Publicação
Optimisation of a molecular methodology for the detection of virulence factors of enterotoxigenic Escherichia coli for the diagnosis of swine colibacillosis
| Resumo: | The most common bacterial pathogen causing enteric infections in pigs is enterotoxigenic Escherichia coli (ETEC). Since pigs represent the largest livestock category in the European Union, ETEC-associated diseases, better known as swine colibacillosis leading to acute diarrhea and eventual death of the animal, result in significant costs to the pig industry. These diseases are traditionally prevented or treated with antibiotics, and this has had a huge impact on the emergence of resistant bacteria, correlating with the emergence of resistant infections in humans. Recognition of this problem has led the authorities to set ambitious goals for the reduction of this type of drug in animal husbandry, leading to the creation of a national project, APTAcoli, which aims to select aptamers (consisting of small single-stranded oligonucleotides capable of binding to target molecules with great affinity and specificity, due to the specific secondary and/ or tertiary structures they can acquire) as an alternative in the treatment of colibacillosis. The present experimental study, which is on the APTAcoli agenda, focused on the optimization of a molecular methodology - Multiplex PCR - for the detection of the main virulence factors of ETEC to be used in an epidemiological study to characterize fecal samples from pigs in Portuguese farms. After using different optimization techniques, the results were two multiplex PCR amplification sets, one for amplification of the main toxigenic factors of ETEC (STa, STb, LT and STx2e) and another for amplification of the main adhesion factors (F4, F5, F6, F18 and F41). |
|---|---|
| Autores principais: | Campos, Ana |
| Outros Autores: | Oliveira, Ricardo; Almeida, Carina; Vieira, Filipa Quintela; Silva, Regina Augusta |
| Assunto: | ETEC Escherichia coli Swine colibacillosis Multiplex PCR |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico do Porto |
| Idioma: | inglês |
| Origem: | Repositório Científico do Instituto Politécnico do Porto |
| _version_ | 1868785995524079616 |
|---|---|
| author | Campos, Ana |
| author2 | Oliveira, Ricardo Almeida, Carina Vieira, Filipa Quintela Silva, Regina Augusta |
| author2_role | author author author author |
| author_facet | Campos, Ana Oliveira, Ricardo Almeida, Carina Vieira, Filipa Quintela Silva, Regina Augusta |
| author_role | author |
| contributor_name_str_mv | REPOSITÓRIO P.PORTO |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Campos, Ana\"},{\"Person.name\":\"Oliveira, Ricardo\"},{\"Person.name\":\"Almeida, Carina\"},{\"Person.name\":\"Vieira, Filipa Quintela\",\"Person.identifier.orcid\":\"0000-0003-0130-7664\"},{\"Person.name\":\"Silva, Regina Augusta\",\"Person.identifier.orcid\":\"0000-0002-8373-1217\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | REPOSITÓRIO P.PORTO |
| datacite.creators.creator.creatorName.fl_str_mv | Campos, Ana Oliveira, Ricardo Almeida, Carina Vieira, Filipa Quintela Silva, Regina Augusta |
| datacite.date.Accepted.fl_str_mv | 2022-10-21T00:00:00Z |
| datacite.date.available.fl_str_mv | 2024-06-07T08:59:49Z |
| datacite.date.embargoed.fl_str_mv | 2024-06-07T08:59:49Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | ETEC Escherichia coli Swine colibacillosis Multiplex PCR |
| datacite.titles.title.fl_str_mv | Optimisation of a molecular methodology for the detection of virulence factors of enterotoxigenic Escherichia coli for the diagnosis of swine colibacillosis |
| dc.contributor.none.fl_str_mv | REPOSITÓRIO P.PORTO |
| dc.creator.none.fl_str_mv | Campos, Ana Oliveira, Ricardo Almeida, Carina Vieira, Filipa Quintela Silva, Regina Augusta |
| dc.date.Accepted.fl_str_mv | 2022-10-21T00:00:00Z |
| dc.date.available.fl_str_mv | 2024-06-07T08:59:49Z |
| dc.date.embargoed.fl_str_mv | 2024-06-07T08:59:49Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.22/25645 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Escola Superior de Saúde P.Porto |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | ETEC Escherichia coli Swine colibacillosis Multiplex PCR |
| dc.title.fl_str_mv | Optimisation of a molecular methodology for the detection of virulence factors of enterotoxigenic Escherichia coli for the diagnosis of swine colibacillosis |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | The most common bacterial pathogen causing enteric infections in pigs is enterotoxigenic Escherichia coli (ETEC). Since pigs represent the largest livestock category in the European Union, ETEC-associated diseases, better known as swine colibacillosis leading to acute diarrhea and eventual death of the animal, result in significant costs to the pig industry. These diseases are traditionally prevented or treated with antibiotics, and this has had a huge impact on the emergence of resistant bacteria, correlating with the emergence of resistant infections in humans. Recognition of this problem has led the authorities to set ambitious goals for the reduction of this type of drug in animal husbandry, leading to the creation of a national project, APTAcoli, which aims to select aptamers (consisting of small single-stranded oligonucleotides capable of binding to target molecules with great affinity and specificity, due to the specific secondary and/ or tertiary structures they can acquire) as an alternative in the treatment of colibacillosis. The present experimental study, which is on the APTAcoli agenda, focused on the optimization of a molecular methodology - Multiplex PCR - for the detection of the main virulence factors of ETEC to be used in an epidemiological study to characterize fecal samples from pigs in Portuguese farms. After using different optimization techniques, the results were two multiplex PCR amplification sets, one for amplification of the main toxigenic factors of ETEC (STa, STb, LT and STx2e) and another for amplification of the main adhesion factors (F4, F5, F6, F18 and F41). |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | conferenceObject |
| fulltext.url.fl_str_mv | https://recipp.ipp.pt/bitstreams/c6f0c4a9-4ec5-4e1c-9c51-ad7ff562535a/download |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10400.22/25645 |
| instacron_str | recipp |
| institution | Instituto Politécnico do Porto |
| instname_str | Instituto Politécnico do Porto |
| language | eng |
| network_acronym_str | recipp |
| network_name_str | Repositório Científico do Instituto Politécnico do Porto |
| oai_identifier_str | oai:recipp.ipp.pt:10400.22/25645 |
| organization_str_mv | urn:organizationAcronym:recipp |
| person_str_mv | Campos, Ana Oliveira, Ricardo Almeida, Carina Vieira, Filipa Quintela Vieira, Filipa Quintela http://orcid.org/0000-0003-0130-7664 0000-0003-0130-7664 Silva, Regina Augusta Silva, Regina Augusta https://www.ciencia-id.pt/871B-D071-507D 871B-D071-507D http://orcid.org/0000-0002-8373-1217 0000-0002-8373-1217 |
| publishDate | 2022 |
| publisher.none.fl_str_mv | Escola Superior de Saúde P.Porto |
| reponame_str | Repositório Científico do Instituto Politécnico do Porto |
| repository_id_str | urn:repositoryAcronym:recipp |
| service_str_mv | urn:repositoryAcronym:recipp |
| spelling | engEscola Superior de Saúde P.Portopt_PTThe most common bacterial pathogen causing enteric infections in pigs is enterotoxigenic Escherichia coli (ETEC). Since pigs represent the largest livestock category in the European Union, ETEC-associated diseases, better known as swine colibacillosis leading to acute diarrhea and eventual death of the animal, result in significant costs to the pig industry. These diseases are traditionally prevented or treated with antibiotics, and this has had a huge impact on the emergence of resistant bacteria, correlating with the emergence of resistant infections in humans. Recognition of this problem has led the authorities to set ambitious goals for the reduction of this type of drug in animal husbandry, leading to the creation of a national project, APTAcoli, which aims to select aptamers (consisting of small single-stranded oligonucleotides capable of binding to target molecules with great affinity and specificity, due to the specific secondary and/ or tertiary structures they can acquire) as an alternative in the treatment of colibacillosis. The present experimental study, which is on the APTAcoli agenda, focused on the optimization of a molecular methodology - Multiplex PCR - for the detection of the main virulence factors of ETEC to be used in an epidemiological study to characterize fecal samples from pigs in Portuguese farms. After using different optimization techniques, the results were two multiplex PCR amplification sets, one for amplification of the main toxigenic factors of ETEC (STa, STb, LT and STx2e) and another for amplification of the main adhesion factors (F4, F5, F6, F18 and F41).application/pdfpt_PTOptimisation of a molecular methodology for the detection of virulence factors of enterotoxigenic Escherichia coli for the diagnosis of swine colibacillosisCampos, AnaOliveira, RicardoAlmeida, CarinaPersonalVieira, Filipa QuintelaDSpacehttp://dspace.org/items/47123d10-a0ed-47cc-a751-721489a13446DSpacehttp://dspace.org/items/47123d10-a0ed-47cc-a751-721489a13446Quintela VieiraAna FilipaORCIDhttp://orcid.org0000-0003-0130-7664PersonalSilva, Regina AugustaDSpacehttp://dspace.org/items/60471846-7876-49e1-b405-6ffe770795aeDSpacehttp://dspace.org/items/60471846-7876-49e1-b405-6ffe770795aeSilvaReginaCiência IDhttps://www.ciencia-id.pt871B-D071-507DORCIDhttp://orcid.org0000-0002-8373-1217HostingInstitutionOrganizationalREPOSITÓRIO P.PORTOe-mailmailto:recipp@sc.ipp.ptrecipp@sc.ipp.pt2024-06-07T08:59:49Z2022-10-212022-10-21T00:00:00ZHandlehttp://hdl.handle.net/10400.22/25645http://purl.org/coar/access_right/c_abf2open accessETECEscherichia coliSwine colibacillosisMultiplex PCR5392651 bytesother research producthttp://purl.org/coar/resource_type/c_c94fconference objecthttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://recipp.ipp.pt/bitstreams/c6f0c4a9-4ec5-4e1c-9c51-ad7ff562535a/downloadBook of Abstracts of 5th Meeting of Medicinal Biotechnology (5MBtM) and 2nd Iberian Congress on Medicinal Biotechnology67Porto |
| spellingShingle | Optimisation of a molecular methodology for the detection of virulence factors of enterotoxigenic Escherichia coli for the diagnosis of swine colibacillosis Campos, Ana ETEC Escherichia coli Swine colibacillosis Multiplex PCR |
| status | SINGLETON |
| subject.fl_str_mv | ETEC Escherichia coli Swine colibacillosis Multiplex PCR |
| title | Optimisation of a molecular methodology for the detection of virulence factors of enterotoxigenic Escherichia coli for the diagnosis of swine colibacillosis |
| title_full | Optimisation of a molecular methodology for the detection of virulence factors of enterotoxigenic Escherichia coli for the diagnosis of swine colibacillosis |
| title_fullStr | Optimisation of a molecular methodology for the detection of virulence factors of enterotoxigenic Escherichia coli for the diagnosis of swine colibacillosis |
| title_full_unstemmed | Optimisation of a molecular methodology for the detection of virulence factors of enterotoxigenic Escherichia coli for the diagnosis of swine colibacillosis |
| title_short | Optimisation of a molecular methodology for the detection of virulence factors of enterotoxigenic Escherichia coli for the diagnosis of swine colibacillosis |
| title_sort | Optimisation of a molecular methodology for the detection of virulence factors of enterotoxigenic Escherichia coli for the diagnosis of swine colibacillosis |
| topic | ETEC Escherichia coli Swine colibacillosis Multiplex PCR |
| topic_facet | ETEC Escherichia coli Swine colibacillosis Multiplex PCR |
| url | http://hdl.handle.net/10400.22/25645 |
| visible | 1 |