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Production of simple sugars from olive grove pruning using acid pretreatment and enzymatic hydrolysis
| Resumo: | The purpose of this paper was to optimize the production of simple sugars from olive grove pruning (OGP) using acid pretreatment and enzymatic hydrolysis. This study was based on a model composition corresponding to a 34 orthogonal factorial design and employed the response surface methodology (RSM) to optimize the pretreatment and hydrolysis conditions, aiming to attain maximum glucose, xylose and arabinose extraction from cellulose and hemicellulose of biomass. The pretreatment operating conditions considered for optimization, were temperature (60–180 °C), residence time (30–120 min) and sulphuric acid concentration (0.5–5% w w-1). Enzymatic hydrolysis experiments on solid fraction pretreated with diluted acid were performed at a solid concentration of 5% (w v-1, based on dry weight), using 50 mM citrate buffer pH 4.8 with BSA at a concentration of 60 mg g-1 dry biomass. The reaction mixture was incubated at 50 °C for 174 h on an orbital shaker at 150 rpm. Three commercial enzyme preparations (cellulase complex, b-glucosidase and xylanase) were used in enzymatic saccharification. Total carbohydrate content of the initial biomass was 51.25% (in dry mass), of which glucose was the major constituent with 33.59%. Contents of lignin and extractable found in biomass were 24.96% and 15.84%, respectively. In this work, it was possible to extract 93.1% of the sugars present in the olive grove pruning, with pretreatments carried out for 102 min at 156 °C with a sulfuric acid load of 4.09% (w w-1), followed by enzymatic hydrolysis performed for 174 h, with an enzyme loading of 18 PFU, 36 p-NPGU and 36 IU per gram of substrate. |
|---|---|
| Autores principais: | Pedro, Nuno |
| Outros Autores: | Bezerra, R.M.; Fraga, I.; Duarte, A.P. |
| Assunto: | Acid hydrolysis Enzymatic hydrolysis Olive pruning Pretreatment RSM Sugars |
| Ano: | 2024 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Castelo Branco |
| Idioma: | inglês |
| Origem: | Repositório Científico do Instituto Politécnico de Castelo Branco |
| _version_ | 1868350548436058112 |
|---|---|
| author | Pedro, Nuno |
| author2 | Bezerra, R.M. Fraga, I. Duarte, A.P. |
| author2_role | author author author |
| author_facet | Pedro, Nuno Bezerra, R.M. Fraga, I. Duarte, A.P. |
| author_role | author |
| contributor_name_str_mv | Repositório Científico do Instituto Politécnico de Castelo Branco |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Pedro, Nuno\",\"Person.identifier.orcid\":\"0000-0002-5403-5685\"},{\"Person.name\":\"Bezerra, R.M.\"},{\"Person.name\":\"Fraga, I.\"},{\"Person.name\":\"Duarte, A.P.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico do Instituto Politécnico de Castelo Branco |
| datacite.creators.creator.creatorName.fl_str_mv | Pedro, Nuno Bezerra, R.M. Fraga, I. Duarte, A.P. |
| datacite.date.Accepted.fl_str_mv | 2024-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2025-01-03T12:39:20Z |
| datacite.date.embargoed.fl_str_mv | 2025-01-03T12:39:20Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Acid hydrolysis Enzymatic hydrolysis Olive pruning Pretreatment RSM Sugars |
| datacite.titles.title.fl_str_mv | Production of simple sugars from olive grove pruning using acid pretreatment and enzymatic hydrolysis |
| dc.contributor.none.fl_str_mv | Repositório Científico do Instituto Politécnico de Castelo Branco |
| dc.creator.none.fl_str_mv | Pedro, Nuno Bezerra, R.M. Fraga, I. Duarte, A.P. |
| dc.date.Accepted.fl_str_mv | 2024-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2025-01-03T12:39:20Z |
| dc.date.embargoed.fl_str_mv | 2025-01-03T12:39:20Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.11/9258 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Agronomy Reserach |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Acid hydrolysis Enzymatic hydrolysis Olive pruning Pretreatment RSM Sugars |
| dc.title.fl_str_mv | Production of simple sugars from olive grove pruning using acid pretreatment and enzymatic hydrolysis |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The purpose of this paper was to optimize the production of simple sugars from olive grove pruning (OGP) using acid pretreatment and enzymatic hydrolysis. This study was based on a model composition corresponding to a 34 orthogonal factorial design and employed the response surface methodology (RSM) to optimize the pretreatment and hydrolysis conditions, aiming to attain maximum glucose, xylose and arabinose extraction from cellulose and hemicellulose of biomass. The pretreatment operating conditions considered for optimization, were temperature (60–180 °C), residence time (30–120 min) and sulphuric acid concentration (0.5–5% w w-1). Enzymatic hydrolysis experiments on solid fraction pretreated with diluted acid were performed at a solid concentration of 5% (w v-1, based on dry weight), using 50 mM citrate buffer pH 4.8 with BSA at a concentration of 60 mg g-1 dry biomass. The reaction mixture was incubated at 50 °C for 174 h on an orbital shaker at 150 rpm. Three commercial enzyme preparations (cellulase complex, b-glucosidase and xylanase) were used in enzymatic saccharification. Total carbohydrate content of the initial biomass was 51.25% (in dry mass), of which glucose was the major constituent with 33.59%. Contents of lignin and extractable found in biomass were 24.96% and 15.84%, respectively. In this work, it was possible to extract 93.1% of the sugars present in the olive grove pruning, with pretreatments carried out for 102 min at 156 °C with a sulfuric acid load of 4.09% (w w-1), followed by enzymatic hydrolysis performed for 174 h, with an enzyme loading of 18 PFU, 36 p-NPGU and 36 IU per gram of substrate. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ipcb.pt/bitstreams/0383b4cf-6398-4697-b801-c79e416fc8c3/download |
| id | ripcb_767d9cfec9526b0a8819848e55ae562c |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.11/9258 |
| instacron_str | ipcb |
| institution | Instituto Politécnico de Castelo Branco |
| instname_str | Instituto Politécnico de Castelo Branco |
| language | eng |
| network_acronym_str | ripcb |
| network_name_str | Repositório Científico do Instituto Politécnico de Castelo Branco |
| oai_identifier_str | oai:repositorio.ipcb.pt:10400.11/9258 |
| organization_str_mv | urn:organizationAcronym:ipcb |
| person_str_mv | Pedro, Nuno Pedro, Nuno https://www.ciencia-id.pt/E313-24F3-C42E E313-24F3-C42E http://orcid.org/0000-0002-5403-5685 0000-0002-5403-5685 Bezerra, R.M. Fraga, I. Duarte, A.P. |
| publishDate | 2024 |
| publisher.none.fl_str_mv | Agronomy Reserach |
| reponame_str | Repositório Científico do Instituto Politécnico de Castelo Branco |
| repository_id_str | urn:repositoryAcronym:ripcb |
| service_str_mv | urn:repositoryAcronym:ripcb |
| spelling | engAgronomy Reserachpt_PTThe purpose of this paper was to optimize the production of simple sugars from olive grove pruning (OGP) using acid pretreatment and enzymatic hydrolysis. This study was based on a model composition corresponding to a 34 orthogonal factorial design and employed the response surface methodology (RSM) to optimize the pretreatment and hydrolysis conditions, aiming to attain maximum glucose, xylose and arabinose extraction from cellulose and hemicellulose of biomass. The pretreatment operating conditions considered for optimization, were temperature (60–180 °C), residence time (30–120 min) and sulphuric acid concentration (0.5–5% w w-1). Enzymatic hydrolysis experiments on solid fraction pretreated with diluted acid were performed at a solid concentration of 5% (w v-1, based on dry weight), using 50 mM citrate buffer pH 4.8 with BSA at a concentration of 60 mg g-1 dry biomass. The reaction mixture was incubated at 50 °C for 174 h on an orbital shaker at 150 rpm. Three commercial enzyme preparations (cellulase complex, b-glucosidase and xylanase) were used in enzymatic saccharification. Total carbohydrate content of the initial biomass was 51.25% (in dry mass), of which glucose was the major constituent with 33.59%. Contents of lignin and extractable found in biomass were 24.96% and 15.84%, respectively. In this work, it was possible to extract 93.1% of the sugars present in the olive grove pruning, with pretreatments carried out for 102 min at 156 °C with a sulfuric acid load of 4.09% (w w-1), followed by enzymatic hydrolysis performed for 174 h, with an enzyme loading of 18 PFU, 36 p-NPGU and 36 IU per gram of substrate.application/pdfpt_PTProduction of simple sugars from olive grove pruning using acid pretreatment and enzymatic hydrolysisPersonalPedro, NunoDSpacehttp://dspace.org/items/93ed78e6-8872-474e-b0c7-dbc2dd6a4006DSpacehttp://dspace.org/items/93ed78e6-8872-474e-b0c7-dbc2dd6a4006PedroNunoCiência IDhttps://www.ciencia-id.ptE313-24F3-C42EORCIDhttp://orcid.org0000-0002-5403-5685Bezerra, R.M.Fraga, I.Duarte, A.P.HostingInstitutionOrganizationalRepositório Científico do Instituto Politécnico de Castelo Brancoe-mailmailto:repositorio@ipcb.ptrepositorio@ipcb.ptDOIIsPartOf10.15159/AR.24.0692025-01-03T12:39:20Z20242024-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.11/9258http://purl.org/coar/access_right/c_abf2open accessAcid hydrolysisEnzymatic hydrolysisOlive pruningPretreatmentRSMSugars530677 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ipcb.pt/bitstreams/0383b4cf-6398-4697-b801-c79e416fc8c3/downloadAgronomy Research222876886 |
| spellingShingle | Production of simple sugars from olive grove pruning using acid pretreatment and enzymatic hydrolysis Pedro, Nuno Acid hydrolysis Enzymatic hydrolysis Olive pruning Pretreatment RSM Sugars |
| status | SINGLETON |
| subject.fl_str_mv | Acid hydrolysis Enzymatic hydrolysis Olive pruning Pretreatment RSM Sugars |
| title | Production of simple sugars from olive grove pruning using acid pretreatment and enzymatic hydrolysis |
| title_full | Production of simple sugars from olive grove pruning using acid pretreatment and enzymatic hydrolysis |
| title_fullStr | Production of simple sugars from olive grove pruning using acid pretreatment and enzymatic hydrolysis |
| title_full_unstemmed | Production of simple sugars from olive grove pruning using acid pretreatment and enzymatic hydrolysis |
| title_short | Production of simple sugars from olive grove pruning using acid pretreatment and enzymatic hydrolysis |
| title_sort | Production of simple sugars from olive grove pruning using acid pretreatment and enzymatic hydrolysis |
| topic | Acid hydrolysis Enzymatic hydrolysis Olive pruning Pretreatment RSM Sugars |
| topic_facet | Acid hydrolysis Enzymatic hydrolysis Olive pruning Pretreatment RSM Sugars |
| url | http://hdl.handle.net/10400.11/9258 |
| visible | 1 |