Publicação
The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1
| Resumo: | Centrosomes are organelles consisting of two structurally and functionally distinct centrioles, with the mother centriole having complex distal (DA) and subdistal appendages (SDA). Despite their importance, how appendages are assembled and maintained remains unclear. This study investigated human TBCCD1, a centrosomal protein essential for centrosome positioning, to uncover its localization and role at centrioles. We found that TBCCD1 localizes at both proximal and distal regions of the two centrioles, forming a complex structure spanning from SDA to DA and extending inside and outside the centriole lumen. TBCCD1 depletion caused centrosome mispositioning, which was partially rescued by taxol, and the loss of microtubules (MTs) anchored to centrosomes. TBCCD1 depletion also reduced levels of SDA proteins involved in MT anchoring such as Centriolin/CEP110, Ninein, and CEP170. Additionally, TBCCD1 was essential for the correct positioning of motile cilia basal bodies and associated structures in Paramecium. This study reveals that TBCCD1 is an evolutionarily conserved protein essential for centriole and basal body localization and appendage assembly and maintenance. A BioID screening also linked TBCCD1 to ciliopathy-associated protein networks. |
|---|---|
| Autores principais: | Carmona, Bruno |
| Outros Autores: | Camelo, Carolina; Mehraz, Manon; Lemullois, Michel; Faria, Mariana Lince; Coyaud, Étienne; Marinho, H. Susana; Gonçalves, João; Nolasco, Sofia; Pinto, Francisco; Raught, Brian; Tassin, Anne-Marie; Koll, France; Soares, Helena |
| Assunto: | TBCCD1 Centrosome Centriole appendages Cilia Basal body Microtubules Paramecium |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | preprint |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Científico do Instituto Politécnico de Lisboa |
| _version_ | 1866887643717959680 |
|---|---|
| author | Carmona, Bruno |
| author2 | Camelo, Carolina Mehraz, Manon Lemullois, Michel Faria, Mariana Lince Coyaud, Étienne Marinho, H. Susana Gonçalves, João Nolasco, Sofia Pinto, Francisco Raught, Brian Tassin, Anne-Marie Koll, France Soares, Helena |
| author2_role | author author author author author author author author author author author author author |
| author_facet | Carmona, Bruno Camelo, Carolina Mehraz, Manon Lemullois, Michel Faria, Mariana Lince Coyaud, Étienne Marinho, H. Susana Gonçalves, João Nolasco, Sofia Pinto, Francisco Raught, Brian Tassin, Anne-Marie Koll, France Soares, Helena |
| author_role | author |
| contributor_name_str_mv | RCIPL |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Carmona, Bruno\",\"Person.identifier.orcid\":\"0000-0003-0871-9063\"},{\"Person.name\":\"Camelo, Carolina\"},{\"Person.name\":\"Mehraz, Manon\"},{\"Person.name\":\"Lemullois, Michel\"},{\"Person.name\":\"Faria, Mariana Lince\"},{\"Person.name\":\"Coyaud, Étienne\"},{\"Person.name\":\"Marinho, H. Susana\"},{\"Person.name\":\"Gonçalves, João\"},{\"Person.name\":\"Nolasco, Sofia\"},{\"Person.name\":\"Pinto, Francisco\"},{\"Person.name\":\"Raught, Brian\"},{\"Person.name\":\"Tassin, Anne-Marie\"},{\"Person.name\":\"Koll, France\"},{\"Person.name\":\"Soares, Helena\",\"Person.identifier.orcid\":\"0000-0001-6180-7041\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RCIPL |
| datacite.creators.creator.creatorName.fl_str_mv | Carmona, Bruno Camelo, Carolina Mehraz, Manon Lemullois, Michel Faria, Mariana Lince Coyaud, Étienne Marinho, H. Susana Gonçalves, João Nolasco, Sofia Pinto, Francisco Raught, Brian Tassin, Anne-Marie Koll, France Soares, Helena |
| datacite.date.Accepted.fl_str_mv | 2023-07-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-08-22T13:52:43Z |
| datacite.date.embargoed.fl_str_mv | 2023-08-22T13:52:43Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | TBCCD1 Centrosome Centriole appendages Cilia Basal body Microtubules Paramecium |
| datacite.titles.title.fl_str_mv | The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1 |
| dc.contributor.none.fl_str_mv | RCIPL |
| dc.creator.none.fl_str_mv | Carmona, Bruno Camelo, Carolina Mehraz, Manon Lemullois, Michel Faria, Mariana Lince Coyaud, Étienne Marinho, H. Susana Gonçalves, João Nolasco, Sofia Pinto, Francisco Raught, Brian Tassin, Anne-Marie Koll, France Soares, Helena |
| dc.date.Accepted.fl_str_mv | 2023-07-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-08-22T13:52:43Z |
| dc.date.embargoed.fl_str_mv | 2023-08-22T13:52:43Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.21/16386 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | TBCCD1 Centrosome Centriole appendages Cilia Basal body Microtubules Paramecium |
| dc.title.fl_str_mv | The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1 |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_816b |
| description | Centrosomes are organelles consisting of two structurally and functionally distinct centrioles, with the mother centriole having complex distal (DA) and subdistal appendages (SDA). Despite their importance, how appendages are assembled and maintained remains unclear. This study investigated human TBCCD1, a centrosomal protein essential for centrosome positioning, to uncover its localization and role at centrioles. We found that TBCCD1 localizes at both proximal and distal regions of the two centrioles, forming a complex structure spanning from SDA to DA and extending inside and outside the centriole lumen. TBCCD1 depletion caused centrosome mispositioning, which was partially rescued by taxol, and the loss of microtubules (MTs) anchored to centrosomes. TBCCD1 depletion also reduced levels of SDA proteins involved in MT anchoring such as Centriolin/CEP110, Ninein, and CEP170. Additionally, TBCCD1 was essential for the correct positioning of motile cilia basal bodies and associated structures in Paramecium. This study reveals that TBCCD1 is an evolutionarily conserved protein essential for centriole and basal body localization and appendage assembly and maintenance. A BioID screening also linked TBCCD1 to ciliopathy-associated protein networks. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | preprint |
| fulltext.url.fl_str_mv | https://repositorio.ipl.pt/bitstreams/02e9c584-700d-4dfd-b1a8-abcdfe72ef6d/download |
| id | ripl_6acfa11a2c7a8bd4ecebdaeeba555321 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.21/16386 |
| instacron_str | ipl |
| institution | Instituto Politécnico de Lisboa |
| instname_str | Instituto Politécnico de Lisboa |
| language | eng |
| network_acronym_str | ripl |
| network_name_str | Repositório Científico do Instituto Politécnico de Lisboa |
| oai_identifier_str | oai:repositorio.ipl.pt:10400.21/16386 |
| organization_str_mv | urn:organizationAcronym:ipl |
| person_str_mv | Carmona, Bruno Carmona, Bruno https://www.ciencia-id.pt/681F-6045-F8C2 681F-6045-F8C2 http://orcid.org/0000-0003-0871-9063 0000-0003-0871-9063 Camelo, Carolina Mehraz, Manon Lemullois, Michel Faria, Mariana Lince Coyaud, Étienne Marinho, H. Susana Gonçalves, João Nolasco, Sofia Pinto, Francisco Raught, Brian Tassin, Anne-Marie Koll, France Soares, Helena Soares, Helena https://www.ciencia-id.pt/131B-F0E1-572C 131B-F0E1-572C http://orcid.org/0000-0001-6180-7041 0000-0001-6180-7041 |
| publishDate | 2023 |
| reponame_str | Repositório Científico do Instituto Politécnico de Lisboa |
| repository_id_str | urn:repositoryAcronym:ripl |
| service_str_mv | urn:repositoryAcronym:ripl |
| spelling | engpt_PTCentrosomes are organelles consisting of two structurally and functionally distinct centrioles, with the mother centriole having complex distal (DA) and subdistal appendages (SDA). Despite their importance, how appendages are assembled and maintained remains unclear. This study investigated human TBCCD1, a centrosomal protein essential for centrosome positioning, to uncover its localization and role at centrioles. We found that TBCCD1 localizes at both proximal and distal regions of the two centrioles, forming a complex structure spanning from SDA to DA and extending inside and outside the centriole lumen. TBCCD1 depletion caused centrosome mispositioning, which was partially rescued by taxol, and the loss of microtubules (MTs) anchored to centrosomes. TBCCD1 depletion also reduced levels of SDA proteins involved in MT anchoring such as Centriolin/CEP110, Ninein, and CEP170. Additionally, TBCCD1 was essential for the correct positioning of motile cilia basal bodies and associated structures in Paramecium. This study reveals that TBCCD1 is an evolutionarily conserved protein essential for centriole and basal body localization and appendage assembly and maintenance. A BioID screening also linked TBCCD1 to ciliopathy-associated protein networks.application/pdfpt_PTThe maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1PersonalCarmona, BrunoDSpacehttp://dspace.org/items/908e548e-eaac-4485-97c5-fcbd33fe7e5aDSpacehttp://dspace.org/items/908e548e-eaac-4485-97c5-fcbd33fe7e5aSousa CarmonaBruno FilipeCiência IDhttps://www.ciencia-id.pt681F-6045-F8C2ORCIDhttp://orcid.org0000-0003-0871-9063Camelo, CarolinaMehraz, ManonLemullois, MichelFaria, Mariana LinceCoyaud, ÉtienneMarinho, H. SusanaGonçalves, JoãoNolasco, SofiaPinto, FranciscoRaught, BrianTassin, Anne-MarieKoll, FrancePersonalSoares, HelenaDSpacehttp://dspace.org/items/267fae06-39c1-4b12-a246-39e0b1dde34aDSpacehttp://dspace.org/items/267fae06-39c1-4b12-a246-39e0b1dde34aAntunes SoaresMaria HelenaCiência IDhttps://www.ciencia-id.pt131B-F0E1-572CORCIDhttp://orcid.org0000-0001-6180-7041Scopus Author IDhttps://www.scopus.com55932139400HostingInstitutionOrganizationalRCIPLe-mailmailto:rcaap@sp.ipl.ptrcaap@sp.ipl.ptDOIIsPartOf10.1101/2023.07.26.5496472023-08-22T13:52:43Z2023-072023-07-01T00:00:00ZHandlehttp://hdl.handle.net/10400.21/16386http://purl.org/coar/access_right/c_abf2open accessTBCCD1CentrosomeCentriole appendagesCiliaBasal bodyMicrotubulesParamecium7728078 bytesother research producthttp://purl.org/coar/resource_type/c_816bpreprint2023-07http://creativecommons.org/licenses/by-nc-nd/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ipl.pt/bitstreams/02e9c584-700d-4dfd-b1a8-abcdfe72ef6d/download |
| spellingShingle | The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1 Carmona, Bruno TBCCD1 Centrosome Centriole appendages Cilia Basal body Microtubules Paramecium |
| status | SINGLETON |
| subject.fl_str_mv | TBCCD1 Centrosome Centriole appendages Cilia Basal body Microtubules Paramecium |
| title | The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1 |
| title_full | The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1 |
| title_fullStr | The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1 |
| title_full_unstemmed | The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1 |
| title_short | The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1 |
| title_sort | The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1 |
| topic | TBCCD1 Centrosome Centriole appendages Cilia Basal body Microtubules Paramecium |
| topic_facet | TBCCD1 Centrosome Centriole appendages Cilia Basal body Microtubules Paramecium |
| url | http://hdl.handle.net/10400.21/16386 |
| visible | 1 |