Publicação
Neovascularization induced by the hyaluronic acid-based spongy-like hydrogels degradation products
| Resumo: | Neovascularization has been a major challenge in many tissue regeneration strategies. Hyaluronic acid (HA) of 3â 25 disaccharides is known to be angiogenic due to its interaction with endothelial cell receptors. This effect has been explored with HA-based structures but a transitory response is observed due to HA burst biodegradation. Herein we developed gellan gum (GG)-HA spongy-like hydrogels from semi-interpenetrating network hydrogels with different HA amounts. Enzymatic degradation was more evident in the GG-HA with high HA amount due to their lower mechanical stability, also resulting from the degradation itself, which facilitated the access of the enzyme to the HA in the bulk. GG-HA spongy-like hydrogels hyaluronidase-mediated degradation lead to the release of HA oligosaccharides of different amounts and sizes in a HA content-dependent manner which promoted in vitro proliferation of human umbilical cord vein endothelial cells (HUVECs) but not their migration. Although no effect was observed in human dermal microvascular endothelial cells (hDMECs) in vitro, the implantation of GG-HA spongy-like hydrogels in an ischemic hind limb mice model promoted neovascularization in a material-dependent manner, consistent with the in vitro degradation profile. Overall, GG-HA spongy-like hydrogels with a sustained release of HA oligomers are valuable options to improve tissue vascularization, a critical issue in several applications in the tissue engineering and regenerative medicine field. |
|---|---|
| Autores principais: | Silva, Lucília Pereira |
| Outros Autores: | Pirraco, Rogério P.; Santos, T. C.; Novoa-Carballal, R.; Cerqueira, Mariana Teixeira; Reis, R. L.; Correlo, V. M.; Marques, A. P. |
| Assunto: | Angiogenesis Biodegradation gellan gum spongy-like hydrogels Hyaluronic acid Ischemia Neovascularization |
| Ano: | 2016 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866878108422897664 |
|---|---|
| author | Silva, Lucília Pereira |
| author2 | Pirraco, Rogério P. Santos, T. C. Novoa-Carballal, R. Cerqueira, Mariana Teixeira Reis, R. L. Correlo, V. M. Marques, A. P. |
| author2_role | author author author author author author author |
| author_facet | Silva, Lucília Pereira Pirraco, Rogério P. Santos, T. C. Novoa-Carballal, R. Cerqueira, Mariana Teixeira Reis, R. L. Correlo, V. M. Marques, A. P. |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Silva, Lucília Pereira\"},{\"Person.name\":\"Pirraco, Rogério P.\"},{\"Person.name\":\"Santos, T. C.\"},{\"Person.name\":\"Novoa-Carballal, R.\"},{\"Person.name\":\"Cerqueira, Mariana Teixeira\"},{\"Person.name\":\"Reis, R. L.\"},{\"Person.name\":\"Correlo, V. M.\"},{\"Person.name\":\"Marques, A. P.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Silva, Lucília Pereira Pirraco, Rogério P. Santos, T. C. Novoa-Carballal, R. Cerqueira, Mariana Teixeira Reis, R. L. Correlo, V. M. Marques, A. P. |
| datacite.date.Accepted.fl_str_mv | 2016-11-01T00:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Angiogenesis Biodegradation gellan gum spongy-like hydrogels Hyaluronic acid Ischemia Neovascularization |
| datacite.titles.title.fl_str_mv | Neovascularization induced by the hyaluronic acid-based spongy-like hydrogels degradation products |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Silva, Lucília Pereira Pirraco, Rogério P. Santos, T. C. Novoa-Carballal, R. Cerqueira, Mariana Teixeira Reis, R. L. Correlo, V. M. Marques, A. P. |
| dc.date.Accepted.fl_str_mv | 2016-11-01T00:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/43834 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | ACS Publications |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Angiogenesis Biodegradation gellan gum spongy-like hydrogels Hyaluronic acid Ischemia Neovascularization |
| dc.title.fl_str_mv | Neovascularization induced by the hyaluronic acid-based spongy-like hydrogels degradation products |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Neovascularization has been a major challenge in many tissue regeneration strategies. Hyaluronic acid (HA) of 3â 25 disaccharides is known to be angiogenic due to its interaction with endothelial cell receptors. This effect has been explored with HA-based structures but a transitory response is observed due to HA burst biodegradation. Herein we developed gellan gum (GG)-HA spongy-like hydrogels from semi-interpenetrating network hydrogels with different HA amounts. Enzymatic degradation was more evident in the GG-HA with high HA amount due to their lower mechanical stability, also resulting from the degradation itself, which facilitated the access of the enzyme to the HA in the bulk. GG-HA spongy-like hydrogels hyaluronidase-mediated degradation lead to the release of HA oligosaccharides of different amounts and sizes in a HA content-dependent manner which promoted in vitro proliferation of human umbilical cord vein endothelial cells (HUVECs) but not their migration. Although no effect was observed in human dermal microvascular endothelial cells (hDMECs) in vitro, the implantation of GG-HA spongy-like hydrogels in an ischemic hind limb mice model promoted neovascularization in a material-dependent manner, consistent with the in vitro degradation profile. Overall, GG-HA spongy-like hydrogels with a sustained release of HA oligomers are valuable options to improve tissue vascularization, a critical issue in several applications in the tissue engineering and regenerative medicine field. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/0240732e-6662-4407-9b4a-eea5ae4e2d27/download |
| id | rum_1131f622eb2e0fee74af7e2e8ea8283f |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/43834 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/43834 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Silva, Lucília Pereira Pirraco, Rogério P. Santos, T. C. Novoa-Carballal, R. Cerqueira, Mariana Teixeira Reis, R. L. Correlo, V. M. Marques, A. P. |
| publishDate | 2016 |
| publisher.none.fl_str_mv | ACS Publications |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engACS PublicationsporNeovascularization has been a major challenge in many tissue regeneration strategies. Hyaluronic acid (HA) of 3â 25 disaccharides is known to be angiogenic due to its interaction with endothelial cell receptors. This effect has been explored with HA-based structures but a transitory response is observed due to HA burst biodegradation. Herein we developed gellan gum (GG)-HA spongy-like hydrogels from semi-interpenetrating network hydrogels with different HA amounts. Enzymatic degradation was more evident in the GG-HA with high HA amount due to their lower mechanical stability, also resulting from the degradation itself, which facilitated the access of the enzyme to the HA in the bulk. GG-HA spongy-like hydrogels hyaluronidase-mediated degradation lead to the release of HA oligosaccharides of different amounts and sizes in a HA content-dependent manner which promoted in vitro proliferation of human umbilical cord vein endothelial cells (HUVECs) but not their migration. Although no effect was observed in human dermal microvascular endothelial cells (hDMECs) in vitro, the implantation of GG-HA spongy-like hydrogels in an ischemic hind limb mice model promoted neovascularization in a material-dependent manner, consistent with the in vitro degradation profile. Overall, GG-HA spongy-like hydrogels with a sustained release of HA oligomers are valuable options to improve tissue vascularization, a critical issue in several applications in the tissue engineering and regenerative medicine field.application/pdfporNeovascularization induced by the hyaluronic acid-based spongy-like hydrogels degradation productsSilva, Lucília PereiraPirraco, Rogério P.Santos, T. C.Novoa-Carballal, R.Cerqueira, Mariana TeixeiraReis, R. L.Correlo, V. M.Marques, A. P.HostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf1944-8252DOIIsPartOf10.1021/acsami.6b116842016-112016-122016-12-16T10:04:36Z2016-11-01T00:00:00ZHandlehttps://hdl.handle.net/1822/43834http://purl.org/coar/access_right/c_16ecrestricted accessAngiogenesisBiodegradationgellan gum spongy-like hydrogelsHyaluronic acidIschemiaNeovascularization2275126 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/0240732e-6662-4407-9b4a-eea5ae4e2d27/download |
| spellingShingle | Neovascularization induced by the hyaluronic acid-based spongy-like hydrogels degradation products Silva, Lucília Pereira Angiogenesis Biodegradation gellan gum spongy-like hydrogels Hyaluronic acid Ischemia Neovascularization |
| status | SINGLETON |
| subject.fl_str_mv | Angiogenesis Biodegradation gellan gum spongy-like hydrogels Hyaluronic acid Ischemia Neovascularization |
| title | Neovascularization induced by the hyaluronic acid-based spongy-like hydrogels degradation products |
| title_full | Neovascularization induced by the hyaluronic acid-based spongy-like hydrogels degradation products |
| title_fullStr | Neovascularization induced by the hyaluronic acid-based spongy-like hydrogels degradation products |
| title_full_unstemmed | Neovascularization induced by the hyaluronic acid-based spongy-like hydrogels degradation products |
| title_short | Neovascularization induced by the hyaluronic acid-based spongy-like hydrogels degradation products |
| title_sort | Neovascularization induced by the hyaluronic acid-based spongy-like hydrogels degradation products |
| topic | Angiogenesis Biodegradation gellan gum spongy-like hydrogels Hyaluronic acid Ischemia Neovascularization |
| topic_facet | Angiogenesis Biodegradation gellan gum spongy-like hydrogels Hyaluronic acid Ischemia Neovascularization |
| url | https://hdl.handle.net/1822/43834 |
| visible | 1 |