Publication
Proteomic analysis of tissue-specific protein aggregation signatures throughout mammalian aging
| Summary: | Proteome and proteostasis network ( disruptions lead to proteostasis imbalances and accumulation of protein aggregates, characteristic of several age related diseases 1 3 The consequences of PN disturbances in the context of healthy aging remains to be thoroughly studied in mammals To determine whether accumulation of insoluble proteins occurs during mammalian aging, young adult 6 month old), middle aged 13 month old), and old 18 and 24 month old) C 57 BL/ 6 mice were used to obtain detergent insoluble fractions Detergent insoluble and soluble fractions were isolated from total protein extracts of the liver, followed by characterization of total, detergent soluble, and detergent insoluble protein profiles We performed SWATH mass spectrometry analysis to identify differential proteome signatures of aging and determine which proteins are more prone to aggregate and further elucidated the altered functions and biological processes. |
|---|---|
| Main Authors: | Francisco, Stephany |
| Other Authors: | Nobre, Ana; Ferreira, Margarida; Santa, Cátia; Anjo, Sandra; Manadas, Bruno; Santos, Manuel; Soares, Ana |
| Subject: | Proteostasis Protein aggregation Liver Proteomics, aging |
| Year: | 2020 |
| Country: | Portugal |
| Document type: | conference output |
| Access type: | restricted access |
| Associated institution: | Universidade de Aveiro |
| Language: | English |
| Origin: | RIA - Repositório Institucional da Universidade de Aveiro |
| _version_ | 1866172988760522752 |
|---|---|
| author | Francisco, Stephany |
| author2 | Nobre, Ana Ferreira, Margarida Santa, Cátia Anjo, Sandra Manadas, Bruno Santos, Manuel Soares, Ana |
| author2_role | author author author author author author author |
| author_facet | Francisco, Stephany Nobre, Ana Ferreira, Margarida Santa, Cátia Anjo, Sandra Manadas, Bruno Santos, Manuel Soares, Ana |
| author_role | author |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Francisco, Stephany\"},{\"Person.name\":\"Nobre, Ana\"},{\"Person.name\":\"Ferreira, Margarida\"},{\"Person.name\":\"Santa, Cátia\"},{\"Person.name\":\"Anjo, Sandra\"},{\"Person.name\":\"Manadas, Bruno\"},{\"Person.name\":\"Santos, Manuel\"},{\"Person.name\":\"Soares, Ana\"}] |
| datacite.creators.creator.creatorName.fl_str_mv | Francisco, Stephany Nobre, Ana Ferreira, Margarida Santa, Cátia Anjo, Sandra Manadas, Bruno Santos, Manuel Soares, Ana |
| datacite.date.Accepted.fl_str_mv | 2020-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2025-06-13T10:45:01Z |
| datacite.date.embargoed.fl_str_mv | 2025-06-13T10:45:01Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Proteostasis Protein aggregation Liver Proteomics, aging |
| datacite.titles.title.fl_str_mv | Proteomic analysis of tissue-specific protein aggregation signatures throughout mammalian aging |
| dc.creator.none.fl_str_mv | Francisco, Stephany Nobre, Ana Ferreira, Margarida Santa, Cátia Anjo, Sandra Manadas, Bruno Santos, Manuel Soares, Ana |
| dc.date.Accepted.fl_str_mv | 2020-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2025-06-13T10:45:01Z |
| dc.date.embargoed.fl_str_mv | 2025-06-13T10:45:01Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10773/31537 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Proteostasis Protein aggregation Liver Proteomics, aging |
| dc.title.fl_str_mv | Proteomic analysis of tissue-specific protein aggregation signatures throughout mammalian aging |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Proteome and proteostasis network ( disruptions lead to proteostasis imbalances and accumulation of protein aggregates, characteristic of several age related diseases 1 3 The consequences of PN disturbances in the context of healthy aging remains to be thoroughly studied in mammals To determine whether accumulation of insoluble proteins occurs during mammalian aging, young adult 6 month old), middle aged 13 month old), and old 18 and 24 month old) C 57 BL/ 6 mice were used to obtain detergent insoluble fractions Detergent insoluble and soluble fractions were isolated from total protein extracts of the liver, followed by characterization of total, detergent soluble, and detergent insoluble protein profiles We performed SWATH mass spectrometry analysis to identify differential proteome signatures of aging and determine which proteins are more prone to aggregate and further elucidated the altered functions and biological processes. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | conferenceObject |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10773/31537 |
| instacron_str | ua |
| institution | Universidade de Aveiro |
| instname_str | Universidade de Aveiro |
| language | eng |
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| network_name_str | RIA - Repositório Institucional da Universidade de Aveiro |
| oai_identifier_str | oai:ria.ua.pt:10773/31537 |
| organization_str_mv | urn:organizationAcronym:ua |
| person_str_mv | Francisco, Stephany Nobre, Ana Ferreira, Margarida Santa, Cátia Anjo, Sandra Manadas, Bruno Santos, Manuel Soares, Ana |
| publishDate | 2020 |
| reponame_str | RIA - Repositório Institucional da Universidade de Aveiro |
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| service_str_mv | urn:repositoryAcronym:ria |
| spelling | pt_PTProteome and proteostasis network ( disruptions lead to proteostasis imbalances and accumulation of protein aggregates, characteristic of several age related diseases 1 3 The consequences of PN disturbances in the context of healthy aging remains to be thoroughly studied in mammals To determine whether accumulation of insoluble proteins occurs during mammalian aging, young adult 6 month old), middle aged 13 month old), and old 18 and 24 month old) C 57 BL/ 6 mice were used to obtain detergent insoluble fractions Detergent insoluble and soluble fractions were isolated from total protein extracts of the liver, followed by characterization of total, detergent soluble, and detergent insoluble protein profiles We performed SWATH mass spectrometry analysis to identify differential proteome signatures of aging and determine which proteins are more prone to aggregate and further elucidated the altered functions and biological processes.application/pdfengpt_PTProteomic analysis of tissue-specific protein aggregation signatures throughout mammalian agingFrancisco, StephanyNobre, AnaFerreira, MargaridaSanta, CátiaAnjo, SandraManadas, BrunoSantos, ManuelSoares, AnaHandlehttp://hdl.handle.net/10773/315372025-06-13T10:45:01Z2020-01-01T00:00:00Z2020http://purl.org/coar/access_right/c_16ecrestricted accesspt_PTProteostasispt_PTProtein aggregationpt_PTLiverpt_PTProteomics, aging719065 byteshttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://ria.ua.pt/bitstream/10773/31537/1/StephanyFrancisco_Proteomic%20analysis%20of%20tissue-specific%20protein%20aggregation%20signatures_CSHLPoster_2020.pdfother research producthttp://purl.org/coar/resource_type/c_c94fconference object |
| spellingShingle | Proteomic analysis of tissue-specific protein aggregation signatures throughout mammalian aging Francisco, Stephany Proteostasis Protein aggregation Liver Proteomics, aging |
| status | SINGLETON |
| subject.fl_str_mv | Proteostasis Protein aggregation Liver Proteomics, aging |
| title | Proteomic analysis of tissue-specific protein aggregation signatures throughout mammalian aging |
| title_full | Proteomic analysis of tissue-specific protein aggregation signatures throughout mammalian aging |
| title_fullStr | Proteomic analysis of tissue-specific protein aggregation signatures throughout mammalian aging |
| title_full_unstemmed | Proteomic analysis of tissue-specific protein aggregation signatures throughout mammalian aging |
| title_short | Proteomic analysis of tissue-specific protein aggregation signatures throughout mammalian aging |
| title_sort | Proteomic analysis of tissue-specific protein aggregation signatures throughout mammalian aging |
| topic | Proteostasis Protein aggregation Liver Proteomics, aging |
| topic_facet | Proteostasis Protein aggregation Liver Proteomics, aging |
| url | http://hdl.handle.net/10773/31537 |
| visible | 1 |