Publicação
Evaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicity
| Resumo: | Quorum sensing (QS) is an important regulatory mechanism in biofilm formation and differentiation. Interference with QS can affect biofilm development and antimicrobial susceptibility. This study evaluates the potential of selected phytochemical products to inhibit QS. Three isothiocyanates (allylisothiocyanate - AITC, benzylisothiocyanate - BITC and 2-phenylethylisothiocyanate - PEITC) and six phenolic products (gallic acid - GA, ferulic acid - FA, caffeic acid - CA, phloridzin - PHL, (-) epicatechin - EPI and oleuropein glucoside - OG) were tested. A disc diffusion assay based on pigment inhibition in Chromobacterium violaceum CV12472 was performed. In addition, the mechanisms of QS inhibition (QSI) based on the modulation of N-acyl homoserine lactone (AHLs) activity and synthesis by the phytochemicals were investigated. The cytotoxicity of each product was tested on a cell line of mouse lung fibroblasts. AITC, BITC and PEITC demonstrated a capacity for QSI by modulation of AHL activity and synthesis, interfering the with QS systems of C. violaceum CviI/CviR homologs of LuxI/LuxR systems. The cytotoxic assays demonstrated low effects on the metabolic viability of the fibroblast cell line only for FA, PHL and EPI. |
|---|---|
| Autores principais: | Anabela Borges |
| Outros Autores: | Sofia Serra; Ana Cristina Abreu; Maria J. Saavedra; António Salgado; Manuel Simões |
| Assunto: | Ciências da Saúde, Ciências médicas e da saúde Health sciences, Medical and Health sciences |
| Ano: | 2014 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade do Porto |
| Idioma: | inglês |
| Origem: | Repositório Aberto da Universidade do Porto |
| _version_ | 1867767655674413056 |
|---|---|
| author | Anabela Borges |
| author2 | Sofia Serra Ana Cristina Abreu Maria J. Saavedra António Salgado Manuel Simões |
| author2_role | author author author author author |
| author_facet | Anabela Borges Sofia Serra Ana Cristina Abreu Maria J. Saavedra António Salgado Manuel Simões |
| author_role | author |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Anabela Borges\"},{\"Person.name\":\"Sofia Serra\"},{\"Person.name\":\"Ana Cristina Abreu\"},{\"Person.name\":\"Maria J. Saavedra\"},{\"Person.name\":\"António Salgado\"},{\"Person.name\":\"Manuel Simões\"}] |
| datacite.creators.creator.creatorName.fl_str_mv | Anabela Borges Sofia Serra Ana Cristina Abreu Maria J. Saavedra António Salgado Manuel Simões |
| datacite.date.Accepted.fl_str_mv | 2014-01-01T00:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Ciências da Saúde, Ciências médicas e da saúde Health sciences, Medical and Health sciences |
| datacite.titles.title.fl_str_mv | Evaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicity |
| dc.creator.none.fl_str_mv | Anabela Borges Sofia Serra Ana Cristina Abreu Maria J. Saavedra António Salgado Manuel Simões |
| dc.date.Accepted.fl_str_mv | 2014-01-01T00:00:00Z |
| dc.format.none.fl_str_mv | application/pdf application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/10216/103304 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Ciências da Saúde, Ciências médicas e da saúde Health sciences, Medical and Health sciences |
| dc.title.fl_str_mv | Evaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicity |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Quorum sensing (QS) is an important regulatory mechanism in biofilm formation and differentiation. Interference with QS can affect biofilm development and antimicrobial susceptibility. This study evaluates the potential of selected phytochemical products to inhibit QS. Three isothiocyanates (allylisothiocyanate - AITC, benzylisothiocyanate - BITC and 2-phenylethylisothiocyanate - PEITC) and six phenolic products (gallic acid - GA, ferulic acid - FA, caffeic acid - CA, phloridzin - PHL, (-) epicatechin - EPI and oleuropein glucoside - OG) were tested. A disc diffusion assay based on pigment inhibition in Chromobacterium violaceum CV12472 was performed. In addition, the mechanisms of QS inhibition (QSI) based on the modulation of N-acyl homoserine lactone (AHLs) activity and synthesis by the phytochemicals were investigated. The cytotoxicity of each product was tested on a cell line of mouse lung fibroblasts. AITC, BITC and PEITC demonstrated a capacity for QSI by modulation of AHL activity and synthesis, interfering the with QS systems of C. violaceum CviI/CviR homologs of LuxI/LuxR systems. The cytotoxic assays demonstrated low effects on the metabolic viability of the fibroblast cell line only for FA, PHL and EPI. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| id | rabertoup_fa2bc3c651f5d97cf7df6ea44bbb0839 |
| identifier.url.fl_str_mv | https://hdl.handle.net/10216/103304 |
| instacron_str | UP |
| institution | Universidade do Porto |
| instname_str | Universidade do Porto |
| language | eng |
| network_acronym_str | rabertoup |
| network_name_str | Repositório Aberto da Universidade do Porto |
| oai_identifier_str | oai:repositorio-aberto.up.pt:10216/103304 |
| organization_str_mv | urn:organizationAcronym:up |
| person_str_mv | Anabela Borges Sofia Serra Ana Cristina Abreu Maria J. Saavedra António Salgado Manuel Simões |
| publishDate | 2014 |
| reponame_str | Repositório Aberto da Universidade do Porto |
| repository_id_str | urn:repositoryAcronym:rabertoup |
| service_str_mv | urn:repositoryAcronym:rabertoup |
| spelling | Quorum sensing (QS) is an important regulatory mechanism in biofilm formation and differentiation. Interference with QS can affect biofilm development and antimicrobial susceptibility. This study evaluates the potential of selected phytochemical products to inhibit QS. Three isothiocyanates (allylisothiocyanate - AITC, benzylisothiocyanate - BITC and 2-phenylethylisothiocyanate - PEITC) and six phenolic products (gallic acid - GA, ferulic acid - FA, caffeic acid - CA, phloridzin - PHL, (-) epicatechin - EPI and oleuropein glucoside - OG) were tested. A disc diffusion assay based on pigment inhibition in Chromobacterium violaceum CV12472 was performed. In addition, the mechanisms of QS inhibition (QSI) based on the modulation of N-acyl homoserine lactone (AHLs) activity and synthesis by the phytochemicals were investigated. The cytotoxicity of each product was tested on a cell line of mouse lung fibroblasts. AITC, BITC and PEITC demonstrated a capacity for QSI by modulation of AHL activity and synthesis, interfering the with QS systems of C. violaceum CviI/CviR homologs of LuxI/LuxR systems. The cytotoxic assays demonstrated low effects on the metabolic viability of the fibroblast cell line only for FA, PHL and EPI.application/pdfapplication/pdfengEvaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicityAnabela BorgesSofia SerraAna Cristina AbreuMaria J. SaavedraAntónio SalgadoManuel SimõesHandlehttps://hdl.handle.net/10216/103304ISSNIsPartOf0892-7014DOIIsPartOf10.1080/08927014.2013.85254220142014-01-01T00:00:00Zhttp://purl.org/coar/access_right/c_16ecrestricted accessCiências da Saúde, Ciências médicas e da saúdeHealth sciences, Medical and Health sciences1089135 bytes748898 byteshttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio-aberto.up.pt/bitstream/10216/103304/2/105696.1.pdfhttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio-aberto.up.pt/bitstream/10216/103304/3/105696.pdfliteraturehttp://purl.org/coar/resource_type/c_6501journal article |
| spellingShingle | Evaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicity Anabela Borges Ciências da Saúde, Ciências médicas e da saúde Health sciences, Medical and Health sciences |
| status | SINGLETON |
| subject.fl_str_mv | Ciências da Saúde, Ciências médicas e da saúde Health sciences, Medical and Health sciences |
| title | Evaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicity |
| title_full | Evaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicity |
| title_fullStr | Evaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicity |
| title_full_unstemmed | Evaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicity |
| title_short | Evaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicity |
| title_sort | Evaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicity |
| topic | Ciências da Saúde, Ciências médicas e da saúde Health sciences, Medical and Health sciences |
| topic_facet | Ciências da Saúde, Ciências médicas e da saúde Health sciences, Medical and Health sciences |
| url | https://hdl.handle.net/10216/103304 |
| visible | 1 |