Publicação
Fat intake interacts with polymorphisms of Caspase9, FasLigand and PPARgamma apoptotic genes in modulating Crohn's disease activity
| Resumo: | Background & aims: Crohn’s disease (CD) is a multifactorial disease where resistance to apoptosis is one major defect. Also, dietary fat intake has been shown to modulate disease activity. We aimed to explore the interaction between four single nucleotide polymorphisms (SNPs) in apoptotic genes and dietary fat intake in modulating disease activity in CD patients. Methods: Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) techniques were used to analyze Caspase9þ93C/T, FasLigand-843C/T, Peroxisome Proliferator-Activated Receptor gammaþ161C/T and Peroxisome Proliferator-Activated Receptor gamma Pro12Ala SNPs in 99 patients with CD and 116 healthy controls. Interactions between SNPs and fat intake in modulating disease activity were analyzed using regression analysis. Results: None of the polymorphisms analyzed influenced disease susceptibility and/or activity, but a high intake of total, saturated and monounsaturated fats and a higher ratio of n-6/n-3 polyunsaturated fatty acids (PUFA), was associated with a more active phenotype (p < 0.05). We observed that the detrimental effect of a high intake of total and trans fat was more marked in wild type carriers of the Caspase9þ93C/T polymorphism [O.R (95%CI) 4.64 (1.27e16.89) and O.R (95%CI) 4.84 (1.34e17.50)]. In the Peroxisome Proliferator-Activated Receptor gamma Pro12Ala SNP, we also observed that a high intake of saturated and monounsaturated fat was associated to a more active disease in wild type carriers [OR (95%CI) 4.21 (1.33e13.26) and 4.37 (1.52e12.51)]. Finally, a high intake of n-6 PUFA was associated with a more active disease in wild type carriers for the FasLigand-843C/T polymorphism [O.R (95%CI) 5.15 (1.07e24.74)]. Conclusions: To our knowledge, this is the first study to disclose a synergism between fat intake and SNPs in apoptotic genes in modulating disease activity in CD patients. |
|---|---|
| Autores principais: | Ferreira, Paula |
| Outros Autores: | Cravo, Marília; Guerreiro, Catarina Sousa; Tavares, Lourdes; Santos, Paula Moura dos; Brito, Miguel |
| Assunto: | Case-control studies Caspase 9 Crohn disease Diet Dietary fats Fas ligand protein Fatty acids Genetic predisposition to disease PPAR gamm Polymorphism, Single nucleotide Regression analysis |
| Ano: | 2010 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Científico do Instituto Politécnico de Lisboa |
| _version_ | 1866887539274547200 |
|---|---|
| author | Ferreira, Paula |
| author2 | Cravo, Marília Guerreiro, Catarina Sousa Tavares, Lourdes Santos, Paula Moura dos Brito, Miguel |
| author2_role | author author author author author |
| author_facet | Ferreira, Paula Cravo, Marília Guerreiro, Catarina Sousa Tavares, Lourdes Santos, Paula Moura dos Brito, Miguel |
| author_role | author |
| contributor_name_str_mv | RCIPL |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Ferreira, Paula\"},{\"Person.name\":\"Cravo, Marília\"},{\"Person.name\":\"Guerreiro, Catarina Sousa\"},{\"Person.name\":\"Tavares, Lourdes\"},{\"Person.name\":\"Santos, Paula Moura dos\"},{\"Person.name\":\"Brito, Miguel\",\"Person.identifier.orcid\":\"0000-0001-6394-658X\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RCIPL |
| datacite.creators.creator.creatorName.fl_str_mv | Ferreira, Paula Cravo, Marília Guerreiro, Catarina Sousa Tavares, Lourdes Santos, Paula Moura dos Brito, Miguel |
| datacite.date.Accepted.fl_str_mv | 2010-12-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2014-01-02T16:04:12Z |
| datacite.date.embargoed.fl_str_mv | 2014-01-02T16:04:12Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Case-control studies Caspase 9 Crohn disease Diet Dietary fats Fas ligand protein Fatty acids Genetic predisposition to disease PPAR gamm Polymorphism, Single nucleotide Regression analysis |
| datacite.titles.title.fl_str_mv | Fat intake interacts with polymorphisms of Caspase9, FasLigand and PPARgamma apoptotic genes in modulating Crohn's disease activity |
| dc.contributor.none.fl_str_mv | RCIPL |
| dc.creator.none.fl_str_mv | Ferreira, Paula Cravo, Marília Guerreiro, Catarina Sousa Tavares, Lourdes Santos, Paula Moura dos Brito, Miguel |
| dc.date.Accepted.fl_str_mv | 2010-12-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2014-01-02T16:04:12Z |
| dc.date.embargoed.fl_str_mv | 2014-01-02T16:04:12Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.21/3051 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Case-control studies Caspase 9 Crohn disease Diet Dietary fats Fas ligand protein Fatty acids Genetic predisposition to disease PPAR gamm Polymorphism, Single nucleotide Regression analysis |
| dc.title.fl_str_mv | Fat intake interacts with polymorphisms of Caspase9, FasLigand and PPARgamma apoptotic genes in modulating Crohn's disease activity |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Background & aims: Crohn’s disease (CD) is a multifactorial disease where resistance to apoptosis is one major defect. Also, dietary fat intake has been shown to modulate disease activity. We aimed to explore the interaction between four single nucleotide polymorphisms (SNPs) in apoptotic genes and dietary fat intake in modulating disease activity in CD patients. Methods: Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) techniques were used to analyze Caspase9þ93C/T, FasLigand-843C/T, Peroxisome Proliferator-Activated Receptor gammaþ161C/T and Peroxisome Proliferator-Activated Receptor gamma Pro12Ala SNPs in 99 patients with CD and 116 healthy controls. Interactions between SNPs and fat intake in modulating disease activity were analyzed using regression analysis. Results: None of the polymorphisms analyzed influenced disease susceptibility and/or activity, but a high intake of total, saturated and monounsaturated fats and a higher ratio of n-6/n-3 polyunsaturated fatty acids (PUFA), was associated with a more active phenotype (p < 0.05). We observed that the detrimental effect of a high intake of total and trans fat was more marked in wild type carriers of the Caspase9þ93C/T polymorphism [O.R (95%CI) 4.64 (1.27e16.89) and O.R (95%CI) 4.84 (1.34e17.50)]. In the Peroxisome Proliferator-Activated Receptor gamma Pro12Ala SNP, we also observed that a high intake of saturated and monounsaturated fat was associated to a more active disease in wild type carriers [OR (95%CI) 4.21 (1.33e13.26) and 4.37 (1.52e12.51)]. Finally, a high intake of n-6 PUFA was associated with a more active disease in wild type carriers for the FasLigand-843C/T polymorphism [O.R (95%CI) 5.15 (1.07e24.74)]. Conclusions: To our knowledge, this is the first study to disclose a synergism between fat intake and SNPs in apoptotic genes in modulating disease activity in CD patients. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ipl.pt/bitstreams/dfbe420b-998b-4a40-879f-dce4dd94f820/download |
| id | ripl_db0d2ca97d1445e245f08aa4e087fc41 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.21/3051 |
| instacron_str | ipl |
| institution | Instituto Politécnico de Lisboa |
| instname_str | Instituto Politécnico de Lisboa |
| language | eng |
| network_acronym_str | ripl |
| network_name_str | Repositório Científico do Instituto Politécnico de Lisboa |
| oai_identifier_str | oai:repositorio.ipl.pt:10400.21/3051 |
| organization_str_mv | urn:organizationAcronym:ipl |
| person_str_mv | Ferreira, Paula Cravo, Marília Guerreiro, Catarina Sousa Tavares, Lourdes Santos, Paula Moura dos Brito, Miguel Brito, Miguel https://www.ciencia-id.pt/231F-F341-7E93 231F-F341-7E93 http://orcid.org/0000-0001-6394-658X 0000-0001-6394-658X |
| publishDate | 2010 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | Repositório Científico do Instituto Politécnico de Lisboa |
| repository_id_str | urn:repositoryAcronym:ripl |
| service_str_mv | urn:repositoryAcronym:ripl |
| spelling | engElsevierporBackground & aims: Crohn’s disease (CD) is a multifactorial disease where resistance to apoptosis is one major defect. Also, dietary fat intake has been shown to modulate disease activity. We aimed to explore the interaction between four single nucleotide polymorphisms (SNPs) in apoptotic genes and dietary fat intake in modulating disease activity in CD patients. Methods: Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) techniques were used to analyze Caspase9þ93C/T, FasLigand-843C/T, Peroxisome Proliferator-Activated Receptor gammaþ161C/T and Peroxisome Proliferator-Activated Receptor gamma Pro12Ala SNPs in 99 patients with CD and 116 healthy controls. Interactions between SNPs and fat intake in modulating disease activity were analyzed using regression analysis. Results: None of the polymorphisms analyzed influenced disease susceptibility and/or activity, but a high intake of total, saturated and monounsaturated fats and a higher ratio of n-6/n-3 polyunsaturated fatty acids (PUFA), was associated with a more active phenotype (p < 0.05). We observed that the detrimental effect of a high intake of total and trans fat was more marked in wild type carriers of the Caspase9þ93C/T polymorphism [O.R (95%CI) 4.64 (1.27e16.89) and O.R (95%CI) 4.84 (1.34e17.50)]. In the Peroxisome Proliferator-Activated Receptor gamma Pro12Ala SNP, we also observed that a high intake of saturated and monounsaturated fat was associated to a more active disease in wild type carriers [OR (95%CI) 4.21 (1.33e13.26) and 4.37 (1.52e12.51)]. Finally, a high intake of n-6 PUFA was associated with a more active disease in wild type carriers for the FasLigand-843C/T polymorphism [O.R (95%CI) 5.15 (1.07e24.74)]. Conclusions: To our knowledge, this is the first study to disclose a synergism between fat intake and SNPs in apoptotic genes in modulating disease activity in CD patients.application/pdfporFat intake interacts with polymorphisms of Caspase9, FasLigand and PPARgamma apoptotic genes in modulating Crohn's disease activityFerreira, PaulaCravo, MaríliaGuerreiro, Catarina SousaTavares, LourdesSantos, Paula Moura dosPersonalBrito, MiguelDSpacehttp://dspace.org/items/4252d8e0-800c-4d67-8b13-0b711d860669DSpacehttp://dspace.org/items/4252d8e0-800c-4d67-8b13-0b711d860669BritoMiguelCiência IDhttps://www.ciencia-id.pt231F-F341-7E93ORCIDhttp://orcid.org0000-0001-6394-658XResearcher IDhttps://www.researcherid.comA-7970-2016Scopus Author IDhttps://www.scopus.com35224551000Scopus Author IDhttps://www.scopus.com57200288349HostingInstitutionOrganizationalRCIPLe-mailmailto:rcaap@sp.ipl.ptrcaap@sp.ipl.ptISSNIsPartOf1532-19832014-01-02T16:04:12Z2010-122010-12-01T00:00:00ZHandlehttp://hdl.handle.net/10400.21/3051http://purl.org/coar/access_right/c_abf2open accessCase-control studiesCaspase 9Crohn diseaseDietDietary fatsFas ligand proteinFatty acidsGenetic predisposition to diseasePPAR gammPolymorphism, Single nucleotideRegression analysis123294 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ipl.pt/bitstreams/dfbe420b-998b-4a40-879f-dce4dd94f820/downloadClinical Nutrition29819823 |
| spellingShingle | Fat intake interacts with polymorphisms of Caspase9, FasLigand and PPARgamma apoptotic genes in modulating Crohn's disease activity Ferreira, Paula Case-control studies Caspase 9 Crohn disease Diet Dietary fats Fas ligand protein Fatty acids Genetic predisposition to disease PPAR gamm Polymorphism, Single nucleotide Regression analysis |
| status | SINGLETON |
| subject.fl_str_mv | Case-control studies Caspase 9 Crohn disease Diet Dietary fats Fas ligand protein Fatty acids Genetic predisposition to disease PPAR gamm Polymorphism, Single nucleotide Regression analysis |
| title | Fat intake interacts with polymorphisms of Caspase9, FasLigand and PPARgamma apoptotic genes in modulating Crohn's disease activity |
| title_full | Fat intake interacts with polymorphisms of Caspase9, FasLigand and PPARgamma apoptotic genes in modulating Crohn's disease activity |
| title_fullStr | Fat intake interacts with polymorphisms of Caspase9, FasLigand and PPARgamma apoptotic genes in modulating Crohn's disease activity |
| title_full_unstemmed | Fat intake interacts with polymorphisms of Caspase9, FasLigand and PPARgamma apoptotic genes in modulating Crohn's disease activity |
| title_short | Fat intake interacts with polymorphisms of Caspase9, FasLigand and PPARgamma apoptotic genes in modulating Crohn's disease activity |
| title_sort | Fat intake interacts with polymorphisms of Caspase9, FasLigand and PPARgamma apoptotic genes in modulating Crohn's disease activity |
| topic | Case-control studies Caspase 9 Crohn disease Diet Dietary fats Fas ligand protein Fatty acids Genetic predisposition to disease PPAR gamm Polymorphism, Single nucleotide Regression analysis |
| topic_facet | Case-control studies Caspase 9 Crohn disease Diet Dietary fats Fas ligand protein Fatty acids Genetic predisposition to disease PPAR gamm Polymorphism, Single nucleotide Regression analysis |
| url | http://hdl.handle.net/10400.21/3051 |
| visible | 1 |