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The key role of sulfation and branching on fucoidan antitumor activity

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Resumo:There is an urgent need for antitumor bioactive agents with minimal or no side effects over normal adjacent cells. Fucoidan is a marine-origin polymer with known antitumor activity. However, there are still some concerns about its application due to the inconsistent experimental results, specifically its toxicity over normal cells and the mechanism behind its action. Herein, three fucoidan extracts (FEs) have been tested over normal and breast cancer cell lines. From cytotoxicity results, only one of the extracts shows selective antitumor behavior (at 0.2 mg mLâ 1), despite similarities in sulfation degree and carbohydrates composition. Although the three FEs present different molecular weights, depolymerization of selected samples discarded Mw as the key factor in the antitumor activity. Significant differences in sulfates position and branching are observed, presenting FE 2 the higher branching degree. Based on all these experimental data, it is believed that these last two properties are the ones that influence the cytotoxic effects of fucoidan extracts.
Autores principais:Oliveira, Catarina
Outros Autores:Ferreira, Andreia S.; Novoa-Carballal, R.; Nunes, Cláudia; Pashkuleva, I.; Neves, N. M.; Coimbra, Manuel A.; Reis, R. L.; Martins, Albino; Silva, Tiago José Quinteiros Lopes Henriques
Assunto:Antitumor activity Fucoidan Polymer branching Structure–activity relationship Sulfation
Ano:2017
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho
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author Oliveira, Catarina
author2 Ferreira, Andreia S.
Novoa-Carballal, R.
Nunes, Cláudia
Pashkuleva, I.
Neves, N. M.
Coimbra, Manuel A.
Reis, R. L.
Martins, Albino
Silva, Tiago José Quinteiros Lopes Henriques
author2_role author
author
author
author
author
author
author
author
author
author_facet Oliveira, Catarina
Ferreira, Andreia S.
Novoa-Carballal, R.
Nunes, Cláudia
Pashkuleva, I.
Neves, N. M.
Coimbra, Manuel A.
Reis, R. L.
Martins, Albino
Silva, Tiago José Quinteiros Lopes Henriques
author_role author
contributor_name_str_mv Universidade do Minho
country_str PT
creators_json_txt [{\"Person.name\":\"Oliveira, Catarina\"},{\"Person.name\":\"Ferreira, Andreia S.\"},{\"Person.name\":\"Novoa-Carballal, R.\"},{\"Person.name\":\"Nunes, Cláudia\"},{\"Person.name\":\"Pashkuleva, I.\"},{\"Person.name\":\"Neves, N. M.\"},{\"Person.name\":\"Coimbra, Manuel A.\"},{\"Person.name\":\"Reis, R. L.\"},{\"Person.name\":\"Martins, Albino\"},{\"Person.name\":\"Silva, Tiago José Quinteiros Lopes Henriques\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Universidade do Minho
datacite.creators.creator.creatorName.fl_str_mv Oliveira, Catarina
Ferreira, Andreia S.
Novoa-Carballal, R.
Nunes, Cláudia
Pashkuleva, I.
Neves, N. M.
Coimbra, Manuel A.
Reis, R. L.
Martins, Albino
Silva, Tiago José Quinteiros Lopes Henriques
datacite.date.Accepted.fl_str_mv 2017-01-01T00:00:00Z
datacite.date.available.fl_str_mv 2017-01-18T15:41:08Z
datacite.date.embargoed.fl_str_mv 2017-01-18T15:41:08Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.subjects.subject.fl_str_mv Antitumor activity
Fucoidan
Polymer branching
Structure–activity relationship
Sulfation
datacite.titles.title.fl_str_mv The key role of sulfation and branching on fucoidan antitumor activity
dc.contributor.none.fl_str_mv Universidade do Minho
dc.creator.none.fl_str_mv Oliveira, Catarina
Ferreira, Andreia S.
Novoa-Carballal, R.
Nunes, Cláudia
Pashkuleva, I.
Neves, N. M.
Coimbra, Manuel A.
Reis, R. L.
Martins, Albino
Silva, Tiago José Quinteiros Lopes Henriques
dc.date.Accepted.fl_str_mv 2017-01-01T00:00:00Z
dc.date.available.fl_str_mv 2017-01-18T15:41:08Z
dc.date.embargoed.fl_str_mv 2017-01-18T15:41:08Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv https://hdl.handle.net/1822/44382
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv John Wiley and Sons
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.subject.none.fl_str_mv Antitumor activity
Fucoidan
Polymer branching
Structure–activity relationship
Sulfation
dc.title.fl_str_mv The key role of sulfation and branching on fucoidan antitumor activity
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
description There is an urgent need for antitumor bioactive agents with minimal or no side effects over normal adjacent cells. Fucoidan is a marine-origin polymer with known antitumor activity. However, there are still some concerns about its application due to the inconsistent experimental results, specifically its toxicity over normal cells and the mechanism behind its action. Herein, three fucoidan extracts (FEs) have been tested over normal and breast cancer cell lines. From cytotoxicity results, only one of the extracts shows selective antitumor behavior (at 0.2 mg mLâ 1), despite similarities in sulfation degree and carbohydrates composition. Although the three FEs present different molecular weights, depolymerization of selected samples discarded Mw as the key factor in the antitumor activity. Significant differences in sulfates position and branching are observed, presenting FE 2 the higher branching degree. Based on all these experimental data, it is believed that these last two properties are the ones that influence the cytotoxic effects of fucoidan extracts.
dirty 0
eu_rights_str_mv openAccess
format article
fulltext.url.fl_str_mv https://prod-dspace.uminho.pt/bitstreams/4dc138b6-29bd-48b9-a253-31b38035acb4/download
id rum_051a597bd18caebdc6f330c00b869394
identifier.url.fl_str_mv https://hdl.handle.net/1822/44382
instacron_str repositorium
institution Universidade do Minho
instname_str Universidade do Minho
language eng
network_acronym_str rum
network_name_str RepositóriUM - Universidade do Minho
oai_identifier_str oai:repositorium.uminho.pt:1822/44382
organization_str_mv urn:organizationAcronym:repositorium
person_str_mv Oliveira, Catarina
Ferreira, Andreia S.
Novoa-Carballal, R.
Nunes, Cláudia
Pashkuleva, I.
Neves, N. M.
Coimbra, Manuel A.
Reis, R. L.
Martins, Albino
Silva, Tiago José Quinteiros Lopes Henriques
publishDate 2017
publisher.none.fl_str_mv John Wiley and Sons
reponame_str RepositóriUM - Universidade do Minho
repository_id_str urn:repositoryAcronym:rum
service_str_mv urn:repositoryAcronym:rum
spelling engJohn Wiley and SonsporThere is an urgent need for antitumor bioactive agents with minimal or no side effects over normal adjacent cells. Fucoidan is a marine-origin polymer with known antitumor activity. However, there are still some concerns about its application due to the inconsistent experimental results, specifically its toxicity over normal cells and the mechanism behind its action. Herein, three fucoidan extracts (FEs) have been tested over normal and breast cancer cell lines. From cytotoxicity results, only one of the extracts shows selective antitumor behavior (at 0.2 mg mLâ 1), despite similarities in sulfation degree and carbohydrates composition. Although the three FEs present different molecular weights, depolymerization of selected samples discarded Mw as the key factor in the antitumor activity. Significant differences in sulfates position and branching are observed, presenting FE 2 the higher branching degree. Based on all these experimental data, it is believed that these last two properties are the ones that influence the cytotoxic effects of fucoidan extracts.application/pdfporThe key role of sulfation and branching on fucoidan antitumor activityOliveira, CatarinaFerreira, Andreia S.Novoa-Carballal, R.Nunes, CláudiaPashkuleva, I.Neves, N. M.Coimbra, Manuel A.Reis, R. L.Martins, AlbinoSilva, Tiago José Quinteiros Lopes HenriquesHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf1616-5195DOIIsPartOf10.1002/mabi.2016003402017-01-18T15:41:08Z20172017-012017-01-11T14:28:50Z2017-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/44382http://purl.org/coar/access_right/c_abf2open accessAntitumor activityFucoidanPolymer branchingStructure–activity relationshipSulfation2537622 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/4dc138b6-29bd-48b9-a253-31b38035acb4/download
spellingShingle The key role of sulfation and branching on fucoidan antitumor activity
Oliveira, Catarina
Antitumor activity
Fucoidan
Polymer branching
Structure–activity relationship
Sulfation
status SINGLETON
subject.fl_str_mv Antitumor activity
Fucoidan
Polymer branching
Structure–activity relationship
Sulfation
title The key role of sulfation and branching on fucoidan antitumor activity
title_full The key role of sulfation and branching on fucoidan antitumor activity
title_fullStr The key role of sulfation and branching on fucoidan antitumor activity
title_full_unstemmed The key role of sulfation and branching on fucoidan antitumor activity
title_short The key role of sulfation and branching on fucoidan antitumor activity
title_sort The key role of sulfation and branching on fucoidan antitumor activity
topic Antitumor activity
Fucoidan
Polymer branching
Structure–activity relationship
Sulfation
topic_facet Antitumor activity
Fucoidan
Polymer branching
Structure–activity relationship
Sulfation
url https://hdl.handle.net/1822/44382
visible 1