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BCG-loaded chitosan microparticles: interaction with macrophages and preliminary in vivo studies

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Resumo:The aim of this study was to develop a novel BCG-loaded chitosan vaccine with high association efficiency which can afford efficient interaction with APC and elicit local and Th1-type-specific immune response after intranasal administration. Chitosan-suspended BCG and BCG-loaded chitosan-alginate microparticles were prepared by ionotropic gelation. Interaction with APC was evaluated by fluorescence microscopy using rBCG-GFP. Specific immune responses were evaluated following intranasal immunization of mice. Cellular uptake was approximately two-fold higher for chitosan-suspended BCG. A single dose of BCG-loaded microparticles or chitosan-suspended BCG by intranasal route improved Th1-type response compared with subcutaneous BCG. Chitosan-suspended BCG originated the highest mucosal response in the lungs by the intranasal route. These positive results indicate that the proposed approach of whole live BCG microencapsulation in chitosan-alginate for intranasal immunization was successful in allowing efficient interaction with APC while improving the cellular immune response, which is of interest for local immunization against tuberculosis.
Autores principais:Caetano, Liliana Aranha
Outros Autores:Figueiredo, Lara; Almeida, António J.; Gonçalves, L. M.
Assunto:Administration, Intranasal Animals Antibodies, Bacterial BCG Vaccine Chitosan Female Humans Immunoglobulin A Immunoglobulin G Macrophages Mice Mice, Inbred BALB C Mycobacterium bovis THP-1 Cells
Ano:2017
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso restrito
Instituição associada:Instituto Politécnico de Lisboa
Idioma:inglês
Origem:Repositório Científico do Instituto Politécnico de Lisboa

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