Publicação
Characterisation of chilean hazelnut (gevuina avellana) tissues : light microscopy and cell wall polysaccharides
| Resumo: | By applying several differential staining techniques and light microscopy, the structure and composition of Chilean hazelnut (Gevuina avellana) seeds were analysed. The structure of the G avellana seed is very simple, with a thin, heavily lignified seed coat and two voluminous cotyledons. The embryo food reserves are uniformly distributed over the cotyledon cells. The cell wall polysaccharides were recovered from the alcohol-insoluble residue by mild treatment with warm chlorite solution and sequential extraction with alkali solutions of increasing concentration. FT-IR spectra in the 1200–850 cm-1 region were used together with chemometric techniques to distinguish the hemicellulosic and pectic polysaccharides in the extracts. The most abundant extracts were fractionated by graded precipitation in ethanol. A xyloglucan was identified by 1H and 13C NMR as the major hemicellulosic polysaccharide, with a sugar composition of 4Glc:3.5Xyl:1Gal:0.5Fuc. The hazelnut cell walls are composed of equivalent amounts of pectic polysaccharides, xyloglucans and cellulose. |
|---|---|
| Autores principais: | Dourado, Fernando |
| Outros Autores: | Vasco, Pedro; Barros, António; Mota, M.; Coimbra, Manuel A.; Gama, F. M. |
| Assunto: | Hazelnut Gevuina avellana Seed structure Cell walls Light microscopy FT-IR Xyloglucan |
| Ano: | 2003 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1864251907956015104 |
|---|---|
| author | Dourado, Fernando |
| author2 | Vasco, Pedro Barros, António Mota, M. Coimbra, Manuel A. Gama, F. M. |
| author2_role | author author author author author |
| author_facet | Dourado, Fernando Vasco, Pedro Barros, António Mota, M. Coimbra, Manuel A. Gama, F. M. |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Dourado, Fernando\"},{\"Person.name\":\"Vasco, Pedro\"},{\"Person.name\":\"Barros, António\"},{\"Person.name\":\"Mota, M.\"},{\"Person.name\":\"Coimbra, Manuel A.\"},{\"Person.name\":\"Gama, F. M.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Dourado, Fernando Vasco, Pedro Barros, António Mota, M. Coimbra, Manuel A. Gama, F. M. |
| datacite.date.Accepted.fl_str_mv | 2003-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2005-05-03T12:38:19Z |
| datacite.date.embargoed.fl_str_mv | 2005-05-03T12:38:19Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Hazelnut Gevuina avellana Seed structure Cell walls Light microscopy FT-IR Xyloglucan |
| datacite.titles.title.fl_str_mv | Characterisation of chilean hazelnut (gevuina avellana) tissues : light microscopy and cell wall polysaccharides |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Dourado, Fernando Vasco, Pedro Barros, António Mota, M. Coimbra, Manuel A. Gama, F. M. |
| dc.date.Accepted.fl_str_mv | 2003-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2005-05-03T12:38:19Z |
| dc.date.embargoed.fl_str_mv | 2005-05-03T12:38:19Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/1387 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Society of Chemical Industry |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Hazelnut Gevuina avellana Seed structure Cell walls Light microscopy FT-IR Xyloglucan |
| dc.title.fl_str_mv | Characterisation of chilean hazelnut (gevuina avellana) tissues : light microscopy and cell wall polysaccharides |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | By applying several differential staining techniques and light microscopy, the structure and composition of Chilean hazelnut (Gevuina avellana) seeds were analysed. The structure of the G avellana seed is very simple, with a thin, heavily lignified seed coat and two voluminous cotyledons. The embryo food reserves are uniformly distributed over the cotyledon cells. The cell wall polysaccharides were recovered from the alcohol-insoluble residue by mild treatment with warm chlorite solution and sequential extraction with alkali solutions of increasing concentration. FT-IR spectra in the 1200–850 cm-1 region were used together with chemometric techniques to distinguish the hemicellulosic and pectic polysaccharides in the extracts. The most abundant extracts were fractionated by graded precipitation in ethanol. A xyloglucan was identified by 1H and 13C NMR as the major hemicellulosic polysaccharide, with a sugar composition of 4Glc:3.5Xyl:1Gal:0.5Fuc. The hazelnut cell walls are composed of equivalent amounts of pectic polysaccharides, xyloglucans and cellulose. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/e1980520-8ec1-48f6-bb21-66108b358fab/download |
| id | rum_5af7ce00a68ae070bcefac3b29201c95 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/1387 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/1387 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Dourado, Fernando Vasco, Pedro Barros, António Mota, M. Coimbra, Manuel A. Gama, F. M. |
| publishDate | 2003 |
| publisher.none.fl_str_mv | Society of Chemical Industry |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engSociety of Chemical IndustryengBy applying several differential staining techniques and light microscopy, the structure and composition of Chilean hazelnut (Gevuina avellana) seeds were analysed. The structure of the G avellana seed is very simple, with a thin, heavily lignified seed coat and two voluminous cotyledons. The embryo food reserves are uniformly distributed over the cotyledon cells. The cell wall polysaccharides were recovered from the alcohol-insoluble residue by mild treatment with warm chlorite solution and sequential extraction with alkali solutions of increasing concentration. FT-IR spectra in the 1200–850 cm-1 region were used together with chemometric techniques to distinguish the hemicellulosic and pectic polysaccharides in the extracts. The most abundant extracts were fractionated by graded precipitation in ethanol. A xyloglucan was identified by 1H and 13C NMR as the major hemicellulosic polysaccharide, with a sugar composition of 4Glc:3.5Xyl:1Gal:0.5Fuc. The hazelnut cell walls are composed of equivalent amounts of pectic polysaccharides, xyloglucans and cellulose.application/pdfengCharacterisation of chilean hazelnut (gevuina avellana) tissues : light microscopy and cell wall polysaccharidesDourado, FernandoVasco, PedroBarros, AntónioMota, M.Coimbra, Manuel A.Gama, F. M.HostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf0022-5142DOIIsPartOf10.1002/jsfa.12872005-05-03T12:38:19Z20032003-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/1387http://purl.org/coar/access_right/c_abf2open accessHazelnutGevuina avellanaSeed structureCell wallsLight microscopyFT-IRXyloglucan427664 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/e1980520-8ec1-48f6-bb21-66108b358fab/download |
| spellingShingle | Characterisation of chilean hazelnut (gevuina avellana) tissues : light microscopy and cell wall polysaccharides Dourado, Fernando Hazelnut Gevuina avellana Seed structure Cell walls Light microscopy FT-IR Xyloglucan |
| subject.fl_str_mv | Hazelnut Gevuina avellana Seed structure Cell walls Light microscopy FT-IR Xyloglucan |
| title | Characterisation of chilean hazelnut (gevuina avellana) tissues : light microscopy and cell wall polysaccharides |
| title_full | Characterisation of chilean hazelnut (gevuina avellana) tissues : light microscopy and cell wall polysaccharides |
| title_fullStr | Characterisation of chilean hazelnut (gevuina avellana) tissues : light microscopy and cell wall polysaccharides |
| title_full_unstemmed | Characterisation of chilean hazelnut (gevuina avellana) tissues : light microscopy and cell wall polysaccharides |
| title_short | Characterisation of chilean hazelnut (gevuina avellana) tissues : light microscopy and cell wall polysaccharides |
| title_sort | Characterisation of chilean hazelnut (gevuina avellana) tissues : light microscopy and cell wall polysaccharides |
| topic | Hazelnut Gevuina avellana Seed structure Cell walls Light microscopy FT-IR Xyloglucan |
| topic_facet | Hazelnut Gevuina avellana Seed structure Cell walls Light microscopy FT-IR Xyloglucan |
| url | https://hdl.handle.net/1822/1387 |
| visible | 1 |