Publication
Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains
| Summary: | This work presents the exploitation of waste industrial by-products as raw materials for the production of microbial lipids in engineered strains of the filamentous fungus Ashbya gossypii. A lipogenic xylose-utilizing strain was used to apply a metabolic engineering approach aiming at relieving regulatory mechanisms to further increase the biosynthesis of lipids. Three genomic manipulations were applied: the overexpression of a feedback resistant form of the acetyl-CoA carboxylase enzyme; the expression of a truncated form of Mga2, a regulator of the main 9 desaturase gene; and the overexpression of an additional copy of DGA1 that codes for diacylglycerol acyltransferase. The performance of the engineered strain was evaluated in culture media containing mixed formulations of corn-cob hydrolysates, sugarcane molasses or crude glycerol. Our results demonstrate the efficiency of the engineered strains, which were able to accumulate about 40% of cell dry weight (CDW) in lipid content using organic industrial wastes as feedstocks. |
|---|---|
| Main Authors: | Díaz-Fernández, David |
| Other Authors: | Aguiar, Tatiana Quinta; Martín, Victoria Isabel; Romani, Aloia Perez; Silva, Rui; Domingues, Lucília; Revuelta, José Luis; Jiménez, Alberto |
| Subject: | Ashbya gossypii microbial lipids regulation lignocellulosic hydrolysates xylose molasses glycerol |
| Year: | 2019 |
| Country: | Portugal |
| Document type: | article |
| Access type: | open access |
| Associated institution: | Universidade do Minho |
| Language: | English |
| Origin: | RepositóriUM - Universidade do Minho |
| _version_ | 1867438315254317056 |
|---|---|
| author | Díaz-Fernández, David |
| author2 | Aguiar, Tatiana Quinta Martín, Victoria Isabel Romani, Aloia Perez Silva, Rui Domingues, Lucília Revuelta, José Luis Jiménez, Alberto |
| author2_role | author author author author author author author |
| author_facet | Díaz-Fernández, David Aguiar, Tatiana Quinta Martín, Victoria Isabel Romani, Aloia Perez Silva, Rui Domingues, Lucília Revuelta, José Luis Jiménez, Alberto |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Díaz-Fernández, David\"},{\"Person.name\":\"Aguiar, Tatiana Quinta\"},{\"Person.name\":\"Martín, Victoria Isabel\"},{\"Person.name\":\"Romani, Aloia Perez\"},{\"Person.name\":\"Silva, Rui\"},{\"Person.name\":\"Domingues, Lucília\"},{\"Person.name\":\"Revuelta, José Luis\"},{\"Person.name\":\"Jiménez, Alberto\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Díaz-Fernández, David Aguiar, Tatiana Quinta Martín, Victoria Isabel Romani, Aloia Perez Silva, Rui Domingues, Lucília Revuelta, José Luis Jiménez, Alberto |
| datacite.date.Accepted.fl_str_mv | 2019-12-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2019-09-09T08:14:48Z |
| datacite.date.embargoed.fl_str_mv | 2019-09-09T08:14:48Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Ashbya gossypii microbial lipids regulation lignocellulosic hydrolysates xylose molasses glycerol |
| datacite.titles.title.fl_str_mv | Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Díaz-Fernández, David Aguiar, Tatiana Quinta Martín, Victoria Isabel Romani, Aloia Perez Silva, Rui Domingues, Lucília Revuelta, José Luis Jiménez, Alberto |
| dc.date.Accepted.fl_str_mv | 2019-12-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2019-09-09T08:14:48Z |
| dc.date.embargoed.fl_str_mv | 2019-09-09T08:14:48Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/61335 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by-nd/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.rights.rights.copyright.fl_str_mv | openAccess |
| dc.subject.none.fl_str_mv | Ashbya gossypii microbial lipids regulation lignocellulosic hydrolysates xylose molasses glycerol |
| dc.title.fl_str_mv | Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | This work presents the exploitation of waste industrial by-products as raw materials for the production of microbial lipids in engineered strains of the filamentous fungus Ashbya gossypii. A lipogenic xylose-utilizing strain was used to apply a metabolic engineering approach aiming at relieving regulatory mechanisms to further increase the biosynthesis of lipids. Three genomic manipulations were applied: the overexpression of a feedback resistant form of the acetyl-CoA carboxylase enzyme; the expression of a truncated form of Mga2, a regulator of the main 9 desaturase gene; and the overexpression of an additional copy of DGA1 that codes for diacylglycerol acyltransferase. The performance of the engineered strain was evaluated in culture media containing mixed formulations of corn-cob hydrolysates, sugarcane molasses or crude glycerol. Our results demonstrate the efficiency of the engineered strains, which were able to accumulate about 40% of cell dry weight (CDW) in lipid content using organic industrial wastes as feedstocks. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/7f018e6e-2144-4eaf-bc08-439aa405b4c1/download |
| id | rum_2dfb5bca169519fe0cf0b92fc87c8f08 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/61335 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/61335 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Díaz-Fernández, David Aguiar, Tatiana Quinta Martín, Victoria Isabel Romani, Aloia Perez Silva, Rui Domingues, Lucília Revuelta, José Luis Jiménez, Alberto |
| publishDate | 2019 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engElsevierporThis work presents the exploitation of waste industrial by-products as raw materials for the production of microbial lipids in engineered strains of the filamentous fungus Ashbya gossypii. A lipogenic xylose-utilizing strain was used to apply a metabolic engineering approach aiming at relieving regulatory mechanisms to further increase the biosynthesis of lipids. Three genomic manipulations were applied: the overexpression of a feedback resistant form of the acetyl-CoA carboxylase enzyme; the expression of a truncated form of Mga2, a regulator of the main 9 desaturase gene; and the overexpression of an additional copy of DGA1 that codes for diacylglycerol acyltransferase. The performance of the engineered strain was evaluated in culture media containing mixed formulations of corn-cob hydrolysates, sugarcane molasses or crude glycerol. Our results demonstrate the efficiency of the engineered strains, which were able to accumulate about 40% of cell dry weight (CDW) in lipid content using organic industrial wastes as feedstocks.application/pdfporMicrobial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strainsDíaz-Fernández, DavidAguiar, Tatiana QuintaMartín, Victoria IsabelRomani, Aloia PerezSilva, RuiDomingues, LucíliaRevuelta, José LuisJiménez, AlbertoHostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONDíaz-Fernández, David; Aguiar, Tatiana Q.; Martín, Victoria Isabel; Romaní, Aloia; Silva, Rui; Domingues, Lucília; Revuelta, José Luis; Jiménez, Alberto, Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains. Bioresource Technology, 293(122054), 2019PMID31487616ISSNIsPartOf0960-8524EISSNIsPartOf0960-8524DOIIsPartOf10.1016/j.biortech.2019.1220542019-09-09T08:14:48Z2019-122019-09-08T09:57:40Z2019-12-01T00:00:00ZHandlehttps://hdl.handle.net/1822/61335http://purl.org/coar/access_right/c_abf2open accessAshbya gossypiimicrobial lipidsregulationlignocellulosic hydrolysatesxylosemolassesglycerol1204788 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2019-12http://creativecommons.org/licenses/by-nd/4.0/openAccesshttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/7f018e6e-2144-4eaf-bc08-439aa405b4c1/download |
| spellingShingle | Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains Díaz-Fernández, David Ashbya gossypii microbial lipids regulation lignocellulosic hydrolysates xylose molasses glycerol |
| status | SINGLETON |
| subject.fl_str_mv | Ashbya gossypii microbial lipids regulation lignocellulosic hydrolysates xylose molasses glycerol |
| title | Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains |
| title_full | Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains |
| title_fullStr | Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains |
| title_full_unstemmed | Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains |
| title_short | Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains |
| title_sort | Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains |
| topic | Ashbya gossypii microbial lipids regulation lignocellulosic hydrolysates xylose molasses glycerol |
| topic_facet | Ashbya gossypii microbial lipids regulation lignocellulosic hydrolysates xylose molasses glycerol |
| url | https://hdl.handle.net/1822/61335 |
| visible | 1 |