Publication
Cistus ladanifer L. tissue culture from leaf and stem explants.
| Summary: | Cistus ladanifer L. exudes a phenolic and terpenoid resin with interesting bioactive and aromatic properties. Despite its high abundance in the wild, this plant can be cultivated to advantage on oligotrophic and trace-elements contaminated soils. Plant tissue culture may be used to produce specific metabolites or for clonal propagation of specific genotypes for plantation. From a biotechnological perspective this is the second study that has attempted in vitro propagation of C. ladanifer from adult plant material. Its goal was to evaluate the potential of leaf and internodal stem explants from C. ladanifer for in vitro tissue culture. Three plant growth regulators were tested: 2,4-Dichlorophenoxyacetic acid (2,4-D), 6-Benzylaminopurine (BAP), and 1-Naphthaleneacetic acid (NAA). From both explants, shoots were regenerated under the influence of BAP (38%) and two types of compact calli were induced: dark green calli were induced under the influence of BAP (above 70%) and light green calli were induced under the influence of 2,4-D with or without BAP (100%). Light green calli grew between 558 and 708% during subsequent subcultures and showed rhizogenic capacity when the amounts of BAP were lower than of 2.4-D, but they showed low potential for shoot organogenesis. Dark green calli were associated with shoot organogenesis. The suitability of the two calli lines to produce metabolites and their transposition to liquid cultures is worth further study in comparison to organ in vitro cultures. |
|---|---|
| Main Authors: | Frazão, David F. |
| Other Authors: | Delgado, F.M.G.; Gonçalves, José Carlos; Barroca, Celina; Silva, Amélia M. |
| Subject: | Callogenesis Callus histology Cistaceae In vitro propagation Organogenesis Rockrose |
| Year: | 2022 |
| Country: | Portugal |
| Document type: | article |
| Access type: | open access |
| Associated institution: | Instituto Politécnico de Castelo Branco |
| Language: | English |
| Origin: | Repositório Científico do Instituto Politécnico de Castelo Branco |
| _version_ | 1868350527462440960 |
|---|---|
| author | Frazão, David F. |
| author2 | Delgado, F.M.G. Gonçalves, José Carlos Barroca, Celina Silva, Amélia M. |
| author2_role | author author author author |
| author_facet | Frazão, David F. Delgado, F.M.G. Gonçalves, José Carlos Barroca, Celina Silva, Amélia M. |
| author_role | author |
| contributor_name_str_mv | Repositório Científico do Instituto Politécnico de Castelo Branco |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Frazão, David F.\",\"Person.identifier.orcid\":\"0000-0001-5017-6117\"},{\"Person.name\":\"Delgado, F.M.G.\",\"Person.identifier.orcid\":\"0000-0003-1238-9386\"},{\"Person.name\":\"Gonçalves, José Carlos\",\"Person.identifier.orcid\":\"0000-0003-3387-4487\"},{\"Person.name\":\"Barroca, Celina\"},{\"Person.name\":\"Silva, Amélia M.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico do Instituto Politécnico de Castelo Branco |
| datacite.creators.creator.creatorName.fl_str_mv | Frazão, David F. Delgado, F.M.G. Gonçalves, José Carlos Barroca, Celina Silva, Amélia M. |
| datacite.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2022-12-09T15:01:55Z |
| datacite.date.embargoed.fl_str_mv | 2022-12-09T15:01:55Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Callogenesis Callus histology Cistaceae In vitro propagation Organogenesis Rockrose |
| datacite.titles.title.fl_str_mv | Cistus ladanifer L. tissue culture from leaf and stem explants. |
| dc.contributor.none.fl_str_mv | Repositório Científico do Instituto Politécnico de Castelo Branco |
| dc.creator.none.fl_str_mv | Frazão, David F. Delgado, F.M.G. Gonçalves, José Carlos Barroca, Celina Silva, Amélia M. |
| dc.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2022-12-09T15:01:55Z |
| dc.date.embargoed.fl_str_mv | 2022-12-09T15:01:55Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.11/8199 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Callogenesis Callus histology Cistaceae In vitro propagation Organogenesis Rockrose |
| dc.title.fl_str_mv | Cistus ladanifer L. tissue culture from leaf and stem explants. |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Cistus ladanifer L. exudes a phenolic and terpenoid resin with interesting bioactive and aromatic properties. Despite its high abundance in the wild, this plant can be cultivated to advantage on oligotrophic and trace-elements contaminated soils. Plant tissue culture may be used to produce specific metabolites or for clonal propagation of specific genotypes for plantation. From a biotechnological perspective this is the second study that has attempted in vitro propagation of C. ladanifer from adult plant material. Its goal was to evaluate the potential of leaf and internodal stem explants from C. ladanifer for in vitro tissue culture. Three plant growth regulators were tested: 2,4-Dichlorophenoxyacetic acid (2,4-D), 6-Benzylaminopurine (BAP), and 1-Naphthaleneacetic acid (NAA). From both explants, shoots were regenerated under the influence of BAP (38%) and two types of compact calli were induced: dark green calli were induced under the influence of BAP (above 70%) and light green calli were induced under the influence of 2,4-D with or without BAP (100%). Light green calli grew between 558 and 708% during subsequent subcultures and showed rhizogenic capacity when the amounts of BAP were lower than of 2.4-D, but they showed low potential for shoot organogenesis. Dark green calli were associated with shoot organogenesis. The suitability of the two calli lines to produce metabolites and their transposition to liquid cultures is worth further study in comparison to organ in vitro cultures. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ipcb.pt/bitstreams/da43e4b8-f2f4-46b5-adeb-1c0a517b1852/download |
| id | ripcb_0eadbce181f0412b14320e9d9495c8f4 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.11/8199 |
| instacron_str | ipcb |
| institution | Instituto Politécnico de Castelo Branco |
| instname_str | Instituto Politécnico de Castelo Branco |
| language | eng |
| network_acronym_str | ripcb |
| network_name_str | Repositório Científico do Instituto Politécnico de Castelo Branco |
| oai_identifier_str | oai:repositorio.ipcb.pt:10400.11/8199 |
| organization_str_mv | urn:organizationAcronym:ipcb |
| person_str_mv | Frazão, David F. Frazão, David F. https://www.ciencia-id.pt/4913-7383-FB1A 4913-7383-FB1A http://orcid.org/0000-0001-5017-6117 0000-0001-5017-6117 Delgado, F.M.G. Delgado, F.M.G. https://www.ciencia-id.pt/C715-76B6-A07D C715-76B6-A07D http://orcid.org/0000-0003-1238-9386 0000-0003-1238-9386 Gonçalves, José Carlos Gonçalves, José Carlos https://www.ciencia-id.pt/AE1F-12BE-65DD AE1F-12BE-65DD http://orcid.org/0000-0003-3387-4487 0000-0003-3387-4487 Barroca, Celina Silva, Amélia M. |
| publishDate | 2022 |
| reponame_str | Repositório Científico do Instituto Politécnico de Castelo Branco |
| repository_id_str | urn:repositoryAcronym:ripcb |
| service_str_mv | urn:repositoryAcronym:ripcb |
| spelling | engCistus ladanifer L. exudes a phenolic and terpenoid resin with interesting bioactive and aromatic properties. Despite its high abundance in the wild, this plant can be cultivated to advantage on oligotrophic and trace-elements contaminated soils. Plant tissue culture may be used to produce specific metabolites or for clonal propagation of specific genotypes for plantation. From a biotechnological perspective this is the second study that has attempted in vitro propagation of C. ladanifer from adult plant material. Its goal was to evaluate the potential of leaf and internodal stem explants from C. ladanifer for in vitro tissue culture. Three plant growth regulators were tested: 2,4-Dichlorophenoxyacetic acid (2,4-D), 6-Benzylaminopurine (BAP), and 1-Naphthaleneacetic acid (NAA). From both explants, shoots were regenerated under the influence of BAP (38%) and two types of compact calli were induced: dark green calli were induced under the influence of BAP (above 70%) and light green calli were induced under the influence of 2,4-D with or without BAP (100%). Light green calli grew between 558 and 708% during subsequent subcultures and showed rhizogenic capacity when the amounts of BAP were lower than of 2.4-D, but they showed low potential for shoot organogenesis. Dark green calli were associated with shoot organogenesis. The suitability of the two calli lines to produce metabolites and their transposition to liquid cultures is worth further study in comparison to organ in vitro cultures.application/pdfen_USCistus ladanifer L. tissue culture from leaf and stem explants.PersonalFrazão, David F.DSpacehttp://dspace.org/items/4c55356e-318e-4c30-8b07-577d0c3d48cfDSpacehttp://dspace.org/items/4c55356e-318e-4c30-8b07-577d0c3d48cfFranco FrazãoDavidCiência IDhttps://www.ciencia-id.pt4913-7383-FB1AORCIDhttp://orcid.org0000-0001-5017-6117PersonalDelgado, F.M.G.DSpacehttp://dspace.org/items/8ae072f8-0aa1-46ce-830a-210c42f9e11bDSpacehttp://dspace.org/items/8ae072f8-0aa1-46ce-830a-210c42f9e11bDelgadoFernandaCiência IDhttps://www.ciencia-id.ptC715-76B6-A07DORCIDhttp://orcid.org0000-0003-1238-9386PersonalGonçalves, José CarlosDSpacehttp://dspace.org/items/3f401604-ccbb-45f3-8ee1-05063cf3d60aDSpacehttp://dspace.org/items/3f401604-ccbb-45f3-8ee1-05063cf3d60aGonçalvesJoséCiência IDhttps://www.ciencia-id.ptAE1F-12BE-65DDORCIDhttp://orcid.org0000-0003-3387-4487Scopus Author IDhttps://www.scopus.com7103325479Barroca, CelinaSilva, Amélia M.HostingInstitutionOrganizationalRepositório Científico do Instituto Politécnico de Castelo Brancoe-mailmailto:repositorio@ipcb.ptrepositorio@ipcb.ptISSNIsPartOf2643-704X2022-12-09T15:01:55Z20222022-11-30T22:45:24Z2022-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.11/8199http://purl.org/coar/access_right/c_abf2open accessCallogenesisCallus histologyCistaceaeIn vitro propagationOrganogenesisRockrose743994 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ipcb.pt/bitstreams/da43e4b8-f2f4-46b5-adeb-1c0a517b1852/download43544550 |
| spellingShingle | Cistus ladanifer L. tissue culture from leaf and stem explants. Frazão, David F. Callogenesis Callus histology Cistaceae In vitro propagation Organogenesis Rockrose |
| status | SINGLETON |
| subject.fl_str_mv | Callogenesis Callus histology Cistaceae In vitro propagation Organogenesis Rockrose |
| title | Cistus ladanifer L. tissue culture from leaf and stem explants. |
| title_full | Cistus ladanifer L. tissue culture from leaf and stem explants. |
| title_fullStr | Cistus ladanifer L. tissue culture from leaf and stem explants. |
| title_full_unstemmed | Cistus ladanifer L. tissue culture from leaf and stem explants. |
| title_short | Cistus ladanifer L. tissue culture from leaf and stem explants. |
| title_sort | Cistus ladanifer L. tissue culture from leaf and stem explants. |
| topic | Callogenesis Callus histology Cistaceae In vitro propagation Organogenesis Rockrose |
| topic_facet | Callogenesis Callus histology Cistaceae In vitro propagation Organogenesis Rockrose |
| url | http://hdl.handle.net/10400.11/8199 |
| visible | 1 |