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Prebiotic xylo-oligosaccharides as high-value co-products on an integrated biorefinery approach from lignocellulosic feedstock

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Resumo:The present work proposes the production of prebiotic xylo-oligosaccharides (XOS) as high-value co-products of the Lignocellulose Feedstock Biorefinery concept, foreseeing potential applications on food, feed and nutraceutical industries. Autohydrolysis was used to selectively solubilise the hemicellulosic fraction of several xylan-rich, widely available, agricultural, agro-industrial and forestry by-products: corn cobs, brewery’s spent grain and Eucalyptus wood chips. The soluble hemicellulose-rich and the solid cellulose- and lignin-rich fractions were separated, and the crude XOS-rich hydrolysates were further purified by gel filtration chromatography. Selected fractions of purified XOS within the desired ranges of polymerization degree were characterised and their prebiotic potential was investigated in in vitro fermentations by bifidobacteria, lactobacilli and intestinal inocula. Parameters such as bacterial growth and XOS consumption were evaluated and compared with commercially available xylo-oligosaccharides. The differences observed were considered of relevance for the formulation of symbiotic preparations and the future design of targeted, tailor-made prebiotic xylo-oligosaccharides.
Autores principais:Moura, Patrícia
Outros Autores:Carvalheiro, Florbela; Esteves, M. P.; Gírio, Francisco
Assunto:Autohydrolysis Xylo-oligosaccharide Probiotic Lignocellulosic materials Feedstock Biorefinery
Ano:2008
País:Portugal
Tipo de documento:documento de conferência
Tipo de acesso:acesso aberto
Instituição associada:Laboratório Nacional de Energia e Geologia, I.P.
Idioma:inglês
Origem:Repositório do LNEG
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author Moura, Patrícia
author2 Carvalheiro, Florbela
Esteves, M. P.
Gírio, Francisco
author2_role author
author
author
author_facet Moura, Patrícia
Carvalheiro, Florbela
Esteves, M. P.
Gírio, Francisco
author_role author
contributor_name_str_mv Repositório do LNEG
country_str PT
creators_json_str [{\"Person.name\":\"Moura, Patrícia\",\"Person.identifier.orcid\":\"0000-0001-9333-3231\"},{\"Person.name\":\"Carvalheiro, Florbela\",\"Person.identifier.orcid\":\"0000-0002-0270-4832\"},{\"Person.name\":\"Esteves, M. P.\"},{\"Person.name\":\"Gírio, Francisco\",\"Person.identifier.orcid\":\"0000-0002-5376-8430\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Repositório do LNEG
datacite.creators.creator.creatorName.fl_str_mv Moura, Patrícia
Carvalheiro, Florbela
Esteves, M. P.
Gírio, Francisco
datacite.date.Accepted.fl_str_mv 2008-04-06T00:00:00Z
datacite.date.available.fl_str_mv 2009-12-03T12:05:43Z
datacite.date.embargoed.fl_str_mv 2009-12-03T12:05:43Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.subjects.subject.fl_str_mv Autohydrolysis
Xylo-oligosaccharide
Probiotic
Lignocellulosic materials
Feedstock
Biorefinery
datacite.titles.title.fl_str_mv Prebiotic xylo-oligosaccharides as high-value co-products on an integrated biorefinery approach from lignocellulosic feedstock
dc.contributor.none.fl_str_mv Repositório do LNEG
dc.creator.none.fl_str_mv Moura, Patrícia
Carvalheiro, Florbela
Esteves, M. P.
Gírio, Francisco
dc.date.Accepted.fl_str_mv 2008-04-06T00:00:00Z
dc.date.available.fl_str_mv 2009-12-03T12:05:43Z
dc.date.embargoed.fl_str_mv 2009-12-03T12:05:43Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://hdl.handle.net/10400.9/481
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv Universidade do Minho
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.subject.none.fl_str_mv Autohydrolysis
Xylo-oligosaccharide
Probiotic
Lignocellulosic materials
Feedstock
Biorefinery
dc.title.fl_str_mv Prebiotic xylo-oligosaccharides as high-value co-products on an integrated biorefinery approach from lignocellulosic feedstock
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_c94f
description The present work proposes the production of prebiotic xylo-oligosaccharides (XOS) as high-value co-products of the Lignocellulose Feedstock Biorefinery concept, foreseeing potential applications on food, feed and nutraceutical industries. Autohydrolysis was used to selectively solubilise the hemicellulosic fraction of several xylan-rich, widely available, agricultural, agro-industrial and forestry by-products: corn cobs, brewery’s spent grain and Eucalyptus wood chips. The soluble hemicellulose-rich and the solid cellulose- and lignin-rich fractions were separated, and the crude XOS-rich hydrolysates were further purified by gel filtration chromatography. Selected fractions of purified XOS within the desired ranges of polymerization degree were characterised and their prebiotic potential was investigated in in vitro fermentations by bifidobacteria, lactobacilli and intestinal inocula. Parameters such as bacterial growth and XOS consumption were evaluated and compared with commercially available xylo-oligosaccharides. The differences observed were considered of relevance for the formulation of symbiotic preparations and the future design of targeted, tailor-made prebiotic xylo-oligosaccharides.
dirty 0
eu_rights_str_mv openAccess
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institution Laboratório Nacional de Energia e Geologia, I.P.
instname_str Laboratório Nacional de Energia e Geologia, I.P.
language eng
network_acronym_str rlneg
network_name_str Repositório do LNEG
oai_identifier_str oai:repositorio.lneg.pt:10400.9/481
organization_str_mv urn:organizationAcronym:rlneg
person_str_mv Moura, Patrícia
Moura, Patrícia
https://www.ciencia-id.pt/BA17-3D3E-5478
BA17-3D3E-5478
http://orcid.org/0000-0001-9333-3231
0000-0001-9333-3231
Carvalheiro, Florbela
Carvalheiro, Florbela
https://www.ciencia-id.pt/4016-9476-B97D
4016-9476-B97D
http://orcid.org/0000-0002-0270-4832
0000-0002-0270-4832
Esteves, M. P.
Gírio, Francisco
Gírio, Francisco
https://www.ciencia-id.pt/D51D-09A4-A2DC
D51D-09A4-A2DC
http://orcid.org/0000-0002-5376-8430
0000-0002-5376-8430
publishDate 2008
publisher.none.fl_str_mv Universidade do Minho
reponame_str Repositório do LNEG
repository_id_str urn:repositoryAcronym:rlneg
service_str_mv urn:repositoryAcronym:rlneg
spelling engUniversidade do MinhoptThe present work proposes the production of prebiotic xylo-oligosaccharides (XOS) as high-value co-products of the Lignocellulose Feedstock Biorefinery concept, foreseeing potential applications on food, feed and nutraceutical industries. Autohydrolysis was used to selectively solubilise the hemicellulosic fraction of several xylan-rich, widely available, agricultural, agro-industrial and forestry by-products: corn cobs, brewery’s spent grain and Eucalyptus wood chips. The soluble hemicellulose-rich and the solid cellulose- and lignin-rich fractions were separated, and the crude XOS-rich hydrolysates were further purified by gel filtration chromatography. Selected fractions of purified XOS within the desired ranges of polymerization degree were characterised and their prebiotic potential was investigated in in vitro fermentations by bifidobacteria, lactobacilli and intestinal inocula. Parameters such as bacterial growth and XOS consumption were evaluated and compared with commercially available xylo-oligosaccharides. The differences observed were considered of relevance for the formulation of symbiotic preparations and the future design of targeted, tailor-made prebiotic xylo-oligosaccharides.application/pdfptPrebiotic xylo-oligosaccharides as high-value co-products on an integrated biorefinery approach from lignocellulosic feedstockPersonalMoura, PatríciaDSpacehttp://dspace.org/items/b0ec9a1e-8b38-48e2-a76b-a09e13975dcfDSpacehttp://dspace.org/items/b0ec9a1e-8b38-48e2-a76b-a09e13975dcfMouraPatríciaCiência IDhttps://www.ciencia-id.ptBA17-3D3E-5478ORCIDhttp://orcid.org0000-0001-9333-3231Researcher IDhttps://www.researcherid.comA-8330-2015PersonalCarvalheiro, FlorbelaDSpacehttp://dspace.org/items/cdbcb5eb-94de-49eb-920b-089980c34cbfDSpacehttp://dspace.org/items/cdbcb5eb-94de-49eb-920b-089980c34cbfCarvalheiroFlorbelaCiência IDhttps://www.ciencia-id.pt4016-9476-B97DORCIDhttp://orcid.org0000-0002-0270-4832Researcher IDhttps://www.researcherid.comL-1860-2014Scopus Author IDhttps://www.scopus.com6603247136Esteves, M. P.PersonalGírio, FranciscoDSpacehttp://dspace.org/items/781a0b63-529e-4c34-8dcc-fd27e785e762DSpacehttp://dspace.org/items/781a0b63-529e-4c34-8dcc-fd27e785e762FERREIRA GIRIOFRANCISCO MANUELCiência IDhttps://www.ciencia-id.ptD51D-09A4-A2DCORCIDhttp://orcid.org0000-0002-5376-8430HostingInstitutionOrganizationalRepositório do LNEGe-mailmailto:repositorio@lneg.ptrepositorio@lneg.pt2009-12-03T12:05:43Z2008-04-062008-04-06T00:00:00ZHandlehttp://hdl.handle.net/10400.9/481http://purl.org/coar/access_right/c_abf2open accessAutohydrolysisXylo-oligosaccharideProbioticLignocellulosic materialsFeedstockBiorefinery110861 bytesother research producthttp://purl.org/coar/resource_type/c_c94fconference objecthttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.lneg.pt/bitstreams/5ad95f82-35d0-4439-b2ac-3bc7e8ab14ec/downloadBioenergy: Challenges and Opportunities, International Conference and Exhibition on BioenergyUniversidade do Minho, Guimarães
spellingShingle Prebiotic xylo-oligosaccharides as high-value co-products on an integrated biorefinery approach from lignocellulosic feedstock
Moura, Patrícia
Autohydrolysis
Xylo-oligosaccharide
Probiotic
Lignocellulosic materials
Feedstock
Biorefinery
subject.fl_str_mv Autohydrolysis
Xylo-oligosaccharide
Probiotic
Lignocellulosic materials
Feedstock
Biorefinery
title Prebiotic xylo-oligosaccharides as high-value co-products on an integrated biorefinery approach from lignocellulosic feedstock
title_full Prebiotic xylo-oligosaccharides as high-value co-products on an integrated biorefinery approach from lignocellulosic feedstock
title_fullStr Prebiotic xylo-oligosaccharides as high-value co-products on an integrated biorefinery approach from lignocellulosic feedstock
title_full_unstemmed Prebiotic xylo-oligosaccharides as high-value co-products on an integrated biorefinery approach from lignocellulosic feedstock
title_short Prebiotic xylo-oligosaccharides as high-value co-products on an integrated biorefinery approach from lignocellulosic feedstock
title_sort Prebiotic xylo-oligosaccharides as high-value co-products on an integrated biorefinery approach from lignocellulosic feedstock
topic Autohydrolysis
Xylo-oligosaccharide
Probiotic
Lignocellulosic materials
Feedstock
Biorefinery
topic_facet Autohydrolysis
Xylo-oligosaccharide
Probiotic
Lignocellulosic materials
Feedstock
Biorefinery
url http://hdl.handle.net/10400.9/481
visible 1