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Histological classification of urothelial lesions chemically-induced in the male rat model

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Resumo:Objective: Bladder cancer is one of the most frequent of the urinary tract. Urinary bladder tumours induced by the N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) are similar to those observed in humans. This work aimed to classify, by histology, the urinary bladder tumours BBN-induced in a rat model. Methods: Procedures were made in accordance with the Directive 2010/63/EU. Eleven Wistar male rats with five weeks of age were used. BBN was administered in drinking water at a concentration of 0.05% water during the first 20 weeks of the protocol. Thirty-five weeks after the start of the protocol, animals were humanely sacrificed by the overdose of pentobarbital sodium. Results: No animals died during the protocol. In the histological analysis of the urinary bladders were identified the following lesions: simple hyperplasia (81.8%), nodular hyperplasia (54.5%), papillary hyperplasia (14%), papilloma (45.4%), papillary neoplasm of low malignant potential (36.4%), low-grade papillary carcinoma (90.9%), high-grade papillary carcinoma (72.7%), invasive carcinoma (14%) and epidermoid metaplasia with keratinization (45.4%). Conclusion: Our team has a vast experience on the bladder cancer animal models; however this is the first protocol in male rats. We concluded that the lesions identified are similar to those observed in Humans.
Autores principais:Faustino-Rocha, Ana Isabel
Outros Autores:Pinto, Carla; Vala, Helena; Ferreira, Rita; Oliveira, Paula Alexandra
Assunto:Bladder cancer Urinary bladder tumours N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN)
Ano:2014
País:Portugal
Tipo de documento:documento de conferência
Tipo de acesso:acesso restrito
Instituição associada:Instituto Politécnico de Viseu
Idioma:inglês
Origem:Repositório Científico do Instituto Politécnico de Viseu

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