Publicação
Histological classification of urothelial lesions chemically-induced in the male rat model
| Resumo: | Objective: Bladder cancer is one of the most frequent of the urinary tract. Urinary bladder tumours induced by the N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) are similar to those observed in humans. This work aimed to classify, by histology, the urinary bladder tumours BBN-induced in a rat model. Methods: Procedures were made in accordance with the Directive 2010/63/EU. Eleven Wistar male rats with five weeks of age were used. BBN was administered in drinking water at a concentration of 0.05% water during the first 20 weeks of the protocol. Thirty-five weeks after the start of the protocol, animals were humanely sacrificed by the overdose of pentobarbital sodium. Results: No animals died during the protocol. In the histological analysis of the urinary bladders were identified the following lesions: simple hyperplasia (81.8%), nodular hyperplasia (54.5%), papillary hyperplasia (14%), papilloma (45.4%), papillary neoplasm of low malignant potential (36.4%), low-grade papillary carcinoma (90.9%), high-grade papillary carcinoma (72.7%), invasive carcinoma (14%) and epidermoid metaplasia with keratinization (45.4%). Conclusion: Our team has a vast experience on the bladder cancer animal models; however this is the first protocol in male rats. We concluded that the lesions identified are similar to those observed in Humans. |
|---|---|
| Autores principais: | Faustino-Rocha, Ana Isabel |
| Outros Autores: | Pinto, Carla; Vala, Helena; Ferreira, Rita; Oliveira, Paula Alexandra |
| Assunto: | Bladder cancer Urinary bladder tumours N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) |
| Ano: | 2014 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Instituto Politécnico de Viseu |
| Idioma: | inglês |
| Origem: | Repositório Científico do Instituto Politécnico de Viseu |
| _version_ | 1868350931013206016 |
|---|---|
| author | Faustino-Rocha, Ana Isabel |
| author2 | Pinto, Carla Vala, Helena Ferreira, Rita Oliveira, Paula Alexandra |
| author2_role | author author author author |
| author_facet | Faustino-Rocha, Ana Isabel Pinto, Carla Vala, Helena Ferreira, Rita Oliveira, Paula Alexandra |
| author_role | author |
| contributor_name_str_mv | Instituto Politécnico de Viseu |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Faustino-Rocha, Ana Isabel\"},{\"Person.name\":\"Pinto, Carla\"},{\"Person.name\":\"Vala, Helena\",\"Person.identifier.orcid\":\"0000-0001-6829-4867\"},{\"Person.name\":\"Ferreira, Rita\"},{\"Person.name\":\"Oliveira, Paula Alexandra\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Instituto Politécnico de Viseu |
| datacite.creators.creator.creatorName.fl_str_mv | Faustino-Rocha, Ana Isabel Pinto, Carla Vala, Helena Ferreira, Rita Oliveira, Paula Alexandra |
| datacite.date.Accepted.fl_str_mv | 2014-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2015-03-23T11:22:30Z |
| datacite.date.embargoed.fl_str_mv | 2015-03-23T11:22:30Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Bladder cancer Urinary bladder tumours N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) |
| datacite.titles.title.fl_str_mv | Histological classification of urothelial lesions chemically-induced in the male rat model |
| dc.contributor.none.fl_str_mv | Instituto Politécnico de Viseu |
| dc.creator.none.fl_str_mv | Faustino-Rocha, Ana Isabel Pinto, Carla Vala, Helena Ferreira, Rita Oliveira, Paula Alexandra |
| dc.date.Accepted.fl_str_mv | 2014-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2015-03-23T11:22:30Z |
| dc.date.embargoed.fl_str_mv | 2015-03-23T11:22:30Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.19/2730 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Bladder cancer Urinary bladder tumours N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) |
| dc.title.fl_str_mv | Histological classification of urothelial lesions chemically-induced in the male rat model |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Objective: Bladder cancer is one of the most frequent of the urinary tract. Urinary bladder tumours induced by the N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) are similar to those observed in humans. This work aimed to classify, by histology, the urinary bladder tumours BBN-induced in a rat model. Methods: Procedures were made in accordance with the Directive 2010/63/EU. Eleven Wistar male rats with five weeks of age were used. BBN was administered in drinking water at a concentration of 0.05% water during the first 20 weeks of the protocol. Thirty-five weeks after the start of the protocol, animals were humanely sacrificed by the overdose of pentobarbital sodium. Results: No animals died during the protocol. In the histological analysis of the urinary bladders were identified the following lesions: simple hyperplasia (81.8%), nodular hyperplasia (54.5%), papillary hyperplasia (14%), papilloma (45.4%), papillary neoplasm of low malignant potential (36.4%), low-grade papillary carcinoma (90.9%), high-grade papillary carcinoma (72.7%), invasive carcinoma (14%) and epidermoid metaplasia with keratinization (45.4%). Conclusion: Our team has a vast experience on the bladder cancer animal models; however this is the first protocol in male rats. We concluded that the lesions identified are similar to those observed in Humans. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | conferenceObject |
| fulltext.url.fl_str_mv | https://repositorio.ipv.pt/bitstreams/5daa7dce-b140-4dc0-a890-f866d37af1ed/download |
| id | ripv_f6ad4f28fa8d102d135742200b0ee3c8 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.19/2730 |
| instacron_str | ripv |
| institution | Instituto Politécnico de Viseu |
| instname_str | Instituto Politécnico de Viseu |
| language | eng |
| network_acronym_str | ripv |
| network_name_str | Repositório Científico do Instituto Politécnico de Viseu |
| oai_identifier_str | oai:repositorio.ipv.pt:10400.19/2730 |
| organization_str_mv | urn:organizationAcronym:ripv |
| person_str_mv | Faustino-Rocha, Ana Isabel Pinto, Carla Vala, Helena Vala, Helena https://www.ciencia-id.pt/7A1E-E85E-FFA4 7A1E-E85E-FFA4 http://orcid.org/0000-0001-6829-4867 0000-0001-6829-4867 Ferreira, Rita Oliveira, Paula Alexandra |
| publishDate | 2014 |
| reponame_str | Repositório Científico do Instituto Politécnico de Viseu |
| repository_id_str | urn:repositoryAcronym:ripv |
| service_str_mv | urn:repositoryAcronym:ripv |
| spelling | engporObjective: Bladder cancer is one of the most frequent of the urinary tract. Urinary bladder tumours induced by the N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) are similar to those observed in humans. This work aimed to classify, by histology, the urinary bladder tumours BBN-induced in a rat model. Methods: Procedures were made in accordance with the Directive 2010/63/EU. Eleven Wistar male rats with five weeks of age were used. BBN was administered in drinking water at a concentration of 0.05% water during the first 20 weeks of the protocol. Thirty-five weeks after the start of the protocol, animals were humanely sacrificed by the overdose of pentobarbital sodium. Results: No animals died during the protocol. In the histological analysis of the urinary bladders were identified the following lesions: simple hyperplasia (81.8%), nodular hyperplasia (54.5%), papillary hyperplasia (14%), papilloma (45.4%), papillary neoplasm of low malignant potential (36.4%), low-grade papillary carcinoma (90.9%), high-grade papillary carcinoma (72.7%), invasive carcinoma (14%) and epidermoid metaplasia with keratinization (45.4%). Conclusion: Our team has a vast experience on the bladder cancer animal models; however this is the first protocol in male rats. We concluded that the lesions identified are similar to those observed in Humans.application/pdfporHistological classification of urothelial lesions chemically-induced in the male rat modelFaustino-Rocha, Ana IsabelPinto, CarlaPersonalVala, HelenaDSpacehttp://dspace.org/items/cdc3d2e2-df06-40ed-8900-1ecbc8a06c8aDSpacehttp://dspace.org/items/cdc3d2e2-df06-40ed-8900-1ecbc8a06c8aVala CorreiaHelena MariaCiência IDhttps://www.ciencia-id.pt7A1E-E85E-FFA4ORCIDhttp://orcid.org0000-0001-6829-4867Ferreira, RitaOliveira, Paula AlexandraHostingInstitutionOrganizationalInstituto Politécnico de Viseue-mailmailto:repositorio@sc.ipv.ptrepositorio@sc.ipv.pt2015-03-23T11:22:30Z20142014-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.19/2730http://purl.org/coar/access_right/c_16ecrestricted accessBladder cancerUrinary bladder tumoursN-butyl-N-(4-hydroxybutyl) nitrosamine (BBN)1767174 bytesother research producthttp://purl.org/coar/resource_type/c_c94fconference objecthttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ipv.pt/bitstreams/5daa7dce-b140-4dc0-a890-f866d37af1ed/downloadVirchows Arch465 (Supll I) |
| spellingShingle | Histological classification of urothelial lesions chemically-induced in the male rat model Faustino-Rocha, Ana Isabel Bladder cancer Urinary bladder tumours N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) |
| status | SINGLETON |
| subject.fl_str_mv | Bladder cancer Urinary bladder tumours N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) |
| title | Histological classification of urothelial lesions chemically-induced in the male rat model |
| title_full | Histological classification of urothelial lesions chemically-induced in the male rat model |
| title_fullStr | Histological classification of urothelial lesions chemically-induced in the male rat model |
| title_full_unstemmed | Histological classification of urothelial lesions chemically-induced in the male rat model |
| title_short | Histological classification of urothelial lesions chemically-induced in the male rat model |
| title_sort | Histological classification of urothelial lesions chemically-induced in the male rat model |
| topic | Bladder cancer Urinary bladder tumours N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) |
| topic_facet | Bladder cancer Urinary bladder tumours N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) |
| url | http://hdl.handle.net/10400.19/2730 |
| visible | 1 |