-se extractos com a menor actividade antioxidante. Para além disso, os extractos de metanol e água:acetona foram claramente separados dos aquosos através da análise por PCA. Conclusões: Os resultados mostraram que a extracção de compostos bioactivos e a actividade antioxidante de flores de amores-perfeitos são influenciadas pelo solvente usado. |
| dc.identifier.none.fl_str_mv | https://doi.org/10.29352/mill0208.08.00205 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Polytechnic Institute of Viseu (IPV) |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Agriculture, Food and Veterinary Sciences |
| dc.title.fl_str_mv | Extraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansies Influencia de los solventes de extracción en el contenido de compuestos bioactivos y actividad antioxidante de las flores dos pensamientos Influência dos solventes de extração no contéudo em compostos bioactivos e actividade antioxidante de flores de amores-perfeitos |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Introduction: Pansies (Viola×wittrockiana) are a rich source of natural antioxidants with beneficial effects on human health. Objetives: The aim of our study was to investigate solvents’ influence (water, methanol, water:acetone (6:4, v/v)) on the extraction of bioactive compounds and antioxidant activity of pansies extracts. Methods: The bioactive compounds analyzed were the following: flavonoids, hydrolysable tannins and monomeric anthocyanins, as well as total phenols by the total reducing capacity assay (TRC). The antioxidant activity was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and reducing power assays. A Principal Component Analysis (PCA) was performed to differentiate pansies extracts. Results: The solvents that yielded extracts with the highest contents of hydrolysable tannins and TRC were methanol and water:acetone (6:4, v/v). To extract the highest contents of monomeric anthocyanins, methanol should be used (5.93 mg Cy 3-glu/g flower, d.w), while for flavonoids, water:acetone (6:4, v/v) was the preferred yielding an extract with 115 mg QE/g flower d.w. Water turned out to be the least effective solvent, giving extracts with the lowest antioxidant activity. In addition, methanol or water:acetone extracts were clearly distinguished from aqueous ones through a PCA analysis. Conclusions: Our results show that the bioactive compounds and antioxidant activity of pansies’ extracts are affected by the solvent used. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| id | millenium_1393198e2860fb20fc861c4cde2280da |
| identifier.doi.fl_str_mv | https://doi.org/10.29352/mill0208.08.00205 |
| inst_facet_str | urn:organizationAcronym:ipv{{{_:::_}}}Instituto Politécnico de Viseu |
| instacron_str | ipv |
| institution | Instituto Politécnico de Viseu |
| instname_str | Instituto Politécnico de Viseu |
| language | eng |
| network_acronym_str | millenium |
| network_name_str | Millenium |
| oai_identifier_str | oai:ojs.revistas.rcaap.pt:article/14752 |
| organization_str_mv | urn:organizationAcronym:ipv |
| person_str_mv | Fernandes, Luana Pereira, José Alberto Saraiva, Jorge Casal, Susana Ramalhosa, Elsa |
| publishDate | 2019 |
| publisher.none.fl_str_mv | Polytechnic Institute of Viseu (IPV) |
| repo_facet_str | urn:repositoryAcronym:millenium{{{_:::_}}}Millenium |
| reponame_str | Millenium |
| repository_id_str | urn:repositoryAcronym:millenium |
| service_str_mv | urn:repositoryAcronym:millenium |
| spelling | Polytechnic Institute of Viseu (IPV)enIntroduction: Pansies (Viola×wittrockiana) are a rich source of natural antioxidants with beneficial effects on human health. Objetives: The aim of our study was to investigate solvents’ influence (water, methanol, water:acetone (6:4, v/v)) on the extraction of bioactive compounds and antioxidant activity of pansies extracts. Methods: The bioactive compounds analyzed were the following: flavonoids, hydrolysable tannins and monomeric anthocyanins, as well as total phenols by the total reducing capacity assay (TRC). The antioxidant activity was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and reducing power assays. A Principal Component Analysis (PCA) was performed to differentiate pansies extracts. Results: The solvents that yielded extracts with the highest contents of hydrolysable tannins and TRC were methanol and water:acetone (6:4, v/v). To extract the highest contents of monomeric anthocyanins, methanol should be used (5.93 mg Cy 3-glu/g flower, d.w), while for flavonoids, water:acetone (6:4, v/v) was the preferred yielding an extract with 115 mg QE/g flower d.w. Water turned out to be the least effective solvent, giving extracts with the lowest antioxidant activity. In addition, methanol or water:acetone extracts were clearly distinguished from aqueous ones through a PCA analysis. Conclusions: Our results show that the bioactive compounds and antioxidant activity of pansies’ extracts are affected by the solvent used.esIntroducción: Los pensamientos (Viola x wittrockiana) son una rica fuente de antioxidantes naturales con efectos beneficiosos sobre la salud humana. Objetivos: El objetivo del presente estudio fue evaluar la influencia de los solventes (agua, metanol y agua:acetona (6:4, v/v) en la extracción de compuestos bioactivos y actividad antioxidante de extractos de pensamientos. Métodos: Los compuestos bioactivos analizados fueron flavonoides, taninos hidrolizados y antocianinas monoméricas. También se analizaron los fenoles totales por el ensayo de capacidad reductora total. La actividad antioxidante fue evaluada por los métodos: secuestro del radical libre 2,2-difenil-1-picrilhidrazilo (DPPH) y poder reductor. El análisis de componentes principales (PCA) fue realizado para diferenciar los extractos de los pensamientos. Resultados: Los solventes que produjeron extractos con los mayores contenidos de taninos hidrolizados y capacidad reductora total fueron el metanol y agua:acetona (6:4, v/v). Los extractos con mayores contenidos de antocianinas monoméricas se obtuvieron utilizando metanol (5.93 mg Cy 3-glu/g peso seco). Para los flavonoides el agua: acetona (6:4, v / v) fue el solvente que hay dado los mejores valores de extracción (115 mg QE / g peso seco). El agua fue el solvente menos eficaz, obteniéndo se extractos con menor actividad antioxidante. Además, los extractos de metanol y agua:acetona se separaron claramente de los acuosos mediante el análisis por PCA. Conclusións: Los resultados mostraron que la extracción de compuestos bioactivos y la actividad antioxidante de los pensamientos son influenciadas por el solvente utilizado.ptIntrodução: Amores-perfeitos (Viola×wittrockiana) são uma fonte rica de antioxidantes naturais com efeitos benéficos para a saúde humana. Objetivos: O objetivo do presente estudo foi investigar a influência dos solventes (água, metanol e água:acetona (6:4, v/v)) na extracção de compostos bioactivos e actividade antioxidante de extractos de amores-perfeitos. Métodos: Os compostos bioactivos analisados foram os seguintes: flavonóides, taninos hidrolisáveis e antocianinas monoméricas, bem como, os fenóis totais através da avaliação da capacidade redutora total. A actividade antioxidante foi avaliada pelos métodos de sequestro do radical livre 2,2-difenil-1-picrilhidrazilo (DPPH) e poder redutor. Uma análise de componentes principais (PCA) foi realizada para diferenciar os extractos dos amores-perfeitos. Resultados: Os solventes que produziram extractos com os maiores teores de taninos hidrolisáveis e capacidade redutora total foram os de metanol e água: acetona (6:4, v/v). Para extrair os maiores teores de antocianinas monoméricas deve-se usar metanol (5.93 mg Cy 3-glu/ g de massa seca), enquanto que para extrair flavonóides, a água:acetona (6:4, v/v) é preferível (115 mg QE/g de massa seca). A água mostrou ser o solvente menos eficaz, obtendo-se extractos com a menor actividade antioxidante. Para além disso, os extractos de metanol e água:acetona foram claramente separados dos aquosos através da análise por PCA. Conclusões: Os resultados mostraram que a extracção de compostos bioactivos e a actividade antioxidante de flores de amores-perfeitos são influenciadas pelo solvente usado.engenExtraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansiesesInfluencia de los solventes de extracción en el contenido de compuestos bioactivos y actividad antioxidante de las flores dos pensamientosTranslatedTitleptInfluência dos solventes de extração no contéudo em compostos bioactivos e actividade antioxidante de flores de amores-perfeitosTranslatedTitleFernandes, LuanaFernandesLuana-Mountain Research Centre (CIMO) - School of Agriculture, Polytechnic Institute of Bragança~ -Organic Chemistry, Natural Products and Agrifood (QOPNA) – Chemistry Department, University of Aveiro - REQUIMTE/Laboratory of Bromatology and Hydrology, Faculty of Pharmacy, Porto UniversityPereira, José AlbertoPereiraJosé AlbertoMountain Research Centre (CIMO) - School of Agriculture, Polytechnic Institute of BragançaSaraiva, JorgeSaraivaJorgeOrganic Chemistry, Natural Products and Agrifood (QOPNA) – Chemistry Department, University of AveiroCasal, SusanaCasalSusanaREQUIMTE/Laboratory of Bromatology and Hydrology, Faculty of Pharmacy, Porto UniversityRamalhosa, ElsaRamalhosaElsaMountain Research Centre (CIMO) - School of Agriculture, Polytechnic Institute of BragançaEISSN1647-662XIsPartOfPISSN0873-3015IsPartOf2019-01-31DOIhttps://doi.org/10.29352/mill0208.08.00205http://purl.org/coar/access_right/c_abf2open accessAgriculture, Food and Veterinary Scienceshttp://purl.org/coar/resource_type/c_6501journal articlefulltextapplication/pdfMillenium - Journal of Education, Technologies, and Health88998 |
| spellingShingle | Extraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansies Fernandes, Luana Agriculture, Food and Veterinary Sciences |
| status | SINGLETON |
| subject.fl_str_mv | Agriculture, Food and Veterinary Sciences |
| title | Extraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansies |
| title_full | Extraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansies |
| title_fullStr | Extraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansies |
| title_full_unstemmed | Extraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansies |
| title_short | Extraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansies |
| title_sort | Extraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansies |
| topic | Agriculture, Food and Veterinary Sciences |
| topic_facet | Agriculture, Food and Veterinary Sciences |
| url | https://doi.org/10.29352/mill0208.08.00205 |
| visible | 1 |