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First Detection of Borrelia burgdorferi sensu lato DNA in Serum of the Wild Boar (Sus scrofa) in Northern Portugal by Nested- PCR

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Resumo:Lyme borreliosis is the most common tick-borne zoonosis in the northern hemisphere. Several vertebrates are crucial in the epidemiological cycle of Borrelia burgdorferi sensu lato, but the role of wild boar as a reservoir is still unknown. Sera were collected from 90 wild boars shot in the Trás-os-Montes region, Northern Portugal (hunting season 2011/2012). In this study, Borrelia DNA was detected for the first time by nested-PCR in three different sera, suggesting that the wild boar may be a potential reservoir for this spirochete. Sequencing results show 100% similarity with Borrelia afzelii. Further studies are needed to evaluate the public health risks associated with boar hunting.
Autores principais:Faria, Ana Sofia
Outros Autores:Paiva-Cardoso, Maria das Neves; Nunes, Mónica; Carreira, Teresa; Vale-Gonçalves, Hélia; Veloso, Octávia; Coelho, Catarina; Cabral, João; Vieira-Pinto, Madalena; Vieira, Maria
Assunto:Borrelia burgdorferi sensu lato Wild boar PCR Fla gene Northern Portugal Lyme borreliosis Zoonosis One Health
Ano:2015
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso restrito
Instituição associada:Instituto Politécnico de Viseu
Idioma:inglês
Origem:Repositório Científico do Instituto Politécnico de Viseu
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author Faria, Ana Sofia
author2 Paiva-Cardoso, Maria das Neves
Nunes, Mónica
Carreira, Teresa
Vale-Gonçalves, Hélia
Veloso, Octávia
Coelho, Catarina
Cabral, João
Vieira-Pinto, Madalena
Vieira, Maria
author2_role author
author
author
author
author
author
author
author
author
author_facet Faria, Ana Sofia
Paiva-Cardoso, Maria das Neves
Nunes, Mónica
Carreira, Teresa
Vale-Gonçalves, Hélia
Veloso, Octávia
Coelho, Catarina
Cabral, João
Vieira-Pinto, Madalena
Vieira, Maria
author_role author
contributor_name_str_mv Instituto Politécnico de Viseu
country_str PT
creators_json_txt [{\"Person.name\":\"Faria, Ana Sofia\"},{\"Person.name\":\"Paiva-Cardoso, Maria das Neves\"},{\"Person.name\":\"Nunes, Mónica\"},{\"Person.name\":\"Carreira, Teresa\"},{\"Person.name\":\"Vale-Gonçalves, Hélia\"},{\"Person.name\":\"Veloso, Octávia\"},{\"Person.name\":\"Coelho, Catarina\"},{\"Person.name\":\"Cabral, João\"},{\"Person.name\":\"Vieira-Pinto, Madalena\"},{\"Person.name\":\"Vieira, Maria\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Instituto Politécnico de Viseu
datacite.creators.creator.creatorName.fl_str_mv Faria, Ana Sofia
Paiva-Cardoso, Maria das Neves
Nunes, Mónica
Carreira, Teresa
Vale-Gonçalves, Hélia
Veloso, Octávia
Coelho, Catarina
Cabral, João
Vieira-Pinto, Madalena
Vieira, Maria
datacite.date.Accepted.fl_str_mv 2015-03-01T00:00:00Z
datacite.date.available.fl_str_mv 2017-01-31T16:17:40Z
datacite.date.embargoed.fl_str_mv 2017-01-31T16:17:40Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_16ec
datacite.subjects.subject.fl_str_mv Borrelia burgdorferi sensu lato
Wild boar
PCR
Fla gene
Northern Portugal
Lyme borreliosis
Zoonosis
One Health
datacite.titles.title.fl_str_mv First Detection of Borrelia burgdorferi sensu lato DNA in Serum of the Wild Boar (Sus scrofa) in Northern Portugal by Nested- PCR
dc.contributor.none.fl_str_mv Instituto Politécnico de Viseu
dc.creator.none.fl_str_mv Faria, Ana Sofia
Paiva-Cardoso, Maria das Neves
Nunes, Mónica
Carreira, Teresa
Vale-Gonçalves, Hélia
Veloso, Octávia
Coelho, Catarina
Cabral, João
Vieira-Pinto, Madalena
Vieira, Maria
dc.date.Accepted.fl_str_mv 2015-03-01T00:00:00Z
dc.date.available.fl_str_mv 2017-01-31T16:17:40Z
dc.date.embargoed.fl_str_mv 2017-01-31T16:17:40Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://hdl.handle.net/10400.19/4290
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv SpringerLink
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_16ec
dc.subject.none.fl_str_mv Borrelia burgdorferi sensu lato
Wild boar
PCR
Fla gene
Northern Portugal
Lyme borreliosis
Zoonosis
One Health
dc.title.fl_str_mv First Detection of Borrelia burgdorferi sensu lato DNA in Serum of the Wild Boar (Sus scrofa) in Northern Portugal by Nested- PCR
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
description Lyme borreliosis is the most common tick-borne zoonosis in the northern hemisphere. Several vertebrates are crucial in the epidemiological cycle of Borrelia burgdorferi sensu lato, but the role of wild boar as a reservoir is still unknown. Sera were collected from 90 wild boars shot in the Trás-os-Montes region, Northern Portugal (hunting season 2011/2012). In this study, Borrelia DNA was detected for the first time by nested-PCR in three different sera, suggesting that the wild boar may be a potential reservoir for this spirochete. Sequencing results show 100% similarity with Borrelia afzelii. Further studies are needed to evaluate the public health risks associated with boar hunting.
dirty 0
eu_rights_str_mv restrictedAccess
format article
fulltext.url.fl_str_mv https://repositorio.ipv.pt/bitstreams/96a33a94-9cbd-4fe4-9b9b-a190099daeab/download
id ripv_a17e5da7070b60fe17ff4b00a3837b32
identifier.url.fl_str_mv http://hdl.handle.net/10400.19/4290
instacron_str ripv
institution Instituto Politécnico de Viseu
instname_str Instituto Politécnico de Viseu
language eng
network_acronym_str ripv
network_name_str Repositório Científico do Instituto Politécnico de Viseu
oai_identifier_str oai:repositorio.ipv.pt:10400.19/4290
organization_str_mv urn:organizationAcronym:ripv
person_str_mv Faria, Ana Sofia
Paiva-Cardoso, Maria das Neves
Nunes, Mónica
Carreira, Teresa
Vale-Gonçalves, Hélia
Veloso, Octávia
Coelho, Catarina
Cabral, João
Vieira-Pinto, Madalena
Vieira, Maria
publishDate 2015
publisher.none.fl_str_mv SpringerLink
reponame_str Repositório Científico do Instituto Politécnico de Viseu
repository_id_str urn:repositoryAcronym:ripv
service_str_mv urn:repositoryAcronym:ripv
spelling engSpringerLinkpt_PTLyme borreliosis is the most common tick-borne zoonosis in the northern hemisphere. Several vertebrates are crucial in the epidemiological cycle of Borrelia burgdorferi sensu lato, but the role of wild boar as a reservoir is still unknown. Sera were collected from 90 wild boars shot in the Trás-os-Montes region, Northern Portugal (hunting season 2011/2012). In this study, Borrelia DNA was detected for the first time by nested-PCR in three different sera, suggesting that the wild boar may be a potential reservoir for this spirochete. Sequencing results show 100% similarity with Borrelia afzelii. Further studies are needed to evaluate the public health risks associated with boar hunting.application/pdfpt_PTFirst Detection of Borrelia burgdorferi sensu lato DNA in Serum of the Wild Boar (Sus scrofa) in Northern Portugal by Nested- PCRFaria, Ana SofiaPaiva-Cardoso, Maria das NevesNunes, MónicaCarreira, TeresaVale-Gonçalves, HéliaVeloso, OctáviaCoelho, CatarinaCabral, JoãoVieira-Pinto, MadalenaVieira, MariaHostingInstitutionOrganizationalInstituto Politécnico de Viseue-mailmailto:repositorio@sc.ipv.ptrepositorio@sc.ipv.ptISSNIsPartOf1612-9210DOIIsPartOf10.1007/s10393-014-0973-42017-01-31T16:17:40Z2015-032015-03-01T00:00:00ZHandlehttp://hdl.handle.net/10400.19/4290http://purl.org/coar/access_right/c_16ecrestricted accessBorrelia burgdorferi sensu latoWild boarPCRFla geneNorthern PortugalLyme borreliosisZoonosisOne Health9656 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ipv.pt/bitstreams/96a33a94-9cbd-4fe4-9b9b-a190099daeab/downloadEcoHealth12183187
spellingShingle First Detection of Borrelia burgdorferi sensu lato DNA in Serum of the Wild Boar (Sus scrofa) in Northern Portugal by Nested- PCR
Faria, Ana Sofia
Borrelia burgdorferi sensu lato
Wild boar
PCR
Fla gene
Northern Portugal
Lyme borreliosis
Zoonosis
One Health
status SINGLETON
subject.fl_str_mv Borrelia burgdorferi sensu lato
Wild boar
PCR
Fla gene
Northern Portugal
Lyme borreliosis
Zoonosis
One Health
title First Detection of Borrelia burgdorferi sensu lato DNA in Serum of the Wild Boar (Sus scrofa) in Northern Portugal by Nested- PCR
title_full First Detection of Borrelia burgdorferi sensu lato DNA in Serum of the Wild Boar (Sus scrofa) in Northern Portugal by Nested- PCR
title_fullStr First Detection of Borrelia burgdorferi sensu lato DNA in Serum of the Wild Boar (Sus scrofa) in Northern Portugal by Nested- PCR
title_full_unstemmed First Detection of Borrelia burgdorferi sensu lato DNA in Serum of the Wild Boar (Sus scrofa) in Northern Portugal by Nested- PCR
title_short First Detection of Borrelia burgdorferi sensu lato DNA in Serum of the Wild Boar (Sus scrofa) in Northern Portugal by Nested- PCR
title_sort First Detection of Borrelia burgdorferi sensu lato DNA in Serum of the Wild Boar (Sus scrofa) in Northern Portugal by Nested- PCR
topic Borrelia burgdorferi sensu lato
Wild boar
PCR
Fla gene
Northern Portugal
Lyme borreliosis
Zoonosis
One Health
topic_facet Borrelia burgdorferi sensu lato
Wild boar
PCR
Fla gene
Northern Portugal
Lyme borreliosis
Zoonosis
One Health
url http://hdl.handle.net/10400.19/4290
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