Publicação
A biologically active delivery material with dried-rehydrated vesicles containing the anti-inflammatory diclofenac for potential wound healing
| Resumo: | AbstractChronic wounds usually remain in the inflammatory phase of the healing process during several months or even years. Hence, a continuous research has been resulting in the development of wound dressings with improved performance. Herein, we report a delivery system for cutaneous wound healing, consisting of a textile material (non-woven gauzes) covered with lipidic vesicles containing diclofenac, a non-steroidal anti-inflammatory drug (NSAID). This study also aims to compare the entrapment efficiency data with previous works and confirm that this parameter and drug amount are not directly correlated. A method of dehydrationrehydration of the liposomes presenting different sizes and lamellarities was used to assess the best conditions to attain the highest drug entrapment efficiency. Optimum conditions for the NSAID release were achieved with high phospholipid concentrations and dried-rehydrated vesicles (DRVs) prepared from multilamellar liposomes (MLVs). A chemical activation of the gauzes was performed to enhance the vesicles attachment, also contributing to a higher drug amount in the surrounding media. In spite of the entrapment efficiency being lower comparatively with other values presented by us previously, the diclofenac concentration was considerably higher in this formulation. Entrapment efficiency is, therefore, not sufficient per se to define the real amount of drug contained in the formulation. The cytocompatibility assessment in human skin fibroblasts showed that DRVs from MLVs and DRVs from large unilamellar liposomes (LUVs) with less than 750??M of egg-yolk phosphatidylcholine (EPC), containing diclofenac, were not cytotoxic after 72?h of contact, greatly implying potential for their application in the chronic wounds healing. |
|---|---|
| Autores principais: | Ferreira, Helena |
| Outros Autores: | Silva, Raquel; Matamá, Maria Teresa; Silva, Carla; Gomes, Andreia C; Cavaco-Paulo, Artur |
| Assunto: | Cationized gauzes Effective concentration Entrapment efficiency Non-steroidal anti-inflammatory drug Wound healing |
| Ano: | 2016 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866875799133487104 |
|---|---|
| author | Ferreira, Helena |
| author2 | Silva, Raquel Matamá, Maria Teresa Silva, Carla Gomes, Andreia C Cavaco-Paulo, Artur |
| author2_role | author author author author author |
| author_facet | Ferreira, Helena Silva, Raquel Matamá, Maria Teresa Silva, Carla Gomes, Andreia C Cavaco-Paulo, Artur |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Ferreira, Helena\"},{\"Person.name\":\"Silva, Raquel\"},{\"Person.name\":\"Matamá, Maria Teresa\"},{\"Person.name\":\"Silva, Carla\"},{\"Person.name\":\"Gomes, Andreia C\"},{\"Person.name\":\"Cavaco-Paulo, Artur\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Ferreira, Helena Silva, Raquel Matamá, Maria Teresa Silva, Carla Gomes, Andreia C Cavaco-Paulo, Artur |
| datacite.date.Accepted.fl_str_mv | 2016-01-01T00:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Cationized gauzes Effective concentration Entrapment efficiency Non-steroidal anti-inflammatory drug Wound healing |
| datacite.titles.title.fl_str_mv | A biologically active delivery material with dried-rehydrated vesicles containing the anti-inflammatory diclofenac for potential wound healing |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Ferreira, Helena Silva, Raquel Matamá, Maria Teresa Silva, Carla Gomes, Andreia C Cavaco-Paulo, Artur |
| dc.date.Accepted.fl_str_mv | 2016-01-01T00:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/44744 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Taylor and Francis |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Cationized gauzes Effective concentration Entrapment efficiency Non-steroidal anti-inflammatory drug Wound healing |
| dc.title.fl_str_mv | A biologically active delivery material with dried-rehydrated vesicles containing the anti-inflammatory diclofenac for potential wound healing |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | AbstractChronic wounds usually remain in the inflammatory phase of the healing process during several months or even years. Hence, a continuous research has been resulting in the development of wound dressings with improved performance. Herein, we report a delivery system for cutaneous wound healing, consisting of a textile material (non-woven gauzes) covered with lipidic vesicles containing diclofenac, a non-steroidal anti-inflammatory drug (NSAID). This study also aims to compare the entrapment efficiency data with previous works and confirm that this parameter and drug amount are not directly correlated. A method of dehydrationrehydration of the liposomes presenting different sizes and lamellarities was used to assess the best conditions to attain the highest drug entrapment efficiency. Optimum conditions for the NSAID release were achieved with high phospholipid concentrations and dried-rehydrated vesicles (DRVs) prepared from multilamellar liposomes (MLVs). A chemical activation of the gauzes was performed to enhance the vesicles attachment, also contributing to a higher drug amount in the surrounding media. In spite of the entrapment efficiency being lower comparatively with other values presented by us previously, the diclofenac concentration was considerably higher in this formulation. Entrapment efficiency is, therefore, not sufficient per se to define the real amount of drug contained in the formulation. The cytocompatibility assessment in human skin fibroblasts showed that DRVs from MLVs and DRVs from large unilamellar liposomes (LUVs) with less than 750??M of egg-yolk phosphatidylcholine (EPC), containing diclofenac, were not cytotoxic after 72?h of contact, greatly implying potential for their application in the chronic wounds healing. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/10e340c9-6a1c-42e1-aa05-40217017566d/download |
| id | rum_57f0dc615e4e041112ee00a6f4e8b871 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/44744 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/44744 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Ferreira, Helena Silva, Raquel Matamá, Maria Teresa Silva, Carla Gomes, Andreia C Cavaco-Paulo, Artur |
| publishDate | 2016 |
| publisher.none.fl_str_mv | Taylor and Francis |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engTaylor and FrancisporAbstractChronic wounds usually remain in the inflammatory phase of the healing process during several months or even years. Hence, a continuous research has been resulting in the development of wound dressings with improved performance. Herein, we report a delivery system for cutaneous wound healing, consisting of a textile material (non-woven gauzes) covered with lipidic vesicles containing diclofenac, a non-steroidal anti-inflammatory drug (NSAID). This study also aims to compare the entrapment efficiency data with previous works and confirm that this parameter and drug amount are not directly correlated. A method of dehydrationrehydration of the liposomes presenting different sizes and lamellarities was used to assess the best conditions to attain the highest drug entrapment efficiency. Optimum conditions for the NSAID release were achieved with high phospholipid concentrations and dried-rehydrated vesicles (DRVs) prepared from multilamellar liposomes (MLVs). A chemical activation of the gauzes was performed to enhance the vesicles attachment, also contributing to a higher drug amount in the surrounding media. In spite of the entrapment efficiency being lower comparatively with other values presented by us previously, the diclofenac concentration was considerably higher in this formulation. Entrapment efficiency is, therefore, not sufficient per se to define the real amount of drug contained in the formulation. The cytocompatibility assessment in human skin fibroblasts showed that DRVs from MLVs and DRVs from large unilamellar liposomes (LUVs) with less than 750??M of egg-yolk phosphatidylcholine (EPC), containing diclofenac, were not cytotoxic after 72?h of contact, greatly implying potential for their application in the chronic wounds healing.application/pdfporA biologically active delivery material with dried-rehydrated vesicles containing the anti-inflammatory diclofenac for potential wound healingFerreira, HelenaSilva, RaquelMatamá, Maria TeresaSilva, CarlaGomes, Andreia CCavaco-Paulo, ArturHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf0898-2104DOIIsPartOf10.3109/08982104.2015.110833320162017-02-04T17:18:09Z2016-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/44744http://purl.org/coar/access_right/c_16ecrestricted accessCationized gauzesEffective concentrationEntrapment efficiencyNon-steroidal anti-inflammatory drugWound healing418234 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/10e340c9-6a1c-42e1-aa05-40217017566d/download |
| spellingShingle | A biologically active delivery material with dried-rehydrated vesicles containing the anti-inflammatory diclofenac for potential wound healing Ferreira, Helena Cationized gauzes Effective concentration Entrapment efficiency Non-steroidal anti-inflammatory drug Wound healing |
| status | SINGLETON |
| subject.fl_str_mv | Cationized gauzes Effective concentration Entrapment efficiency Non-steroidal anti-inflammatory drug Wound healing |
| title | A biologically active delivery material with dried-rehydrated vesicles containing the anti-inflammatory diclofenac for potential wound healing |
| title_full | A biologically active delivery material with dried-rehydrated vesicles containing the anti-inflammatory diclofenac for potential wound healing |
| title_fullStr | A biologically active delivery material with dried-rehydrated vesicles containing the anti-inflammatory diclofenac for potential wound healing |
| title_full_unstemmed | A biologically active delivery material with dried-rehydrated vesicles containing the anti-inflammatory diclofenac for potential wound healing |
| title_short | A biologically active delivery material with dried-rehydrated vesicles containing the anti-inflammatory diclofenac for potential wound healing |
| title_sort | A biologically active delivery material with dried-rehydrated vesicles containing the anti-inflammatory diclofenac for potential wound healing |
| topic | Cationized gauzes Effective concentration Entrapment efficiency Non-steroidal anti-inflammatory drug Wound healing |
| topic_facet | Cationized gauzes Effective concentration Entrapment efficiency Non-steroidal anti-inflammatory drug Wound healing |
| url | https://hdl.handle.net/1822/44744 |
| visible | 1 |