Publicação
Bioburden in sleeping environments from Portuguese dwellings
| Resumo: | A wider characterization of indoor air quality during sleep is still lacking in the literature. This study intends to assess bioburden before and after sleeping periods in Portuguese dwellings through active methods (air sampling) coupled with passive methods, such as electrostatic dust cloths (EDC); and investigate associations between before and after sleeping and bioburden. In addition, and driven by the lack of information regarding fungi azole-resistance in Portuguese dwellings, a screening with supplemented media was also performed. The most prevalent genera of airborne bacteria identified in the indoor air of the bedrooms were Micrococcus (41%), Staphylococcus (15%) and Neisseria (9%). The major indoor bacterial species isolated in all ten studied bedrooms were Micrococcus luteus (30%), Staphylococcus aureus (13%) and Micrococcus varians (11%). Our results highlight that our bodies are the source of the majority of the bacteria found in the indoor air of our homes. Regarding air fungal contamination, Chrysosporium spp. presented the highest prevalence both in after the sleeping period (40.8%) and before the sleeping period (28.8%) followed by Penicillium spp. (23.47% morning; 23.6% night) and Chrysonilia spp. (12.4% morning; 20.3% night). Several Aspergillus sections were identified in air and EDC samples. However, none of the fungal species/strains (Aspergillus sections Fumigati, Flavi, Nidulantes and Circumdati) were amplified by qPCR in the analyzed EDC. The correlations observed suggest reduced susceptibility to antifungal drugs of some fungal species found in sleeping environments. Toxigenic fungal species and indicators of harmful fungal contamination were observed in sleeping environments. |
|---|---|
| Autores principais: | Viegas, Carla |
| Outros Autores: | Dias, Marta; Monteiro, Ana; Faria, Tiago; Lage, Joana; Carolino, Elisabete; Caetano, Liliana Aranha; Gomes, Anita Q.; Almeida, Susana Marta; Verde, Sandra Cabo; Belo, Joana; Canha, Nuno |
| Assunto: | Indoor air Air quality Sleep environment Fungi Bacteria Aspergillus Fungi azole-resistance screening IPL/2017/E2SLEEP/ESTeSL/711030 IPL/2020/BIO-AmbuDrivers_ESTeSL UIDB/05608/2020 UIDP/05608/2020 |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Instituto Politécnico de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Científico do Instituto Politécnico de Lisboa |
| _version_ | 1866887616367951872 |
|---|---|
| author | Viegas, Carla |
| author2 | Dias, Marta Monteiro, Ana Faria, Tiago Lage, Joana Carolino, Elisabete Caetano, Liliana Aranha Gomes, Anita Q. Almeida, Susana Marta Verde, Sandra Cabo Belo, Joana Canha, Nuno |
| author2_role | author author author author author author author author author author author |
| author_facet | Viegas, Carla Dias, Marta Monteiro, Ana Faria, Tiago Lage, Joana Carolino, Elisabete Caetano, Liliana Aranha Gomes, Anita Q. Almeida, Susana Marta Verde, Sandra Cabo Belo, Joana Canha, Nuno |
| author_role | author |
| contributor_name_str_mv | RCIPL |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Viegas, Carla\",\"Person.identifier.orcid\":\"0000-0002-1545-6479\"},{\"Person.name\":\"Dias, Marta\"},{\"Person.name\":\"Monteiro, Ana\"},{\"Person.name\":\"Faria, Tiago\"},{\"Person.name\":\"Lage, Joana\"},{\"Person.name\":\"Carolino, Elisabete\",\"Person.identifier.orcid\":\"0000-0003-4165-7052\"},{\"Person.name\":\"Caetano, Liliana Aranha\",\"Person.identifier.orcid\":\"0000-0003-1496-2609\"},{\"Person.name\":\"Gomes, Anita Q.\",\"Person.identifier.orcid\":\"0000-0002-3348-0448\"},{\"Person.name\":\"Almeida, Susana Marta\"},{\"Person.name\":\"Verde, Sandra Cabo\"},{\"Person.name\":\"Belo, Joana\",\"Person.identifier.orcid\":\"0000-0003-2766-706X\"},{\"Person.name\":\"Canha, Nuno\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RCIPL |
| datacite.creators.creator.creatorName.fl_str_mv | Viegas, Carla Dias, Marta Monteiro, Ana Faria, Tiago Lage, Joana Carolino, Elisabete Caetano, Liliana Aranha Gomes, Anita Q. Almeida, Susana Marta Verde, Sandra Cabo Belo, Joana Canha, Nuno |
| datacite.date.Accepted.fl_str_mv | 2021-03-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2021-02-11T17:08:12Z |
| datacite.date.embargoed.fl_str_mv | 2021-02-11T17:08:12Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Indoor air Air quality Sleep environment Fungi Bacteria Aspergillus Fungi azole-resistance screening IPL/2017/E2SLEEP/ESTeSL/711030 IPL/2020/BIO-AmbuDrivers_ESTeSL UIDB/05608/2020 UIDP/05608/2020 |
| datacite.titles.title.fl_str_mv | Bioburden in sleeping environments from Portuguese dwellings |
| dc.contributor.none.fl_str_mv | RCIPL |
| dc.creator.none.fl_str_mv | Viegas, Carla Dias, Marta Monteiro, Ana Faria, Tiago Lage, Joana Carolino, Elisabete Caetano, Liliana Aranha Gomes, Anita Q. Almeida, Susana Marta Verde, Sandra Cabo Belo, Joana Canha, Nuno |
| dc.date.Accepted.fl_str_mv | 2021-03-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2021-02-11T17:08:12Z |
| dc.date.embargoed.fl_str_mv | 2021-02-11T17:08:12Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.21/12824 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Indoor air Air quality Sleep environment Fungi Bacteria Aspergillus Fungi azole-resistance screening IPL/2017/E2SLEEP/ESTeSL/711030 IPL/2020/BIO-AmbuDrivers_ESTeSL UIDB/05608/2020 UIDP/05608/2020 |
| dc.title.fl_str_mv | Bioburden in sleeping environments from Portuguese dwellings |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | A wider characterization of indoor air quality during sleep is still lacking in the literature. This study intends to assess bioburden before and after sleeping periods in Portuguese dwellings through active methods (air sampling) coupled with passive methods, such as electrostatic dust cloths (EDC); and investigate associations between before and after sleeping and bioburden. In addition, and driven by the lack of information regarding fungi azole-resistance in Portuguese dwellings, a screening with supplemented media was also performed. The most prevalent genera of airborne bacteria identified in the indoor air of the bedrooms were Micrococcus (41%), Staphylococcus (15%) and Neisseria (9%). The major indoor bacterial species isolated in all ten studied bedrooms were Micrococcus luteus (30%), Staphylococcus aureus (13%) and Micrococcus varians (11%). Our results highlight that our bodies are the source of the majority of the bacteria found in the indoor air of our homes. Regarding air fungal contamination, Chrysosporium spp. presented the highest prevalence both in after the sleeping period (40.8%) and before the sleeping period (28.8%) followed by Penicillium spp. (23.47% morning; 23.6% night) and Chrysonilia spp. (12.4% morning; 20.3% night). Several Aspergillus sections were identified in air and EDC samples. However, none of the fungal species/strains (Aspergillus sections Fumigati, Flavi, Nidulantes and Circumdati) were amplified by qPCR in the analyzed EDC. The correlations observed suggest reduced susceptibility to antifungal drugs of some fungal species found in sleeping environments. Toxigenic fungal species and indicators of harmful fungal contamination were observed in sleeping environments. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ipl.pt/bitstreams/dbbfc877-f411-4be6-bb84-d8d756a7b34a/download |
| id | ripl_8decd4753bf899c193704af18880e2cc |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.21/12824 |
| instacron_str | ipl |
| institution | Instituto Politécnico de Lisboa |
| instname_str | Instituto Politécnico de Lisboa |
| language | eng |
| network_acronym_str | ripl |
| network_name_str | Repositório Científico do Instituto Politécnico de Lisboa |
| oai_identifier_str | oai:repositorio.ipl.pt:10400.21/12824 |
| organization_str_mv | urn:organizationAcronym:ipl |
| person_str_mv | Viegas, Carla Viegas, Carla https://www.ciencia-id.pt/EE1E-C639-D70F EE1E-C639-D70F http://orcid.org/0000-0002-1545-6479 0000-0002-1545-6479 Dias, Marta Monteiro, Ana Faria, Tiago Lage, Joana Carolino, Elisabete Carolino, Elisabete https://www.ciencia-id.pt/1216-EFA3-1E0F 1216-EFA3-1E0F http://orcid.org/0000-0003-4165-7052 0000-0003-4165-7052 Caetano, Liliana Aranha Caetano, Liliana Aranha https://www.ciencia-id.pt/9716-9DAC-532A 9716-9DAC-532A http://orcid.org/0000-0003-1496-2609 0000-0003-1496-2609 Gomes, Anita Q. Gomes, Anita Q. https://www.ciencia-id.pt/4B10-E015-52B7 4B10-E015-52B7 http://orcid.org/0000-0002-3348-0448 0000-0002-3348-0448 Almeida, Susana Marta Verde, Sandra Cabo Belo, Joana Belo, Joana https://www.ciencia-id.pt/021C-8ADF-15D3 021C-8ADF-15D3 http://orcid.org/0000-0003-2766-706X 0000-0003-2766-706X Canha, Nuno |
| publishDate | 2021 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | Repositório Científico do Instituto Politécnico de Lisboa |
| repository_id_str | urn:repositoryAcronym:ripl |
| service_str_mv | urn:repositoryAcronym:ripl |
| spelling | engElsevierpt_PTA wider characterization of indoor air quality during sleep is still lacking in the literature. This study intends to assess bioburden before and after sleeping periods in Portuguese dwellings through active methods (air sampling) coupled with passive methods, such as electrostatic dust cloths (EDC); and investigate associations between before and after sleeping and bioburden. In addition, and driven by the lack of information regarding fungi azole-resistance in Portuguese dwellings, a screening with supplemented media was also performed. The most prevalent genera of airborne bacteria identified in the indoor air of the bedrooms were Micrococcus (41%), Staphylococcus (15%) and Neisseria (9%). The major indoor bacterial species isolated in all ten studied bedrooms were Micrococcus luteus (30%), Staphylococcus aureus (13%) and Micrococcus varians (11%). Our results highlight that our bodies are the source of the majority of the bacteria found in the indoor air of our homes. Regarding air fungal contamination, Chrysosporium spp. presented the highest prevalence both in after the sleeping period (40.8%) and before the sleeping period (28.8%) followed by Penicillium spp. (23.47% morning; 23.6% night) and Chrysonilia spp. (12.4% morning; 20.3% night). Several Aspergillus sections were identified in air and EDC samples. However, none of the fungal species/strains (Aspergillus sections Fumigati, Flavi, Nidulantes and Circumdati) were amplified by qPCR in the analyzed EDC. The correlations observed suggest reduced susceptibility to antifungal drugs of some fungal species found in sleeping environments. Toxigenic fungal species and indicators of harmful fungal contamination were observed in sleeping environments.application/pdfpt_PTBioburden in sleeping environments from Portuguese dwellingsPersonalViegas, CarlaDSpacehttp://dspace.org/items/b5fa5da4-50c3-4b88-ae20-1bc63cb485f7DSpacehttp://dspace.org/items/b5fa5da4-50c3-4b88-ae20-1bc63cb485f7ViegasCarlaCiência IDhttps://www.ciencia-id.ptEE1E-C639-D70FORCIDhttp://orcid.org0000-0002-1545-6479Researcher IDhttps://www.researcherid.comB-7217-2013Researcher IDhttps://www.researcherid.comB-7217-2013Scopus Author IDhttps://www.scopus.com55443609700Scopus Author IDhttps://www.scopus.com35270760200Dias, MartaMonteiro, AnaFaria, TiagoLage, JoanaPersonalCarolino, ElisabeteDSpacehttp://dspace.org/items/77930d39-ed34-44dc-a4a6-9bf833e5e688DSpacehttp://dspace.org/items/77930d39-ed34-44dc-a4a6-9bf833e5e688CarolinoElisabeteCiência IDhttps://www.ciencia-id.pt1216-EFA3-1E0FORCIDhttp://orcid.org0000-0003-4165-7052Researcher IDhttps://www.researcherid.comF-1012-2015Scopus Author IDhttps://www.scopus.com25821697000PersonalCaetano, Liliana AranhaDSpacehttp://dspace.org/items/6517c656-f913-4f54-8682-77c2856c9e4cDSpacehttp://dspace.org/items/6517c656-f913-4f54-8682-77c2856c9e4cAranha CaetanoLilianaCiência IDhttps://www.ciencia-id.pt9716-9DAC-532AORCIDhttp://orcid.org0000-0003-1496-2609PersonalGomes, Anita Q.DSpacehttp://dspace.org/items/2b5a3f0a-29c2-4ecd-a83f-50d0b99a4875DSpacehttp://dspace.org/items/2b5a3f0a-29c2-4ecd-a83f-50d0b99a4875GomesAnitaCiência IDhttps://www.ciencia-id.pt4B10-E015-52B7ORCIDhttp://orcid.org0000-0002-3348-0448Researcher IDhttps://www.researcherid.comC-3580-2014Scopus Author IDhttps://www.scopus.com7202386033Almeida, Susana MartaVerde, Sandra CaboPersonalBelo, JoanaDSpacehttp://dspace.org/items/867228ff-013f-43c0-9768-23595c18f7d7DSpacehttp://dspace.org/items/867228ff-013f-43c0-9768-23595c18f7d7BeloJoanaCiência IDhttps://www.ciencia-id.pt021C-8ADF-15D3ORCIDhttp://orcid.org0000-0003-2766-706XCanha, NunoHostingInstitutionOrganizationalRCIPLe-mailmailto:rcaap@sp.ipl.ptrcaap@sp.ipl.ptDOIIsPartOf10.1016/j.envpol.2020.1164172021-02-11T17:08:12Z2021-032021-03-01T00:00:00ZHandlehttp://hdl.handle.net/10400.21/12824http://purl.org/coar/access_right/c_16ecrestricted accessIndoor airAir qualitySleep environmentFungiBacteriaAspergillusFungi azole-resistance screeningIPL/2017/E2SLEEP/ESTeSL/711030IPL/2020/BIO-AmbuDrivers_ESTeSLUIDB/05608/2020UIDP/05608/2020818417 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2021-03http://creativecommons.org/licenses/by-nc-nd/4.0/http://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ipl.pt/bitstreams/dbbfc877-f411-4be6-bb84-d8d756a7b34a/downloadEnvironmental Pollution273116417 |
| spellingShingle | Bioburden in sleeping environments from Portuguese dwellings Viegas, Carla Indoor air Air quality Sleep environment Fungi Bacteria Aspergillus Fungi azole-resistance screening IPL/2017/E2SLEEP/ESTeSL/711030 IPL/2020/BIO-AmbuDrivers_ESTeSL UIDB/05608/2020 UIDP/05608/2020 |
| status | SINGLETON |
| subject.fl_str_mv | Indoor air Air quality Sleep environment Fungi Bacteria Aspergillus Fungi azole-resistance screening IPL/2017/E2SLEEP/ESTeSL/711030 IPL/2020/BIO-AmbuDrivers_ESTeSL UIDB/05608/2020 UIDP/05608/2020 |
| title | Bioburden in sleeping environments from Portuguese dwellings |
| title_full | Bioburden in sleeping environments from Portuguese dwellings |
| title_fullStr | Bioburden in sleeping environments from Portuguese dwellings |
| title_full_unstemmed | Bioburden in sleeping environments from Portuguese dwellings |
| title_short | Bioburden in sleeping environments from Portuguese dwellings |
| title_sort | Bioburden in sleeping environments from Portuguese dwellings |
| topic | Indoor air Air quality Sleep environment Fungi Bacteria Aspergillus Fungi azole-resistance screening IPL/2017/E2SLEEP/ESTeSL/711030 IPL/2020/BIO-AmbuDrivers_ESTeSL UIDB/05608/2020 UIDP/05608/2020 |
| topic_facet | Indoor air Air quality Sleep environment Fungi Bacteria Aspergillus Fungi azole-resistance screening IPL/2017/E2SLEEP/ESTeSL/711030 IPL/2020/BIO-AmbuDrivers_ESTeSL UIDB/05608/2020 UIDP/05608/2020 |
| url | http://hdl.handle.net/10400.21/12824 |
| visible | 1 |