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Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains

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Resumo:This work presents the exploitation of waste industrial by-products as raw materials for the production of microbial lipids in engineered strains of the filamentous fungus Ashbya gossypii. A lipogenic xylose-utilizing strain was used to apply a metabolic engineering approach aiming at relieving regulatory mechanisms to further increase the biosynthesis of lipids. Three genomic manipulations were applied: the overexpression of a feedback resistant form of the acetyl-CoA carboxylase enzyme; the expression of a truncated form of Mga2, a regulator of the main 9 desaturase gene; and the overexpression of an additional copy of DGA1 that codes for diacylglycerol acyltransferase. The performance of the engineered strain was evaluated in culture media containing mixed formulations of corn-cob hydrolysates, sugarcane molasses or crude glycerol. Our results demonstrate the efficiency of the engineered strains, which were able to accumulate about 40% of cell dry weight (CDW) in lipid content using organic industrial wastes as feedstocks.
Autores principais:Díaz-Fernández, David
Outros Autores:Aguiar, Tatiana Quinta; Martín, Victoria Isabel; Romani, Aloia Perez; Silva, Rui; Domingues, Lucília; Revuelta, José Luis; Jiménez, Alberto
Assunto:Ashbya gossypii microbial lipids regulation lignocellulosic hydrolysates xylose molasses glycerol
Ano:2019
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho

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