Publicação
Characterizing HIV-1 genetic subtypes and drug resistance mutations among children, adolescents and pregnant women in Sierra Leone
| Resumo: | Human immunodeficiency virus (HIV) drug resistance (HIVDR) is widespread in sub-Saharan Africa. Children and pregnant women are particularly vulnerable, and laboratory testing capacity remains limited. We, therefore, used a cross-sectional design and convenience sampling to characterize HIV subtypes and resistance-associated mutations (RAMs) in these groups in Sierra Leone. In total, 96 children (age 2–9 years, 100% ART-experienced), 47 adolescents (age 10–18 years, 100% ART-experienced), and 54 pregnant women (>18 years, 72% ART-experienced) were enrolled. Median treatment durations were 36, 84, and 3 months, respectively, while the sequencing success rates were 45%, 70%, and 59%, respectively, among children, adolescents, and pregnant women. Overall, the predominant HIV-1 subtype was CRF02_AG (87.9%, 95/108), with minority variants constituting 12%. Among children and adolescents, the most common RAMs were M184V (76.6%, <i>n</i> = 49/64), K103N (45.3%, <i>n</i> = 29/64), Y181C/V/I (28.1%, <i>n</i> = 18/64), T215F/Y (25.0%, <i>n</i> = 16/64), and V108I (18.8%, <i>n</i> = 12/64). Among pregnant women, the most frequent RAMs were K103N (20.6%, <i>n</i> = 7/34), M184V (11.8%, <i>n</i> = 4/34), Y181C/V/I (5.9%, <i>n</i> = 2/34), P225H (8.8%, <i>n</i> = 3/34), and K219N/E/Q/R (5.9%, <i>n</i> = 2/34). Protease and integrase inhibitor-RAMs were relatively few or absent. Based on the genotype susceptibility score distributions, 73%, 88%, and 14% of children, adolescents, and pregnant women, respectively, were not susceptible to all three drug components of the WHO preferred first-line regimens per 2018 guidelines. These findings suggest that routine HIVDR surveillance and access to better ART choices may improve treatment outcomes in Sierra Leone. |
|---|---|
| Autores principais: | Yendewa, George A. |
| Outros Autores: | Lakoh, Sulaiman; Yendewa, Sahr A.; Bangura, Khadijah; Tabernilla, Andrés; Patiño, Lucia; Jiba, Darlinda F.; Vandy, Alren O.; Massaquoi, Samuel P.; Osório, Nuno S.; Deen, Gibrilla F.; Sahr, Foday; Salata, Robert A.; Poveda, Eva |
| Assunto: | HIV Drug resistance Children Adolescents Pregnant women Resource-limited settings Sierra Leone |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866877812998144000 |
|---|---|
| author | Yendewa, George A. |
| author2 | Lakoh, Sulaiman Yendewa, Sahr A. Bangura, Khadijah Tabernilla, Andrés Patiño, Lucia Jiba, Darlinda F. Vandy, Alren O. Massaquoi, Samuel P. Osório, Nuno S. Deen, Gibrilla F. Sahr, Foday Salata, Robert A. Poveda, Eva |
| author2_role | author author author author author author author author author author author author author |
| author_facet | Yendewa, George A. Lakoh, Sulaiman Yendewa, Sahr A. Bangura, Khadijah Tabernilla, Andrés Patiño, Lucia Jiba, Darlinda F. Vandy, Alren O. Massaquoi, Samuel P. Osório, Nuno S. Deen, Gibrilla F. Sahr, Foday Salata, Robert A. Poveda, Eva |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Yendewa, George A.\"},{\"Person.name\":\"Lakoh, Sulaiman\"},{\"Person.name\":\"Yendewa, Sahr A.\"},{\"Person.name\":\"Bangura, Khadijah\"},{\"Person.name\":\"Tabernilla, Andrés\"},{\"Person.name\":\"Patiño, Lucia\"},{\"Person.name\":\"Jiba, Darlinda F.\"},{\"Person.name\":\"Vandy, Alren O.\"},{\"Person.name\":\"Massaquoi, Samuel P.\"},{\"Person.name\":\"Osório, Nuno S.\"},{\"Person.name\":\"Deen, Gibrilla F.\"},{\"Person.name\":\"Sahr, Foday\"},{\"Person.name\":\"Salata, Robert A.\"},{\"Person.name\":\"Poveda, Eva\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Yendewa, George A. Lakoh, Sulaiman Yendewa, Sahr A. Bangura, Khadijah Tabernilla, Andrés Patiño, Lucia Jiba, Darlinda F. Vandy, Alren O. Massaquoi, Samuel P. Osório, Nuno S. Deen, Gibrilla F. Sahr, Foday Salata, Robert A. Poveda, Eva |
| datacite.date.Accepted.fl_str_mv | 2021-08-26T00:00:00Z |
| datacite.date.available.fl_str_mv | 2021-11-26T10:34:47Z |
| datacite.date.embargoed.fl_str_mv | 2021-11-26T10:34:47Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | HIV Drug resistance Children Adolescents Pregnant women Resource-limited settings Sierra Leone |
| datacite.titles.title.fl_str_mv | Characterizing HIV-1 genetic subtypes and drug resistance mutations among children, adolescents and pregnant women in Sierra Leone |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Yendewa, George A. Lakoh, Sulaiman Yendewa, Sahr A. Bangura, Khadijah Tabernilla, Andrés Patiño, Lucia Jiba, Darlinda F. Vandy, Alren O. Massaquoi, Samuel P. Osório, Nuno S. Deen, Gibrilla F. Sahr, Foday Salata, Robert A. Poveda, Eva |
| dc.date.Accepted.fl_str_mv | 2021-08-26T00:00:00Z |
| dc.date.available.fl_str_mv | 2021-11-26T10:34:47Z |
| dc.date.embargoed.fl_str_mv | 2021-11-26T10:34:47Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/74785 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Multidisciplinary Digital Publishing Institute (MDPI) |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.rights.rights.copyright.fl_str_mv | openAccess |
| dc.subject.none.fl_str_mv | HIV Drug resistance Children Adolescents Pregnant women Resource-limited settings Sierra Leone |
| dc.title.fl_str_mv | Characterizing HIV-1 genetic subtypes and drug resistance mutations among children, adolescents and pregnant women in Sierra Leone |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Human immunodeficiency virus (HIV) drug resistance (HIVDR) is widespread in sub-Saharan Africa. Children and pregnant women are particularly vulnerable, and laboratory testing capacity remains limited. We, therefore, used a cross-sectional design and convenience sampling to characterize HIV subtypes and resistance-associated mutations (RAMs) in these groups in Sierra Leone. In total, 96 children (age 2–9 years, 100% ART-experienced), 47 adolescents (age 10–18 years, 100% ART-experienced), and 54 pregnant women (>18 years, 72% ART-experienced) were enrolled. Median treatment durations were 36, 84, and 3 months, respectively, while the sequencing success rates were 45%, 70%, and 59%, respectively, among children, adolescents, and pregnant women. Overall, the predominant HIV-1 subtype was CRF02_AG (87.9%, 95/108), with minority variants constituting 12%. Among children and adolescents, the most common RAMs were M184V (76.6%, <i>n</i> = 49/64), K103N (45.3%, <i>n</i> = 29/64), Y181C/V/I (28.1%, <i>n</i> = 18/64), T215F/Y (25.0%, <i>n</i> = 16/64), and V108I (18.8%, <i>n</i> = 12/64). Among pregnant women, the most frequent RAMs were K103N (20.6%, <i>n</i> = 7/34), M184V (11.8%, <i>n</i> = 4/34), Y181C/V/I (5.9%, <i>n</i> = 2/34), P225H (8.8%, <i>n</i> = 3/34), and K219N/E/Q/R (5.9%, <i>n</i> = 2/34). Protease and integrase inhibitor-RAMs were relatively few or absent. Based on the genotype susceptibility score distributions, 73%, 88%, and 14% of children, adolescents, and pregnant women, respectively, were not susceptible to all three drug components of the WHO preferred first-line regimens per 2018 guidelines. These findings suggest that routine HIVDR surveillance and access to better ART choices may improve treatment outcomes in Sierra Leone. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/19e86885-1b8c-4108-9ff7-e7ce5017f3e6/download |
| id | rum_5865e1793ffd4e06332d8ee73de68aea |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/74785 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/74785 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Yendewa, George A. Lakoh, Sulaiman Yendewa, Sahr A. Bangura, Khadijah Tabernilla, Andrés Patiño, Lucia Jiba, Darlinda F. Vandy, Alren O. Massaquoi, Samuel P. Osório, Nuno S. Deen, Gibrilla F. Sahr, Foday Salata, Robert A. Poveda, Eva |
| publishDate | 2021 |
| publisher.none.fl_str_mv | Multidisciplinary Digital Publishing Institute (MDPI) |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engMultidisciplinary Digital Publishing Institute (MDPI)porHuman immunodeficiency virus (HIV) drug resistance (HIVDR) is widespread in sub-Saharan Africa. Children and pregnant women are particularly vulnerable, and laboratory testing capacity remains limited. We, therefore, used a cross-sectional design and convenience sampling to characterize HIV subtypes and resistance-associated mutations (RAMs) in these groups in Sierra Leone. In total, 96 children (age 2–9 years, 100% ART-experienced), 47 adolescents (age 10–18 years, 100% ART-experienced), and 54 pregnant women (>18 years, 72% ART-experienced) were enrolled. Median treatment durations were 36, 84, and 3 months, respectively, while the sequencing success rates were 45%, 70%, and 59%, respectively, among children, adolescents, and pregnant women. Overall, the predominant HIV-1 subtype was CRF02_AG (87.9%, 95/108), with minority variants constituting 12%. Among children and adolescents, the most common RAMs were M184V (76.6%, <i>n</i> = 49/64), K103N (45.3%, <i>n</i> = 29/64), Y181C/V/I (28.1%, <i>n</i> = 18/64), T215F/Y (25.0%, <i>n</i> = 16/64), and V108I (18.8%, <i>n</i> = 12/64). Among pregnant women, the most frequent RAMs were K103N (20.6%, <i>n</i> = 7/34), M184V (11.8%, <i>n</i> = 4/34), Y181C/V/I (5.9%, <i>n</i> = 2/34), P225H (8.8%, <i>n</i> = 3/34), and K219N/E/Q/R (5.9%, <i>n</i> = 2/34). Protease and integrase inhibitor-RAMs were relatively few or absent. Based on the genotype susceptibility score distributions, 73%, 88%, and 14% of children, adolescents, and pregnant women, respectively, were not susceptible to all three drug components of the WHO preferred first-line regimens per 2018 guidelines. These findings suggest that routine HIVDR surveillance and access to better ART choices may improve treatment outcomes in Sierra Leone.application/pdfporCharacterizing HIV-1 genetic subtypes and drug resistance mutations among children, adolescents and pregnant women in Sierra LeoneYendewa, George A.Lakoh, SulaimanYendewa, Sahr A.Bangura, KhadijahTabernilla, AndrésPatiño, LuciaJiba, Darlinda F.Vandy, Alren O.Massaquoi, Samuel P.Osório, Nuno S.Deen, Gibrilla F.Sahr, FodaySalata, Robert A.Poveda, EvaHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptDOIIsPartOf10.3390/genes120913142021-11-26T10:34:47Z2021-08-262021-09-25T23:33:54Z2021-08-26T00:00:00ZHandlehttps://hdl.handle.net/1822/74785http://purl.org/coar/access_right/c_abf2open accessHIVDrug resistanceChildrenAdolescentsPregnant womenResource-limited settingsSierra Leone503928 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2021-08-26http://creativecommons.org/licenses/by/4.0/openAccesshttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/19e86885-1b8c-4108-9ff7-e7ce5017f3e6/download |
| spellingShingle | Characterizing HIV-1 genetic subtypes and drug resistance mutations among children, adolescents and pregnant women in Sierra Leone Yendewa, George A. HIV Drug resistance Children Adolescents Pregnant women Resource-limited settings Sierra Leone |
| status | SINGLETON |
| subject.fl_str_mv | HIV Drug resistance Children Adolescents Pregnant women Resource-limited settings Sierra Leone |
| title | Characterizing HIV-1 genetic subtypes and drug resistance mutations among children, adolescents and pregnant women in Sierra Leone |
| title_full | Characterizing HIV-1 genetic subtypes and drug resistance mutations among children, adolescents and pregnant women in Sierra Leone |
| title_fullStr | Characterizing HIV-1 genetic subtypes and drug resistance mutations among children, adolescents and pregnant women in Sierra Leone |
| title_full_unstemmed | Characterizing HIV-1 genetic subtypes and drug resistance mutations among children, adolescents and pregnant women in Sierra Leone |
| title_short | Characterizing HIV-1 genetic subtypes and drug resistance mutations among children, adolescents and pregnant women in Sierra Leone |
| title_sort | Characterizing HIV-1 genetic subtypes and drug resistance mutations among children, adolescents and pregnant women in Sierra Leone |
| topic | HIV Drug resistance Children Adolescents Pregnant women Resource-limited settings Sierra Leone |
| topic_facet | HIV Drug resistance Children Adolescents Pregnant women Resource-limited settings Sierra Leone |
| url | https://hdl.handle.net/1822/74785 |
| visible | 1 |