Publicação
Repeated exposure to ketamine in adolescent rats results in persistent anxiety in the adulthood
| Resumo: | Adolescent development of the prefrontal cortex (PFC) is accompanied by important changes in glutamatergic, GABAergic and dopaminergic circuitries, susceptible to modulation by N-methyl-D-aspartate receptors (NMDAR) antagonists. Repeated ketamine was associated with social and memory deficits, but other relevant factors, such as anxiety, were not sufficiently addressed. The present study aimed to examine the behavioral and molecular consequences of repeated exposure to ketamine with a particular focus in anxiety. Methods. We treated male adolescent Wistar rats, starting postnatal day (PND) 35, with ketamine (30 mg/kg, i.p, 7 days). Behavioral evaluation was conducted in the adulthood (PND 60). The elevated plus maze (EPM) and open field tests were used to evaluate anxiety and locomotion, while sociability and novelty recognition were assessed through the novel object recognition (NOR) and the sociability and social novelty tests. At the end of the behavioral evaluation, brains were dissected and the prefrontal cortex used for biochemical evaluation. Results. Analysis of the elevated plus maze (EPM) data revealed a ketamine-induced anxiety-like profile, corroborated by the open field data. Ketaminetreated rats also failed to increase contact time with a conspecific in the social affiliation test and with an unknown rat in the novelty preference test. At the molecular level, frontal expression levels of tyrosine hydroxylase were found decreased. Conclusion. Altogether, these results show that repeated ketamine-exposure in the adolescent may result in long-term anxiety |
|---|---|
| Autores principais: | Amorim, Manuela |
| Outros Autores: | Bravo, Joana; Silva, Ana Isabel; Alves, Cecília Juliana; Monteiro, Pedro; Magalhãess, Ana; Summavielle, Teresa |
| Assunto: | Ketamine NMDAR Adolescent Anxiety Schizophrenia Prefrontal cortex |
| Ano: | 2018 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico do Porto |
| Idioma: | inglês |
| Origem: | Repositório Científico do Instituto Politécnico do Porto |
| _version_ | 1868786040789008384 |
|---|---|
| author | Amorim, Manuela |
| author2 | Bravo, Joana Silva, Ana Isabel Alves, Cecília Juliana Monteiro, Pedro Magalhãess, Ana Summavielle, Teresa |
| author2_role | author author author author author author |
| author_facet | Amorim, Manuela Bravo, Joana Silva, Ana Isabel Alves, Cecília Juliana Monteiro, Pedro Magalhãess, Ana Summavielle, Teresa |
| author_role | author |
| contributor_name_str_mv | REPOSITÓRIO P.PORTO |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Amorim, Manuela\",\"Person.identifier.orcid\":\"0009-0006-1887-4840\"},{\"Person.name\":\"Bravo, Joana\"},{\"Person.name\":\"Silva, Ana Isabel\"},{\"Person.name\":\"Alves, Cecília Juliana\"},{\"Person.name\":\"Monteiro, Pedro\",\"Person.identifier.orcid\":\"0000-0002-3991-9191\"},{\"Person.name\":\"Magalhãess, Ana\"},{\"Person.name\":\"Summavielle, Teresa\",\"Person.identifier.orcid\":\"0000-0003-2548-6281\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | REPOSITÓRIO P.PORTO |
| datacite.creators.creator.creatorName.fl_str_mv | Amorim, Manuela Bravo, Joana Silva, Ana Isabel Alves, Cecília Juliana Monteiro, Pedro Magalhãess, Ana Summavielle, Teresa |
| datacite.date.Accepted.fl_str_mv | 2018-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2020-09-14T10:54:55Z |
| datacite.date.embargoed.fl_str_mv | 2020-09-14T10:54:55Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Ketamine NMDAR Adolescent Anxiety Schizophrenia Prefrontal cortex |
| datacite.titles.title.fl_str_mv | Repeated exposure to ketamine in adolescent rats results in persistent anxiety in the adulthood |
| dc.contributor.none.fl_str_mv | REPOSITÓRIO P.PORTO |
| dc.creator.none.fl_str_mv | Amorim, Manuela Bravo, Joana Silva, Ana Isabel Alves, Cecília Juliana Monteiro, Pedro Magalhãess, Ana Summavielle, Teresa |
| dc.date.Accepted.fl_str_mv | 2018-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2020-09-14T10:54:55Z |
| dc.date.embargoed.fl_str_mv | 2020-09-14T10:54:55Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.22/16251 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Ashdin Publishing |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Ketamine NMDAR Adolescent Anxiety Schizophrenia Prefrontal cortex |
| dc.title.fl_str_mv | Repeated exposure to ketamine in adolescent rats results in persistent anxiety in the adulthood |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Adolescent development of the prefrontal cortex (PFC) is accompanied by important changes in glutamatergic, GABAergic and dopaminergic circuitries, susceptible to modulation by N-methyl-D-aspartate receptors (NMDAR) antagonists. Repeated ketamine was associated with social and memory deficits, but other relevant factors, such as anxiety, were not sufficiently addressed. The present study aimed to examine the behavioral and molecular consequences of repeated exposure to ketamine with a particular focus in anxiety. Methods. We treated male adolescent Wistar rats, starting postnatal day (PND) 35, with ketamine (30 mg/kg, i.p, 7 days). Behavioral evaluation was conducted in the adulthood (PND 60). The elevated plus maze (EPM) and open field tests were used to evaluate anxiety and locomotion, while sociability and novelty recognition were assessed through the novel object recognition (NOR) and the sociability and social novelty tests. At the end of the behavioral evaluation, brains were dissected and the prefrontal cortex used for biochemical evaluation. Results. Analysis of the elevated plus maze (EPM) data revealed a ketamine-induced anxiety-like profile, corroborated by the open field data. Ketaminetreated rats also failed to increase contact time with a conspecific in the social affiliation test and with an unknown rat in the novelty preference test. At the molecular level, frontal expression levels of tyrosine hydroxylase were found decreased. Conclusion. Altogether, these results show that repeated ketamine-exposure in the adolescent may result in long-term anxiety |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://recipp.ipp.pt/bitstreams/e6006ba3-4c9a-46da-b5d3-03499f2ff7da/download |
| id | recipp_bc8e7c809cfdfcfef61a67ece407f522 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.22/16251 |
| instacron_str | recipp |
| institution | Instituto Politécnico do Porto |
| instname_str | Instituto Politécnico do Porto |
| language | eng |
| network_acronym_str | recipp |
| network_name_str | Repositório Científico do Instituto Politécnico do Porto |
| oai_identifier_str | oai:recipp.ipp.pt:10400.22/16251 |
| organization_str_mv | urn:organizationAcronym:recipp |
| person_str_mv | Amorim, Manuela Amorim, Manuela https://www.ciencia-id.pt/CD1D-1B47-A971 CD1D-1B47-A971 http://orcid.org/0009-0006-1887-4840 0009-0006-1887-4840 Bravo, Joana Silva, Ana Isabel Alves, Cecília Juliana Monteiro, Pedro Monteiro, Pedro https://www.ciencia-id.pt/2414-4468-928B 2414-4468-928B http://orcid.org/0000-0002-3991-9191 0000-0002-3991-9191 Magalhãess, Ana Summavielle, Teresa Summavielle, Teresa https://www.ciencia-id.pt/C41E-0816-5C85 C41E-0816-5C85 http://orcid.org/0000-0003-2548-6281 0000-0003-2548-6281 |
| publishDate | 2018 |
| publisher.none.fl_str_mv | Ashdin Publishing |
| reponame_str | Repositório Científico do Instituto Politécnico do Porto |
| repository_id_str | urn:repositoryAcronym:recipp |
| service_str_mv | urn:repositoryAcronym:recipp |
| spelling | engAshdin Publishingpt_PTAdolescent development of the prefrontal cortex (PFC) is accompanied by important changes in glutamatergic, GABAergic and dopaminergic circuitries, susceptible to modulation by N-methyl-D-aspartate receptors (NMDAR) antagonists. Repeated ketamine was associated with social and memory deficits, but other relevant factors, such as anxiety, were not sufficiently addressed. The present study aimed to examine the behavioral and molecular consequences of repeated exposure to ketamine with a particular focus in anxiety. Methods. We treated male adolescent Wistar rats, starting postnatal day (PND) 35, with ketamine (30 mg/kg, i.p, 7 days). Behavioral evaluation was conducted in the adulthood (PND 60). The elevated plus maze (EPM) and open field tests were used to evaluate anxiety and locomotion, while sociability and novelty recognition were assessed through the novel object recognition (NOR) and the sociability and social novelty tests. At the end of the behavioral evaluation, brains were dissected and the prefrontal cortex used for biochemical evaluation. Results. Analysis of the elevated plus maze (EPM) data revealed a ketamine-induced anxiety-like profile, corroborated by the open field data. Ketaminetreated rats also failed to increase contact time with a conspecific in the social affiliation test and with an unknown rat in the novelty preference test. At the molecular level, frontal expression levels of tyrosine hydroxylase were found decreased. Conclusion. Altogether, these results show that repeated ketamine-exposure in the adolescent may result in long-term anxietyapplication/pdfpt_PTRepeated exposure to ketamine in adolescent rats results in persistent anxiety in the adulthoodPersonalAmorim, ManuelaDSpacehttp://dspace.org/items/10df22ed-9823-452e-89a8-8cbe89e717ecDSpacehttp://dspace.org/items/10df22ed-9823-452e-89a8-8cbe89e717ecAmorim SousaMaria ManuelaCiência IDhttps://www.ciencia-id.ptCD1D-1B47-A971ORCIDhttp://orcid.org0009-0006-1887-4840Bravo, JoanaSilva, Ana IsabelAlves, Cecília JulianaPersonalMonteiro, PedroDSpacehttp://dspace.org/items/fae8b5b3-db39-4d56-a2b1-2db0388279b9DSpacehttp://dspace.org/items/fae8b5b3-db39-4d56-a2b1-2db0388279b9MonteiroPedroCiência IDhttps://www.ciencia-id.pt2414-4468-928BORCIDhttp://orcid.org0000-0002-3991-9191Scopus Author IDhttps://www.scopus.com6504494827Scopus Author IDhttps://www.scopus.com57192572968Magalhãess, AnaPersonalSummavielle, TeresaDSpacehttp://dspace.org/items/207ee2de-85a0-4144-9e7e-b376c600e065DSpacehttp://dspace.org/items/207ee2de-85a0-4144-9e7e-b376c600e065SummavielleTeresaCiência IDhttps://www.ciencia-id.ptC41E-0816-5C85ORCIDhttp://orcid.org0000-0003-2548-6281Researcher IDhttps://www.researcherid.comC-9776-2012Scopus Author IDhttps://www.scopus.com6603092949HostingInstitutionOrganizationalREPOSITÓRIO P.PORTOe-mailmailto:recipp@sc.ipp.ptrecipp@sc.ipp.ptDOIIsPartOf10.4303/jdar/2360602020-09-14T10:54:55Z20182018-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.22/16251http://purl.org/coar/access_right/c_abf2open accessKetamineNMDARAdolescentAnxietySchizophreniaPrefrontal cortex1132789 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://recipp.ipp.pt/bitstreams/e6006ba3-4c9a-46da-b5d3-03499f2ff7da/downloadJournal of drug and alcohol research0701 |
| spellingShingle | Repeated exposure to ketamine in adolescent rats results in persistent anxiety in the adulthood Amorim, Manuela Ketamine NMDAR Adolescent Anxiety Schizophrenia Prefrontal cortex |
| status | SINGLETON |
| subject.fl_str_mv | Ketamine NMDAR Adolescent Anxiety Schizophrenia Prefrontal cortex |
| title | Repeated exposure to ketamine in adolescent rats results in persistent anxiety in the adulthood |
| title_full | Repeated exposure to ketamine in adolescent rats results in persistent anxiety in the adulthood |
| title_fullStr | Repeated exposure to ketamine in adolescent rats results in persistent anxiety in the adulthood |
| title_full_unstemmed | Repeated exposure to ketamine in adolescent rats results in persistent anxiety in the adulthood |
| title_short | Repeated exposure to ketamine in adolescent rats results in persistent anxiety in the adulthood |
| title_sort | Repeated exposure to ketamine in adolescent rats results in persistent anxiety in the adulthood |
| topic | Ketamine NMDAR Adolescent Anxiety Schizophrenia Prefrontal cortex |
| topic_facet | Ketamine NMDAR Adolescent Anxiety Schizophrenia Prefrontal cortex |
| url | http://hdl.handle.net/10400.22/16251 |
| visible | 1 |