Publicação
High-throughput fabrication of cell-laden 3D biomaterial gradients
| Resumo: | High-throughput strategies for optimizing biomaterials to direct cellular behaviour are a fundamental need for propelling tissue engineering and regenerative medicine. In 2D, biomaterialâ s gradients have proven to be powerful platforms for simultaneously screening several surface conditions. However, their translation to 3D is yet limited to 1) exploiting light-based crosslinking and 2) non-sequential, single-gradient production. We built a microfluidic platform that allows distinct hydrogel precursors, as fluids, to be gradually mixed and crosslinked into 3D gradient fibres. Herein, we report how this system can be used for the sequential fabrication of independent cell-laden libraries with gradients of polymer concentration, non-adhesive/adhesive materials and both ionic and light crosslinking mechanisms. Automated image analysis of hundreds of single-cell events as a function of position yielded trends and pinpointed best-fit conditions based on cell shape, adhesion, proliferation and triggering of stromal/stem cell differentiation. We deliver a simple, versatile, and complete approach towards fully high-throughput 3D gradient fabrication for cell/material screening and optimization. |
|---|---|
| Autores principais: | Guimarães, C. F. |
| Outros Autores: | Gasperini, L.; Ribeiro, R. S.; Carvalho, A. F.; Marques, A. P.; Reis, R. L. |
| Assunto: | 3D Gradients Cell-Material Interaction High-throughput screening Microfluidics |
| Ano: | 2020 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866876004942741504 |
|---|---|
| author | Guimarães, C. F. |
| author2 | Gasperini, L. Ribeiro, R. S. Carvalho, A. F. Marques, A. P. Reis, R. L. |
| author2_role | author author author author author |
| author_facet | Guimarães, C. F. Gasperini, L. Ribeiro, R. S. Carvalho, A. F. Marques, A. P. Reis, R. L. |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Guimarães, C. F.\"},{\"Person.name\":\"Gasperini, L.\"},{\"Person.name\":\"Ribeiro, R. S.\"},{\"Person.name\":\"Carvalho, A. F.\"},{\"Person.name\":\"Marques, A. P.\"},{\"Person.name\":\"Reis, R. L.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Guimarães, C. F. Gasperini, L. Ribeiro, R. S. Carvalho, A. F. Marques, A. P. Reis, R. L. |
| datacite.date.Accepted.fl_str_mv | 2020-06-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2021-07-01T06:00:20Z |
| datacite.date.embargoed.fl_str_mv | 2021-07-01T06:00:20Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | 3D Gradients Cell-Material Interaction High-throughput screening Microfluidics |
| datacite.titles.title.fl_str_mv | High-throughput fabrication of cell-laden 3D biomaterial gradients |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Guimarães, C. F. Gasperini, L. Ribeiro, R. S. Carvalho, A. F. Marques, A. P. Reis, R. L. |
| dc.date.Accepted.fl_str_mv | 2020-06-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2021-07-01T06:00:20Z |
| dc.date.embargoed.fl_str_mv | 2021-07-01T06:00:20Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/65800 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Royal Society of Chemistry |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | 3D Gradients Cell-Material Interaction High-throughput screening Microfluidics |
| dc.title.fl_str_mv | High-throughput fabrication of cell-laden 3D biomaterial gradients |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | High-throughput strategies for optimizing biomaterials to direct cellular behaviour are a fundamental need for propelling tissue engineering and regenerative medicine. In 2D, biomaterialâ s gradients have proven to be powerful platforms for simultaneously screening several surface conditions. However, their translation to 3D is yet limited to 1) exploiting light-based crosslinking and 2) non-sequential, single-gradient production. We built a microfluidic platform that allows distinct hydrogel precursors, as fluids, to be gradually mixed and crosslinked into 3D gradient fibres. Herein, we report how this system can be used for the sequential fabrication of independent cell-laden libraries with gradients of polymer concentration, non-adhesive/adhesive materials and both ionic and light crosslinking mechanisms. Automated image analysis of hundreds of single-cell events as a function of position yielded trends and pinpointed best-fit conditions based on cell shape, adhesion, proliferation and triggering of stromal/stem cell differentiation. We deliver a simple, versatile, and complete approach towards fully high-throughput 3D gradient fabrication for cell/material screening and optimization. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/be228d08-c83f-447b-9b55-ff21bfd0b088/download |
| id | rum_2a8d73fcc554ae0dcf3db0df93a6c54c |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/65800 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/65800 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Guimarães, C. F. Gasperini, L. Ribeiro, R. S. Carvalho, A. F. Marques, A. P. Reis, R. L. |
| publishDate | 2020 |
| publisher.none.fl_str_mv | Royal Society of Chemistry |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engRoyal Society of ChemistryporHigh-throughput strategies for optimizing biomaterials to direct cellular behaviour are a fundamental need for propelling tissue engineering and regenerative medicine. In 2D, biomaterialâ s gradients have proven to be powerful platforms for simultaneously screening several surface conditions. However, their translation to 3D is yet limited to 1) exploiting light-based crosslinking and 2) non-sequential, single-gradient production. We built a microfluidic platform that allows distinct hydrogel precursors, as fluids, to be gradually mixed and crosslinked into 3D gradient fibres. Herein, we report how this system can be used for the sequential fabrication of independent cell-laden libraries with gradients of polymer concentration, non-adhesive/adhesive materials and both ionic and light crosslinking mechanisms. Automated image analysis of hundreds of single-cell events as a function of position yielded trends and pinpointed best-fit conditions based on cell shape, adhesion, proliferation and triggering of stromal/stem cell differentiation. We deliver a simple, versatile, and complete approach towards fully high-throughput 3D gradient fabrication for cell/material screening and optimization.application/pdfporHigh-throughput fabrication of cell-laden 3D biomaterial gradientsGuimarães, C. F.Gasperini, L.Ribeiro, R. S.Carvalho, A. F.Marques, A. P.Reis, R. L.HostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf2051-6355DOIIsPartOf10.1039/D0MH00818D2021-07-01T06:00:20Z2020-062020-062020-06-30T08:39:07Z2020-06-01T00:00:00ZHandlehttps://hdl.handle.net/1822/65800http://purl.org/coar/access_right/c_abf2open access3D GradientsCell-Material InteractionHigh-throughput screeningMicrofluidics2315066 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/be228d08-c83f-447b-9b55-ff21bfd0b088/download |
| spellingShingle | High-throughput fabrication of cell-laden 3D biomaterial gradients Guimarães, C. F. 3D Gradients Cell-Material Interaction High-throughput screening Microfluidics |
| status | SINGLETON |
| subject.fl_str_mv | 3D Gradients Cell-Material Interaction High-throughput screening Microfluidics |
| title | High-throughput fabrication of cell-laden 3D biomaterial gradients |
| title_full | High-throughput fabrication of cell-laden 3D biomaterial gradients |
| title_fullStr | High-throughput fabrication of cell-laden 3D biomaterial gradients |
| title_full_unstemmed | High-throughput fabrication of cell-laden 3D biomaterial gradients |
| title_short | High-throughput fabrication of cell-laden 3D biomaterial gradients |
| title_sort | High-throughput fabrication of cell-laden 3D biomaterial gradients |
| topic | 3D Gradients Cell-Material Interaction High-throughput screening Microfluidics |
| topic_facet | 3D Gradients Cell-Material Interaction High-throughput screening Microfluidics |
| url | https://hdl.handle.net/1822/65800 |
| visible | 1 |