Publicação

Characterization of a polyubiquitin gene in T. thermophila and of ubiquitin gene expression during sexual reproduction and under stress conditions

Ver documento

Detalhes bibliográficos
Resumo:A 5-unit polyubiquitin gene, TTU3, was isolated from a T. thermophila genomic library and sequenced. This gene presents an extra triplet coding for Phe, a AGAGA motif and a putative HSE element in its 5'-non-coding region. The ubiquitin gene expression in this ciliate was investigated by Northern blot hybridization in conjugating cells or cells under stress conditions. Exponentially growing cells express two ubiquitin mRNAs of 0.75 and 1.8 kb and a new species of 1.4 kb is induced under hyperthermic stress. During sexual reproduction of the cells (conjugation) the 1.8-kb mRNA is still transcribed whereas the steady-state population of the 0.75 mRNA transcripts is strongly diminished. Southern blot analysis suggests that ubiquitin in T. thermophila constitutes a large family of about ten members.
Autores principais:Guerreiro, Paulo
Outros Autores:Rodrigues-Pousada, Claudina
Assunto:Ubiquitin mRNAs Genomic organization Tetrahymena thermophila Ciliate Hyperthermic stress Amino acid sequence Base sequence Cloning, Molecular Gene dosage Gene expression regulation Molecular sequence data RNA, Messenger Sequence analysis
Ano:1996
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Instituto Politécnico de Lisboa
Idioma:inglês
Origem:Repositório Científico do Instituto Politécnico de Lisboa

Registos relacionados

Registos relacionados