Publicação
Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype
| Resumo: | Euphorbia heterophylla is a weed species that invades extensive crop areas in subtropical regions of Brazil. This species was previously controlled by imazamox, but the continuous use of this herbicide has selected for resistant biotypes. Two biotypes of E. heterophylla from southern Brazil, one resistant (R) and one susceptible (S) to imazamox, were compared. The resistance of the R biotype was confrmed by dose-response assays since it required 1250.2g ai ha−1 to reduce the fresh weight by 50% versus 7.4g ai ha−1 for the S biotype. The acetolactate synthase (ALS) enzyme activity was studied using ALS-inhibiting herbicides from fve diferent chemical families. The R biotype required the highest concentrations to reduce this enzyme activity by 50%. A Ser653Asn mutation was found in the ALS gene of the R biotype. The experiments carried out showed that imazamox absorption and metabolism were not involved in resistance. However, greater 14C-imazamox root exudation was found in the R biotype (~70% of the total absorbed imazamox). Target site mutation in the ALS gene is the principal mechanism that explains the imazamox resistance of the R biotype, but root exudation seems to also contribute to the resistance of this biotype |
|---|---|
| Autores principais: | Rojano-Blanco, Antonia |
| Outros Autores: | Palma-Bautista, Candelario; Alcantara-de la Cruz, Ricardo; Torra, Joel; Alcantara, Esteban; De Prado, Rafael; Fernandes, João |
| Assunto: | Euphorbia heterophylla Pest control Extensive crop areas Imazamox Controlo de infestantes Herbicida |
| Ano: | 2019 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Beja |
| Idioma: | inglês |
| Origem: | Repositório Institucional do IPBeja |
| _version_ | 1869070476904497152 |
|---|---|
| author | Rojano-Blanco, Antonia |
| author2 | Palma-Bautista, Candelario Alcantara-de la Cruz, Ricardo Torra, Joel Alcantara, Esteban De Prado, Rafael Fernandes, João |
| author2_role | author author author author author author |
| author_facet | Rojano-Blanco, Antonia Palma-Bautista, Candelario Alcantara-de la Cruz, Ricardo Torra, Joel Alcantara, Esteban De Prado, Rafael Fernandes, João |
| author_role | author |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Rojano-Blanco, Antonia\"},{\"Person.name\":\"Palma-Bautista, Candelario\"},{\"Person.name\":\"Alcantara-de la Cruz, Ricardo\"},{\"Person.name\":\"Torra, Joel\"},{\"Person.name\":\"Alcantara, Esteban\"},{\"Person.name\":\"De Prado, Rafael\"},{\"Person.name\":\"Fernandes, João\"}] |
| datacite.creators.creator.creatorName.fl_str_mv | Rojano-Blanco, Antonia Palma-Bautista, Candelario Alcantara-de la Cruz, Ricardo Torra, Joel Alcantara, Esteban De Prado, Rafael Fernandes, João |
| datacite.date.Accepted.fl_str_mv | 2019-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2022-05-05T12:12:40Z |
| datacite.date.embargoed.fl_str_mv | 2022-05-05T12:12:40Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Euphorbia heterophylla Pest control Extensive crop areas Imazamox Controlo de infestantes Herbicida |
| datacite.titles.title.fl_str_mv | Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype |
| dc.creator.none.fl_str_mv | Rojano-Blanco, Antonia Palma-Bautista, Candelario Alcantara-de la Cruz, Ricardo Torra, Joel Alcantara, Esteban De Prado, Rafael Fernandes, João |
| dc.date.Accepted.fl_str_mv | 2019-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2022-05-05T12:12:40Z |
| dc.date.embargoed.fl_str_mv | 2022-05-05T12:12:40Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/20.500.12207/5424 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Nature |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Euphorbia heterophylla Pest control Extensive crop areas Imazamox Controlo de infestantes Herbicida |
| dc.title.fl_str_mv | Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Euphorbia heterophylla is a weed species that invades extensive crop areas in subtropical regions of Brazil. This species was previously controlled by imazamox, but the continuous use of this herbicide has selected for resistant biotypes. Two biotypes of E. heterophylla from southern Brazil, one resistant (R) and one susceptible (S) to imazamox, were compared. The resistance of the R biotype was confrmed by dose-response assays since it required 1250.2g ai ha−1 to reduce the fresh weight by 50% versus 7.4g ai ha−1 for the S biotype. The acetolactate synthase (ALS) enzyme activity was studied using ALS-inhibiting herbicides from fve diferent chemical families. The R biotype required the highest concentrations to reduce this enzyme activity by 50%. A Ser653Asn mutation was found in the ALS gene of the R biotype. The experiments carried out showed that imazamox absorption and metabolism were not involved in resistance. However, greater 14C-imazamox root exudation was found in the R biotype (~70% of the total absorbed imazamox). Target site mutation in the ALS gene is the principal mechanism that explains the imazamox resistance of the R biotype, but root exudation seems to also contribute to the resistance of this biotype |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ripb_eb40c9d8fa33df588514192587ce8a34 |
| identifier.url.fl_str_mv | http://hdl.handle.net/20.500.12207/5424 |
| inst_facet_str | urn:organizationAcronym:ipb{{{_:::_}}}Instituto Politécnico de Beja |
| instacron_str | ipb |
| institution | Instituto Politécnico de Beja |
| instname_str | Instituto Politécnico de Beja |
| language | eng |
| network_acronym_str | ripb |
| network_name_str | Repositório Institucional do IPBeja |
| oai_identifier_str | oai:repositorio.ipbeja.pt:20.500.12207/5424 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Rojano-Blanco, Antonia Palma-Bautista, Candelario Alcantara-de la Cruz, Ricardo Torra, Joel Alcantara, Esteban De Prado, Rafael Fernandes, João |
| publishDate | 2019 |
| publisher.none.fl_str_mv | Nature |
| repo_facet_str | urn:repositoryAcronym:ripb{{{_:::_}}}Repositório Institucional do IPBeja |
| reponame_str | Repositório Institucional do IPBeja |
| repository_id_str | urn:repositoryAcronym:ripb |
| service_str_mv | urn:repositoryAcronym:ripb |
| spelling | porEuphorbia heterophylla is a weed species that invades extensive crop areas in subtropical regions of Brazil. This species was previously controlled by imazamox, but the continuous use of this herbicide has selected for resistant biotypes. Two biotypes of E. heterophylla from southern Brazil, one resistant (R) and one susceptible (S) to imazamox, were compared. The resistance of the R biotype was confrmed by dose-response assays since it required 1250.2g ai ha−1 to reduce the fresh weight by 50% versus 7.4g ai ha−1 for the S biotype. The acetolactate synthase (ALS) enzyme activity was studied using ALS-inhibiting herbicides from fve diferent chemical families. The R biotype required the highest concentrations to reduce this enzyme activity by 50%. A Ser653Asn mutation was found in the ALS gene of the R biotype. The experiments carried out showed that imazamox absorption and metabolism were not involved in resistance. However, greater 14C-imazamox root exudation was found in the R biotype (~70% of the total absorbed imazamox). Target site mutation in the ALS gene is the principal mechanism that explains the imazamox resistance of the R biotype, but root exudation seems to also contribute to the resistance of this biotypeapplication/pdfengNatureporTarget site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotypeRojano-Blanco, AntoniaPalma-Bautista, CandelarioAlcantara-de la Cruz, RicardoTorra, JoelAlcantara, EstebanDe Prado, RafaelFernandes, JoãoHandlehttp://hdl.handle.net/20.500.12207/5424DOIIsPartOfhttps://doi.org/10.1038/s41598-019-51682-z2022-05-05T12:12:40Z2019-01-01T00:00:00Z2019http://purl.org/coar/access_right/c_abf2open accessporEuphorbia heterophyllaporPest controlporExtensive crop areasporImazamoxporControlo de infestantesporHerbicida1632874 byteshttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ipbeja.pt/bitstreams/44780af2-3b64-4dc7-a3bd-1ecf9cba6984/downloadliteraturehttp://purl.org/coar/resource_type/c_6501journal article |
| spellingShingle | Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype Rojano-Blanco, Antonia Euphorbia heterophylla Pest control Extensive crop areas Imazamox Controlo de infestantes Herbicida |
| status | SINGLETON |
| subject.fl_str_mv | Euphorbia heterophylla Pest control Extensive crop areas Imazamox Controlo de infestantes Herbicida |
| title | Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype |
| title_full | Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype |
| title_fullStr | Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype |
| title_full_unstemmed | Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype |
| title_short | Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype |
| title_sort | Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype |
| topic | Euphorbia heterophylla Pest control Extensive crop areas Imazamox Controlo de infestantes Herbicida |
| topic_facet | Euphorbia heterophylla Pest control Extensive crop areas Imazamox Controlo de infestantes Herbicida |
| url | http://hdl.handle.net/20.500.12207/5424 |
| visible | 1 |