Publicação
In situ NIR spectroscopy monitoring of plasmid production processes effect of producing strain, medium composition and the cultivation strategy
| Resumo: | BACKGROUNDWhile the pharmaceutical industry keeps an eye on plasmid DNA production for new generation gene therapies, real-time monitoring techniques for plasmid bioproduction are as yet unavailable. This work shows the possibility of in situ monitoring of plasmid production in Escherichia coli cultures using a near infrared (NIR) fiber optic probe. RESULTSPartial least squares (PLS) regression models based on the NIR spectra were developed for predicting bioprocess critical variables such as the concentrations of biomass, plasmid, carbon sources (glucose and glycerol) and acetate. In order to achieve robust models able to predict the performance of plasmid production processes, independently of the composition of the cultivation medium, cultivation strategy (batch versus fed-batch) and E. coli strain used, three strategies were adopted, using: (i) E. coliDH5 cultures conducted under different media compositions and culture strategies (batch and fed-batch); (ii) engineered E. coli strains, MG1655endArecApgi and MG1655endArecA, grown on the same medium and culture strategy; (iii) diverse E. coli strains, over batch and fed-batch cultivations and using different media compositions. PLS models showed high accuracy for predicting all variables in the three groups of cultures. CONCLUSIONNIR spectroscopy combined with PLS modeling provides a fast, inexpensive and contamination-free technique to accurately monitoring plasmid bioprocesses in real time, independently of the medium composition, cultivation strategy and the E. coli strain used. |
|---|---|
| Autores principais: | Lopes, Marta B. |
| Outros Autores: | Gonçalves, Geisa A. L.; Felício-Silva, Daniel; Prather, Kristala L. J.; Monteiro, Gabriel; Prazeres, Duarte M. F.; Calado, Cecília |
| Assunto: | Plasmid bioprocess Escherichia coli Near infrared spectroscopy In situ monitoring |
| Ano: | 2015 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Instituto Politécnico de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Científico do Instituto Politécnico de Lisboa |
| _version_ | 1866887434605690880 |
|---|---|
| author | Lopes, Marta B. |
| author2 | Gonçalves, Geisa A. L. Felício-Silva, Daniel Prather, Kristala L. J. Monteiro, Gabriel Prazeres, Duarte M. F. Calado, Cecília |
| author2_role | author author author author author author |
| author_facet | Lopes, Marta B. Gonçalves, Geisa A. L. Felício-Silva, Daniel Prather, Kristala L. J. Monteiro, Gabriel Prazeres, Duarte M. F. Calado, Cecília |
| author_role | author |
| contributor_name_str_mv | RCIPL |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Lopes, Marta B.\"},{\"Person.name\":\"Gonçalves, Geisa A. L.\"},{\"Person.name\":\"Felício-Silva, Daniel\"},{\"Person.name\":\"Prather, Kristala L. J.\"},{\"Person.name\":\"Monteiro, Gabriel\"},{\"Person.name\":\"Prazeres, Duarte M. F.\"},{\"Person.name\":\"Calado, Cecília\",\"Person.identifier.orcid\":\"0000-0002-5264-9755\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RCIPL |
| datacite.creators.creator.creatorName.fl_str_mv | Lopes, Marta B. Gonçalves, Geisa A. L. Felício-Silva, Daniel Prather, Kristala L. J. Monteiro, Gabriel Prazeres, Duarte M. F. Calado, Cecília |
| datacite.date.Accepted.fl_str_mv | 2015-02-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2016-04-20T14:20:47Z |
| datacite.date.embargoed.fl_str_mv | 2016-04-20T14:20:47Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Plasmid bioprocess Escherichia coli Near infrared spectroscopy In situ monitoring |
| datacite.titles.title.fl_str_mv | In situ NIR spectroscopy monitoring of plasmid production processes effect of producing strain, medium composition and the cultivation strategy |
| dc.contributor.none.fl_str_mv | RCIPL |
| dc.creator.none.fl_str_mv | Lopes, Marta B. Gonçalves, Geisa A. L. Felício-Silva, Daniel Prather, Kristala L. J. Monteiro, Gabriel Prazeres, Duarte M. F. Calado, Cecília |
| dc.date.Accepted.fl_str_mv | 2015-02-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2016-04-20T14:20:47Z |
| dc.date.embargoed.fl_str_mv | 2016-04-20T14:20:47Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.21/6058 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Wiley-Blackwell |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Plasmid bioprocess Escherichia coli Near infrared spectroscopy In situ monitoring |
| dc.title.fl_str_mv | In situ NIR spectroscopy monitoring of plasmid production processes effect of producing strain, medium composition and the cultivation strategy |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | BACKGROUNDWhile the pharmaceutical industry keeps an eye on plasmid DNA production for new generation gene therapies, real-time monitoring techniques for plasmid bioproduction are as yet unavailable. This work shows the possibility of in situ monitoring of plasmid production in Escherichia coli cultures using a near infrared (NIR) fiber optic probe. RESULTSPartial least squares (PLS) regression models based on the NIR spectra were developed for predicting bioprocess critical variables such as the concentrations of biomass, plasmid, carbon sources (glucose and glycerol) and acetate. In order to achieve robust models able to predict the performance of plasmid production processes, independently of the composition of the cultivation medium, cultivation strategy (batch versus fed-batch) and E. coli strain used, three strategies were adopted, using: (i) E. coliDH5 cultures conducted under different media compositions and culture strategies (batch and fed-batch); (ii) engineered E. coli strains, MG1655endArecApgi and MG1655endArecA, grown on the same medium and culture strategy; (iii) diverse E. coli strains, over batch and fed-batch cultivations and using different media compositions. PLS models showed high accuracy for predicting all variables in the three groups of cultures. CONCLUSIONNIR spectroscopy combined with PLS modeling provides a fast, inexpensive and contamination-free technique to accurately monitoring plasmid bioprocesses in real time, independently of the medium composition, cultivation strategy and the E. coli strain used. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ipl.pt/bitstreams/553f4498-f213-4444-9cc4-bb3df9a67815/download |
| funding.funder.alternateName_str_mv | FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia |
| id | ripl_c135d7ea7f9cdc8a528994ded96843fd |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.21/6058 |
| instacron_str | ipl |
| institution | Instituto Politécnico de Lisboa |
| instname_str | Instituto Politécnico de Lisboa |
| language | eng |
| network_acronym_str | ripl |
| network_name_str | Repositório Científico do Instituto Politécnico de Lisboa |
| oai_identifier_str | oai:repositorio.ipl.pt:10400.21/6058 |
| organization_str_mv | urn:organizationAcronym:ipl |
| person_str_mv | Lopes, Marta B. Gonçalves, Geisa A. L. Felício-Silva, Daniel Prather, Kristala L. J. Monteiro, Gabriel Prazeres, Duarte M. F. Calado, Cecília Calado, Cecília https://www.ciencia-id.pt/9418-E320-3177 9418-E320-3177 http://orcid.org/0000-0002-5264-9755 0000-0002-5264-9755 |
| publishDate | 2015 |
| publisher.none.fl_str_mv | Wiley-Blackwell |
| reponame_str | Repositório Científico do Instituto Politécnico de Lisboa |
| repository_id_str | urn:repositoryAcronym:ripl |
| service_str_mv | urn:repositoryAcronym:ripl |
| spelling | engWiley-Blackwellpt_PTBACKGROUNDWhile the pharmaceutical industry keeps an eye on plasmid DNA production for new generation gene therapies, real-time monitoring techniques for plasmid bioproduction are as yet unavailable. This work shows the possibility of in situ monitoring of plasmid production in Escherichia coli cultures using a near infrared (NIR) fiber optic probe. RESULTSPartial least squares (PLS) regression models based on the NIR spectra were developed for predicting bioprocess critical variables such as the concentrations of biomass, plasmid, carbon sources (glucose and glycerol) and acetate. In order to achieve robust models able to predict the performance of plasmid production processes, independently of the composition of the cultivation medium, cultivation strategy (batch versus fed-batch) and E. coli strain used, three strategies were adopted, using: (i) E. coliDH5 cultures conducted under different media compositions and culture strategies (batch and fed-batch); (ii) engineered E. coli strains, MG1655endArecApgi and MG1655endArecA, grown on the same medium and culture strategy; (iii) diverse E. coli strains, over batch and fed-batch cultivations and using different media compositions. PLS models showed high accuracy for predicting all variables in the three groups of cultures. CONCLUSIONNIR spectroscopy combined with PLS modeling provides a fast, inexpensive and contamination-free technique to accurately monitoring plasmid bioprocesses in real time, independently of the medium composition, cultivation strategy and the E. coli strain used.application/pdfpt_PTIn situ NIR spectroscopy monitoring of plasmid production processes effect of producing strain, medium composition and the cultivation strategyLopes, Marta B.Gonçalves, Geisa A. L.Felício-Silva, DanielPrather, Kristala L. J.Monteiro, GabrielPrazeres, Duarte M. F.PersonalCalado, CecíliaDSpacehttp://dspace.org/items/e8577257-c64c-4481-9b2b-940fedb360ccDSpacehttp://dspace.org/items/e8577257-c64c-4481-9b2b-940fedb360ccCaladoCecíliaCiência IDhttps://www.ciencia-id.pt9418-E320-3177ORCIDhttp://orcid.org0000-0002-5264-9755Researcher IDhttps://www.researcherid.comE-2102-2014Researcher IDhttps://www.researcherid.comE-2102-2014Scopus Author IDhttps://www.scopus.com6603163260HostingInstitutionOrganizationalRCIPLe-mailmailto:rcaap@sp.ipl.ptrcaap@sp.ipl.ptISSNIsPartOf0268-2575ISSNIsPartOf1097-4660DOIIsPartOf10.1002/jctb.44312016-04-20T14:20:47Z2015-022015-02-01T00:00:00ZHandlehttp://hdl.handle.net/10400.21/6058http://purl.org/coar/access_right/c_16ecrestricted accessPlasmid bioprocessEscherichia coliNear infrared spectroscopyIn situ monitoring822701 bytesFundação para a Ciência e a TecnologiaRATIONAL ENGINEERING OF E. COLI STRAINS AND VECTORS FOR IMPROVED MANUFACTURING OF PLASMID BIOPHARMACEUTICALSCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ipl.pt/bitstreams/553f4498-f213-4444-9cc4-bb3df9a67815/download902255261 |
| spellingShingle | In situ NIR spectroscopy monitoring of plasmid production processes effect of producing strain, medium composition and the cultivation strategy Lopes, Marta B. Plasmid bioprocess Escherichia coli Near infrared spectroscopy In situ monitoring |
| status | SINGLETON |
| subject.fl_str_mv | Plasmid bioprocess Escherichia coli Near infrared spectroscopy In situ monitoring |
| title | In situ NIR spectroscopy monitoring of plasmid production processes effect of producing strain, medium composition and the cultivation strategy |
| title_full | In situ NIR spectroscopy monitoring of plasmid production processes effect of producing strain, medium composition and the cultivation strategy |
| title_fullStr | In situ NIR spectroscopy monitoring of plasmid production processes effect of producing strain, medium composition and the cultivation strategy |
| title_full_unstemmed | In situ NIR spectroscopy monitoring of plasmid production processes effect of producing strain, medium composition and the cultivation strategy |
| title_short | In situ NIR spectroscopy monitoring of plasmid production processes effect of producing strain, medium composition and the cultivation strategy |
| title_sort | In situ NIR spectroscopy monitoring of plasmid production processes effect of producing strain, medium composition and the cultivation strategy |
| topic | Plasmid bioprocess Escherichia coli Near infrared spectroscopy In situ monitoring |
| topic_facet | Plasmid bioprocess Escherichia coli Near infrared spectroscopy In situ monitoring |
| url | http://hdl.handle.net/10400.21/6058 |
| visible | 1 |