Publicação
Evaluation of Bronchoalveolar Lavage Fluid Cytokines as Biomarkers for Invasive Pulmonary Aspergillosis in At-Risk Patients
| Resumo: | Background: Invasive pulmonary aspergillosis (IPA) is an infection that primarily affects immunocompromised hosts, including hematological patients and stem-cell transplant recipients. The diagnosis of IPA remains challenging, making desirable the availability of new specific biomarkers. High-throughput methods now allow us to interrogate the immune system for multiple markers of inflammation with enhanced resolution.Methods: To determine whether a signature of alveolar cytokines could be associated with the development of IPA and used as a diagnostic biomarker, we performed a nested case-control study involving 113 patients at-risk.Results: Among the 32 analytes tested, IL-beta, IL-6, IL-8, IL-17A, IL-23, and TNF a were significantly increased among patients with IPA, defining two clusters able to accurately differentiate cases of infection from controls. Genetic variants previously reported to confer increased risk of IPA compromised the production of specific cytokines and impaired their discriminatory potential toward infection. Collectively, our data indicated that IL-8 was the best performing cytokine, with alveolar levels >= 904 pg/mL predicting IPA with elevated sensitivity (90%), specificity (73%), and negative predictive value (88%).Conclusions: These findings highlight the existence of a specific profile of alveolar cytokines, with IL-8 being the dominant discriminator, whichmight be useful in supporting current diagnostic approaches for IPA. |
|---|---|
| Autores principais: | Gonçalves, Samuel Martins |
| Outros Autores: | Lagrou, Katrien; Rodrigues, Claudia S.; Campos, Claudia F.; Bernal-Martinez, Leticia; Rodrigues, Fernando José dos Santos; Silvestre, Ricardo Jorge Leal; Alcazar-Fuoli, Laura; Maertens, Johan A.; Cunha, Cristina; Carvalho, Agostinho |
| Assunto: | Invasive pulmonary aspergillosis Biomarkers Cytokines/chemokines Bronchoalveolar lavage Fungal diagnostics |
| Ano: | 2017 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866876315127250944 |
|---|---|
| author | Gonçalves, Samuel Martins |
| author2 | Lagrou, Katrien Rodrigues, Claudia S. Campos, Claudia F. Bernal-Martinez, Leticia Rodrigues, Fernando José dos Santos Silvestre, Ricardo Jorge Leal Alcazar-Fuoli, Laura Maertens, Johan A. Cunha, Cristina Carvalho, Agostinho |
| author2_role | author author author author author author author author author author |
| author_facet | Gonçalves, Samuel Martins Lagrou, Katrien Rodrigues, Claudia S. Campos, Claudia F. Bernal-Martinez, Leticia Rodrigues, Fernando José dos Santos Silvestre, Ricardo Jorge Leal Alcazar-Fuoli, Laura Maertens, Johan A. Cunha, Cristina Carvalho, Agostinho |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Gonçalves, Samuel Martins\"},{\"Person.name\":\"Lagrou, Katrien\"},{\"Person.name\":\"Rodrigues, Claudia S.\"},{\"Person.name\":\"Campos, Claudia F.\"},{\"Person.name\":\"Bernal-Martinez, Leticia\"},{\"Person.name\":\"Rodrigues, Fernando José dos Santos\"},{\"Person.name\":\"Silvestre, Ricardo Jorge Leal\"},{\"Person.name\":\"Alcazar-Fuoli, Laura\"},{\"Person.name\":\"Maertens, Johan A.\"},{\"Person.name\":\"Cunha, Cristina\"},{\"Person.name\":\"Carvalho, Agostinho\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Gonçalves, Samuel Martins Lagrou, Katrien Rodrigues, Claudia S. Campos, Claudia F. Bernal-Martinez, Leticia Rodrigues, Fernando José dos Santos Silvestre, Ricardo Jorge Leal Alcazar-Fuoli, Laura Maertens, Johan A. Cunha, Cristina Carvalho, Agostinho |
| datacite.date.Accepted.fl_str_mv | 2017-11-29T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-02-09T12:05:58Z |
| datacite.date.embargoed.fl_str_mv | 2018-02-09T12:05:58Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Invasive pulmonary aspergillosis Biomarkers Cytokines/chemokines Bronchoalveolar lavage Fungal diagnostics |
| datacite.titles.title.fl_str_mv | Evaluation of Bronchoalveolar Lavage Fluid Cytokines as Biomarkers for Invasive Pulmonary Aspergillosis in At-Risk Patients |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Gonçalves, Samuel Martins Lagrou, Katrien Rodrigues, Claudia S. Campos, Claudia F. Bernal-Martinez, Leticia Rodrigues, Fernando José dos Santos Silvestre, Ricardo Jorge Leal Alcazar-Fuoli, Laura Maertens, Johan A. Cunha, Cristina Carvalho, Agostinho |
| dc.date.Accepted.fl_str_mv | 2017-11-29T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-02-09T12:05:58Z |
| dc.date.embargoed.fl_str_mv | 2018-02-09T12:05:58Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/50276 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Frontiers Media |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Invasive pulmonary aspergillosis Biomarkers Cytokines/chemokines Bronchoalveolar lavage Fungal diagnostics |
| dc.title.fl_str_mv | Evaluation of Bronchoalveolar Lavage Fluid Cytokines as Biomarkers for Invasive Pulmonary Aspergillosis in At-Risk Patients |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Background: Invasive pulmonary aspergillosis (IPA) is an infection that primarily affects immunocompromised hosts, including hematological patients and stem-cell transplant recipients. The diagnosis of IPA remains challenging, making desirable the availability of new specific biomarkers. High-throughput methods now allow us to interrogate the immune system for multiple markers of inflammation with enhanced resolution.Methods: To determine whether a signature of alveolar cytokines could be associated with the development of IPA and used as a diagnostic biomarker, we performed a nested case-control study involving 113 patients at-risk.Results: Among the 32 analytes tested, IL-beta, IL-6, IL-8, IL-17A, IL-23, and TNF a were significantly increased among patients with IPA, defining two clusters able to accurately differentiate cases of infection from controls. Genetic variants previously reported to confer increased risk of IPA compromised the production of specific cytokines and impaired their discriminatory potential toward infection. Collectively, our data indicated that IL-8 was the best performing cytokine, with alveolar levels >= 904 pg/mL predicting IPA with elevated sensitivity (90%), specificity (73%), and negative predictive value (88%).Conclusions: These findings highlight the existence of a specific profile of alveolar cytokines, with IL-8 being the dominant discriminator, whichmight be useful in supporting current diagnostic approaches for IPA. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/9c653ea7-7296-4186-8401-4eeefa28a3ed/download |
| id | rum_34cece6df3ce233bdebd4a2cdf06002f |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/50276 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/50276 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Gonçalves, Samuel Martins Lagrou, Katrien Rodrigues, Claudia S. Campos, Claudia F. Bernal-Martinez, Leticia Rodrigues, Fernando José dos Santos Silvestre, Ricardo Jorge Leal Alcazar-Fuoli, Laura Maertens, Johan A. Cunha, Cristina Carvalho, Agostinho |
| publishDate | 2017 |
| publisher.none.fl_str_mv | Frontiers Media |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engFrontiers MediaporBackground: Invasive pulmonary aspergillosis (IPA) is an infection that primarily affects immunocompromised hosts, including hematological patients and stem-cell transplant recipients. The diagnosis of IPA remains challenging, making desirable the availability of new specific biomarkers. High-throughput methods now allow us to interrogate the immune system for multiple markers of inflammation with enhanced resolution.Methods: To determine whether a signature of alveolar cytokines could be associated with the development of IPA and used as a diagnostic biomarker, we performed a nested case-control study involving 113 patients at-risk.Results: Among the 32 analytes tested, IL-beta, IL-6, IL-8, IL-17A, IL-23, and TNF a were significantly increased among patients with IPA, defining two clusters able to accurately differentiate cases of infection from controls. Genetic variants previously reported to confer increased risk of IPA compromised the production of specific cytokines and impaired their discriminatory potential toward infection. Collectively, our data indicated that IL-8 was the best performing cytokine, with alveolar levels >= 904 pg/mL predicting IPA with elevated sensitivity (90%), specificity (73%), and negative predictive value (88%).Conclusions: These findings highlight the existence of a specific profile of alveolar cytokines, with IL-8 being the dominant discriminator, whichmight be useful in supporting current diagnostic approaches for IPA.application/pdfporEvaluation of Bronchoalveolar Lavage Fluid Cytokines as Biomarkers for Invasive Pulmonary Aspergillosis in At-Risk PatientsGonçalves, Samuel MartinsLagrou, KatrienRodrigues, Claudia S.Campos, Claudia F.Bernal-Martinez, LeticiaRodrigues, Fernando José dos SantosSilvestre, Ricardo Jorge LealAlcazar-Fuoli, LauraMaertens, Johan A.Cunha, CristinaCarvalho, AgostinhoHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf1664-302XDOIIsPartOf10.3389/fmicb.2017.023622018-02-09T12:05:58Z2017-11-292018-01-16T16:30:56Z2017-11-29T00:00:00ZHandlehttps://hdl.handle.net/1822/50276http://purl.org/coar/access_right/c_abf2open accessInvasive pulmonary aspergillosisBiomarkersCytokines/chemokinesBronchoalveolar lavageFungal diagnostics4474140 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/9c653ea7-7296-4186-8401-4eeefa28a3ed/download |
| spellingShingle | Evaluation of Bronchoalveolar Lavage Fluid Cytokines as Biomarkers for Invasive Pulmonary Aspergillosis in At-Risk Patients Gonçalves, Samuel Martins Invasive pulmonary aspergillosis Biomarkers Cytokines/chemokines Bronchoalveolar lavage Fungal diagnostics |
| status | SINGLETON |
| subject.fl_str_mv | Invasive pulmonary aspergillosis Biomarkers Cytokines/chemokines Bronchoalveolar lavage Fungal diagnostics |
| title | Evaluation of Bronchoalveolar Lavage Fluid Cytokines as Biomarkers for Invasive Pulmonary Aspergillosis in At-Risk Patients |
| title_full | Evaluation of Bronchoalveolar Lavage Fluid Cytokines as Biomarkers for Invasive Pulmonary Aspergillosis in At-Risk Patients |
| title_fullStr | Evaluation of Bronchoalveolar Lavage Fluid Cytokines as Biomarkers for Invasive Pulmonary Aspergillosis in At-Risk Patients |
| title_full_unstemmed | Evaluation of Bronchoalveolar Lavage Fluid Cytokines as Biomarkers for Invasive Pulmonary Aspergillosis in At-Risk Patients |
| title_short | Evaluation of Bronchoalveolar Lavage Fluid Cytokines as Biomarkers for Invasive Pulmonary Aspergillosis in At-Risk Patients |
| title_sort | Evaluation of Bronchoalveolar Lavage Fluid Cytokines as Biomarkers for Invasive Pulmonary Aspergillosis in At-Risk Patients |
| topic | Invasive pulmonary aspergillosis Biomarkers Cytokines/chemokines Bronchoalveolar lavage Fungal diagnostics |
| topic_facet | Invasive pulmonary aspergillosis Biomarkers Cytokines/chemokines Bronchoalveolar lavage Fungal diagnostics |
| url | https://hdl.handle.net/1822/50276 |
| visible | 1 |