Publicação
Photoresponsive hydrogels as a therapeutic platform for cancer treatment
| Resumo: | Targeted therapies for tumour treatment have emerged as promising approaches for inducing effective anti-tumour responses while reducing side effects in healthy tissues. However, the low bioavailability of many of the compounds used in these therapeutic strategies continues to limit their clinical application. In order to overcome these limitations, the main objective of this dissertation was to develop a new polymeric formulation in its native and acrylated form for the formation of hydrogels. The incorporation of porphyrin derivatives in these platforms was also promoted through covalent bonding, thus constituting a multifunctional system. To achieve this objective, porphyrin derivatives were prepared and subsequently functionalised with methacrylate derivative, obtaining porphyrin derivatives with different degrees of substitution. Similarly, the polymer matrix was functionalised and the conjugation between acrylated and non-acrylated polymer matrices with porphyrin derivatives was promoted, obtaining polymer matrices conjugated with porphyrins. All porphyrin derivatives and polymer conjugates prepared were subjected to structural characterisation by ¹H, ¹³C and ¹⁹F NMR and, whenever possible, by mass spectrometry and elemental analysis, thus confirming the structures obtained and the incorporation/conjugation of porphyrin into the polymer matrix. Finally, several hydrogel formulations were prepared based on the polymer matrices conjugated with porphyrin in order to evaluate the behaviour of the hydrogel and the influence of the composition on the photoactive response and properties of the material. Analysis of |
|---|---|
| Autores principais: | Olival, Telma Bregieiro |
| Assunto: | Hydrogels Porphyrins Polimeric matrices Tumors |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso embargado |
| Instituição associada: | Universidade de Aveiro |
| Idioma: | inglês |
| Origem: | RIA - Repositório Institucional da Universidade de Aveiro |
| _version_ | 1866173323286675456 |
|---|---|
| author | Olival, Telma Bregieiro |
| author_facet | Olival, Telma Bregieiro |
| author_role | author |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Olival, Telma Bregieiro\"}] |
| datacite.creators.creator.creatorName.fl_str_mv | Olival, Telma Bregieiro |
| datacite.date.Accepted.fl_str_mv | 2025-12-10T00:00:00Z |
| datacite.date.available.fl_str_mv | 2027-12-10T00:00:00Z |
| datacite.date.embargoed.fl_str_mv | 2027-12-10T00:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_f1cf |
| datacite.subjects.subject.fl_str_mv | Hydrogels Porphyrins Polimeric matrices Tumors |
| datacite.titles.title.fl_str_mv | Photoresponsive hydrogels as a therapeutic platform for cancer treatment |
| dc.creator.none.fl_str_mv | Olival, Telma Bregieiro |
| dc.date.Accepted.fl_str_mv | 2025-12-10T00:00:00Z |
| dc.date.available.fl_str_mv | 2027-12-10T00:00:00Z |
| dc.date.embargoed.fl_str_mv | 2027-12-10T00:00:00Z |
| dc.description.none.fl_str_mv | As terapias direcionadas para o tratamento de tumores têm vindo a afirmar-se como abordagens promissoras para induzir respostas anti-tumorais eficazes, reduzindo os efeitos secundários em tecidos saudáveis. No entanto, a baixa biodisponibilidade de muitos dos compostos utilizados nestas estratégias terapêuticas continua a limitar a sua aplicação clínica. No sentido de colmatar estas limitações, esta dissertação teve como objetivo principal o desenvolvimento de uma nova formulação polimérica na sua forma nativa e acrilatada, para a formação de hidrogéis. Foi também promovida a incorporação de derivados porfirínicos nestas plataformas através de ligação covalente, constituindo assim um sistema multifuncional. Para atingir este objetivo, foram preparados derivados porfirínicos que posteriormente foram funcionalizados com metacrilato, obtendo-se derivados porfirínicos com diferentes graus de substituição. Analogamente, funcionalizou-se a matriz polimérica e promoveu-se a conjugação entre as matrizes poliméricas acrilatadas e não acrilatadas com os derivados porfirínicos, obtendo-se matrizes poliméricas conjugadas com porfirinas. Todos os derivados porfirínicos e conjugados poliméricos preparados foram sujeitos a caracterização estrutural por RMN de ¹H, ¹³C e ¹⁹F e ainda sempre que possível por espetrometria de massa e análise elementar, confirmando desta forma as estruturas obtidas e a incorporação/conjugação da porfirina na matriz polimérica. Finalmente, foram preparadas várias formulações de hidrogéis com base nas matrizes poliméricas conjugadas com porfirina, de modo a avaliar o comportamento do hidrogel e a influência da composição na resposta fotoativa e nas propriedades do material. A análise das propriedades fotoquímicas e fotofísicas dos derivados preparados demonstrou que nem a funcionalização da porfirina nem a sua conjugação à matriz polimérica afetou as propriedades óticas, comprovando a preservação da estrutura eletrónica da porfirina após conjugação. Todos os derivados porfirínicos demonstram ainda manter as propriedades emissivas mesmo quando imobilizadas no hidrogel em estado sólido. |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10773/46607 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_f1cf |
| dc.subject.none.fl_str_mv | Hydrogels Porphyrins Polimeric matrices Tumors |
| dc.title.fl_str_mv | Photoresponsive hydrogels as a therapeutic platform for cancer treatment |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_bdcc |
| description | Targeted therapies for tumour treatment have emerged as promising approaches for inducing effective anti-tumour responses while reducing side effects in healthy tissues. However, the low bioavailability of many of the compounds used in these therapeutic strategies continues to limit their clinical application. In order to overcome these limitations, the main objective of this dissertation was to develop a new polymeric formulation in its native and acrylated form for the formation of hydrogels. The incorporation of porphyrin derivatives in these platforms was also promoted through covalent bonding, thus constituting a multifunctional system. To achieve this objective, porphyrin derivatives were prepared and subsequently functionalised with methacrylate derivative, obtaining porphyrin derivatives with different degrees of substitution. Similarly, the polymer matrix was functionalised and the conjugation between acrylated and non-acrylated polymer matrices with porphyrin derivatives was promoted, obtaining polymer matrices conjugated with porphyrins. All porphyrin derivatives and polymer conjugates prepared were subjected to structural characterisation by ¹H, ¹³C and ¹⁹F NMR and, whenever possible, by mass spectrometry and elemental analysis, thus confirming the structures obtained and the incorporation/conjugation of porphyrin into the polymer matrix. Finally, several hydrogel formulations were prepared based on the polymer matrices conjugated with porphyrin in order to evaluate the behaviour of the hydrogel and the influence of the composition on the photoactive response and properties of the material. Analysis of |
| dirty | 0 |
| eu_rights_str_mv | embargoedAccess |
| format | masterThesis |
| id | ria_862a29c00a39da7df26c8ecddff622d9 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10773/46607 |
| instacron_str | ua |
| institution | Universidade de Aveiro |
| instname_str | Universidade de Aveiro |
| language | eng |
| network_acronym_str | ria |
| network_name_str | RIA - Repositório Institucional da Universidade de Aveiro |
| oai_identifier_str | oai:ria.ua.pt:10773/46607 |
| organization_str_mv | urn:organizationAcronym:ua |
| person_str_mv | Olival, Telma Bregieiro |
| publishDate | 2025 |
| reponame_str | RIA - Repositório Institucional da Universidade de Aveiro |
| repository_id_str | urn:repositoryAcronym:ria |
| service_str_mv | urn:repositoryAcronym:ria |
| spelling | pt_PTTargeted therapies for tumour treatment have emerged as promising approaches for inducing effective anti-tumour responses while reducing side effects in healthy tissues. However, the low bioavailability of many of the compounds used in these therapeutic strategies continues to limit their clinical application. In order to overcome these limitations, the main objective of this dissertation was to develop a new polymeric formulation in its native and acrylated form for the formation of hydrogels. The incorporation of porphyrin derivatives in these platforms was also promoted through covalent bonding, thus constituting a multifunctional system. To achieve this objective, porphyrin derivatives were prepared and subsequently functionalised with methacrylate derivative, obtaining porphyrin derivatives with different degrees of substitution. Similarly, the polymer matrix was functionalised and the conjugation between acrylated and non-acrylated polymer matrices with porphyrin derivatives was promoted, obtaining polymer matrices conjugated with porphyrins. All porphyrin derivatives and polymer conjugates prepared were subjected to structural characterisation by ¹H, ¹³C and ¹⁹F NMR and, whenever possible, by mass spectrometry and elemental analysis, thus confirming the structures obtained and the incorporation/conjugation of porphyrin into the polymer matrix. Finally, several hydrogel formulations were prepared based on the polymer matrices conjugated with porphyrin in order to evaluate the behaviour of the hydrogel and the influence of the composition on the photoactive response and properties of the material. Analysis ofpt_PTAs terapias direcionadas para o tratamento de tumores têm vindo a afirmar-se como abordagens promissoras para induzir respostas anti-tumorais eficazes, reduzindo os efeitos secundários em tecidos saudáveis. No entanto, a baixa biodisponibilidade de muitos dos compostos utilizados nestas estratégias terapêuticas continua a limitar a sua aplicação clínica. No sentido de colmatar estas limitações, esta dissertação teve como objetivo principal o desenvolvimento de uma nova formulação polimérica na sua forma nativa e acrilatada, para a formação de hidrogéis. Foi também promovida a incorporação de derivados porfirínicos nestas plataformas através de ligação covalente, constituindo assim um sistema multifuncional. Para atingir este objetivo, foram preparados derivados porfirínicos que posteriormente foram funcionalizados com metacrilato, obtendo-se derivados porfirínicos com diferentes graus de substituição. Analogamente, funcionalizou-se a matriz polimérica e promoveu-se a conjugação entre as matrizes poliméricas acrilatadas e não acrilatadas com os derivados porfirínicos, obtendo-se matrizes poliméricas conjugadas com porfirinas. Todos os derivados porfirínicos e conjugados poliméricos preparados foram sujeitos a caracterização estrutural por RMN de ¹H, ¹³C e ¹⁹F e ainda sempre que possível por espetrometria de massa e análise elementar, confirmando desta forma as estruturas obtidas e a incorporação/conjugação da porfirina na matriz polimérica. Finalmente, foram preparadas várias formulações de hidrogéis com base nas matrizes poliméricas conjugadas com porfirina, de modo a avaliar o comportamento do hidrogel e a influência da composição na resposta fotoativa e nas propriedades do material. A análise das propriedades fotoquímicas e fotofísicas dos derivados preparados demonstrou que nem a funcionalização da porfirina nem a sua conjugação à matriz polimérica afetou as propriedades óticas, comprovando a preservação da estrutura eletrónica da porfirina após conjugação. Todos os derivados porfirínicos demonstram ainda manter as propriedades emissivas mesmo quando imobilizadas no hidrogel em estado sólido.application/pdfengpt_PTPhotoresponsive hydrogels as a therapeutic platform for cancer treatmentOlival, Telma BregieiroHandlehttp://hdl.handle.net/10773/466072027-12-10T00:00:00Z2025-12-10T00:00:00Z2025-12-10http://purl.org/coar/access_right/c_f1cfembargoed accesspt_PTHydrogelspt_PTPorphyrinspt_PTPolimeric matricespt_PTTumors4015749 byteshttp://purl.org/coar/access_right/c_f1cfapplication/pdffulltexthttps://ria.ua.pt/bitstream/10773/46607/1/Documento_Telma_Olival.pdfother research producthttp://purl.org/coar/resource_type/c_bdccmaster thesis |
| spellingShingle | Photoresponsive hydrogels as a therapeutic platform for cancer treatment Olival, Telma Bregieiro Hydrogels Porphyrins Polimeric matrices Tumors |
| status | SINGLETON |
| subject.fl_str_mv | Hydrogels Porphyrins Polimeric matrices Tumors |
| title | Photoresponsive hydrogels as a therapeutic platform for cancer treatment |
| title_full | Photoresponsive hydrogels as a therapeutic platform for cancer treatment |
| title_fullStr | Photoresponsive hydrogels as a therapeutic platform for cancer treatment |
| title_full_unstemmed | Photoresponsive hydrogels as a therapeutic platform for cancer treatment |
| title_short | Photoresponsive hydrogels as a therapeutic platform for cancer treatment |
| title_sort | Photoresponsive hydrogels as a therapeutic platform for cancer treatment |
| topic | Hydrogels Porphyrins Polimeric matrices Tumors |
| topic_facet | Hydrogels Porphyrins Polimeric matrices Tumors |
| url | http://hdl.handle.net/10773/46607 |
| visible | 1 |