Publication
Comparison of the bacterial composition of two commercial composts with different physicochemical, stability and maturity properties
| Summary: | Previously, two municipal solid waste commercial composts (MSW1 and MSW2) were characterized. Although sharing the same type of raw material, most of their physicochemical, stability and maturity properties differed. The present study aimed to characterize them at a microbiological level, and to infer on possible relationships between the composts properties and the structure of their bacterial communities. Both the 16S rRNA gene-based PCR-DGGE profiling and 454-pyrosequencing technology showed that the structure of the bacterial communities of these composts was distinct. The bacterial community of MSW1 was more diverse than that of MSW2. Multivariate analyses revealed that the high electrical conductivity, Cu content as well as the low phytotoxity of compost MSW1, when compared to MSW2, contributed most to shape its bacterial community structure. Indeed, high abundance of halophilic (Halomonadaceae and Brevibacteriaceae) and metal resistant organisms (Brevibacteriaceae and Bacillaceae) were found in MSW1. In addition, Pseudonocardiaceae, Streptomycetaceae, Bacillaceae, and Brevibacteriaceae may have contributed to the high humic-like acids content and low phytotoxicity of MSW1. In contrast, the high organic matter content and the high density of the cultivable fungi population were the parameters most correlated with the structure of the bacterial community of compost MSW2, dominated by Corynebacteriaceae and mainly Aerococcaceae, taxonomic groups not commonly found in composts. |
|---|---|
| Main Authors: | Silva, Elisabete |
| Other Authors: | Lopes, Ana; Cunha-Queda, Ana; Nunes, Olga |
| Subject: | Bacterial community Diversity DGGE profiling 454-pyrosequencing Multivariate analysis |
| Year: | 2016 |
| Country: | Portugal |
| Document type: | article |
| Access type: | restricted access |
| Associated institution: | Instituto Politécnico de Viseu |
| Language: | English |
| Origin: | Repositório Científico do Instituto Politécnico de Viseu |
| _version_ | 1868350928560586752 |
|---|---|
| author | Silva, Elisabete |
| author2 | Lopes, Ana Cunha-Queda, Ana Nunes, Olga |
| author2_role | author author author |
| author_facet | Silva, Elisabete Lopes, Ana Cunha-Queda, Ana Nunes, Olga |
| author_role | author |
| contributor_name_str_mv | Instituto Politécnico de Viseu |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Silva, Elisabete\"},{\"Person.name\":\"Lopes, Ana\"},{\"Person.name\":\"Cunha-Queda, Ana\"},{\"Person.name\":\"Nunes, Olga\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Instituto Politécnico de Viseu |
| datacite.creators.creator.creatorName.fl_str_mv | Silva, Elisabete Lopes, Ana Cunha-Queda, Ana Nunes, Olga |
| datacite.date.Accepted.fl_str_mv | 2016-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2016-04-05T16:00:48Z |
| datacite.date.embargoed.fl_str_mv | 2016-04-05T16:00:48Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Bacterial community Diversity DGGE profiling 454-pyrosequencing Multivariate analysis |
| datacite.titles.title.fl_str_mv | Comparison of the bacterial composition of two commercial composts with different physicochemical, stability and maturity properties |
| dc.contributor.none.fl_str_mv | Instituto Politécnico de Viseu |
| dc.creator.none.fl_str_mv | Silva, Elisabete Lopes, Ana Cunha-Queda, Ana Nunes, Olga |
| dc.date.Accepted.fl_str_mv | 2016-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2016-04-05T16:00:48Z |
| dc.date.embargoed.fl_str_mv | 2016-04-05T16:00:48Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.19/3139 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Bacterial community Diversity DGGE profiling 454-pyrosequencing Multivariate analysis |
| dc.title.fl_str_mv | Comparison of the bacterial composition of two commercial composts with different physicochemical, stability and maturity properties |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Previously, two municipal solid waste commercial composts (MSW1 and MSW2) were characterized. Although sharing the same type of raw material, most of their physicochemical, stability and maturity properties differed. The present study aimed to characterize them at a microbiological level, and to infer on possible relationships between the composts properties and the structure of their bacterial communities. Both the 16S rRNA gene-based PCR-DGGE profiling and 454-pyrosequencing technology showed that the structure of the bacterial communities of these composts was distinct. The bacterial community of MSW1 was more diverse than that of MSW2. Multivariate analyses revealed that the high electrical conductivity, Cu content as well as the low phytotoxity of compost MSW1, when compared to MSW2, contributed most to shape its bacterial community structure. Indeed, high abundance of halophilic (Halomonadaceae and Brevibacteriaceae) and metal resistant organisms (Brevibacteriaceae and Bacillaceae) were found in MSW1. In addition, Pseudonocardiaceae, Streptomycetaceae, Bacillaceae, and Brevibacteriaceae may have contributed to the high humic-like acids content and low phytotoxicity of MSW1. In contrast, the high organic matter content and the high density of the cultivable fungi population were the parameters most correlated with the structure of the bacterial community of compost MSW2, dominated by Corynebacteriaceae and mainly Aerococcaceae, taxonomic groups not commonly found in composts. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ipv.pt/bitstreams/53c91035-f168-4694-8d5e-1cddae0c019d/download |
| id | ripv_06f36eba1cdcadcec8d8b18e00d4e65e |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.19/3139 |
| instacron_str | ripv |
| institution | Instituto Politécnico de Viseu |
| instname_str | Instituto Politécnico de Viseu |
| language | eng |
| network_acronym_str | ripv |
| network_name_str | Repositório Científico do Instituto Politécnico de Viseu |
| oai_identifier_str | oai:repositorio.ipv.pt:10400.19/3139 |
| organization_str_mv | urn:organizationAcronym:ripv |
| person_str_mv | Silva, Elisabete Lopes, Ana Cunha-Queda, Ana Nunes, Olga |
| publishDate | 2016 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | Repositório Científico do Instituto Politécnico de Viseu |
| repository_id_str | urn:repositoryAcronym:ripv |
| service_str_mv | urn:repositoryAcronym:ripv |
| spelling | engElsevierpt_PTPreviously, two municipal solid waste commercial composts (MSW1 and MSW2) were characterized. Although sharing the same type of raw material, most of their physicochemical, stability and maturity properties differed. The present study aimed to characterize them at a microbiological level, and to infer on possible relationships between the composts properties and the structure of their bacterial communities. Both the 16S rRNA gene-based PCR-DGGE profiling and 454-pyrosequencing technology showed that the structure of the bacterial communities of these composts was distinct. The bacterial community of MSW1 was more diverse than that of MSW2. Multivariate analyses revealed that the high electrical conductivity, Cu content as well as the low phytotoxity of compost MSW1, when compared to MSW2, contributed most to shape its bacterial community structure. Indeed, high abundance of halophilic (Halomonadaceae and Brevibacteriaceae) and metal resistant organisms (Brevibacteriaceae and Bacillaceae) were found in MSW1. In addition, Pseudonocardiaceae, Streptomycetaceae, Bacillaceae, and Brevibacteriaceae may have contributed to the high humic-like acids content and low phytotoxicity of MSW1. In contrast, the high organic matter content and the high density of the cultivable fungi population were the parameters most correlated with the structure of the bacterial community of compost MSW2, dominated by Corynebacteriaceae and mainly Aerococcaceae, taxonomic groups not commonly found in composts.application/pdfpt_PTComparison of the bacterial composition of two commercial composts with different physicochemical, stability and maturity propertiesSilva, ElisabeteLopes, AnaCunha-Queda, AnaNunes, OlgaHostingInstitutionOrganizationalInstituto Politécnico de Viseue-mailmailto:repositorio@sc.ipv.ptrepositorio@sc.ipv.pt2016-04-05T16:00:48Z20162016-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.19/3139http://purl.org/coar/access_right/c_16ecrestricted accessBacterial communityDiversityDGGE profiling454-pyrosequencingMultivariate analysis3877951 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ipv.pt/bitstreams/53c91035-f168-4694-8d5e-1cddae0c019d/downloadWaste management2030 |
| spellingShingle | Comparison of the bacterial composition of two commercial composts with different physicochemical, stability and maturity properties Silva, Elisabete Bacterial community Diversity DGGE profiling 454-pyrosequencing Multivariate analysis |
| status | SINGLETON |
| subject.fl_str_mv | Bacterial community Diversity DGGE profiling 454-pyrosequencing Multivariate analysis |
| title | Comparison of the bacterial composition of two commercial composts with different physicochemical, stability and maturity properties |
| title_full | Comparison of the bacterial composition of two commercial composts with different physicochemical, stability and maturity properties |
| title_fullStr | Comparison of the bacterial composition of two commercial composts with different physicochemical, stability and maturity properties |
| title_full_unstemmed | Comparison of the bacterial composition of two commercial composts with different physicochemical, stability and maturity properties |
| title_short | Comparison of the bacterial composition of two commercial composts with different physicochemical, stability and maturity properties |
| title_sort | Comparison of the bacterial composition of two commercial composts with different physicochemical, stability and maturity properties |
| topic | Bacterial community Diversity DGGE profiling 454-pyrosequencing Multivariate analysis |
| topic_facet | Bacterial community Diversity DGGE profiling 454-pyrosequencing Multivariate analysis |
| url | http://hdl.handle.net/10400.19/3139 |
| visible | 1 |