Publicação
Detection of BCG bacteria using a magnetoresistive biosensor: A step towards a fully electronic platform for tuberculosis point-of-care detection
| Resumo: | Tuberculosis is one of the major public health concerns. This highly contagious disease affects more than 10.4 million people, being a leading cause of morbidity by infection. Tuberculosis is diagnosed at the point-of-care by the Ziehl-Neelsen sputum smear microscopy test. Ziehl-Neelsen is laborious, prone to human error and infection risk, with a limit of detection of 104 cells/mL. In resource-poor nations, a more practical test, with lower detection limit, is paramount. This work uses a magnetoresistive biosensor to detect BCG bacteria for tuberculosis diagnosis. Herein we report: i) nanoparticle assembly method and specificity for tuberculosis detection; ii) demonstration of proportionality between BCG cell concentration and magnetoresistive voltage signal; iii) application of multiplicative signal correction for systematic effects removal; iv) investigation of calibration effectiveness using chemometrics methods; and v) comparison with state-of-the-art point-of-care tuberculosis biosensors. Results present a clear correspondence between voltage signal and cell concentration. Multiplicative signal correction removes baseline shifts within and between biochip sensors, allowing accurate and precise voltage signal between different biochips. The corrected signal was used for multivariate regression models, which significantly decreased the calibration standard error from 0.50 to 0.03log10 (cells/mL). Results show that Ziehl-Neelsen detection limits and below are achievable with the magnetoresistive biochip, when pre-processing and chemometrics are used. |
|---|---|
| Autores principais: | Barroso, Teresa Raquel Guerra |
| Outros Autores: | Martins, Rui C.; Fernandes, Elisabete; Cardoso, Susana; Rivas, José; Freitas, Paulo P. |
| Assunto: | Animals Biosensing Techniques Cattle Equipment Design Humans Limit of Detection Magnetic Fields Magnetite Nanoparticles Mycobacterium Mycobacterium bovis Mycobacterium tuberculosis Point-of-Care Systems Sputum Tuberculosis Tuberculosis, Bovine Lab-On-A-Chip Devices Nanotechnology Magnetic nanoparticles Magnetoresistive biosensor Chemometrics |
| Ano: | 2018 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866877184033947648 |
|---|---|
| author | Barroso, Teresa Raquel Guerra |
| author2 | Martins, Rui C. Fernandes, Elisabete Cardoso, Susana Rivas, José Freitas, Paulo P. |
| author2_role | author author author author author |
| author_facet | Barroso, Teresa Raquel Guerra Martins, Rui C. Fernandes, Elisabete Cardoso, Susana Rivas, José Freitas, Paulo P. |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Barroso, Teresa Raquel Guerra\"},{\"Person.name\":\"Martins, Rui C.\"},{\"Person.name\":\"Fernandes, Elisabete\"},{\"Person.name\":\"Cardoso, Susana\"},{\"Person.name\":\"Rivas, José\"},{\"Person.name\":\"Freitas, Paulo P.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Barroso, Teresa Raquel Guerra Martins, Rui C. Fernandes, Elisabete Cardoso, Susana Rivas, José Freitas, Paulo P. |
| datacite.date.Accepted.fl_str_mv | 2018-02-15T00:00:00Z |
| datacite.date.available.fl_str_mv | 2020-02-15T07:00:16Z |
| datacite.date.embargoed.fl_str_mv | 2020-02-15T07:00:16Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Animals Biosensing Techniques Cattle Equipment Design Humans Limit of Detection Magnetic Fields Magnetite Nanoparticles Mycobacterium Mycobacterium bovis Mycobacterium tuberculosis Point-of-Care Systems Sputum Tuberculosis Tuberculosis, Bovine Lab-On-A-Chip Devices Nanotechnology Magnetic nanoparticles Magnetoresistive biosensor Chemometrics |
| datacite.titles.title.fl_str_mv | Detection of BCG bacteria using a magnetoresistive biosensor: A step towards a fully electronic platform for tuberculosis point-of-care detection |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Barroso, Teresa Raquel Guerra Martins, Rui C. Fernandes, Elisabete Cardoso, Susana Rivas, José Freitas, Paulo P. |
| dc.date.Accepted.fl_str_mv | 2018-02-15T00:00:00Z |
| dc.date.available.fl_str_mv | 2020-02-15T07:00:16Z |
| dc.date.embargoed.fl_str_mv | 2020-02-15T07:00:16Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/58042 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Animals Biosensing Techniques Cattle Equipment Design Humans Limit of Detection Magnetic Fields Magnetite Nanoparticles Mycobacterium Mycobacterium bovis Mycobacterium tuberculosis Point-of-Care Systems Sputum Tuberculosis Tuberculosis, Bovine Lab-On-A-Chip Devices Nanotechnology Magnetic nanoparticles Magnetoresistive biosensor Chemometrics |
| dc.title.fl_str_mv | Detection of BCG bacteria using a magnetoresistive biosensor: A step towards a fully electronic platform for tuberculosis point-of-care detection |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Tuberculosis is one of the major public health concerns. This highly contagious disease affects more than 10.4 million people, being a leading cause of morbidity by infection. Tuberculosis is diagnosed at the point-of-care by the Ziehl-Neelsen sputum smear microscopy test. Ziehl-Neelsen is laborious, prone to human error and infection risk, with a limit of detection of 104 cells/mL. In resource-poor nations, a more practical test, with lower detection limit, is paramount. This work uses a magnetoresistive biosensor to detect BCG bacteria for tuberculosis diagnosis. Herein we report: i) nanoparticle assembly method and specificity for tuberculosis detection; ii) demonstration of proportionality between BCG cell concentration and magnetoresistive voltage signal; iii) application of multiplicative signal correction for systematic effects removal; iv) investigation of calibration effectiveness using chemometrics methods; and v) comparison with state-of-the-art point-of-care tuberculosis biosensors. Results present a clear correspondence between voltage signal and cell concentration. Multiplicative signal correction removes baseline shifts within and between biochip sensors, allowing accurate and precise voltage signal between different biochips. The corrected signal was used for multivariate regression models, which significantly decreased the calibration standard error from 0.50 to 0.03log10 (cells/mL). Results show that Ziehl-Neelsen detection limits and below are achievable with the magnetoresistive biochip, when pre-processing and chemometrics are used. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/64fb4b49-37fc-415f-a971-1629e6be979a/download |
| id | rum_6157761be75207dff9a1ea69efbd0421 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/58042 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/58042 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Barroso, Teresa Raquel Guerra Martins, Rui C. Fernandes, Elisabete Cardoso, Susana Rivas, José Freitas, Paulo P. |
| publishDate | 2018 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engElsevierporTuberculosis is one of the major public health concerns. This highly contagious disease affects more than 10.4 million people, being a leading cause of morbidity by infection. Tuberculosis is diagnosed at the point-of-care by the Ziehl-Neelsen sputum smear microscopy test. Ziehl-Neelsen is laborious, prone to human error and infection risk, with a limit of detection of 104 cells/mL. In resource-poor nations, a more practical test, with lower detection limit, is paramount. This work uses a magnetoresistive biosensor to detect BCG bacteria for tuberculosis diagnosis. Herein we report: i) nanoparticle assembly method and specificity for tuberculosis detection; ii) demonstration of proportionality between BCG cell concentration and magnetoresistive voltage signal; iii) application of multiplicative signal correction for systematic effects removal; iv) investigation of calibration effectiveness using chemometrics methods; and v) comparison with state-of-the-art point-of-care tuberculosis biosensors. Results present a clear correspondence between voltage signal and cell concentration. Multiplicative signal correction removes baseline shifts within and between biochip sensors, allowing accurate and precise voltage signal between different biochips. The corrected signal was used for multivariate regression models, which significantly decreased the calibration standard error from 0.50 to 0.03log10 (cells/mL). Results show that Ziehl-Neelsen detection limits and below are achievable with the magnetoresistive biochip, when pre-processing and chemometrics are used.application/pdfporDetection of BCG bacteria using a magnetoresistive biosensor: A step towards a fully electronic platform for tuberculosis point-of-care detectionBarroso, Teresa Raquel GuerraMartins, Rui C.Fernandes, ElisabeteCardoso, SusanaRivas, JoséFreitas, Paulo P.HostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf0956-5663DOIIsPartOf10.1016/j.bios.2017.09.0042020-02-15T07:00:16Z2018-02-152018-02-15T00:00:00ZHandlehttps://hdl.handle.net/1822/58042http://purl.org/coar/access_right/c_abf2open accessAnimalsBiosensing TechniquesCattleEquipment DesignHumansLimit of DetectionMagnetic FieldsMagnetite NanoparticlesMycobacteriumMycobacterium bovisMycobacterium tuberculosisPoint-of-Care SystemsSputumTuberculosisTuberculosis, BovineLab-On-A-Chip DevicesNanotechnologyMagnetic nanoparticlesMagnetoresistive biosensorChemometrics682742 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/64fb4b49-37fc-415f-a971-1629e6be979a/download |
| spellingShingle | Detection of BCG bacteria using a magnetoresistive biosensor: A step towards a fully electronic platform for tuberculosis point-of-care detection Barroso, Teresa Raquel Guerra Animals Biosensing Techniques Cattle Equipment Design Humans Limit of Detection Magnetic Fields Magnetite Nanoparticles Mycobacterium Mycobacterium bovis Mycobacterium tuberculosis Point-of-Care Systems Sputum Tuberculosis Tuberculosis, Bovine Lab-On-A-Chip Devices Nanotechnology Magnetic nanoparticles Magnetoresistive biosensor Chemometrics |
| status | SINGLETON |
| subject.fl_str_mv | Animals Biosensing Techniques Cattle Equipment Design Humans Limit of Detection Magnetic Fields Magnetite Nanoparticles Mycobacterium Mycobacterium bovis Mycobacterium tuberculosis Point-of-Care Systems Sputum Tuberculosis Tuberculosis, Bovine Lab-On-A-Chip Devices Nanotechnology Magnetic nanoparticles Magnetoresistive biosensor Chemometrics |
| title | Detection of BCG bacteria using a magnetoresistive biosensor: A step towards a fully electronic platform for tuberculosis point-of-care detection |
| title_full | Detection of BCG bacteria using a magnetoresistive biosensor: A step towards a fully electronic platform for tuberculosis point-of-care detection |
| title_fullStr | Detection of BCG bacteria using a magnetoresistive biosensor: A step towards a fully electronic platform for tuberculosis point-of-care detection |
| title_full_unstemmed | Detection of BCG bacteria using a magnetoresistive biosensor: A step towards a fully electronic platform for tuberculosis point-of-care detection |
| title_short | Detection of BCG bacteria using a magnetoresistive biosensor: A step towards a fully electronic platform for tuberculosis point-of-care detection |
| title_sort | Detection of BCG bacteria using a magnetoresistive biosensor: A step towards a fully electronic platform for tuberculosis point-of-care detection |
| topic | Animals Biosensing Techniques Cattle Equipment Design Humans Limit of Detection Magnetic Fields Magnetite Nanoparticles Mycobacterium Mycobacterium bovis Mycobacterium tuberculosis Point-of-Care Systems Sputum Tuberculosis Tuberculosis, Bovine Lab-On-A-Chip Devices Nanotechnology Magnetic nanoparticles Magnetoresistive biosensor Chemometrics |
| topic_facet | Animals Biosensing Techniques Cattle Equipment Design Humans Limit of Detection Magnetic Fields Magnetite Nanoparticles Mycobacterium Mycobacterium bovis Mycobacterium tuberculosis Point-of-Care Systems Sputum Tuberculosis Tuberculosis, Bovine Lab-On-A-Chip Devices Nanotechnology Magnetic nanoparticles Magnetoresistive biosensor Chemometrics |
| url | https://hdl.handle.net/1822/58042 |
| visible | 1 |