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Biological properties of phenolic compound extracts in selected Algerian honeys-The inhibition of acetylcholinesterase and alpha-glucosidase activities

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Resumo:Introduction: Honey is used in various cultures as a traditional medicine and folkloric treatment. The aim of this study was to determine the antioxidant and the anti-inflammatory activities of 31 Algerian honeys and acetylcholinesterase (AChE) and alpha-glucosidase inhibitory activities of phenolic compounds extracts of these honeys. Methods: The anti-inflammatory activity of honey was evaluated by the method of inhibition of BSA (Bovin Serum Albumin) heat-induced denaturation. The inhibition of AChE and alpha-glucosidase by the honey extracts was evaluated by in-vitro methods. Results: The highest percentage of inhibition (85.33%) of BSA denaturation was obtained with polyfloral honey (H27). Radical scavenging activity of honey samples against 1,1-diphenyl-2-picrilhydrazyl (DPPH), and 2,2'-azinobis(-3) -ethylbenzothiazoline-6-sulfonic acid (ABTS) varied from 4.41 to 83.93% and from 2.52 to 63.24%, respectively. AChE inhibitory activity is one target to prevent neurodegenerative damage in Alzheimer's disease. AChE inhibition recorded values from 20.69 to 76.04%. alpha-glucosidase inhibitors such as acarbose is preconized for the control of hyperglycemia and prevent diabetes damages. Honey extracts demonstrated a significant inhibitory effect on alpha-glucosidase activity. Moreover, the effect of Fabacece honey (H19) (IC50 = 52.20 mu g/mL) was found to be more potent than acarbose (IC50 = 204.27 mu g/mL). Correlations were observed between antioxidants and anti-inflammatory activities, AChE and alpha-glucosidase inhibitions with total phenolic compounds and flavonoids content in honey samples. Conclusion: This study showed that honey could be exploited as a potential antioxidant and anti-inflammatory agent within therapeutic medicine.
Autores principais:Zaidi, Hicham
Outros Autores:Ouchemoukh, Salim; Amessis-Ouchemoukh, Nadia; DEBBACHE-BENAIDA, NADJET; Pacheco, Rita; Serralheiro, Maria Luisa; Araujo, Maria Eduarda
Assunto:Algerian honeys Anti-inflammatory activity Antioxidant activity Acetylcholinesterase inhibition Alpha-Glucosidase inhibition Phenolic compounds
Ano:2019
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso restrito
Instituição associada:Instituto Politécnico de Lisboa
Idioma:inglês
Origem:Repositório Científico do Instituto Politécnico de Lisboa
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author Zaidi, Hicham
author2 Ouchemoukh, Salim
Amessis-Ouchemoukh, Nadia
DEBBACHE-BENAIDA, NADJET
Pacheco, Rita
Serralheiro, Maria Luisa
Araujo, Maria Eduarda
author2_role author
author
author
author
author
author
author_facet Zaidi, Hicham
Ouchemoukh, Salim
Amessis-Ouchemoukh, Nadia
DEBBACHE-BENAIDA, NADJET
Pacheco, Rita
Serralheiro, Maria Luisa
Araujo, Maria Eduarda
author_role author
contributor_name_str_mv RCIPL
country_str PT
creators_json_str [{\"Person.name\":\"Zaidi, Hicham\"},{\"Person.name\":\"Ouchemoukh, Salim\"},{\"Person.name\":\"Amessis-Ouchemoukh, Nadia\"},{\"Person.name\":\"DEBBACHE-BENAIDA, NADJET\",\"Person.identifier.orcid\":\"0000-0003-1561-7305\"},{\"Person.name\":\"Pacheco, Rita\",\"Person.identifier.orcid\":\"0000-0001-5192-3006\"},{\"Person.name\":\"Serralheiro, Maria Luisa\",\"Person.identifier.orcid\":\"0000-0001-7541-9613\"},{\"Person.name\":\"Araujo, Maria Eduarda\",\"Person.identifier.orcid\":\"0000-0002-9876-9015\"}]
datacite.contributors.contributor.contributorName.fl_str_mv RCIPL
datacite.creators.creator.creatorName.fl_str_mv Zaidi, Hicham
Ouchemoukh, Salim
Amessis-Ouchemoukh, Nadia
DEBBACHE-BENAIDA, NADJET
Pacheco, Rita
Serralheiro, Maria Luisa
Araujo, Maria Eduarda
datacite.date.Accepted.fl_str_mv 2019-01-01T00:00:00Z
datacite.date.available.fl_str_mv 2019-02-08T14:37:06Z
datacite.date.embargoed.fl_str_mv 2019-02-08T14:37:06Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_16ec
datacite.subjects.subject.fl_str_mv Algerian honeys
Anti-inflammatory activity
Antioxidant activity
Acetylcholinesterase inhibition
Alpha-Glucosidase inhibition
Phenolic compounds
datacite.titles.title.fl_str_mv Biological properties of phenolic compound extracts in selected Algerian honeys-The inhibition of acetylcholinesterase and alpha-glucosidase activities
dc.contributor.none.fl_str_mv RCIPL
dc.creator.none.fl_str_mv Zaidi, Hicham
Ouchemoukh, Salim
Amessis-Ouchemoukh, Nadia
DEBBACHE-BENAIDA, NADJET
Pacheco, Rita
Serralheiro, Maria Luisa
Araujo, Maria Eduarda
dc.date.Accepted.fl_str_mv 2019-01-01T00:00:00Z
dc.date.available.fl_str_mv 2019-02-08T14:37:06Z
dc.date.embargoed.fl_str_mv 2019-02-08T14:37:06Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://hdl.handle.net/10400.21/9474
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv Elsevier
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_16ec
dc.subject.none.fl_str_mv Algerian honeys
Anti-inflammatory activity
Antioxidant activity
Acetylcholinesterase inhibition
Alpha-Glucosidase inhibition
Phenolic compounds
dc.title.fl_str_mv Biological properties of phenolic compound extracts in selected Algerian honeys-The inhibition of acetylcholinesterase and alpha-glucosidase activities
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
description Introduction: Honey is used in various cultures as a traditional medicine and folkloric treatment. The aim of this study was to determine the antioxidant and the anti-inflammatory activities of 31 Algerian honeys and acetylcholinesterase (AChE) and alpha-glucosidase inhibitory activities of phenolic compounds extracts of these honeys. Methods: The anti-inflammatory activity of honey was evaluated by the method of inhibition of BSA (Bovin Serum Albumin) heat-induced denaturation. The inhibition of AChE and alpha-glucosidase by the honey extracts was evaluated by in-vitro methods. Results: The highest percentage of inhibition (85.33%) of BSA denaturation was obtained with polyfloral honey (H27). Radical scavenging activity of honey samples against 1,1-diphenyl-2-picrilhydrazyl (DPPH), and 2,2'-azinobis(-3) -ethylbenzothiazoline-6-sulfonic acid (ABTS) varied from 4.41 to 83.93% and from 2.52 to 63.24%, respectively. AChE inhibitory activity is one target to prevent neurodegenerative damage in Alzheimer's disease. AChE inhibition recorded values from 20.69 to 76.04%. alpha-glucosidase inhibitors such as acarbose is preconized for the control of hyperglycemia and prevent diabetes damages. Honey extracts demonstrated a significant inhibitory effect on alpha-glucosidase activity. Moreover, the effect of Fabacece honey (H19) (IC50 = 52.20 mu g/mL) was found to be more potent than acarbose (IC50 = 204.27 mu g/mL). Correlations were observed between antioxidants and anti-inflammatory activities, AChE and alpha-glucosidase inhibitions with total phenolic compounds and flavonoids content in honey samples. Conclusion: This study showed that honey could be exploited as a potential antioxidant and anti-inflammatory agent within therapeutic medicine.
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eu_rights_str_mv restrictedAccess
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fulltext.url.fl_str_mv https://repositorio.ipl.pt/bitstreams/47026846-410f-4261-bfab-e5c6f6391b14/download
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identifier.url.fl_str_mv http://hdl.handle.net/10400.21/9474
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instname_str Instituto Politécnico de Lisboa
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oai_identifier_str oai:repositorio.ipl.pt:10400.21/9474
organization_str_mv urn:organizationAcronym:ipl
person_str_mv Zaidi, Hicham
Ouchemoukh, Salim
Amessis-Ouchemoukh, Nadia
DEBBACHE-BENAIDA, NADJET
DEBBACHE-BENAIDA, NADJET
http://orcid.org/0000-0003-1561-7305
0000-0003-1561-7305
Pacheco, Rita
Pacheco, Rita
https://www.ciencia-id.pt/9F13-D310-B5A3
9F13-D310-B5A3
http://orcid.org/0000-0001-5192-3006
0000-0001-5192-3006
Serralheiro, Maria Luisa
Serralheiro, Maria Luisa
http://orcid.org/0000-0001-7541-9613
0000-0001-7541-9613
Araujo, Maria Eduarda
Araujo, Maria Eduarda
http://orcid.org/0000-0002-9876-9015
0000-0002-9876-9015
publishDate 2019
publisher.none.fl_str_mv Elsevier
reponame_str Repositório Científico do Instituto Politécnico de Lisboa
repository_id_str urn:repositoryAcronym:ripl
service_str_mv urn:repositoryAcronym:ripl
spelling engElsevierpt_PTIntroduction: Honey is used in various cultures as a traditional medicine and folkloric treatment. The aim of this study was to determine the antioxidant and the anti-inflammatory activities of 31 Algerian honeys and acetylcholinesterase (AChE) and alpha-glucosidase inhibitory activities of phenolic compounds extracts of these honeys. Methods: The anti-inflammatory activity of honey was evaluated by the method of inhibition of BSA (Bovin Serum Albumin) heat-induced denaturation. The inhibition of AChE and alpha-glucosidase by the honey extracts was evaluated by in-vitro methods. Results: The highest percentage of inhibition (85.33%) of BSA denaturation was obtained with polyfloral honey (H27). Radical scavenging activity of honey samples against 1,1-diphenyl-2-picrilhydrazyl (DPPH), and 2,2'-azinobis(-3) -ethylbenzothiazoline-6-sulfonic acid (ABTS) varied from 4.41 to 83.93% and from 2.52 to 63.24%, respectively. AChE inhibitory activity is one target to prevent neurodegenerative damage in Alzheimer's disease. AChE inhibition recorded values from 20.69 to 76.04%. alpha-glucosidase inhibitors such as acarbose is preconized for the control of hyperglycemia and prevent diabetes damages. Honey extracts demonstrated a significant inhibitory effect on alpha-glucosidase activity. Moreover, the effect of Fabacece honey (H19) (IC50 = 52.20 mu g/mL) was found to be more potent than acarbose (IC50 = 204.27 mu g/mL). Correlations were observed between antioxidants and anti-inflammatory activities, AChE and alpha-glucosidase inhibitions with total phenolic compounds and flavonoids content in honey samples. Conclusion: This study showed that honey could be exploited as a potential antioxidant and anti-inflammatory agent within therapeutic medicine.application/pdfpt_PTBiological properties of phenolic compound extracts in selected Algerian honeys-The inhibition of acetylcholinesterase and alpha-glucosidase activitiesZaidi, HichamOuchemoukh, SalimAmessis-Ouchemoukh, NadiaPersonalDEBBACHE-BENAIDA, NADJETDSpacehttp://dspace.org/items/8f0da1de-6e78-4f94-a98a-46f126fea243DSpacehttp://dspace.org/items/8f0da1de-6e78-4f94-a98a-46f126fea243DEBBACHE-BENAIDANADJETORCIDhttp://orcid.org0000-0003-1561-7305PersonalPacheco, RitaDSpacehttp://dspace.org/items/d6907c0a-3f8f-431c-acf2-0b257692f0d4DSpacehttp://dspace.org/items/d6907c0a-3f8f-431c-acf2-0b257692f0d4PachecoRitaCiência IDhttps://www.ciencia-id.pt9F13-D310-B5A3ORCIDhttp://orcid.org0000-0001-5192-3006Researcher IDhttps://www.researcherid.comC-3062-2012Scopus Author IDhttps://www.scopus.com8853708300PersonalSerralheiro, Maria LuisaDSpacehttp://dspace.org/items/88895442-2a46-4878-a83f-435152a9cbb4DSpacehttp://dspace.org/items/88895442-2a46-4878-a83f-435152a9cbb4SerralheiroMaria LuisaORCIDhttp://orcid.org0000-0001-7541-9613Researcher IDhttps://www.researcherid.comE-3407-2012Scopus Author IDhttps://www.scopus.com6602688573PersonalAraujo, Maria EduardaDSpacehttp://dspace.org/items/ca23020f-df9d-4134-86d2-71dac7a97c1eDSpacehttp://dspace.org/items/ca23020f-df9d-4134-86d2-71dac7a97c1eAraujoMaria EduardaORCIDhttp://orcid.org0000-0002-9876-9015Researcher IDhttps://www.researcherid.comE-6528-2012Scopus Author IDhttps://www.scopus.com7202962614HostingInstitutionOrganizationalRCIPLe-mailmailto:rcaap@sp.ipl.ptrcaap@sp.ipl.ptISSNIsPartOf1876-3820ISSNIsPartOf1876-3839DOIIsPartOf10.1016/j.eujim.2018.11.0082019-02-08T14:37:06Z2019-012019-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.21/9474http://purl.org/coar/access_right/c_16ecrestricted accessAlgerian honeysAnti-inflammatory activityAntioxidant activityAcetylcholinesterase inhibitionAlpha-Glucosidase inhibitionPhenolic compounds643529 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ipl.pt/bitstreams/47026846-410f-4261-bfab-e5c6f6391b14/downloadEuropean Journal of Integrative Medicine257784
spellingShingle Biological properties of phenolic compound extracts in selected Algerian honeys-The inhibition of acetylcholinesterase and alpha-glucosidase activities
Zaidi, Hicham
Algerian honeys
Anti-inflammatory activity
Antioxidant activity
Acetylcholinesterase inhibition
Alpha-Glucosidase inhibition
Phenolic compounds
subject.fl_str_mv Algerian honeys
Anti-inflammatory activity
Antioxidant activity
Acetylcholinesterase inhibition
Alpha-Glucosidase inhibition
Phenolic compounds
title Biological properties of phenolic compound extracts in selected Algerian honeys-The inhibition of acetylcholinesterase and alpha-glucosidase activities
title_full Biological properties of phenolic compound extracts in selected Algerian honeys-The inhibition of acetylcholinesterase and alpha-glucosidase activities
title_fullStr Biological properties of phenolic compound extracts in selected Algerian honeys-The inhibition of acetylcholinesterase and alpha-glucosidase activities
title_full_unstemmed Biological properties of phenolic compound extracts in selected Algerian honeys-The inhibition of acetylcholinesterase and alpha-glucosidase activities
title_short Biological properties of phenolic compound extracts in selected Algerian honeys-The inhibition of acetylcholinesterase and alpha-glucosidase activities
title_sort Biological properties of phenolic compound extracts in selected Algerian honeys-The inhibition of acetylcholinesterase and alpha-glucosidase activities
topic Algerian honeys
Anti-inflammatory activity
Antioxidant activity
Acetylcholinesterase inhibition
Alpha-Glucosidase inhibition
Phenolic compounds
topic_facet Algerian honeys
Anti-inflammatory activity
Antioxidant activity
Acetylcholinesterase inhibition
Alpha-Glucosidase inhibition
Phenolic compounds
url http://hdl.handle.net/10400.21/9474
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