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Impact of epigallocatechinn-3-gallate on Staphylococcus aureus molecular profile

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Resumo:The discovery of new antimicrobial compounds is critical for the control of severe nosocomial infections, as those associated with methicillin-sensitive (MSSA) and resistant (MRSA) Staphylococcus aureus strains. In order to enhance new therapeutic approaches, it is crucial to develop new platforms to screen innovative compounds. Here, we evaluated how Fourier Transform Infra-Red (FTIR)-spectroscopy enables the prediction of antibiotic resistance and monitors the impact of epigallocatechin-3-gallate (EGCG) on the metabolism of MRSA and MSSA strains. Data showed that EGCG impacts the bacteria's metabolism, and that MRSA strains are more sensitive to EGCG. The high sensitivity of the technique also enabled us to discriminate the impact of EGCG concentrations, i.e., between 25 and 50, and between 50 and 100μg/ml. On the other hand, EGCG's impact on cellular molecular composition was lower than the differences between MSSA and MRSA strains. Furthermore, it was possible to predict these strains' resistance towards the antibiotics methicillin, amoxicillin, imipenem, and gentamicin. Since the spectra were acquired in a rapid, simple, economic, and high-throughput mode, this methodology may strongly promote the surveillance of nosocomial infection caused by S. aureus, and to screen new antimicrobial compounds.
Autores principais:Inácio, Raquel
Outros Autores:Ribeiro, Edna; Calado, Cecília R. C.
Assunto:FCT_UIDP/05608/2020 FCT_UIDB/05608/2020 Nosocomial infections Staphylococcus aureus FTIR-spectroscopy Epigallocatechin-3-gallate
Ano:2025
País:Portugal
Tipo de documento:comunicação em conferência
Tipo de acesso:acesso embargado
Instituição associada:Instituto Politécnico de Lisboa
Idioma:inglês
Origem:Repositório Científico do Instituto Politécnico de Lisboa

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