Publicação
Expressing and functional analysis of mammalian apoptotic regulators in yeast
| Resumo: | The ease by which yeast can be manipulated in conjunction with their similarities to cells of more complex metazoans makes many yeast species, particularly Saccharomyces cerevisae, very attractive models for the study of conserved evolutionary processes that occur in eukaryotes. The ability to functionally express heterologous genes in these cells has allowed the development of countless new and elegant approaches leading to detailed structure-function analysis of numerous mammalian genes. Of these, the most informative have been the studies involving the analysis of regulators that have no direct or obvious sequence orthologue in yeast, including members of the Bcl-2 family of proteins, caspases and tumour suppressors. Here we review the field and provide evidence that these studies have served to further understand mammalian apoptosis. |
|---|---|
| Autores principais: | Greenwood, M. T. |
| Outros Autores: | Ludovico, Paula |
| Assunto: | Animals Apoptosis Humans Mammals Models, Biological Proto-Oncogene Proteins c-bcl-2 Saccharomyces cerevisiae Bcl-2 family members caspases tumour suppressors Bax-mediated cell death |
| Ano: | 2010 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866877605136826368 |
|---|---|
| author | Greenwood, M. T. |
| author2 | Ludovico, Paula |
| author2_role | author |
| author_facet | Greenwood, M. T. Ludovico, Paula |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Greenwood, M. T.\"},{\"Person.name\":\"Ludovico, Paula\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Greenwood, M. T. Ludovico, Paula |
| datacite.date.Accepted.fl_str_mv | 2010-05-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2020-10-16T14:28:41Z |
| datacite.date.embargoed.fl_str_mv | 2020-10-16T14:28:41Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Animals Apoptosis Humans Mammals Models, Biological Proto-Oncogene Proteins c-bcl-2 Saccharomyces cerevisiae Bcl-2 family members caspases tumour suppressors Bax-mediated cell death |
| datacite.titles.title.fl_str_mv | Expressing and functional analysis of mammalian apoptotic regulators in yeast |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Greenwood, M. T. Ludovico, Paula |
| dc.date.Accepted.fl_str_mv | 2010-05-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2020-10-16T14:28:41Z |
| dc.date.embargoed.fl_str_mv | 2020-10-16T14:28:41Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/67540 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Springer Nature |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Animals Apoptosis Humans Mammals Models, Biological Proto-Oncogene Proteins c-bcl-2 Saccharomyces cerevisiae Bcl-2 family members caspases tumour suppressors Bax-mediated cell death |
| dc.title.fl_str_mv | Expressing and functional analysis of mammalian apoptotic regulators in yeast |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The ease by which yeast can be manipulated in conjunction with their similarities to cells of more complex metazoans makes many yeast species, particularly Saccharomyces cerevisae, very attractive models for the study of conserved evolutionary processes that occur in eukaryotes. The ability to functionally express heterologous genes in these cells has allowed the development of countless new and elegant approaches leading to detailed structure-function analysis of numerous mammalian genes. Of these, the most informative have been the studies involving the analysis of regulators that have no direct or obvious sequence orthologue in yeast, including members of the Bcl-2 family of proteins, caspases and tumour suppressors. Here we review the field and provide evidence that these studies have served to further understand mammalian apoptosis. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/82548b87-fcb6-441c-a4d0-c8f4f403842c/download |
| id | rum_244c8d5bdf7ba0ea08bdc7b1bbdf3636 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/67540 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/67540 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Greenwood, M. T. Ludovico, Paula |
| publishDate | 2010 |
| publisher.none.fl_str_mv | Springer Nature |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engSpringer NatureporThe ease by which yeast can be manipulated in conjunction with their similarities to cells of more complex metazoans makes many yeast species, particularly Saccharomyces cerevisae, very attractive models for the study of conserved evolutionary processes that occur in eukaryotes. The ability to functionally express heterologous genes in these cells has allowed the development of countless new and elegant approaches leading to detailed structure-function analysis of numerous mammalian genes. Of these, the most informative have been the studies involving the analysis of regulators that have no direct or obvious sequence orthologue in yeast, including members of the Bcl-2 family of proteins, caspases and tumour suppressors. Here we review the field and provide evidence that these studies have served to further understand mammalian apoptosis.application/pdfporExpressing and functional analysis of mammalian apoptotic regulators in yeastGreenwood, M. T.Ludovico, PaulaHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf1350-9047DOIIsPartOf10.1038/cdd.2009.1772020-10-16T14:28:41Z2010-052010-05-01T00:00:00ZHandlehttps://hdl.handle.net/1822/67540http://purl.org/coar/access_right/c_abf2open accessAnimalsApoptosisHumansMammalsModels, BiologicalProto-Oncogene Proteins c-bcl-2Saccharomyces cerevisiaeBcl-2 family memberscaspasestumour suppressorsBax-mediated cell death571980 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/82548b87-fcb6-441c-a4d0-c8f4f403842c/download |
| spellingShingle | Expressing and functional analysis of mammalian apoptotic regulators in yeast Greenwood, M. T. Animals Apoptosis Humans Mammals Models, Biological Proto-Oncogene Proteins c-bcl-2 Saccharomyces cerevisiae Bcl-2 family members caspases tumour suppressors Bax-mediated cell death |
| status | SINGLETON |
| subject.fl_str_mv | Animals Apoptosis Humans Mammals Models, Biological Proto-Oncogene Proteins c-bcl-2 Saccharomyces cerevisiae Bcl-2 family members caspases tumour suppressors Bax-mediated cell death |
| title | Expressing and functional analysis of mammalian apoptotic regulators in yeast |
| title_full | Expressing and functional analysis of mammalian apoptotic regulators in yeast |
| title_fullStr | Expressing and functional analysis of mammalian apoptotic regulators in yeast |
| title_full_unstemmed | Expressing and functional analysis of mammalian apoptotic regulators in yeast |
| title_short | Expressing and functional analysis of mammalian apoptotic regulators in yeast |
| title_sort | Expressing and functional analysis of mammalian apoptotic regulators in yeast |
| topic | Animals Apoptosis Humans Mammals Models, Biological Proto-Oncogene Proteins c-bcl-2 Saccharomyces cerevisiae Bcl-2 family members caspases tumour suppressors Bax-mediated cell death |
| topic_facet | Animals Apoptosis Humans Mammals Models, Biological Proto-Oncogene Proteins c-bcl-2 Saccharomyces cerevisiae Bcl-2 family members caspases tumour suppressors Bax-mediated cell death |
| url | https://hdl.handle.net/1822/67540 |
| visible | 1 |