Publicação
Impact of moderate exercise training on heart mitochondrial acetylome
| Resumo: | Physical activity has been suggested to have a cardioprotective role, which seems to be related with mitochondrial adaptation. Aiming a better understanding of the impact of exercise training on heart functionality and, specifically, on the regulation of mitochondrial activity, in the present work we applied a mass spectrometry-based proteomic approach for the characterization of mitochondrial proteome adaptation to 54 weeks of moderate treadmill exercise. Focus was given to mitochondrial acetylome and so, enrichment strategies based on immunoprecipitation with anti-acetyl-lysine and anti-SIRT3 antibodies were used. Data highlighted the beneficial impact of 54-weeks of treadmill exercise on cardiac functionality. The analysis of mitochondria isolated from rat heart evidenced an exercise-related greater ability to produce ATP. Concomitantly, the mitochondrial expression of SIRT3 increased in exercised animals and was paralleled by alterations in the profile of acetylated proteins in heart mitochondria. These acetylated proteins are involved in a wide range of biological process being of notice the metabolism, which becomes more dependent on carbohydrates metabolism with lifelong exercise training. Curiously, none of the identified acetylated proteins are targets of SIRT3, according to STRING bioinformatics tool. Immunoprecipitation with anti-SIRT3 followed by LC-MS/MS did not allowed the identification of SIRT3 substrates. In the future, it will be important the identification of the enzymes involved in the regulation of mitochondrial acetylome besides SIRT3 to better characterize the impact of this posttranslational modification on the biological processes activated by lifestyle. |
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| Autores principais: | Azevedo, Ana Lúcia Barbosa |
| Assunto: | Bioquímica Exercício físico Doenças cardiovasculares |
| Ano: | 2014 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Aveiro |
| Idioma: | inglês |
| Origem: | RIA - Repositório Institucional da Universidade de Aveiro |
| Resumo: | Physical activity has been suggested to have a cardioprotective role, which seems to be related with mitochondrial adaptation. Aiming a better understanding of the impact of exercise training on heart functionality and, specifically, on the regulation of mitochondrial activity, in the present work we applied a mass spectrometry-based proteomic approach for the characterization of mitochondrial proteome adaptation to 54 weeks of moderate treadmill exercise. Focus was given to mitochondrial acetylome and so, enrichment strategies based on immunoprecipitation with anti-acetyl-lysine and anti-SIRT3 antibodies were used. Data highlighted the beneficial impact of 54-weeks of treadmill exercise on cardiac functionality. The analysis of mitochondria isolated from rat heart evidenced an exercise-related greater ability to produce ATP. Concomitantly, the mitochondrial expression of SIRT3 increased in exercised animals and was paralleled by alterations in the profile of acetylated proteins in heart mitochondria. These acetylated proteins are involved in a wide range of biological process being of notice the metabolism, which becomes more dependent on carbohydrates metabolism with lifelong exercise training. Curiously, none of the identified acetylated proteins are targets of SIRT3, according to STRING bioinformatics tool. Immunoprecipitation with anti-SIRT3 followed by LC-MS/MS did not allowed the identification of SIRT3 substrates. In the future, it will be important the identification of the enzymes involved in the regulation of mitochondrial acetylome besides SIRT3 to better characterize the impact of this posttranslational modification on the biological processes activated by lifestyle. |
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