Publicação
Differentially Expressed miRNAs and Functional Pathways Changes in Human Alveolar Epithelial Cells After 1 and 7 Days Exposure to MWCNT-7 or Asbestos
| Resumo: | Environmental and occupational exposure to multi-walled carbon nanotubes (MWCNT), an engineered nanomaterial widely used in industrial and biomedical applications, has been associated with pulmonary adverse effects upon inhalation. Particularly, MWCNT-7 (Mitsui-7) has been classified as possibly carcinogenic to humans, and many toxicological studies have compared it with asbestos due to their similarity in physical characteristics and biopersistence, and the well-known pulmonary adverse effects of asbestos. Several pulmonary diseases have been associated with miRNA expression changes, thus, in this study, we compare the differentially expressed miRNAs (DEmiRNAs) and enriched KEGG pathways after 1 and 7 days exposure to MWCNT-7 or crocidolite, in order to understand the mechanisms of biological response to these materials over time and their possible similarity. For this purpose, miRNAs were sequenced in exposed and non-exposed A549 cells at both time points by next-generation sequencing, and the enriched cellular pathways identified as previously described with minor modifications1. A higher number of DEmiRNAs was observed at 24 h compared to 7 days (17 vs. 3 and 21 vs. 10 for MWCNT-7 and crocidolite, respectively). Accordingly, the number of enriched pathways was also higher at 24 h (23 vs. 30 at 24 h and 7 vs. 5 at 7 days for MWCNT-7 and crocidolite, respectively). At 7 days, most altered pathways were related to cancer, highlighting the carcinogenic potential of MWCNT-7 and crocidolite. The reduction of the number of affected functional pathways likely reflects a greatest impact of these materials shortly after exposure and/or that the differences in DEmiRNAs between non-exposed and exposed cells are less significant after long-term culture. 1Ventura et al, Toxicology Letters 328: 7-18 |
|---|---|
| Autores principais: | Ventura, Célia |
| Outros Autores: | Vieira, Luís; Silva, Catarina; Silva, Maria João |
| Assunto: | Nanomaterials Environmental Genotoxicity miRNA Multiwalled carbon nanotubes Nanotoxicology Genotoxicidade Ambiental Asbestos |
| Ano: | 2020 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Instituto Nacional de Saúde Doutor Ricardo Jorge |
| Idioma: | inglês |
| Origem: | Repositório Científico do Instituto Nacional de Saúde |
| _version_ | 1865920413722214400 |
|---|---|
| author | Ventura, Célia |
| author2 | Vieira, Luís Silva, Catarina Silva, Maria João |
| author2_role | author author author |
| author_facet | Ventura, Célia Ventura, Célia Vieira, Luís Silva, Catarina Silva, Maria João Vieira, Luís Silva, Catarina Silva, Maria João |
| author_role | author |
| contributor_name_str_mv | Repositório Científico do Instituto Nacional de Saúde |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Ventura, Célia\",\"Person.identifier.orcid\":\"0000-0002-0637-2222\"},{\"Person.name\":\"Vieira, Luís\"},{\"Person.name\":\"Silva, Catarina\"},{\"Person.name\":\"Silva, Maria João\",\"Person.identifier.orcid\":\"0000-0002-6060-0716\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico do Instituto Nacional de Saúde |
| datacite.creators.creator.creatorName.fl_str_mv | Ventura, Célia Vieira, Luís Silva, Catarina Silva, Maria João |
| datacite.date.Accepted.fl_str_mv | 2020-09-12T00:00:00Z |
| datacite.date.available.fl_str_mv | 2021-03-13T15:45:30Z |
| datacite.date.embargoed.fl_str_mv | 2021-03-13T15:45:30Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Nanomaterials Environmental Genotoxicity miRNA Multiwalled carbon nanotubes Nanotoxicology Genotoxicidade Ambiental Asbestos |
| datacite.titles.title.fl_str_mv | Differentially Expressed miRNAs and Functional Pathways Changes in Human Alveolar Epithelial Cells After 1 and 7 Days Exposure to MWCNT-7 or Asbestos |
| dc.contributor.none.fl_str_mv | Repositório Científico do Instituto Nacional de Saúde |
| dc.creator.none.fl_str_mv | Ventura, Célia Vieira, Luís Silva, Catarina Silva, Maria João |
| dc.date.Accepted.fl_str_mv | 2020-09-12T00:00:00Z |
| dc.date.available.fl_str_mv | 2021-03-13T15:45:30Z |
| dc.date.embargoed.fl_str_mv | 2021-03-13T15:45:30Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.18/7450 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Nanomaterials Environmental Genotoxicity miRNA Multiwalled carbon nanotubes Nanotoxicology Genotoxicidade Ambiental Asbestos |
| dc.title.fl_str_mv | Differentially Expressed miRNAs and Functional Pathways Changes in Human Alveolar Epithelial Cells After 1 and 7 Days Exposure to MWCNT-7 or Asbestos |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Environmental and occupational exposure to multi-walled carbon nanotubes (MWCNT), an engineered nanomaterial widely used in industrial and biomedical applications, has been associated with pulmonary adverse effects upon inhalation. Particularly, MWCNT-7 (Mitsui-7) has been classified as possibly carcinogenic to humans, and many toxicological studies have compared it with asbestos due to their similarity in physical characteristics and biopersistence, and the well-known pulmonary adverse effects of asbestos. Several pulmonary diseases have been associated with miRNA expression changes, thus, in this study, we compare the differentially expressed miRNAs (DEmiRNAs) and enriched KEGG pathways after 1 and 7 days exposure to MWCNT-7 or crocidolite, in order to understand the mechanisms of biological response to these materials over time and their possible similarity. For this purpose, miRNAs were sequenced in exposed and non-exposed A549 cells at both time points by next-generation sequencing, and the enriched cellular pathways identified as previously described with minor modifications1. A higher number of DEmiRNAs was observed at 24 h compared to 7 days (17 vs. 3 and 21 vs. 10 for MWCNT-7 and crocidolite, respectively). Accordingly, the number of enriched pathways was also higher at 24 h (23 vs. 30 at 24 h and 7 vs. 5 at 7 days for MWCNT-7 and crocidolite, respectively). At 7 days, most altered pathways were related to cancer, highlighting the carcinogenic potential of MWCNT-7 and crocidolite. The reduction of the number of affected functional pathways likely reflects a greatest impact of these materials shortly after exposure and/or that the differences in DEmiRNAs between non-exposed and exposed cells are less significant after long-term culture. 1Ventura et al, Toxicology Letters 328: 7-18 |
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| funding.funder.alternateName_str_mv | FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 3599-PPCDT 6817 - DCRRNI ID |
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| institution | Instituto Nacional de Saúde Doutor Ricardo Jorge |
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| language | eng |
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| organization_str_mv | urn:organizationAcronym:rinsa |
| person_str_mv | Ventura, Célia Ventura, Célia https://www.ciencia-id.pt/6116-89BA-C617 6116-89BA-C617 http://orcid.org/0000-0002-0637-2222 0000-0002-0637-2222 Vieira, Luís Silva, Catarina Silva, Maria João Silva, Maria João https://www.ciencia-id.pt/7710-643D-97A3 7710-643D-97A3 http://orcid.org/0000-0002-6060-0716 0000-0002-6060-0716 |
| publishDate | 2020 |
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| spelling | engpt_PTEnvironmental and occupational exposure to multi-walled carbon nanotubes (MWCNT), an engineered nanomaterial widely used in industrial and biomedical applications, has been associated with pulmonary adverse effects upon inhalation. Particularly, MWCNT-7 (Mitsui-7) has been classified as possibly carcinogenic to humans, and many toxicological studies have compared it with asbestos due to their similarity in physical characteristics and biopersistence, and the well-known pulmonary adverse effects of asbestos. Several pulmonary diseases have been associated with miRNA expression changes, thus, in this study, we compare the differentially expressed miRNAs (DEmiRNAs) and enriched KEGG pathways after 1 and 7 days exposure to MWCNT-7 or crocidolite, in order to understand the mechanisms of biological response to these materials over time and their possible similarity. For this purpose, miRNAs were sequenced in exposed and non-exposed A549 cells at both time points by next-generation sequencing, and the enriched cellular pathways identified as previously described with minor modifications1. A higher number of DEmiRNAs was observed at 24 h compared to 7 days (17 vs. 3 and 21 vs. 10 for MWCNT-7 and crocidolite, respectively). Accordingly, the number of enriched pathways was also higher at 24 h (23 vs. 30 at 24 h and 7 vs. 5 at 7 days for MWCNT-7 and crocidolite, respectively). At 7 days, most altered pathways were related to cancer, highlighting the carcinogenic potential of MWCNT-7 and crocidolite. The reduction of the number of affected functional pathways likely reflects a greatest impact of these materials shortly after exposure and/or that the differences in DEmiRNAs between non-exposed and exposed cells are less significant after long-term culture. 1Ventura et al, Toxicology Letters 328: 7-18application/pdfpt_PTDifferentially Expressed miRNAs and Functional Pathways Changes in Human Alveolar Epithelial Cells After 1 and 7 Days Exposure to MWCNT-7 or AsbestosPersonalVentura, CéliaDSpacehttp://dspace.org/items/fa89f42b-12a4-47bd-a4fb-0f65cbc06885DSpacehttp://dspace.org/items/fa89f42b-12a4-47bd-a4fb-0f65cbc06885VenturaCéliaCiência IDhttps://www.ciencia-id.pt6116-89BA-C617ORCIDhttp://orcid.org0000-0002-0637-2222Vieira, LuísSilva, CatarinaPersonalSilva, Maria JoãoDSpacehttp://dspace.org/items/a7763685-7c34-468d-b958-9dd0aca66db4DSpacehttp://dspace.org/items/a7763685-7c34-468d-b958-9dd0aca66db4SilvaMaria JoaoCiência IDhttps://www.ciencia-id.pt7710-643D-97A3ORCIDhttp://orcid.org0000-0002-6060-0716Scopus Author IDhttps://www.scopus.com55944437600HostingInstitutionOrganizationalRepositório Científico do Instituto Nacional de Saúdee-mailmailto:repositorio.cientifico@insa.min-saude.ptrepositorio.cientifico@insa.min-saude.pt2021-03-13T15:45:30Z2020-09-122020-09-12T00:00:00ZHandlehttp://hdl.handle.net/10400.18/7450http://purl.org/coar/access_right/c_16ecrestricted accessNanomaterialsEnvironmental GenotoxicitymiRNAMultiwalled carbon nanotubesNanotoxicologyGenotoxicidade AmbientalAsbestos1516294 bytesFundação para a Ciência e a TecnologiaA predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system3599-PPCDTCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaCentre for Toxicogenomics and Human Health6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871other research producthttp://purl.org/coar/resource_type/c_c94fconference objecthttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.insa.pt/bitstreams/00f4dad9-1340-492e-a7fb-abc96a7f65dc/downloadEnvironmental Mutagenesis & Genomics Society Virtual Annual Meeting (EMGS), 12-16 September 2020(online) |
| spellingShingle | Differentially Expressed miRNAs and Functional Pathways Changes in Human Alveolar Epithelial Cells After 1 and 7 Days Exposure to MWCNT-7 or Asbestos Differentially Expressed miRNAs and Functional Pathways Changes in Human Alveolar Epithelial Cells After 1 and 7 Days Exposure to MWCNT-7 or Asbestos Ventura, Célia Nanomaterials Environmental Genotoxicity miRNA Multiwalled carbon nanotubes Nanotoxicology Genotoxicidade Ambiental Asbestos Ventura, Célia Nanomaterials Environmental Genotoxicity miRNA Multiwalled carbon nanotubes Nanotoxicology Genotoxicidade Ambiental Asbestos |
| status | NEW |
| subject.fl_str_mv | Nanomaterials Environmental Genotoxicity miRNA Multiwalled carbon nanotubes Nanotoxicology Genotoxicidade Ambiental Asbestos |
| title | Differentially Expressed miRNAs and Functional Pathways Changes in Human Alveolar Epithelial Cells After 1 and 7 Days Exposure to MWCNT-7 or Asbestos |
| title_full | Differentially Expressed miRNAs and Functional Pathways Changes in Human Alveolar Epithelial Cells After 1 and 7 Days Exposure to MWCNT-7 or Asbestos |
| title_fullStr | Differentially Expressed miRNAs and Functional Pathways Changes in Human Alveolar Epithelial Cells After 1 and 7 Days Exposure to MWCNT-7 or Asbestos Differentially Expressed miRNAs and Functional Pathways Changes in Human Alveolar Epithelial Cells After 1 and 7 Days Exposure to MWCNT-7 or Asbestos |
| title_full_unstemmed | Differentially Expressed miRNAs and Functional Pathways Changes in Human Alveolar Epithelial Cells After 1 and 7 Days Exposure to MWCNT-7 or Asbestos Differentially Expressed miRNAs and Functional Pathways Changes in Human Alveolar Epithelial Cells After 1 and 7 Days Exposure to MWCNT-7 or Asbestos |
| title_short | Differentially Expressed miRNAs and Functional Pathways Changes in Human Alveolar Epithelial Cells After 1 and 7 Days Exposure to MWCNT-7 or Asbestos |
| title_sort | Differentially Expressed miRNAs and Functional Pathways Changes in Human Alveolar Epithelial Cells After 1 and 7 Days Exposure to MWCNT-7 or Asbestos |
| topic | Nanomaterials Environmental Genotoxicity miRNA Multiwalled carbon nanotubes Nanotoxicology Genotoxicidade Ambiental Asbestos |
| topic_facet | Nanomaterials Environmental Genotoxicity miRNA Multiwalled carbon nanotubes Nanotoxicology Genotoxicidade Ambiental Asbestos |
| url | http://hdl.handle.net/10400.18/7450 |
| visible | 1 |