Publicação
Optimizing a reliable ex vivo human blood model to analyze expression of Staphylococcus epidermidis genes
| Resumo: | Human blood is often used as an ex vivo model to mimic the environment encountered by pathogens inside the host. A significant variety of experimental conditions has been reported. However, optimization strategies are often not described. This study aimed to evaluate key parameters that are expected to influence Staphylococcus epidermidis gene expression when using human blood ex vivo models. Our data confirmed that blood antimicrobial activity was dependent on initial bacterial concentration. Furthermore, blood degradation over time resulted in lower antimicrobial activity, with a 2% loss of leukocytes viability correlating with a 5-fold loss of antimicrobial activity against S. epidermidis. We further demonstrated that the volume of human blood could be reduced to as little as 0.18 mL without affecting the stability of gene expression of the tested genes. Overall, the data described herein highlight experimental parameters that should be considered when using a human blood ex vivo model for S. epidermidis gene expression analysis. |
|---|---|
| Autores principais: | Brás, Susana Maria Pereira |
| Outros Autores: | França, Ângela Maria Oliveira Sousa; Cerca, Nuno |
| Assunto: | Bacterial survival Staphylococcus epidermidis Human blood ex vivo model Volume of human blood Gene expression |
| Ano: | 2020 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867439161113313280 |
|---|---|
| author | Brás, Susana Maria Pereira |
| author2 | França, Ângela Maria Oliveira Sousa Cerca, Nuno |
| author2_role | author author |
| author_facet | Brás, Susana Maria Pereira França, Ângela Maria Oliveira Sousa Cerca, Nuno |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Brás, Susana Maria Pereira\"},{\"Person.name\":\"França, Ângela Maria Oliveira Sousa\"},{\"Person.name\":\"Cerca, Nuno\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Brás, Susana Maria Pereira França, Ângela Maria Oliveira Sousa Cerca, Nuno |
| datacite.date.Accepted.fl_str_mv | 2020-06-15T00:00:00Z |
| datacite.date.available.fl_str_mv | 2020-06-23T13:10:19Z |
| datacite.date.embargoed.fl_str_mv | 2020-06-23T13:10:19Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Bacterial survival Staphylococcus epidermidis Human blood ex vivo model Volume of human blood Gene expression |
| datacite.titles.title.fl_str_mv | Optimizing a reliable ex vivo human blood model to analyze expression of Staphylococcus epidermidis genes |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Brás, Susana Maria Pereira França, Ângela Maria Oliveira Sousa Cerca, Nuno |
| dc.date.Accepted.fl_str_mv | 2020-06-15T00:00:00Z |
| dc.date.available.fl_str_mv | 2020-06-23T13:10:19Z |
| dc.date.embargoed.fl_str_mv | 2020-06-23T13:10:19Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/65740 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Peerj, Inc. |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Bacterial survival Staphylococcus epidermidis Human blood ex vivo model Volume of human blood Gene expression |
| dc.title.fl_str_mv | Optimizing a reliable ex vivo human blood model to analyze expression of Staphylococcus epidermidis genes |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Human blood is often used as an ex vivo model to mimic the environment encountered by pathogens inside the host. A significant variety of experimental conditions has been reported. However, optimization strategies are often not described. This study aimed to evaluate key parameters that are expected to influence Staphylococcus epidermidis gene expression when using human blood ex vivo models. Our data confirmed that blood antimicrobial activity was dependent on initial bacterial concentration. Furthermore, blood degradation over time resulted in lower antimicrobial activity, with a 2% loss of leukocytes viability correlating with a 5-fold loss of antimicrobial activity against S. epidermidis. We further demonstrated that the volume of human blood could be reduced to as little as 0.18 mL without affecting the stability of gene expression of the tested genes. Overall, the data described herein highlight experimental parameters that should be considered when using a human blood ex vivo model for S. epidermidis gene expression analysis. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/5c25cd47-6d59-4d31-95fb-fcf77d16c325/download |
| id | rum_4db54ea5d4d4deaef8ddbf7278652ed0 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/65740 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/65740 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Brás, Susana Maria Pereira França, Ângela Maria Oliveira Sousa Cerca, Nuno |
| publishDate | 2020 |
| publisher.none.fl_str_mv | Peerj, Inc. |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engPeerj, Inc.porHuman blood is often used as an ex vivo model to mimic the environment encountered by pathogens inside the host. A significant variety of experimental conditions has been reported. However, optimization strategies are often not described. This study aimed to evaluate key parameters that are expected to influence Staphylococcus epidermidis gene expression when using human blood ex vivo models. Our data confirmed that blood antimicrobial activity was dependent on initial bacterial concentration. Furthermore, blood degradation over time resulted in lower antimicrobial activity, with a 2% loss of leukocytes viability correlating with a 5-fold loss of antimicrobial activity against S. epidermidis. We further demonstrated that the volume of human blood could be reduced to as little as 0.18 mL without affecting the stability of gene expression of the tested genes. Overall, the data described herein highlight experimental parameters that should be considered when using a human blood ex vivo model for S. epidermidis gene expression analysis.application/pdfporOptimizing a reliable ex vivo human blood model to analyze expression of Staphylococcus epidermidis genesBrás, Susana Maria PereiraFrança, Ângela Maria Oliveira SousaCerca, NunoHostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONBrás, Susana; França, Angela; Cerca, Nuno, Optimizing a reliable ex vivo human blood model to analyze expression of Staphylococcus epidermidis genes. PeerJ, 8(e9295), 2020ISSNIsPartOf2167-8359DOIIsPartOf10.7717/peerj.92952020-06-23T13:10:19Z2020-06-152020-06-20T09:59:28Z2020-06-15T00:00:00ZHandlehttps://hdl.handle.net/1822/65740http://purl.org/coar/access_right/c_abf2open accessBacterial survivalStaphylococcus epidermidisHuman blood ex vivo modelVolume of human bloodGene expression490806 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/5c25cd47-6d59-4d31-95fb-fcf77d16c325/download |
| spellingShingle | Optimizing a reliable ex vivo human blood model to analyze expression of Staphylococcus epidermidis genes Brás, Susana Maria Pereira Bacterial survival Staphylococcus epidermidis Human blood ex vivo model Volume of human blood Gene expression |
| status | SINGLETON |
| subject.fl_str_mv | Bacterial survival Staphylococcus epidermidis Human blood ex vivo model Volume of human blood Gene expression |
| title | Optimizing a reliable ex vivo human blood model to analyze expression of Staphylococcus epidermidis genes |
| title_full | Optimizing a reliable ex vivo human blood model to analyze expression of Staphylococcus epidermidis genes |
| title_fullStr | Optimizing a reliable ex vivo human blood model to analyze expression of Staphylococcus epidermidis genes |
| title_full_unstemmed | Optimizing a reliable ex vivo human blood model to analyze expression of Staphylococcus epidermidis genes |
| title_short | Optimizing a reliable ex vivo human blood model to analyze expression of Staphylococcus epidermidis genes |
| title_sort | Optimizing a reliable ex vivo human blood model to analyze expression of Staphylococcus epidermidis genes |
| topic | Bacterial survival Staphylococcus epidermidis Human blood ex vivo model Volume of human blood Gene expression |
| topic_facet | Bacterial survival Staphylococcus epidermidis Human blood ex vivo model Volume of human blood Gene expression |
| url | https://hdl.handle.net/1822/65740 |
| visible | 1 |