Publicação
Survey of Mycobacterium avium subspecies paratuberculosis in road-killed wild carnivores in Portugal
| Resumo: | A survey to determine the occurrence of Mycobacterium avium subsp. paratuberculosis (MAP) in wild carnivores in Portugal was conducted by testing samples from road-killed animals between 2009 and 2012. Postmortem examinations were performed and tissues were collected from wild carnivores representing four families and six different species, with a total of 74 animals analyzed. Cultures were performed by using Löwenstein-Jensen and Middlebrook 7H11 solid media and acid-fast isolates were identified by polymerase chain reaction (PCR) and mycobactin dependency characteristics. Tissues were also screened for MAP by directly extracting DNA and testing for the MAP-specific sequences. The occurrence of infected animals (an animal had at least one tissue that was positive for culture or direct PCR) was 27.0% (n = 20). MAP was isolated from culture of 25 tissue samples (3.8%) and was detected by direct PCR in 40 (6.0%) samples. Infection was recorded in 5/6 studied species: 7/49 (14.3%) red foxes (Vulpes vulpes), 3/3 (100%) beech martens (Martes foina), 2/4 (50.0%) Eurasian otters (Lutra lutra), 7/15 (46.7%) Egyptian mongooses (Herpestes ichneumon), and 1/1 (100%) European badger (Meles meles). These species represent three different taxonomic families: Canidae (14.3% were positive), Mustelidae (75.0% were positive), and Herpestidae (46.7% were positive). The results of this study confirm the presence of MAP infection in wild carnivores in Portugal. |
|---|---|
| Autores principais: | Matos, Ana Cristina |
| Outros Autores: | Figueira, Luís; Martins, Maria Helena; Loureiro, Filipa; Pinto, Maria de Lurdes; Matos, Manuela; Coelho, Ana Cláudia |
| Assunto: | Culture Epidemiology Mycobacterium avium subsp. paratuberculosis PCR Wild carnivores |
| Ano: | 2014 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Instituto Politécnico de Castelo Branco |
| Idioma: | inglês |
| Origem: | Repositório Científico do Instituto Politécnico de Castelo Branco |
| _version_ | 1868350549566423040 |
|---|---|
| author | Matos, Ana Cristina |
| author2 | Figueira, Luís Martins, Maria Helena Loureiro, Filipa Pinto, Maria de Lurdes Matos, Manuela Coelho, Ana Cláudia |
| author2_role | author author author author author author |
| author_facet | Matos, Ana Cristina Figueira, Luís Martins, Maria Helena Loureiro, Filipa Pinto, Maria de Lurdes Matos, Manuela Coelho, Ana Cláudia |
| author_role | author |
| contributor_name_str_mv | Repositório Científico do Instituto Politécnico de Castelo Branco |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Matos, Ana Cristina\",\"Person.identifier.orcid\":\"0000-0001-9709-862X\"},{\"Person.name\":\"Figueira, Luís\",\"Person.identifier.orcid\":\"0000-0002-8454-2880\"},{\"Person.name\":\"Martins, Maria Helena\"},{\"Person.name\":\"Loureiro, Filipa\",\"Person.identifier.orcid\":\"0000-0002-5370-5333\"},{\"Person.name\":\"Pinto, Maria de Lurdes\",\"Person.identifier.orcid\":\"0000-0002-5928-6483\"},{\"Person.name\":\"Matos, Manuela\",\"Person.identifier.orcid\":\"0000-0002-9584-6636\"},{\"Person.name\":\"Coelho, Ana Cláudia\",\"Person.identifier.orcid\":\"0000-0002-7196-4179\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico do Instituto Politécnico de Castelo Branco |
| datacite.creators.creator.creatorName.fl_str_mv | Matos, Ana Cristina Figueira, Luís Martins, Maria Helena Loureiro, Filipa Pinto, Maria de Lurdes Matos, Manuela Coelho, Ana Cláudia |
| datacite.date.Accepted.fl_str_mv | 2014-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2016-03-04T12:30:15Z |
| datacite.date.embargoed.fl_str_mv | 2016-03-04T12:30:15Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Culture Epidemiology Mycobacterium avium subsp. paratuberculosis PCR Wild carnivores |
| datacite.titles.title.fl_str_mv | Survey of Mycobacterium avium subspecies paratuberculosis in road-killed wild carnivores in Portugal |
| dc.contributor.none.fl_str_mv | Repositório Científico do Instituto Politécnico de Castelo Branco |
| dc.creator.none.fl_str_mv | Matos, Ana Cristina Figueira, Luís Martins, Maria Helena Loureiro, Filipa Pinto, Maria de Lurdes Matos, Manuela Coelho, Ana Cláudia |
| dc.date.Accepted.fl_str_mv | 2014-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2016-03-04T12:30:15Z |
| dc.date.embargoed.fl_str_mv | 2016-03-04T12:30:15Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.11/3331 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Culture Epidemiology Mycobacterium avium subsp. paratuberculosis PCR Wild carnivores |
| dc.title.fl_str_mv | Survey of Mycobacterium avium subspecies paratuberculosis in road-killed wild carnivores in Portugal |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | A survey to determine the occurrence of Mycobacterium avium subsp. paratuberculosis (MAP) in wild carnivores in Portugal was conducted by testing samples from road-killed animals between 2009 and 2012. Postmortem examinations were performed and tissues were collected from wild carnivores representing four families and six different species, with a total of 74 animals analyzed. Cultures were performed by using Löwenstein-Jensen and Middlebrook 7H11 solid media and acid-fast isolates were identified by polymerase chain reaction (PCR) and mycobactin dependency characteristics. Tissues were also screened for MAP by directly extracting DNA and testing for the MAP-specific sequences. The occurrence of infected animals (an animal had at least one tissue that was positive for culture or direct PCR) was 27.0% (n = 20). MAP was isolated from culture of 25 tissue samples (3.8%) and was detected by direct PCR in 40 (6.0%) samples. Infection was recorded in 5/6 studied species: 7/49 (14.3%) red foxes (Vulpes vulpes), 3/3 (100%) beech martens (Martes foina), 2/4 (50.0%) Eurasian otters (Lutra lutra), 7/15 (46.7%) Egyptian mongooses (Herpestes ichneumon), and 1/1 (100%) European badger (Meles meles). These species represent three different taxonomic families: Canidae (14.3% were positive), Mustelidae (75.0% were positive), and Herpestidae (46.7% were positive). The results of this study confirm the presence of MAP infection in wild carnivores in Portugal. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ipcb.pt/bitstreams/edeef7d6-ba78-4932-bd3b-e969423b1152/download |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10400.11/3331 |
| instacron_str | ipcb |
| institution | Instituto Politécnico de Castelo Branco |
| instname_str | Instituto Politécnico de Castelo Branco |
| language | eng |
| network_acronym_str | ripcb |
| network_name_str | Repositório Científico do Instituto Politécnico de Castelo Branco |
| oai_identifier_str | oai:repositorio.ipcb.pt:10400.11/3331 |
| organization_str_mv | urn:organizationAcronym:ipcb |
| person_str_mv | Matos, Ana Cristina Matos, Ana Cristina https://www.ciencia-id.pt/2711-E07A-FE45 2711-E07A-FE45 http://orcid.org/0000-0001-9709-862X 0000-0001-9709-862X Figueira, Luís Figueira, Luís https://www.ciencia-id.pt/941B-C381-8158 941B-C381-8158 http://orcid.org/0000-0002-8454-2880 0000-0002-8454-2880 Martins, Maria Helena Loureiro, Filipa Loureiro, Filipa https://www.ciencia-id.pt/5B13-C797-7D38 5B13-C797-7D38 http://orcid.org/0000-0002-5370-5333 0000-0002-5370-5333 Pinto, Maria de Lurdes Pinto, Maria de Lurdes https://www.ciencia-id.pt/E81F-5A9E-5C8D E81F-5A9E-5C8D http://orcid.org/0000-0002-5928-6483 0000-0002-5928-6483 Matos, Manuela Matos, Manuela https://www.ciencia-id.pt/4F1E-ACFF-86CB 4F1E-ACFF-86CB http://orcid.org/0000-0002-9584-6636 0000-0002-9584-6636 Coelho, Ana Cláudia Coelho, Ana Cláudia https://www.ciencia-id.pt/1E10-BF40-29C5 1E10-BF40-29C5 http://orcid.org/0000-0002-7196-4179 0000-0002-7196-4179 |
| publishDate | 2014 |
| reponame_str | Repositório Científico do Instituto Politécnico de Castelo Branco |
| repository_id_str | urn:repositoryAcronym:ripcb |
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| spelling | engpt_PTA survey to determine the occurrence of Mycobacterium avium subsp. paratuberculosis (MAP) in wild carnivores in Portugal was conducted by testing samples from road-killed animals between 2009 and 2012. Postmortem examinations were performed and tissues were collected from wild carnivores representing four families and six different species, with a total of 74 animals analyzed. Cultures were performed by using Löwenstein-Jensen and Middlebrook 7H11 solid media and acid-fast isolates were identified by polymerase chain reaction (PCR) and mycobactin dependency characteristics. Tissues were also screened for MAP by directly extracting DNA and testing for the MAP-specific sequences. The occurrence of infected animals (an animal had at least one tissue that was positive for culture or direct PCR) was 27.0% (n = 20). MAP was isolated from culture of 25 tissue samples (3.8%) and was detected by direct PCR in 40 (6.0%) samples. Infection was recorded in 5/6 studied species: 7/49 (14.3%) red foxes (Vulpes vulpes), 3/3 (100%) beech martens (Martes foina), 2/4 (50.0%) Eurasian otters (Lutra lutra), 7/15 (46.7%) Egyptian mongooses (Herpestes ichneumon), and 1/1 (100%) European badger (Meles meles). These species represent three different taxonomic families: Canidae (14.3% were positive), Mustelidae (75.0% were positive), and Herpestidae (46.7% were positive). The results of this study confirm the presence of MAP infection in wild carnivores in Portugal.application/pdfpt_PTSurvey of Mycobacterium avium subspecies paratuberculosis in road-killed wild carnivores in PortugalPersonalMatos, Ana CristinaDSpacehttp://dspace.org/items/b6aa356b-8fa0-42be-af99-b34818a15cdcDSpacehttp://dspace.org/items/b6aa356b-8fa0-42be-af99-b34818a15cdcMatosAnaCiência IDhttps://www.ciencia-id.pt2711-E07A-FE45ORCIDhttp://orcid.org0000-0001-9709-862XResearcher IDhttps://www.researcherid.comK-6664-2013Scopus Author IDhttps://www.scopus.com55626078200PersonalFigueira, LuísDSpacehttp://dspace.org/items/74ef6ec4-da08-461e-a861-a4f5beb24858DSpacehttp://dspace.org/items/74ef6ec4-da08-461e-a861-a4f5beb24858FigueiraLuis Manuel FariaCiência IDhttps://www.ciencia-id.pt941B-C381-8158ORCIDhttp://orcid.org0000-0002-8454-2880Martins, Maria HelenaPersonalLoureiro, FilipaDSpacehttp://dspace.org/items/12987b24-e60e-48d2-88e7-cb013377ac19DSpacehttp://dspace.org/items/12987b24-e60e-48d2-88e7-cb013377ac19Fernandes LoureiroFilipa RaquelCiência IDhttps://www.ciencia-id.pt5B13-C797-7D38ORCIDhttp://orcid.org0000-0002-5370-5333PersonalPinto, Maria de LurdesDSpacehttp://dspace.org/items/e1a553fd-7452-47c4-a87d-f5863dd02746DSpacehttp://dspace.org/items/e1a553fd-7452-47c4-a87d-f5863dd02746Ribeiro PintoMaria de LurdesCiência IDhttps://www.ciencia-id.ptE81F-5A9E-5C8DORCIDhttp://orcid.org0000-0002-5928-6483PersonalMatos, ManuelaDSpacehttp://dspace.org/items/57055f99-449e-4fd2-9449-87d9247c4460DSpacehttp://dspace.org/items/57055f99-449e-4fd2-9449-87d9247c4460de MatosMaria Manuela OuteiroCiência IDhttps://www.ciencia-id.pt4F1E-ACFF-86CBORCIDhttp://orcid.org0000-0002-9584-6636PersonalCoelho, Ana CláudiaDSpacehttp://dspace.org/items/f0556446-0f05-4a10-ac0c-44b0c13d921cDSpacehttp://dspace.org/items/f0556446-0f05-4a10-ac0c-44b0c13d921cCoelhoAnaCiência IDhttps://www.ciencia-id.pt1E10-BF40-29C5ORCIDhttp://orcid.org0000-0002-7196-4179Researcher IDhttps://www.researcherid.comK-4740-2013Scopus Author IDhttps://www.scopus.com34970835100HostingInstitutionOrganizationalRepositório Científico do Instituto Politécnico de Castelo Brancoe-mailmailto:repositorio@ipcb.ptrepositorio@ipcb.ptISSNIsPartOf1937-2825DOIIsPartOf10.1638/2014-0010.12016-03-04T12:30:15Z20142014-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.11/3331http://purl.org/coar/access_right/c_16ecrestricted accessCultureEpidemiologyMycobacterium avium subsp. paratuberculosisPCRWild carnivores66767 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ipcb.pt/bitstreams/edeef7d6-ba78-4932-bd3b-e969423b1152/downloadJournal of Zoo and Wildlife Medicine45775781USA |
| spellingShingle | Survey of Mycobacterium avium subspecies paratuberculosis in road-killed wild carnivores in Portugal Matos, Ana Cristina Culture Epidemiology Mycobacterium avium subsp. paratuberculosis PCR Wild carnivores |
| status | SINGLETON |
| subject.fl_str_mv | Culture Epidemiology Mycobacterium avium subsp. paratuberculosis PCR Wild carnivores |
| title | Survey of Mycobacterium avium subspecies paratuberculosis in road-killed wild carnivores in Portugal |
| title_full | Survey of Mycobacterium avium subspecies paratuberculosis in road-killed wild carnivores in Portugal |
| title_fullStr | Survey of Mycobacterium avium subspecies paratuberculosis in road-killed wild carnivores in Portugal |
| title_full_unstemmed | Survey of Mycobacterium avium subspecies paratuberculosis in road-killed wild carnivores in Portugal |
| title_short | Survey of Mycobacterium avium subspecies paratuberculosis in road-killed wild carnivores in Portugal |
| title_sort | Survey of Mycobacterium avium subspecies paratuberculosis in road-killed wild carnivores in Portugal |
| topic | Culture Epidemiology Mycobacterium avium subsp. paratuberculosis PCR Wild carnivores |
| topic_facet | Culture Epidemiology Mycobacterium avium subsp. paratuberculosis PCR Wild carnivores |
| url | http://hdl.handle.net/10400.11/3331 |
| visible | 1 |