Publicação
BCG-loaded chitosan microparticles: interaction with macrophages and preliminary in vivo studies
| Resumo: | The aim of this study was to develop a novel BCG-loaded chitosan vaccine with high association efficiency which can afford efficient interaction with APC and elicit local and Th1-type-specific immune response after intranasal administration. Chitosan-suspended BCG and BCG-loaded chitosan-alginate microparticles were prepared by ionotropic gelation. Interaction with APC was evaluated by fluorescence microscopy using rBCG-GFP. Specific immune responses were evaluated following intranasal immunization of mice. Cellular uptake was approximately two-fold higher for chitosan-suspended BCG. A single dose of BCG-loaded microparticles or chitosan-suspended BCG by intranasal route improved Th1-type response compared with subcutaneous BCG. Chitosan-suspended BCG originated the highest mucosal response in the lungs by the intranasal route. These positive results indicate that the proposed approach of whole live BCG microencapsulation in chitosan-alginate for intranasal immunization was successful in allowing efficient interaction with APC while improving the cellular immune response, which is of interest for local immunization against tuberculosis. |
|---|---|
| Autores principais: | Caetano, Liliana Aranha |
| Outros Autores: | Figueiredo, Lara; Almeida, António J.; Gonçalves, L. M. |
| Assunto: | Administration, Intranasal Animals Antibodies, Bacterial BCG Vaccine Chitosan Female Humans Immunoglobulin A Immunoglobulin G Macrophages Mice Mice, Inbred BALB C Mycobacterium bovis THP-1 Cells |
| Ano: | 2017 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Instituto Politécnico de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Científico do Instituto Politécnico de Lisboa |
| _version_ | 1866887246675705856 |
|---|---|
| author | Caetano, Liliana Aranha |
| author2 | Figueiredo, Lara Almeida, António J. Gonçalves, L. M. |
| author2_role | author author author |
| author_facet | Caetano, Liliana Aranha Figueiredo, Lara Almeida, António J. Gonçalves, L. M. |
| author_role | author |
| contributor_name_str_mv | RCIPL |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Caetano, Liliana Aranha\"},{\"Person.name\":\"Figueiredo, Lara\"},{\"Person.name\":\"Almeida, António J.\"},{\"Person.name\":\"Gonçalves, L. M.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RCIPL |
| datacite.creators.creator.creatorName.fl_str_mv | Caetano, Liliana Aranha Figueiredo, Lara Almeida, António J. Gonçalves, L. M. |
| datacite.date.Accepted.fl_str_mv | 2017-04-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-01-17T11:00:26Z |
| datacite.date.embargoed.fl_str_mv | 2018-01-17T11:00:26Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Administration, Intranasal Animals Antibodies, Bacterial BCG Vaccine Chitosan Female Humans Immunoglobulin A Immunoglobulin G Macrophages Mice Mice, Inbred BALB C Mycobacterium bovis THP-1 Cells |
| datacite.titles.title.fl_str_mv | BCG-loaded chitosan microparticles: interaction with macrophages and preliminary in vivo studies |
| dc.contributor.none.fl_str_mv | RCIPL |
| dc.creator.none.fl_str_mv | Caetano, Liliana Aranha Figueiredo, Lara Almeida, António J. Gonçalves, L. M. |
| dc.date.Accepted.fl_str_mv | 2017-04-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-01-17T11:00:26Z |
| dc.date.embargoed.fl_str_mv | 2018-01-17T11:00:26Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.21/7928 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Taylor & Francis |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Administration, Intranasal Animals Antibodies, Bacterial BCG Vaccine Chitosan Female Humans Immunoglobulin A Immunoglobulin G Macrophages Mice Mice, Inbred BALB C Mycobacterium bovis THP-1 Cells |
| dc.title.fl_str_mv | BCG-loaded chitosan microparticles: interaction with macrophages and preliminary in vivo studies |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The aim of this study was to develop a novel BCG-loaded chitosan vaccine with high association efficiency which can afford efficient interaction with APC and elicit local and Th1-type-specific immune response after intranasal administration. Chitosan-suspended BCG and BCG-loaded chitosan-alginate microparticles were prepared by ionotropic gelation. Interaction with APC was evaluated by fluorescence microscopy using rBCG-GFP. Specific immune responses were evaluated following intranasal immunization of mice. Cellular uptake was approximately two-fold higher for chitosan-suspended BCG. A single dose of BCG-loaded microparticles or chitosan-suspended BCG by intranasal route improved Th1-type response compared with subcutaneous BCG. Chitosan-suspended BCG originated the highest mucosal response in the lungs by the intranasal route. These positive results indicate that the proposed approach of whole live BCG microencapsulation in chitosan-alginate for intranasal immunization was successful in allowing efficient interaction with APC while improving the cellular immune response, which is of interest for local immunization against tuberculosis. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ipl.pt/bitstreams/b65c9394-29ce-4b20-b2dd-08c382521851/download |
| id | ripl_ebb7d42441c5ab6e7377e6dd62771663 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.21/7928 |
| instacron_str | ipl |
| institution | Instituto Politécnico de Lisboa |
| instname_str | Instituto Politécnico de Lisboa |
| language | eng |
| network_acronym_str | ripl |
| network_name_str | Repositório Científico do Instituto Politécnico de Lisboa |
| oai_identifier_str | oai:repositorio.ipl.pt:10400.21/7928 |
| organization_str_mv | urn:organizationAcronym:ipl |
| person_str_mv | Caetano, Liliana Aranha Figueiredo, Lara Almeida, António J. Gonçalves, L. M. |
| publishDate | 2017 |
| publisher.none.fl_str_mv | Taylor & Francis |
| reponame_str | Repositório Científico do Instituto Politécnico de Lisboa |
| repository_id_str | urn:repositoryAcronym:ripl |
| service_str_mv | urn:repositoryAcronym:ripl |
| spelling | engTaylor & Francispt_PTThe aim of this study was to develop a novel BCG-loaded chitosan vaccine with high association efficiency which can afford efficient interaction with APC and elicit local and Th1-type-specific immune response after intranasal administration. Chitosan-suspended BCG and BCG-loaded chitosan-alginate microparticles were prepared by ionotropic gelation. Interaction with APC was evaluated by fluorescence microscopy using rBCG-GFP. Specific immune responses were evaluated following intranasal immunization of mice. Cellular uptake was approximately two-fold higher for chitosan-suspended BCG. A single dose of BCG-loaded microparticles or chitosan-suspended BCG by intranasal route improved Th1-type response compared with subcutaneous BCG. Chitosan-suspended BCG originated the highest mucosal response in the lungs by the intranasal route. These positive results indicate that the proposed approach of whole live BCG microencapsulation in chitosan-alginate for intranasal immunization was successful in allowing efficient interaction with APC while improving the cellular immune response, which is of interest for local immunization against tuberculosis.application/pdfpt_PTBCG-loaded chitosan microparticles: interaction with macrophages and preliminary in vivo studiesCaetano, Liliana AranhaFigueiredo, LaraAlmeida, António J.Gonçalves, L. M.HostingInstitutionOrganizationalRCIPLe-mailmailto:rcaap@sp.ipl.ptrcaap@sp.ipl.ptDOIIsPartOf10.1080/02652048.2017.13163252018-01-17T11:00:26Z2017-042017-04-01T00:00:00ZHandlehttp://hdl.handle.net/10400.21/7928http://purl.org/coar/access_right/c_16ecrestricted accessAdministration, IntranasalAnimalsAntibodies, BacterialBCG VaccineChitosanFemaleHumansImmunoglobulin AImmunoglobulin GMacrophagesMiceMice, Inbred BALB CMycobacterium bovisTHP-1 Cells993538 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2017-04http://creativecommons.org/licenses/by-nc-nd/4.0/http://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ipl.pt/bitstreams/b65c9394-29ce-4b20-b2dd-08c382521851/downloadJournal of Microencapsulation342203217 |
| spellingShingle | BCG-loaded chitosan microparticles: interaction with macrophages and preliminary in vivo studies Caetano, Liliana Aranha Administration, Intranasal Animals Antibodies, Bacterial BCG Vaccine Chitosan Female Humans Immunoglobulin A Immunoglobulin G Macrophages Mice Mice, Inbred BALB C Mycobacterium bovis THP-1 Cells |
| status | SINGLETON |
| subject.fl_str_mv | Administration, Intranasal Animals Antibodies, Bacterial BCG Vaccine Chitosan Female Humans Immunoglobulin A Immunoglobulin G Macrophages Mice Mice, Inbred BALB C Mycobacterium bovis THP-1 Cells |
| title | BCG-loaded chitosan microparticles: interaction with macrophages and preliminary in vivo studies |
| title_full | BCG-loaded chitosan microparticles: interaction with macrophages and preliminary in vivo studies |
| title_fullStr | BCG-loaded chitosan microparticles: interaction with macrophages and preliminary in vivo studies |
| title_full_unstemmed | BCG-loaded chitosan microparticles: interaction with macrophages and preliminary in vivo studies |
| title_short | BCG-loaded chitosan microparticles: interaction with macrophages and preliminary in vivo studies |
| title_sort | BCG-loaded chitosan microparticles: interaction with macrophages and preliminary in vivo studies |
| topic | Administration, Intranasal Animals Antibodies, Bacterial BCG Vaccine Chitosan Female Humans Immunoglobulin A Immunoglobulin G Macrophages Mice Mice, Inbred BALB C Mycobacterium bovis THP-1 Cells |
| topic_facet | Administration, Intranasal Animals Antibodies, Bacterial BCG Vaccine Chitosan Female Humans Immunoglobulin A Immunoglobulin G Macrophages Mice Mice, Inbred BALB C Mycobacterium bovis THP-1 Cells |
| url | http://hdl.handle.net/10400.21/7928 |
| visible | 1 |