Publicação
Isoenzymatic variability in populations of Lupinus from Beira Interior
| Resumo: | Isoenzyrnatic studies were performed in order to study the varia¬bility of Lupinus spp. populations from Beira Interior region. Thirty accessions of Lupinus (L. luteus, L. albus, L. angustifoius and L. Hispanicus) from Beira interior region were studied. The zymograms were obtained, by poliacrilamide gel ele¬ctrophoresis (PAGE). Extracts were prepared from young leaf and cotyledons tissues. Four enzyme systems were studied: aspartate aminotransferase (AAT), esterase (EST), acid phos¬phatase (ACT) and malate desidrogenase (MDH). Isoenzyme polymorphisms were found for all the enzyme sys¬tems studied. The comparative analysis of AAT and MDH zymograms show imerspecific variation and made it possible to identify the four species studied. Significant intraspecific varia¬tion was found in EST and ACP zymograms; however intrapopu¬lational variation was also detected and the different popula¬tions could not be distinguished inside each species. The existance of a greater genetic proximity between L. luteus and L. hispanicus was detected. |
|---|---|
| Autores principais: | Frederico, A.M. |
| Outros Autores: | Reis, C.M.G. |
| Assunto: | Lupinus luteus Isoenzyme polimorphism Lupinus hispanicus |
| Ano: | 2002 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Castelo Branco |
| Idioma: | inglês |
| Origem: | Repositório Científico do Instituto Politécnico de Castelo Branco |
| _version_ | 1868350444348112896 |
|---|---|
| author | Frederico, A.M. |
| author2 | Reis, C.M.G. |
| author2_role | author |
| author_facet | Frederico, A.M. Reis, C.M.G. |
| author_role | author |
| contributor_name_str_mv | Repositório Científico do Instituto Politécnico de Castelo Branco |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Frederico, A.M.\"},{\"Person.name\":\"Reis, C.M.G.\",\"Person.identifier.orcid\":\"0000-0002-4854-5128\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico do Instituto Politécnico de Castelo Branco |
| datacite.creators.creator.creatorName.fl_str_mv | Frederico, A.M. Reis, C.M.G. |
| datacite.date.Accepted.fl_str_mv | 2002-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2011-11-15T11:47:12Z |
| datacite.date.embargoed.fl_str_mv | 2011-11-15T11:47:12Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Lupinus luteus Isoenzyme polimorphism Lupinus hispanicus |
| datacite.titles.title.fl_str_mv | Isoenzymatic variability in populations of Lupinus from Beira Interior |
| dc.contributor.none.fl_str_mv | Repositório Científico do Instituto Politécnico de Castelo Branco |
| dc.creator.none.fl_str_mv | Frederico, A.M. Reis, C.M.G. |
| dc.date.Accepted.fl_str_mv | 2002-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2011-11-15T11:47:12Z |
| dc.date.embargoed.fl_str_mv | 2011-11-15T11:47:12Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.11/942 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | SPB |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Lupinus luteus Isoenzyme polimorphism Lupinus hispanicus |
| dc.title.fl_str_mv | Isoenzymatic variability in populations of Lupinus from Beira Interior |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Isoenzyrnatic studies were performed in order to study the varia¬bility of Lupinus spp. populations from Beira Interior region. Thirty accessions of Lupinus (L. luteus, L. albus, L. angustifoius and L. Hispanicus) from Beira interior region were studied. The zymograms were obtained, by poliacrilamide gel ele¬ctrophoresis (PAGE). Extracts were prepared from young leaf and cotyledons tissues. Four enzyme systems were studied: aspartate aminotransferase (AAT), esterase (EST), acid phos¬phatase (ACT) and malate desidrogenase (MDH). Isoenzyme polymorphisms were found for all the enzyme sys¬tems studied. The comparative analysis of AAT and MDH zymograms show imerspecific variation and made it possible to identify the four species studied. Significant intraspecific varia¬tion was found in EST and ACP zymograms; however intrapopu¬lational variation was also detected and the different popula¬tions could not be distinguished inside each species. The existance of a greater genetic proximity between L. luteus and L. hispanicus was detected. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | conferenceObject |
| fulltext.url.fl_str_mv | https://repositorio.ipcb.pt/bitstreams/1fdb9bd7-9213-4a87-87ce-412395d0f5b4/download |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10400.11/942 |
| instacron_str | ipcb |
| institution | Instituto Politécnico de Castelo Branco |
| instname_str | Instituto Politécnico de Castelo Branco |
| language | eng |
| network_acronym_str | ripcb |
| network_name_str | Repositório Científico do Instituto Politécnico de Castelo Branco |
| oai_identifier_str | oai:repositorio.ipcb.pt:10400.11/942 |
| organization_str_mv | urn:organizationAcronym:ipcb |
| person_str_mv | Frederico, A.M. Reis, C.M.G. Reis, C.M.G. https://www.ciencia-id.pt/A71B-FF9D-83C6 A71B-FF9D-83C6 http://orcid.org/0000-0002-4854-5128 0000-0002-4854-5128 |
| publishDate | 2002 |
| publisher.none.fl_str_mv | SPB |
| reponame_str | Repositório Científico do Instituto Politécnico de Castelo Branco |
| repository_id_str | urn:repositoryAcronym:ripcb |
| service_str_mv | urn:repositoryAcronym:ripcb |
| spelling | engSPBporIsoenzyrnatic studies were performed in order to study the varia¬bility of Lupinus spp. populations from Beira Interior region. Thirty accessions of Lupinus (L. luteus, L. albus, L. angustifoius and L. Hispanicus) from Beira interior region were studied. The zymograms were obtained, by poliacrilamide gel ele¬ctrophoresis (PAGE). Extracts were prepared from young leaf and cotyledons tissues. Four enzyme systems were studied: aspartate aminotransferase (AAT), esterase (EST), acid phos¬phatase (ACT) and malate desidrogenase (MDH). Isoenzyme polymorphisms were found for all the enzyme sys¬tems studied. The comparative analysis of AAT and MDH zymograms show imerspecific variation and made it possible to identify the four species studied. Significant intraspecific varia¬tion was found in EST and ACP zymograms; however intrapopu¬lational variation was also detected and the different popula¬tions could not be distinguished inside each species. The existance of a greater genetic proximity between L. luteus and L. hispanicus was detected.application/pdfporIsoenzymatic variability in populations of Lupinus from Beira InteriorFrederico, A.M.PersonalReis, C.M.G.DSpacehttp://dspace.org/items/74d754d4-3926-497b-a0c4-26e98cbe0c93DSpacehttp://dspace.org/items/74d754d4-3926-497b-a0c4-26e98cbe0c93Gaspar ReisCarlosCiência IDhttps://www.ciencia-id.ptA71B-FF9D-83C6ORCIDhttp://orcid.org0000-0002-4854-5128HostingInstitutionOrganizationalRepositório Científico do Instituto Politécnico de Castelo Brancoe-mailmailto:repositorio@ipcb.ptrepositorio@ipcb.pt2011-11-15T11:47:12Z20022002-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.11/942http://purl.org/coar/access_right/c_abf2open accessLupinus luteusIsoenzyme polimorphismLupinus hispanicus260710 bytesother research producthttp://purl.org/coar/resource_type/c_c94fconference objecthttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ipcb.pt/bitstreams/1fdb9bd7-9213-4a87-87ce-412395d0f5b4/downloadXIII Congresso Nacional de Bioquímica, Lisboa, 5-7 Dez. 2002Lisboa |
| spellingShingle | Isoenzymatic variability in populations of Lupinus from Beira Interior Frederico, A.M. Lupinus luteus Isoenzyme polimorphism Lupinus hispanicus |
| status | SINGLETON |
| subject.fl_str_mv | Lupinus luteus Isoenzyme polimorphism Lupinus hispanicus |
| title | Isoenzymatic variability in populations of Lupinus from Beira Interior |
| title_full | Isoenzymatic variability in populations of Lupinus from Beira Interior |
| title_fullStr | Isoenzymatic variability in populations of Lupinus from Beira Interior |
| title_full_unstemmed | Isoenzymatic variability in populations of Lupinus from Beira Interior |
| title_short | Isoenzymatic variability in populations of Lupinus from Beira Interior |
| title_sort | Isoenzymatic variability in populations of Lupinus from Beira Interior |
| topic | Lupinus luteus Isoenzyme polimorphism Lupinus hispanicus |
| topic_facet | Lupinus luteus Isoenzyme polimorphism Lupinus hispanicus |
| url | http://hdl.handle.net/10400.11/942 |
| visible | 1 |