Publicação
Oxygen tension modulates cell function in an in vitro three-dimensional glioblastoma tumor model
| Resumo: | Hypoxia is a typical feature of the tumor microenvironment, one of the most critical factors affecting cell behavior and tumor progression. However, the lack of tumor models able to precisely emulate natural brain tumor tissue has impeded the study of the effects of hypoxia on the progression and growth of tumor cells. This study reports a three-dimensional (3D) brain tumor model obtained by encapsulating U87MG (U87) cells in a hydrogel containing type I collagen. It also documents the effect of various oxygen concentrations (1%, 7%, and 21%) in the culture environment on U87 cell morphology, proliferation, viability, cell cycle, apoptosis rate, and migration. Finally, it compares two-dimensional (2D) and 3D cultures. For comparison purposes, cells cultured in flat culture dishes were used as the control (2D model). Cells cultured in the 3D model proliferated more slowly but had a higher apoptosis rate and proportion of cells in the resting phase (G0 phase)/gap I phase (G1 phase) than those cultured in the 2D model. Besides, the two models yielded significantly different cell morphologies. Finally, hypoxia (e.g., 1% O2) affected cell morphology, slowed cell growth, reduced cell viability, and increased the apoptosis rate in the 3D model. These results indicate that the constructed 3D model is effective for investigating the effects of biological and chemical factors on cell morphology and function, and can be more representative of the tumor microenvironment than 2D culture systems. The developed 3D glioblastoma tumor model is equally applicable to other studies in pharmacology and pathology. |
|---|---|
| Autores principais: | Wang, Sen |
| Outros Autores: | Yao, Siqi; Pei, Na; Bai, Luge; Hao, Zhiyan; Li, Dichen; He, Jiankang; Oliveira, J. M.; Xue, Xiaoyan; Wang, Ling; Mao, Xinggang |
| Assunto: | Glioblastoma In Vitro Model Oxygen tension Glioma Hypoxia In vitro Three-dimensional glioma model |
| Ano: | 2024 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867439538917343232 |
|---|---|
| author | Wang, Sen |
| author2 | Yao, Siqi Pei, Na Bai, Luge Hao, Zhiyan Li, Dichen He, Jiankang Oliveira, J. M. Xue, Xiaoyan Wang, Ling Mao, Xinggang |
| author2_role | author author author author author author author author author author |
| author_facet | Wang, Sen Yao, Siqi Pei, Na Bai, Luge Hao, Zhiyan Li, Dichen He, Jiankang Oliveira, J. M. Xue, Xiaoyan Wang, Ling Mao, Xinggang |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Wang, Sen\"},{\"Person.name\":\"Yao, Siqi\"},{\"Person.name\":\"Pei, Na\"},{\"Person.name\":\"Bai, Luge\"},{\"Person.name\":\"Hao, Zhiyan\"},{\"Person.name\":\"Li, Dichen\"},{\"Person.name\":\"He, Jiankang\"},{\"Person.name\":\"Oliveira, J. M.\"},{\"Person.name\":\"Xue, Xiaoyan\"},{\"Person.name\":\"Wang, Ling\"},{\"Person.name\":\"Mao, Xinggang\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Wang, Sen Yao, Siqi Pei, Na Bai, Luge Hao, Zhiyan Li, Dichen He, Jiankang Oliveira, J. M. Xue, Xiaoyan Wang, Ling Mao, Xinggang |
| datacite.date.Accepted.fl_str_mv | 2024-04-01T00:00:00Z |
| datacite.date.embargoed.fl_str_mv | 10000-01-01T00:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Glioblastoma In Vitro Model Oxygen tension Glioma Hypoxia In vitro Three-dimensional glioma model |
| datacite.titles.title.fl_str_mv | Oxygen tension modulates cell function in an in vitro three-dimensional glioblastoma tumor model |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Wang, Sen Yao, Siqi Pei, Na Bai, Luge Hao, Zhiyan Li, Dichen He, Jiankang Oliveira, J. M. Xue, Xiaoyan Wang, Ling Mao, Xinggang |
| dc.date.Accepted.fl_str_mv | 2024-04-01T00:00:00Z |
| dc.date.embargoed.fl_str_mv | 10000-01-01T00:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/91087 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Springer |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Glioblastoma In Vitro Model Oxygen tension Glioma Hypoxia In vitro Three-dimensional glioma model |
| dc.title.fl_str_mv | Oxygen tension modulates cell function in an in vitro three-dimensional glioblastoma tumor model |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Hypoxia is a typical feature of the tumor microenvironment, one of the most critical factors affecting cell behavior and tumor progression. However, the lack of tumor models able to precisely emulate natural brain tumor tissue has impeded the study of the effects of hypoxia on the progression and growth of tumor cells. This study reports a three-dimensional (3D) brain tumor model obtained by encapsulating U87MG (U87) cells in a hydrogel containing type I collagen. It also documents the effect of various oxygen concentrations (1%, 7%, and 21%) in the culture environment on U87 cell morphology, proliferation, viability, cell cycle, apoptosis rate, and migration. Finally, it compares two-dimensional (2D) and 3D cultures. For comparison purposes, cells cultured in flat culture dishes were used as the control (2D model). Cells cultured in the 3D model proliferated more slowly but had a higher apoptosis rate and proportion of cells in the resting phase (G0 phase)/gap I phase (G1 phase) than those cultured in the 2D model. Besides, the two models yielded significantly different cell morphologies. Finally, hypoxia (e.g., 1% O2) affected cell morphology, slowed cell growth, reduced cell viability, and increased the apoptosis rate in the 3D model. These results indicate that the constructed 3D model is effective for investigating the effects of biological and chemical factors on cell morphology and function, and can be more representative of the tumor microenvironment than 2D culture systems. The developed 3D glioblastoma tumor model is equally applicable to other studies in pharmacology and pathology. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/81710994-0079-467e-992f-bb397101e9d1/download |
| id | rum_4df247e9c60447767b3a07f00aaf2fd1 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/91087 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/91087 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Wang, Sen Yao, Siqi Pei, Na Bai, Luge Hao, Zhiyan Li, Dichen He, Jiankang Oliveira, J. M. Xue, Xiaoyan Wang, Ling Mao, Xinggang |
| publishDate | 2024 |
| publisher.none.fl_str_mv | Springer |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engSpringerporHypoxia is a typical feature of the tumor microenvironment, one of the most critical factors affecting cell behavior and tumor progression. However, the lack of tumor models able to precisely emulate natural brain tumor tissue has impeded the study of the effects of hypoxia on the progression and growth of tumor cells. This study reports a three-dimensional (3D) brain tumor model obtained by encapsulating U87MG (U87) cells in a hydrogel containing type I collagen. It also documents the effect of various oxygen concentrations (1%, 7%, and 21%) in the culture environment on U87 cell morphology, proliferation, viability, cell cycle, apoptosis rate, and migration. Finally, it compares two-dimensional (2D) and 3D cultures. For comparison purposes, cells cultured in flat culture dishes were used as the control (2D model). Cells cultured in the 3D model proliferated more slowly but had a higher apoptosis rate and proportion of cells in the resting phase (G0 phase)/gap I phase (G1 phase) than those cultured in the 2D model. Besides, the two models yielded significantly different cell morphologies. Finally, hypoxia (e.g., 1% O2) affected cell morphology, slowed cell growth, reduced cell viability, and increased the apoptosis rate in the 3D model. These results indicate that the constructed 3D model is effective for investigating the effects of biological and chemical factors on cell morphology and function, and can be more representative of the tumor microenvironment than 2D culture systems. The developed 3D glioblastoma tumor model is equally applicable to other studies in pharmacology and pathology.application/pdfporOxygen tension modulates cell function in an in vitro three-dimensional glioblastoma tumor modelWang, SenYao, SiqiPei, NaBai, LugeHao, ZhiyanLi, DichenHe, JiankangOliveira, J. M.Xue, XiaoyanWang, LingMao, XinggangHostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONWang S., Yao S., Pei N., Bai L., Hao Z., Li D., He J., Oliveira J. M., Xue X., Wang L., Mao X. Oxygen tension modulates cell function in an in vitro three-dimensional glioblastoma tumor model, Bio-Design and Manufacturing, pp. 1-13, doi:0.1007/s42242-024-00271-9, 2024ISSNIsPartOf2096-5524EISSNIsPartOf2522-8552DOIIsPartOf10.1007/s42242-024-00271-92024-042024-042024-04-17T15:17:12Z2024-04-01T00:00:00Z10000-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/91087http://purl.org/coar/access_right/c_16ecrestricted accessGlioblastomaIn Vitro ModelOxygen tensionGliomaHypoxiaIn vitroThree-dimensional glioma model3410124 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_f1cfapplication/pdffulltexthttps://repositorium.uminho.pt/bitstreams/81710994-0079-467e-992f-bb397101e9d1/download |
| spellingShingle | Oxygen tension modulates cell function in an in vitro three-dimensional glioblastoma tumor model Wang, Sen Glioblastoma In Vitro Model Oxygen tension Glioma Hypoxia In vitro Three-dimensional glioma model |
| status | SINGLETON |
| subject.fl_str_mv | Glioblastoma In Vitro Model Oxygen tension Glioma Hypoxia In vitro Three-dimensional glioma model |
| title | Oxygen tension modulates cell function in an in vitro three-dimensional glioblastoma tumor model |
| title_full | Oxygen tension modulates cell function in an in vitro three-dimensional glioblastoma tumor model |
| title_fullStr | Oxygen tension modulates cell function in an in vitro three-dimensional glioblastoma tumor model |
| title_full_unstemmed | Oxygen tension modulates cell function in an in vitro three-dimensional glioblastoma tumor model |
| title_short | Oxygen tension modulates cell function in an in vitro three-dimensional glioblastoma tumor model |
| title_sort | Oxygen tension modulates cell function in an in vitro three-dimensional glioblastoma tumor model |
| topic | Glioblastoma In Vitro Model Oxygen tension Glioma Hypoxia In vitro Three-dimensional glioma model |
| topic_facet | Glioblastoma In Vitro Model Oxygen tension Glioma Hypoxia In vitro Three-dimensional glioma model |
| url | https://hdl.handle.net/1822/91087 |
| visible | 1 |