Publicação
Toward a platform for the treatment of burns: An assessment of nanoemulsions vs. nanostructured lipid carriers loaded with curcumin
| Resumo: | Curcumin is a highly promising substance for treating burns, owing to its anti-inflammatory, antioxidant, antimicrobial, and wound-healing properties. However, its therapeutic use is restricted due to its hydrophobic nature and low bioavailability. This study was conducted to address these limitations; it developed and tested two types of lipid nanocarriers, namely nanoemulsions (NE-CUR) and nanostructured lipid carriers (NLC-CUR) loaded with curcumin, and aimed to identify the most suitable nanocarrier for skin burn treatment. The study evaluated various parameters, including physicochemical characteristics, stability, encapsulation efficiency, release, skin permeation, retention, cell viability, and antimicrobial activity. The results showed that both nanocarriers showed adequate size (~200 nm), polydispersity index (~0.25), and zeta potential (~>20 mV). They also showed good encapsulation efficiency (>90%) and remained stable for 120 days at different temperatures. In the release test, NE-CUR and NCL-CUR released 57.14% and 51.64% of curcumin, respectively, in 72 h. NE-CUR demonstrated better cutaneous permeation/retention in intact or scalded skin epidermis and dermis than NLC-CUR. The cell viability test showed no toxicity after treatment with NE-CUR and NLC-CUR up to 125 g/mL. Regarding microbial activity assays, free curcumin has activity against P. aeruginosa, reducing bacterial growth by 75% in 3 h. NE-CUR inhibited bacterial growth by 65% after 24 h, and the association with gentamicin had favorable results, while NLC-CUR showed a lower inhibition. The results demonstrated that NE-CUR is probably the most promising nanocarrier for treating burns. |
|---|---|
| Autores principais: | Araújo, Gabriela de Moraes Soares |
| Outros Autores: | Loureiro, Ana Isabel Sá; Rodrigues, Jamile Lima; Barros, Paula Alice Bezerra; Halicki, Priscila Cristina Bartolomeu; Ramos, Daniela Fernandes; Marinho, Marcelo Augusto Germani; Vaiss, Daniela Pastorim; Vaz, Gustavo Richter; Yurgel, Virginia Campello; Bidone, Juliana; Muccillo-Baisch, Ana Luiza; Hort, Mariana Appel; Cavaco-Paulo, Artur; Dora, Cristiana Lima |
| Assunto: | Burn Nanotechnology Curcumin Nanoemulsion Nanostructured lipid carrier |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866271058675367936 |
|---|---|
| author | Araújo, Gabriela de Moraes Soares |
| author2 | Loureiro, Ana Isabel Sá Rodrigues, Jamile Lima Barros, Paula Alice Bezerra Halicki, Priscila Cristina Bartolomeu Ramos, Daniela Fernandes Marinho, Marcelo Augusto Germani Vaiss, Daniela Pastorim Vaz, Gustavo Richter Yurgel, Virginia Campello Bidone, Juliana Muccillo-Baisch, Ana Luiza Hort, Mariana Appel Cavaco-Paulo, Artur Dora, Cristiana Lima |
| author2_role | author author author author author author author author author author author author author author |
| author_facet | Araújo, Gabriela de Moraes Soares Loureiro, Ana Isabel Sá Rodrigues, Jamile Lima Barros, Paula Alice Bezerra Halicki, Priscila Cristina Bartolomeu Ramos, Daniela Fernandes Marinho, Marcelo Augusto Germani Vaiss, Daniela Pastorim Vaz, Gustavo Richter Yurgel, Virginia Campello Bidone, Juliana Muccillo-Baisch, Ana Luiza Hort, Mariana Appel Cavaco-Paulo, Artur Dora, Cristiana Lima |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Araújo, Gabriela de Moraes Soares\"},{\"Person.name\":\"Loureiro, Ana Isabel Sá\"},{\"Person.name\":\"Rodrigues, Jamile Lima\"},{\"Person.name\":\"Barros, Paula Alice Bezerra\"},{\"Person.name\":\"Halicki, Priscila Cristina Bartolomeu\"},{\"Person.name\":\"Ramos, Daniela Fernandes\"},{\"Person.name\":\"Marinho, Marcelo Augusto Germani\"},{\"Person.name\":\"Vaiss, Daniela Pastorim\"},{\"Person.name\":\"Vaz, Gustavo Richter\"},{\"Person.name\":\"Yurgel, Virginia Campello\"},{\"Person.name\":\"Bidone, Juliana\"},{\"Person.name\":\"Muccillo-Baisch, Ana Luiza\"},{\"Person.name\":\"Hort, Mariana Appel\"},{\"Person.name\":\"Cavaco-Paulo, Artur\"},{\"Person.name\":\"Dora, Cristiana Lima\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Araújo, Gabriela de Moraes Soares Loureiro, Ana Isabel Sá Rodrigues, Jamile Lima Barros, Paula Alice Bezerra Halicki, Priscila Cristina Bartolomeu Ramos, Daniela Fernandes Marinho, Marcelo Augusto Germani Vaiss, Daniela Pastorim Vaz, Gustavo Richter Yurgel, Virginia Campello Bidone, Juliana Muccillo-Baisch, Ana Luiza Hort, Mariana Appel Cavaco-Paulo, Artur Dora, Cristiana Lima |
| datacite.date.Accepted.fl_str_mv | 2023-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2024-05-22T10:09:20Z |
| datacite.date.embargoed.fl_str_mv | 2024-05-22T10:09:20Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Burn Nanotechnology Curcumin Nanoemulsion Nanostructured lipid carrier |
| datacite.titles.title.fl_str_mv | Toward a platform for the treatment of burns: An assessment of nanoemulsions vs. nanostructured lipid carriers loaded with curcumin |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Araújo, Gabriela de Moraes Soares Loureiro, Ana Isabel Sá Rodrigues, Jamile Lima Barros, Paula Alice Bezerra Halicki, Priscila Cristina Bartolomeu Ramos, Daniela Fernandes Marinho, Marcelo Augusto Germani Vaiss, Daniela Pastorim Vaz, Gustavo Richter Yurgel, Virginia Campello Bidone, Juliana Muccillo-Baisch, Ana Luiza Hort, Mariana Appel Cavaco-Paulo, Artur Dora, Cristiana Lima |
| dc.date.Accepted.fl_str_mv | 2023-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2024-05-22T10:09:20Z |
| dc.date.embargoed.fl_str_mv | 2024-05-22T10:09:20Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/91451 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI AG |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Burn Nanotechnology Curcumin Nanoemulsion Nanostructured lipid carrier |
| dc.title.fl_str_mv | Toward a platform for the treatment of burns: An assessment of nanoemulsions vs. nanostructured lipid carriers loaded with curcumin |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Curcumin is a highly promising substance for treating burns, owing to its anti-inflammatory, antioxidant, antimicrobial, and wound-healing properties. However, its therapeutic use is restricted due to its hydrophobic nature and low bioavailability. This study was conducted to address these limitations; it developed and tested two types of lipid nanocarriers, namely nanoemulsions (NE-CUR) and nanostructured lipid carriers (NLC-CUR) loaded with curcumin, and aimed to identify the most suitable nanocarrier for skin burn treatment. The study evaluated various parameters, including physicochemical characteristics, stability, encapsulation efficiency, release, skin permeation, retention, cell viability, and antimicrobial activity. The results showed that both nanocarriers showed adequate size (~200 nm), polydispersity index (~0.25), and zeta potential (~>20 mV). They also showed good encapsulation efficiency (>90%) and remained stable for 120 days at different temperatures. In the release test, NE-CUR and NCL-CUR released 57.14% and 51.64% of curcumin, respectively, in 72 h. NE-CUR demonstrated better cutaneous permeation/retention in intact or scalded skin epidermis and dermis than NLC-CUR. The cell viability test showed no toxicity after treatment with NE-CUR and NLC-CUR up to 125 g/mL. Regarding microbial activity assays, free curcumin has activity against P. aeruginosa, reducing bacterial growth by 75% in 3 h. NE-CUR inhibited bacterial growth by 65% after 24 h, and the association with gentamicin had favorable results, while NLC-CUR showed a lower inhibition. The results demonstrated that NE-CUR is probably the most promising nanocarrier for treating burns. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/d8f40031-9e76-4321-b55a-8f8706c57b06/download |
| id | rum_47243d94602fa3bcd341d8675d2ee767 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/91451 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/91451 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Araújo, Gabriela de Moraes Soares Loureiro, Ana Isabel Sá Rodrigues, Jamile Lima Barros, Paula Alice Bezerra Halicki, Priscila Cristina Bartolomeu Ramos, Daniela Fernandes Marinho, Marcelo Augusto Germani Vaiss, Daniela Pastorim Vaz, Gustavo Richter Yurgel, Virginia Campello Bidone, Juliana Muccillo-Baisch, Ana Luiza Hort, Mariana Appel Cavaco-Paulo, Artur Dora, Cristiana Lima |
| publishDate | 2023 |
| publisher.none.fl_str_mv | MDPI AG |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engMDPI AGporCurcumin is a highly promising substance for treating burns, owing to its anti-inflammatory, antioxidant, antimicrobial, and wound-healing properties. However, its therapeutic use is restricted due to its hydrophobic nature and low bioavailability. This study was conducted to address these limitations; it developed and tested two types of lipid nanocarriers, namely nanoemulsions (NE-CUR) and nanostructured lipid carriers (NLC-CUR) loaded with curcumin, and aimed to identify the most suitable nanocarrier for skin burn treatment. The study evaluated various parameters, including physicochemical characteristics, stability, encapsulation efficiency, release, skin permeation, retention, cell viability, and antimicrobial activity. The results showed that both nanocarriers showed adequate size (~200 nm), polydispersity index (~0.25), and zeta potential (~>20 mV). They also showed good encapsulation efficiency (>90%) and remained stable for 120 days at different temperatures. In the release test, NE-CUR and NCL-CUR released 57.14% and 51.64% of curcumin, respectively, in 72 h. NE-CUR demonstrated better cutaneous permeation/retention in intact or scalded skin epidermis and dermis than NLC-CUR. The cell viability test showed no toxicity after treatment with NE-CUR and NLC-CUR up to 125 g/mL. Regarding microbial activity assays, free curcumin has activity against P. aeruginosa, reducing bacterial growth by 75% in 3 h. NE-CUR inhibited bacterial growth by 65% after 24 h, and the association with gentamicin had favorable results, while NLC-CUR showed a lower inhibition. The results demonstrated that NE-CUR is probably the most promising nanocarrier for treating burns.application/pdfporToward a platform for the treatment of burns: An assessment of nanoemulsions vs. nanostructured lipid carriers loaded with curcuminAraújo, Gabriela de Moraes SoaresLoureiro, Ana Isabel SáRodrigues, Jamile LimaBarros, Paula Alice BezerraHalicki, Priscila Cristina BartolomeuRamos, Daniela FernandesMarinho, Marcelo Augusto GermaniVaiss, Daniela PastorimVaz, Gustavo RichterYurgel, Virginia CampelloBidone, JulianaMuccillo-Baisch, Ana LuizaHort, Mariana AppelCavaco-Paulo, ArturDora, Cristiana LimaHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf2227-9059DOIIsPartOf10.3390/biomedicines111233482024-05-22T10:09:20Z20232024-05-09T09:53:44Z2023-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/91451http://purl.org/coar/access_right/c_abf2open accessBurnNanotechnologyCurcuminNanoemulsionNanostructured lipid carrier3948959 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/d8f40031-9e76-4321-b55a-8f8706c57b06/download |
| spellingShingle | Toward a platform for the treatment of burns: An assessment of nanoemulsions vs. nanostructured lipid carriers loaded with curcumin Araújo, Gabriela de Moraes Soares Burn Nanotechnology Curcumin Nanoemulsion Nanostructured lipid carrier |
| status | SINGLETON |
| subject.fl_str_mv | Burn Nanotechnology Curcumin Nanoemulsion Nanostructured lipid carrier |
| title | Toward a platform for the treatment of burns: An assessment of nanoemulsions vs. nanostructured lipid carriers loaded with curcumin |
| title_full | Toward a platform for the treatment of burns: An assessment of nanoemulsions vs. nanostructured lipid carriers loaded with curcumin |
| title_fullStr | Toward a platform for the treatment of burns: An assessment of nanoemulsions vs. nanostructured lipid carriers loaded with curcumin |
| title_full_unstemmed | Toward a platform for the treatment of burns: An assessment of nanoemulsions vs. nanostructured lipid carriers loaded with curcumin |
| title_short | Toward a platform for the treatment of burns: An assessment of nanoemulsions vs. nanostructured lipid carriers loaded with curcumin |
| title_sort | Toward a platform for the treatment of burns: An assessment of nanoemulsions vs. nanostructured lipid carriers loaded with curcumin |
| topic | Burn Nanotechnology Curcumin Nanoemulsion Nanostructured lipid carrier |
| topic_facet | Burn Nanotechnology Curcumin Nanoemulsion Nanostructured lipid carrier |
| url | https://hdl.handle.net/1822/91451 |
| visible | 1 |