Publicação
Evaluation of newly synthesised benzo[a]phenoxazinium chlorides as fluorescent probes using Saccharomyces cerevisiae as model organism
| Resumo: | The potential as fluorescent probes of two recently synthesised water-soluble benzo[a]phenoxazinium salts, mono- or dissubstituted with carboxylic ester groups as terminal of propyl substituents at the amine of position 9, were studied by fluorescence microscopy employing intact, live cells. With the aim of assessing the compounds cellular staining pattern and potential specificity, co-localization experiments were performed using yeast mutants expressing green fluorescent protein (GFP)-tagged proteins that localize to specific intracellular location/organelles. It was found that both compounds display a very high affinity for the vacuolar membrane, being the dissubstituted compound especially important due to its higher fluorescence at lower, less toxic concentrations. |
|---|---|
| Autores principais: | Leitão, Maria Inês |
| Outros Autores: | Raju, B. Rama; Sousa, Maria João; Gonçalves, M. Sameiro T. |
| Assunto: | NIR fluorescent probes Nile Blue Benzo[a]phenoxazine derivatives Fluorescence microscopy |
| Ano: | 2014 |
| País: | Portugal |
| Tipo de documento: | comunicação em conferência |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866271012850499584 |
|---|---|
| author | Leitão, Maria Inês |
| author2 | Raju, B. Rama Sousa, Maria João Gonçalves, M. Sameiro T. |
| author2_role | author author author |
| author_facet | Leitão, Maria Inês Raju, B. Rama Sousa, Maria João Gonçalves, M. Sameiro T. |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Leitão, Maria Inês\"},{\"Person.name\":\"Raju, B. Rama\"},{\"Person.name\":\"Sousa, Maria João\"},{\"Person.name\":\"Gonçalves, M. Sameiro T.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Leitão, Maria Inês Raju, B. Rama Sousa, Maria João Gonçalves, M. Sameiro T. |
| datacite.date.Accepted.fl_str_mv | 2014-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2014-12-04T12:39:59Z |
| datacite.date.embargoed.fl_str_mv | 2014-12-04T12:39:59Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | NIR fluorescent probes Nile Blue Benzo[a]phenoxazine derivatives Fluorescence microscopy |
| datacite.titles.title.fl_str_mv | Evaluation of newly synthesised benzo[a]phenoxazinium chlorides as fluorescent probes using Saccharomyces cerevisiae as model organism |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Leitão, Maria Inês Raju, B. Rama Sousa, Maria João Gonçalves, M. Sameiro T. |
| dc.date.Accepted.fl_str_mv | 2014-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2014-12-04T12:39:59Z |
| dc.date.embargoed.fl_str_mv | 2014-12-04T12:39:59Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/31614 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | NIR fluorescent probes Nile Blue Benzo[a]phenoxazine derivatives Fluorescence microscopy |
| dc.title.fl_str_mv | Evaluation of newly synthesised benzo[a]phenoxazinium chlorides as fluorescent probes using Saccharomyces cerevisiae as model organism |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_5794 |
| description | The potential as fluorescent probes of two recently synthesised water-soluble benzo[a]phenoxazinium salts, mono- or dissubstituted with carboxylic ester groups as terminal of propyl substituents at the amine of position 9, were studied by fluorescence microscopy employing intact, live cells. With the aim of assessing the compounds cellular staining pattern and potential specificity, co-localization experiments were performed using yeast mutants expressing green fluorescent protein (GFP)-tagged proteins that localize to specific intracellular location/organelles. It was found that both compounds display a very high affinity for the vacuolar membrane, being the dissubstituted compound especially important due to its higher fluorescence at lower, less toxic concentrations. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | conferencePaper |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/9d226558-9c18-4ee2-9846-be34c6657d79/download |
| id | rum_2ade6087ca3b6c2c6005e40335c8feb4 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/31614 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/31614 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Leitão, Maria Inês Raju, B. Rama Sousa, Maria João Gonçalves, M. Sameiro T. |
| publishDate | 2014 |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engporThe potential as fluorescent probes of two recently synthesised water-soluble benzo[a]phenoxazinium salts, mono- or dissubstituted with carboxylic ester groups as terminal of propyl substituents at the amine of position 9, were studied by fluorescence microscopy employing intact, live cells. With the aim of assessing the compounds cellular staining pattern and potential specificity, co-localization experiments were performed using yeast mutants expressing green fluorescent protein (GFP)-tagged proteins that localize to specific intracellular location/organelles. It was found that both compounds display a very high affinity for the vacuolar membrane, being the dissubstituted compound especially important due to its higher fluorescence at lower, less toxic concentrations.application/pdfporEvaluation of newly synthesised benzo[a]phenoxazinium chlorides as fluorescent probes using Saccharomyces cerevisiae as model organismLeitão, Maria InêsRaju, B. RamaSousa, Maria JoãoGonçalves, M. Sameiro T.HostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptDOIIsPartOf10.3390/ecsoc-18-b0212014-12-04T12:39:59Z20142014-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/31614http://purl.org/coar/access_right/c_16ecrestricted accessNIR fluorescent probesNile BlueBenzo[a]phenoxazine derivativesFluorescence microscopy245914 bytesother research producthttp://purl.org/coar/resource_type/c_5794conference paperhttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/9d226558-9c18-4ee2-9846-be34c6657d79/download |
| spellingShingle | Evaluation of newly synthesised benzo[a]phenoxazinium chlorides as fluorescent probes using Saccharomyces cerevisiae as model organism Leitão, Maria Inês NIR fluorescent probes Nile Blue Benzo[a]phenoxazine derivatives Fluorescence microscopy |
| status | SINGLETON |
| subject.fl_str_mv | NIR fluorescent probes Nile Blue Benzo[a]phenoxazine derivatives Fluorescence microscopy |
| title | Evaluation of newly synthesised benzo[a]phenoxazinium chlorides as fluorescent probes using Saccharomyces cerevisiae as model organism |
| title_full | Evaluation of newly synthesised benzo[a]phenoxazinium chlorides as fluorescent probes using Saccharomyces cerevisiae as model organism |
| title_fullStr | Evaluation of newly synthesised benzo[a]phenoxazinium chlorides as fluorescent probes using Saccharomyces cerevisiae as model organism |
| title_full_unstemmed | Evaluation of newly synthesised benzo[a]phenoxazinium chlorides as fluorescent probes using Saccharomyces cerevisiae as model organism |
| title_short | Evaluation of newly synthesised benzo[a]phenoxazinium chlorides as fluorescent probes using Saccharomyces cerevisiae as model organism |
| title_sort | Evaluation of newly synthesised benzo[a]phenoxazinium chlorides as fluorescent probes using Saccharomyces cerevisiae as model organism |
| topic | NIR fluorescent probes Nile Blue Benzo[a]phenoxazine derivatives Fluorescence microscopy |
| topic_facet | NIR fluorescent probes Nile Blue Benzo[a]phenoxazine derivatives Fluorescence microscopy |
| url | https://hdl.handle.net/1822/31614 |
| visible | 1 |