Publicação
Flow cytometry for age assessment of a yeast population and its application in beer fermentations
| Resumo: | An expeditious method of yeast age estimation was developed based on selective bud scar staining (Alexa Fluor 488-labelled wheat-germ agglutinin) and subsequent fluorescence intensity measurement by flow cytometry. The calibration curve resulting from the cytometric determination of average bud scar fluorescence intensities vs. microscopically counted average bud scar numbers of the same cell populations showed a good correlation and allowed routine cell age estimation by flow cytometry. The developed method was applied for yeast age control in traditional batch and continuous beer fermentations. At the pitching rates used in industrial beer fermentations, our results support former findings by locating a gradient of increasing yeast age from the top to the bottom zone of the fermenter cone. The results also indicate that in continuous beer fermentation, the increasing bud scar fluorescence of immobilized cells could help to schedule the replacement of aged biomass, prior to loss of viability or deterioration of process performance and product quality. |
|---|---|
| Autores principais: | Kuřec, Michal |
| Outros Autores: | Baszczyňski, Martin; Lehnert, Radek; Mota, André; Teixeira, J. A.; Brányik, Tomáš |
| Assunto: | Aging Beer Bud scar Flow cytometry Staining Yeast |
| Ano: | 2009 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866878110080696320 |
|---|---|
| author | Kuřec, Michal |
| author2 | Baszczyňski, Martin Lehnert, Radek Mota, André Teixeira, J. A. Brányik, Tomáš |
| author2_role | author author author author author |
| author_facet | Kuřec, Michal Baszczyňski, Martin Lehnert, Radek Mota, André Teixeira, J. A. Brányik, Tomáš |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Kuřec, Michal\"},{\"Person.name\":\"Baszczyňski, Martin\"},{\"Person.name\":\"Lehnert, Radek\"},{\"Person.name\":\"Mota, André\"},{\"Person.name\":\"Teixeira, J. A.\"},{\"Person.name\":\"Brányik, Tomáš\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Kuřec, Michal Baszczyňski, Martin Lehnert, Radek Mota, André Teixeira, J. A. Brányik, Tomáš |
| datacite.date.Accepted.fl_str_mv | 2009-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2010-01-26T15:50:30Z |
| datacite.date.embargoed.fl_str_mv | 2010-01-26T15:50:30Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Aging Beer Bud scar Flow cytometry Staining Yeast |
| datacite.titles.title.fl_str_mv | Flow cytometry for age assessment of a yeast population and its application in beer fermentations |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Kuřec, Michal Baszczyňski, Martin Lehnert, Radek Mota, André Teixeira, J. A. Brányik, Tomáš |
| dc.date.Accepted.fl_str_mv | 2009-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2010-01-26T15:50:30Z |
| dc.date.embargoed.fl_str_mv | 2010-01-26T15:50:30Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/10194 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Institute of Brewing & Distilling |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Aging Beer Bud scar Flow cytometry Staining Yeast |
| dc.title.fl_str_mv | Flow cytometry for age assessment of a yeast population and its application in beer fermentations |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | An expeditious method of yeast age estimation was developed based on selective bud scar staining (Alexa Fluor 488-labelled wheat-germ agglutinin) and subsequent fluorescence intensity measurement by flow cytometry. The calibration curve resulting from the cytometric determination of average bud scar fluorescence intensities vs. microscopically counted average bud scar numbers of the same cell populations showed a good correlation and allowed routine cell age estimation by flow cytometry. The developed method was applied for yeast age control in traditional batch and continuous beer fermentations. At the pitching rates used in industrial beer fermentations, our results support former findings by locating a gradient of increasing yeast age from the top to the bottom zone of the fermenter cone. The results also indicate that in continuous beer fermentation, the increasing bud scar fluorescence of immobilized cells could help to schedule the replacement of aged biomass, prior to loss of viability or deterioration of process performance and product quality. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/a902e6ab-cc7b-426a-8f10-ca5786149309/download |
| id | rum_408cf8183a7940d75ce59cd2023b68ba |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/10194 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/10194 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Kuřec, Michal Baszczyňski, Martin Lehnert, Radek Mota, André Teixeira, J. A. Brányik, Tomáš |
| publishDate | 2009 |
| publisher.none.fl_str_mv | Institute of Brewing & Distilling |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engInstitute of Brewing & DistillingporAn expeditious method of yeast age estimation was developed based on selective bud scar staining (Alexa Fluor 488-labelled wheat-germ agglutinin) and subsequent fluorescence intensity measurement by flow cytometry. The calibration curve resulting from the cytometric determination of average bud scar fluorescence intensities vs. microscopically counted average bud scar numbers of the same cell populations showed a good correlation and allowed routine cell age estimation by flow cytometry. The developed method was applied for yeast age control in traditional batch and continuous beer fermentations. At the pitching rates used in industrial beer fermentations, our results support former findings by locating a gradient of increasing yeast age from the top to the bottom zone of the fermenter cone. The results also indicate that in continuous beer fermentation, the increasing bud scar fluorescence of immobilized cells could help to schedule the replacement of aged biomass, prior to loss of viability or deterioration of process performance and product quality.application/pdfporFlow cytometry for age assessment of a yeast population and its application in beer fermentationsKuřec, MichalBaszczyňski, MartinLehnert, RadekMota, AndréTeixeira, J. A.Brányik, TomášHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf0046-9750DOIIsPartOf10.1002/j.2050-0416.2009.tb00377.x2010-01-26T15:50:30Z20092009-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/10194http://purl.org/coar/access_right/c_abf2open accessAgingBeerBud scarFlow cytometryStainingYeast570294 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/a902e6ab-cc7b-426a-8f10-ca5786149309/download |
| spellingShingle | Flow cytometry for age assessment of a yeast population and its application in beer fermentations Kuřec, Michal Aging Beer Bud scar Flow cytometry Staining Yeast |
| status | SINGLETON |
| subject.fl_str_mv | Aging Beer Bud scar Flow cytometry Staining Yeast |
| title | Flow cytometry for age assessment of a yeast population and its application in beer fermentations |
| title_full | Flow cytometry for age assessment of a yeast population and its application in beer fermentations |
| title_fullStr | Flow cytometry for age assessment of a yeast population and its application in beer fermentations |
| title_full_unstemmed | Flow cytometry for age assessment of a yeast population and its application in beer fermentations |
| title_short | Flow cytometry for age assessment of a yeast population and its application in beer fermentations |
| title_sort | Flow cytometry for age assessment of a yeast population and its application in beer fermentations |
| topic | Aging Beer Bud scar Flow cytometry Staining Yeast |
| topic_facet | Aging Beer Bud scar Flow cytometry Staining Yeast |
| url | https://hdl.handle.net/1822/10194 |
| visible | 1 |