Publicação
Proteomic responses to nanoparticulate and ionic silver in aquatic fungi
| Resumo: | Enhanced use of silver nanoparticles (AgNPs) has inevitably resulted in their release into aquatic environments raising concern about the risk to aquatic biota and related ecological functions. Functional proteomics is an emerging technology that provides high-throughput analyses augmenting measurements of direct and highly sensitive responses at the cellular and sub-cellular levels. The impacts of AgNPs and its ionic precursor (Ag+ in AgNO3) at low exposure concentrations (close to environmental realism) on a fungal strain isolated from a non-polluted stream were assessed based on the variations in the overall proteome as well as in the activity of selected antioxidant enzymes. A total of 352 proteins were identified, but only 151 proteins were responsive (significantly up- or down-regulated relative to control) of which 65% presented matching alterations. Out of these 151 proteins, 62% increased abundance under stress induced by AgNPs and 56% under stress induced by Ag+. Exposure to both forms of silver induced proteins related to stress response, in particular, antioxidant enzymes. The antioxidant enzymatic responses were consistent with the proteomic responses, suggesting that the ability to initiate an efficient antioxidant response is essential for the fungus to cope with Ag-induced toxicity. Moreover, several proteins involved in the metabolism of carbohydrates, amino acids and lipids were altered. This evidence may reflect the need of generating energy to support the cellular defense mechanisms. Some of the significantly altered proteins were associated with the correct folding of nascent and stress accumulated misfolded proteins or degradation of transiently denatured and unfolded proteins preventing their aggregation. Others were related to the regulation of translation suggesting a compromised protein synthesis system. Overall, the functional proteomic approach can be useful to expand the knowledge on silver-induced stress responses in aquatic fungi |
|---|---|
| Autores principais: | Barros, Diana Cláudia Martins Costa |
| Outros Autores: | Pradhan, Arunava; Pascoal, Cláudia; Cássio, Fernanda |
| Assunto: | Silver nanoparticles Stress response Functional proteomics Antioxidant enzymes Aquatic fungi Ciências Naturais::Ciências Biológicas |
| Ano: | 2016 |
| País: | Portugal |
| Tipo de documento: | póster em conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867439267870932992 |
|---|---|
| author | Barros, Diana Cláudia Martins Costa |
| author2 | Pradhan, Arunava Pascoal, Cláudia Cássio, Fernanda |
| author2_role | author author author |
| author_facet | Barros, Diana Cláudia Martins Costa Pradhan, Arunava Pascoal, Cláudia Cássio, Fernanda |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Barros, Diana Cláudia Martins Costa\"},{\"Person.name\":\"Pradhan, Arunava\"},{\"Person.name\":\"Pascoal, Cláudia\"},{\"Person.name\":\"Cássio, Fernanda\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Barros, Diana Cláudia Martins Costa Pradhan, Arunava Pascoal, Cláudia Cássio, Fernanda |
| datacite.date.Accepted.fl_str_mv | 2016-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2021-05-28T16:35:42Z |
| datacite.date.embargoed.fl_str_mv | 2021-05-28T16:35:42Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Silver nanoparticles Stress response Functional proteomics Antioxidant enzymes Aquatic fungi Ciências Naturais::Ciências Biológicas |
| datacite.titles.title.fl_str_mv | Proteomic responses to nanoparticulate and ionic silver in aquatic fungi |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Barros, Diana Cláudia Martins Costa Pradhan, Arunava Pascoal, Cláudia Cássio, Fernanda |
| dc.date.Accepted.fl_str_mv | 2016-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2021-05-28T16:35:42Z |
| dc.date.embargoed.fl_str_mv | 2021-05-28T16:35:42Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/73063 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Silver nanoparticles Stress response Functional proteomics Antioxidant enzymes Aquatic fungi Ciências Naturais::Ciências Biológicas |
| dc.title.fl_str_mv | Proteomic responses to nanoparticulate and ionic silver in aquatic fungi |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6670 |
| description | Enhanced use of silver nanoparticles (AgNPs) has inevitably resulted in their release into aquatic environments raising concern about the risk to aquatic biota and related ecological functions. Functional proteomics is an emerging technology that provides high-throughput analyses augmenting measurements of direct and highly sensitive responses at the cellular and sub-cellular levels. The impacts of AgNPs and its ionic precursor (Ag+ in AgNO3) at low exposure concentrations (close to environmental realism) on a fungal strain isolated from a non-polluted stream were assessed based on the variations in the overall proteome as well as in the activity of selected antioxidant enzymes. A total of 352 proteins were identified, but only 151 proteins were responsive (significantly up- or down-regulated relative to control) of which 65% presented matching alterations. Out of these 151 proteins, 62% increased abundance under stress induced by AgNPs and 56% under stress induced by Ag+. Exposure to both forms of silver induced proteins related to stress response, in particular, antioxidant enzymes. The antioxidant enzymatic responses were consistent with the proteomic responses, suggesting that the ability to initiate an efficient antioxidant response is essential for the fungus to cope with Ag-induced toxicity. Moreover, several proteins involved in the metabolism of carbohydrates, amino acids and lipids were altered. This evidence may reflect the need of generating energy to support the cellular defense mechanisms. Some of the significantly altered proteins were associated with the correct folding of nascent and stress accumulated misfolded proteins or degradation of transiently denatured and unfolded proteins preventing their aggregation. Others were related to the regulation of translation suggesting a compromised protein synthesis system. Overall, the functional proteomic approach can be useful to expand the knowledge on silver-induced stress responses in aquatic fungi |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | conferencePoster |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/645cdff7-4281-4644-9c9c-7ac3c7170177/download |
| id | rum_1f87fc9ab45b7403d80c19a269253e90 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/73063 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/73063 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Barros, Diana Cláudia Martins Costa Pradhan, Arunava Pascoal, Cláudia Cássio, Fernanda |
| publishDate | 2016 |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engporEnhanced use of silver nanoparticles (AgNPs) has inevitably resulted in their release into aquatic environments raising concern about the risk to aquatic biota and related ecological functions. Functional proteomics is an emerging technology that provides high-throughput analyses augmenting measurements of direct and highly sensitive responses at the cellular and sub-cellular levels. The impacts of AgNPs and its ionic precursor (Ag+ in AgNO3) at low exposure concentrations (close to environmental realism) on a fungal strain isolated from a non-polluted stream were assessed based on the variations in the overall proteome as well as in the activity of selected antioxidant enzymes. A total of 352 proteins were identified, but only 151 proteins were responsive (significantly up- or down-regulated relative to control) of which 65% presented matching alterations. Out of these 151 proteins, 62% increased abundance under stress induced by AgNPs and 56% under stress induced by Ag+. Exposure to both forms of silver induced proteins related to stress response, in particular, antioxidant enzymes. The antioxidant enzymatic responses were consistent with the proteomic responses, suggesting that the ability to initiate an efficient antioxidant response is essential for the fungus to cope with Ag-induced toxicity. Moreover, several proteins involved in the metabolism of carbohydrates, amino acids and lipids were altered. This evidence may reflect the need of generating energy to support the cellular defense mechanisms. Some of the significantly altered proteins were associated with the correct folding of nascent and stress accumulated misfolded proteins or degradation of transiently denatured and unfolded proteins preventing their aggregation. Others were related to the regulation of translation suggesting a compromised protein synthesis system. Overall, the functional proteomic approach can be useful to expand the knowledge on silver-induced stress responses in aquatic fungiapplication/pdfporProteomic responses to nanoparticulate and ionic silver in aquatic fungiBarros, Diana Cláudia Martins CostaPradhan, ArunavaPascoal, CláudiaCássio, FernandaHostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.pt2021-05-28T16:35:42Z20162016-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/73063http://purl.org/coar/access_right/c_abf2open accessSilver nanoparticlesStress responseFunctional proteomicsAntioxidant enzymesAquatic fungihttp://www.oecd.org/science/inno/38235147.pdfFields of Science and Technology (FOS)Ciências Naturais::Ciências Biológicas32676 bytesother research producthttp://purl.org/coar/resource_type/c_6670conference posterhttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/645cdff7-4281-4644-9c9c-7ac3c7170177/download |
| spellingShingle | Proteomic responses to nanoparticulate and ionic silver in aquatic fungi Barros, Diana Cláudia Martins Costa Silver nanoparticles Stress response Functional proteomics Antioxidant enzymes Aquatic fungi Ciências Naturais::Ciências Biológicas |
| status | SINGLETON |
| subject.fl_str_mv | Silver nanoparticles Stress response Functional proteomics Antioxidant enzymes Aquatic fungi |
| subject.other.fl_str_mv | Ciências Naturais::Ciências Biológicas |
| title | Proteomic responses to nanoparticulate and ionic silver in aquatic fungi |
| title_full | Proteomic responses to nanoparticulate and ionic silver in aquatic fungi |
| title_fullStr | Proteomic responses to nanoparticulate and ionic silver in aquatic fungi |
| title_full_unstemmed | Proteomic responses to nanoparticulate and ionic silver in aquatic fungi |
| title_short | Proteomic responses to nanoparticulate and ionic silver in aquatic fungi |
| title_sort | Proteomic responses to nanoparticulate and ionic silver in aquatic fungi |
| topic | Silver nanoparticles Stress response Functional proteomics Antioxidant enzymes Aquatic fungi Ciências Naturais::Ciências Biológicas |
| topic_facet | Silver nanoparticles Stress response Functional proteomics Antioxidant enzymes Aquatic fungi Ciências Naturais::Ciências Biológicas |
| url | https://hdl.handle.net/1822/73063 |
| visible | 1 |